| General information | |||
| TC013252 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 11534716..11546899 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013252 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02806 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08364 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC013252 RA
MKQQLTKVL SDDEALINK KLPKELLLR IFSYIDVVS LCRCAQVSK AWNVLALDG SNWQRIDLF DFQKDVEGP IIENISRRC GGFLRQLSL RGCQSIADG SMKTLAQLC PNVEDLNLN GCKKLTDAS CTAFSKHCS KLQKLNLDG CSAITDNSL KALSDGCPN LTHINISWS NNVTENGVE ALARGCRKL KSFISKGCK QITSRAVIC LARFCDQLE VVNLLGCCH ITDEAVQAL AEKCPKLHY LCLSGCSAL TDASLIALA QKCTLLSTL EVAGCSQFT DAGFQALAR SCRYLEKMD LDECVLITD NTLIHLAMG CPRIEYLTL SHCELITDE GIRHLSMSP CAAENLTVL ELDNCPLVT DASLEHLIS CHNLQRVEL YDCQLITRV GIRRLRNHL PNIKVHAYF APVTPPPSA GGSRQRYCR CCVIL
- >TC013252 RB
MVSNCISFQ NTAKKHETS TGRCHRFIP QVPQEFDCR VKANIKREL LNKDLAIRN EEFETWYSK EYPRKVVLP DQLFRPPQY QSLEYLRNV EEKLFVSPP PPIVLPLSE MKDSFRMNY TEPHTDEVK APVVTAKCH VSKLGCEQQ PAIQPHTKG FWKWTDPYL TVYKSEYIP HEGITDDKT LTFYNQAEF ALPPSSGNR TMFDKQIFK NMLPVRLMS RSLKRVPNF GMQSEYQDH YIKQTFSYL YPHLQDRCT LFEDSLSEA SRWQNLPPP GMYCSEYCN IGGFSVRRV VDQSTPTKE IARTKNVAD RAQAALRSI SGGCSVAVG TATIRLDRR RGAPRRLLC VHRPPAVSP PAKTACFCF GRAGTNGGG HKQIPDFSF VDGALQPRP PVLPVPQQY TASARSVSA PQRKRTFRT LCAPITMIY AGRNKLESA RTSPLEGNN ELSATVSNL ARRWWRSKK HSKGSSKET NSLMAVDTM KQQLTKVLS DDEALINKK LPKELLLRI FSYIDVVSL CRCAQVSKA WNVLALDGS NWQRIDLFD FQKDVEGPI IENISRRCG GFLRQLSLR GCQSIADGS MKTLAQLCP NVEDLNLNG CKKLTDASC TAFSKHCSK LQKLNLDGC SAITDNSLK ALSDGCPNL THINISWSN NVTENGVEA LARGCRKLK SFISKGCKQ ITSRAVICL ARFCDQLEV VNLLGCCHI TDEAVQALA EKCPKLHYL CLSGCSALT DASLIALAQ KCTLLSTLE VAGCSQFTD AGFQALARS CRYLEKMDL DECVLITDN TLIHLAMGC PRIEYLTLS HCELITDEG IRHLSMSPC AAENLTVLE LDNCPLVTD ASLEHLISC HNLQRVELY DCQLITRVG IRRLRNHLP NIKVHAYFA PVTPPPSAG GSRQRYCRC CVIL
- >TC013252 RC
MVSNCISFQ NTAKKHETS TGRCHRFIP QVPQEFDCR VKANIKREL LNKDLAIRN EEFETWYSK EYPRKVVLP DQLFRPPQY QSLEYLRNV EEKLFVSPP PPIVLPLSE MKDSFRMNY TEPHTDEVK APVVTAKCH VSKLGCEQQ PAIQPHTKG FWKWTDPYL TVYKSEYIP HEGITDDKT LTFYNQAEF ALPPSSGNR TMFDKQIFK NMLPVRLMS RSLKRVPNF GMQSEYQDH YIKQTFSYL YPHLQDRCT LFEDSLSEA SRWQNLPPP GMYCSEYCN IGGFSVRRV VDQSTPTKE IARTKNAHA ASPIERKQR SDRYPVVAA SPSAPPPFV SIGVAALQG DFFVSIDPQ QSRRPRKRL VFVLVVPAP MVGVINKSP TSHSSTERS SQGRQYYQS RNSTPPVHV L
- >TC013252 RA
atgaaacaa cagttaact aaagtgttg tctgatgac gaagccctc attaacaag aaattgcca aaggagctc ctgcttcgg attttctcc tacatagac gtagtgtcg ttgtgtcgg tgtgcccaa gtgtctaaa gcatggaat gtcctggct ttagacggc tcaaactgg caaagaatc gacttgttt gactttcag aaagacgta gagggccct attatcgaa aatatatct cgaagatgt ggcgggttt ctgcgccaa ttgtcactg cgtggctgc cagtctatc gcagacggc tccatgaaa accctagct caactgtgc ccaaacgtg gaagattta aatctcaac ggttgtaaa aaactgact gatgcctcc tgcacggct ttttctaaa cactgttct aaacttcag aaacttaac ttagacggt tgttcggca atcaccgac aattccctg aaagcgttg agtgacggt tgtccgaat ttgactcac atcaacatt agctggtcg aacaacgtt acagaaaac ggcgtggag gcgctagcg cgagggtgc cgaaaactc aaaagcttt atcagtaaa gggtgtaaa cagattact tctcgagct gttatttgt ctagctagg ttctgcgat caattagaa gttgttaat ttgcttggt tgttgtcac ataaccgac gaggctgtg caggctttg gctgaaaag tgtccaaag ttgcactat ctgtgtctt tcaggatgt tcggcattg acggacgct tcgctcata gcgttggct caaaaatgt acgcttttg tcaacgctt gaagtcgct ggatgctcg cagttcact gatgccggt tttcaagca cttgccaga agctgccgt tacctcgag aaaatggac ttggacgaa tgtgttttg ataacggat aataccctg atacactta gccatgggt tgcccacgg attgaatat ttgactctc tctcactgc gagctgatc acggacgag ggcataaga cacttgtcg atgtccccg tgcgccgca gaaaacctc accgtttta gaattagac aattgccca ctggttacc gacgcttcc ctcgaacac ttaatatca tgccataat ttacaacga gtcgaactt tatgattgt cagctcatc acacgggtt ggaattaga cgattaaga aaccattta cccaatatt aaagtccac gcttacttt gcaccagtt acgccccca ccttcggcc ggaggatcc cgtcaaagg tattgtagg tgctgtgta attctatga
- >TC013252 RB
atggtttca aattgtatc agctttcaa aacacagca aaaaaacat gagacgtcc accggaagg tgtcaccgg tttatcccg caagtgccc caagaattt gattgtcga gtcaaagca aacattaag cgcgaactg ctaaacaaa gaccttgca atcagaaat gaggaattc gagacgtgg tactccaag gagtatccg cggaaagtc gttttaccg gaccagcta ttcaggccg ccacaatac caaagcttg gagtatctg aggaatgtg gaggaaaaa ttgtttgtt agccctccc cctccgatt gtgctccct ctctccgaa atgaaggac tcgttccga atgaattac actgagccc cacacagac gaagtcaaa gcacctgtt gttacagca aaatgccat gtgagcaag ctgggctgt gagcaacaa ccagccatc cagcctcac accaagggc ttttggaag tggacagac ccttacttg accgtctac aagtccgaa tatatcccc catgaaggc ataactgac gataaaaca ctaacattc tataaccaa gccgagttc gctttgcct ccatcttcc ggcaacaga acaatgttt gataaacaa atattcaag aatatgctg ccggtgcgg ctcatgtcg aggtcgttg aaacgcgtc cccaatttt ggcatgcaa tcggagtac caggatcac tacatcaaa cagaccttc tcgtatctc tacccccat ctccaggac cgatgcact ctgttcgag gattcgctg tctgaggcg agtcggtgg cagaacttg ccacccccg ggaatgtat tgctctgag tactgcaat attgggggt ttttcggtc cggcgggtc gtagatcaa agcacaccg accaaagaa atagcgcga acgaagaac gtcgccgat cgagcgcaa gcagcgctc cgatcgata tccggtggt tgcagcgtc gccgtcggc accgccacc attcgtctc gatcggcgt cgcggcgct ccaaggcga cttctttgt gtccatcga cccccagca gtctcgccg cccgcgaaa acggcttgt ttttgtttt ggtcgtgcc ggcaccaat ggtgggggt cataaacaa atccccgac ttctcattc gtcgacgga gcgctccag ccaaggccg ccagtatta ccagtcccg caacagtac accgccagt gcacgttct gtgagtgcg ccccagcga aaacgcact tttcgcaca ctttgcgcc cccattact atgatttat gcgggtagg aacaagctt gagtctgct cgcacatca cctctggaa gggaataat gaactaagt gcaactgtt tctaatcta gcacgccgt tggtggcga agtaaaaaa cattctaaa ggctctagt aaagaaacc aattcacta atggctgta gatacgatg aaacaacag ttaactaaa gtgttgtct gatgacgaa gccctcatt aacaagaaa ttgccaaag gagctcctg cttcggatt ttctcctac atagacgta gtgtcgttg tgtcggtgt gcccaagtg tctaaagca tggaatgtc ctggcttta gacggctca aactggcaa agaatcgac ttgtttgac tttcagaaa gacgtagag ggccctatt atcgaaaat atatctcga agatgtggc gggtttctg cgccaattg tcactgcgt ggctgccag tctatcgca gacggctcc atgaaaacc ctagctcaa ctgtgccca aacgtggaa gatttaaat ctcaacggt tgtaaaaaa ctgactgat gcctcctgc acggctttt tctaaacac tgttctaaa cttcagaaa cttaactta gacggttgt tcggcaatc accgacaat tccctgaaa gcgttgagt gacggttgt ccgaatttg actcacatc aacattagc tggtcgaac aacgttaca gaaaacggc gtggaggcg ctagcgcga gggtgccga aaactcaaa agctttatc agtaaaggg tgtaaacag attacttct cgagctgtt atttgtcta gctaggttc tgcgatcaa ttagaagtt gttaatttg cttggttgt tgtcacata accgacgag gctgtgcag gctttggct gaaaagtgt ccaaagttg cactatctg tgtctttca ggatgttcg gcattgacg gacgcttcg ctcatagcg ttggctcaa aaatgtacg cttttgtca acgcttgaa gtcgctgga tgctcgcag ttcactgat gccggtttt caagcactt gccagaagc tgccgttac ctcgagaaa atggacttg gacgaatgt gttttgata acggataat accctgata cacttagcc atgggttgc ccacggatt gaatatttg actctctct cactgcgag ctgatcacg gacgagggc ataagacac ttgtcgatg tccccgtgc gccgcagaa aacctcacc gttttagaa ttagacaat tgcccactg gttaccgac gcttccctc gaacactta atatcatgc cataattta caacgagtc gaactttat gattgtcag ctcatcaca cgggttgga attagacga ttaagaaac catttaccc aatattaaa gtccacgct tactttgca ccagttacg cccccacct tcggccgga ggatcccgt caaaggtat tgtaggtgc tgtgtaatt ctatga
- >TC013252 RC
atggtttca aattgtatc agctttcaa aacacagca aaaaaacat gagacgtcc accggaagg tgtcaccgg tttatcccg caagtgccc caagaattt gattgtcga gtcaaagca aacattaag cgcgaactg ctaaacaaa gaccttgca atcagaaat gaggaattc gagacgtgg tactccaag gagtatccg cggaaagtc gttttaccg gaccagcta ttcaggccg ccacaatac caaagcttg gagtatctg aggaatgtg gaggaaaaa ttgtttgtt agccctccc cctccgatt gtgctccct ctctccgaa atgaaggac tcgttccga atgaattac actgagccc cacacagac gaagtcaaa gcacctgtt gttacagca aaatgccat gtgagcaag ctgggctgt gagcaacaa ccagccatc cagcctcac accaagggc ttttggaag tggacagac ccttacttg accgtctac aagtccgaa tatatcccc catgaaggc ataactgac gataaaaca ctaacattc tataaccaa gccgagttc gctttgcct ccatcttcc ggcaacaga acaatgttt gataaacaa atattcaag aatatgctg ccggtgcgg ctcatgtcg aggtcgttg aaacgcgtc cccaatttt ggcatgcaa tcggagtac caggatcac tacatcaaa cagaccttc tcgtatctc tacccccat ctccaggac cgatgcact ctgttcgag gattcgctg tctgaggcg agtcggtgg cagaacttg ccacccccg ggaatgtat tgctctgag tactgcaat attgggggt ttttcggtc cggcgggtc gtagatcaa agcacaccg accaaagaa atagcgcga acgaagaat gcacatgca gcgtcgccg atcgagcgc aagcagcgc tccgatcga tatccggtg gttgcagcg tcgccgtcg gcaccgcca ccattcgtc tcgatcggc gtcgcggcg ctccaaggc gacttcttt gtgtccatc gacccccag cagtctcgc cgcccgcga aaacggctt gtttttgtt ttggtcgtg ccggcacca atggtgggg gtcataaac aaatccccg acttctcat tcgtcgacg gagcgctcc agccaaggc cgccagtat taccagtcc cgcaacagt acaccgcca gtgcacgtt ctgtga
- >TC013252 RA
acgattttc aacgtgaaa aacagactg ggagtaaac caacaacac tggacaggt gttagcatg taaagtaaa agtgtaaaa gtgaatata tttttatca atgaaagta cgaataatt tcagtgcga aattagaac atttaaatt agctcatac tgcgtttag aaaattatt agggttatt ctaggcgtc atgtttatt gtataaaaa ttccaggaa aagtgattc gtatacgtc cgaaaaggt ttacttagt cgtattctt ggtactcgt ttccggtcg ataaattaa aggctcgat taaacatgt acatttcac tacacactc atgtatttg aaaacaata agctgaatt tgttttgtt attttatat tccgcggca cagtcagat caatttgta atagaaaac ctccacctt acaaagaaa taacgagtt aggaatgca atttagcac ttttacgac catctggaa ccattacat attcactag ttgccacct tatcaaaat taacgctaa attatttca ggtcacttg actaacttt ggacgtagt ttttcgtcc acaaattta acaacagcc taatattgt tgagtggtg aatttgtgg cgaattgaa aggtttgtg aattttata ctagactgt ctttttcat ttacacaat tcaacacaa gacattggt gtgtagctg cgttctcgg atgacttgc gaatgtgtg gtgtgtgtt tctatcact ttatttcat ctggaaata ttttcagat ccaggtaaa ttacgataa ttatacgag ttcttgcat caggacttc accgcttgt cattcttcc aaacaacgc gatataagt tctgtcaaa gtactaaca aagttttat aaaattata ggtgttgtc agtcttgta acataaaac tttatccgt ccgatgcag agtataaaa tcaatacct gtcggctgc agcgttatt atattaaaa aacaactag ttagaaaga atttaattt ccaattacc agaaaaatt cgggaaatg gcgagtttc gctgaaaca tttattacc cggagtgag tcaagtttt tgcagctgt tggaaaccg gaagagtta attagagaa atgatttac aattatttg agaacgata aactagaat tctgggttt gaggtgata acgtatcta aaacaatca aaagggtgt tgcatgagc aaataccga atttcgaaa ttttgctct cagttctgt gtgcacttt attttattg atgtaatga tgtaattgc gttttttaa taaatgatt taataataa tcacagtac aggcaagtg ctgtttttt cttccaccc actattttc ccctttttc atttttact aacaattcg cacggttag tgactggga aaggtatag ccacctcat ttttattta atcctcgtt atcgccaca tttagtagc gtcacaaac atcagaaac gtatttatt tcgtaagcc atttatttg tcgattttt ttaagtttt ttgaatgtc tttattttg catgttagt ctgctcgca catcacctc tggaaggga ataatgaac taagtgcaa ctgtttcta atctagcac gccgttggt ggcgaagta aaaaacatt ctaaaggct ctagtaaag aaaccaatt cactaatgg ctgtagata cgatgaaac aacagttaa ctaaagtgt tgtctgatg acgaagccc tcattaaca agaaattgc caaaggagc tcctgcttc ggattttct cctacatag acgtagtgt cgttgtgtc ggtgtgccc aagtgtcta aagcatgga atgtcctgg ctttagacg gctcaaact ggcaaagaa tcgacttgt ttgactttc agaaagacg tagagggcc ctattatcg aaaatatat ctcgaagat gtggcgggt ttctgcgcc aattgtcac tgcgtggct gccagtcta tcgcagacg gctccatga aaaccctag ctcaactgt gcccaaacg tggaagatt taaatctca acggttgta aaaaactga ctgatgcct cctgcacgg ctttttcta aacactgtt ctaaacttc agaaactta acttagacg gttgttcgg caatcaccg acaattccc tgaaagcgt tgagtgacg gttgtccga atttgactc acatcaaca ttagctggt cgaacaacg ttacagaaa acggcgtgg aggcgctag cgcgagggt gccgaaaac tcaaaagct ttatcagta aagggtgta aacagatta cttctcgag ctgttattt gtctagcta ggttctgcg atcaattag aagttgtta atttgcttg gttgttgtc acataaccg acgaggctg tgcaggctt tggctgaaa agtgtccaa agttgcact atctgtgtc tttcaggat gttcggcat tgacggacg cttcgctca tagcgttgg ctcaaaaat gtacgcttt tgtcaacgc ttgaagtcg ctggatgct cgcagttca ctgatgccg gttttcaag cacttgcca gaagctgcc gttacctcg agaaaatgg acttggacg aatgtgttt tgataacgg ataataccc tgatacact tagccatgg gttgcccac ggattgaat atttgactc tctctcact gcgagctga tcacggacg agggcataa gacacttgt cgatgtccc cgtgcgccg cagaaaacc tcaccgttt tagaattag acaattgcc cactggtta ccgacgctt ccctcgaac acttaatat catgccata atttacaac gagtcgaac tttatgatt gtcagctca tcacacggg ttggaatta gacgattaa gaaaccatt tacccaata ttaaagtcc acgcttact ttgcaccag ttacgcccc caccttcgg ccggaggat cccgtcaaa ggtattgta ggtgctgtg taattctat gagaaaatg atccccttg gctccaatg atatgccac cgtcataat gctatgtgt tcagcacgc ttcacgtga cactacaat aatacgtat tacgctact agggatctt acaaaacga ttgcgcttt ttcaacaca ctttcagca ttcgtttct taatatcga atcgttaac gtgaaattc gtgacaatc gagctttaa aatgtgatt cgagttcca aaacaagcg actgatttg atatttgga aactgactt agctagttc tgtttgcaa aattcactc atgcaaatt aatcgtttc tgtttttgc gcagttgtt gagtgagac ttcagccat cgcgttaaa aatgttcaa aaactttgt taagtagtg attttgtat gaaaagtac ttacttgtt ggaaattgt gtaaatgtg ctcaaataa gtttttgtg acatttttc aacctagtt attggatag tgcatttga tatgtattg gggccataa ggggaaatt cgattcaaa atgtaatct ctcaatatc tcacggtta ctcttattt gaaagttaa gttattgtt agacttatt ttaatttta tttatgcat aatgttggg tgctattag tgtaaactt ttgttacgt gttgttact gcggggttg tgctgcttc cagattatc tatgcaatt tcaaacgtt gaacagtct ttaactaga gcatgacag cgcatctca gcaccgaga ttactacac tagccattg tttgttact gttatcaga aagttgcgt gatatttat ttaatatcg attttagtg ataattctt ttacttcgt tgattggat attatagac gaaatattt acaaaatgt atagtcaac agcatttct aaatgaaag tataacatg tagactttt agtgatttt aatgtttga acaatatcc aaacttaac aagtatatt aaccctata actgatgga ttttgggca gtaatattt cggtacgaa aacatttgt acccaagta ttacgtttt cttcttata tcaatatca gaccaacga ttctgtaat tatctacgt cttacaccc gttattact ttgcagtct ttattggtc gcctcaaat tgcctgtga taaagaaat taaatcccg attgttatt tttatttat tggaaagtt acttatgat ttaacaaat tctttcgga ccgttgatt ttatttttg cgtattgtc accatcagt tacgaaata tgtttgagt tgagagaat atttcaatg attttgttt gggacaatt gatagagaa tgtttacca ccatatttt tgtatttaa ctatttata ggtgttata tgttgtgtt atacacaaa atatagtat gtatattat gtcacttac ctatgatat aacttagtt ttaagaaaa aaagtaata tgtaggttt ttattagcc ctggcggca tttaagtat aggaatatt tattttaca aaagactga gcacgagct attagaaat ttttattaa ttgtactgt atgtgcatt tttaataaa cacttatgt attat
- >TC013252 RB
ggaaggtta gaagtaaaa tggtttcaa attgtatca gctttcaaa acacagcaa aaaaacatg agacgtcca ccggaaggt gtcaccggt ttatcccgc aagtgcccc aagaatttg attgtcgag tcaaagcaa acattaagc gcgaactgc taaacaaag accttgcaa tcagaaatg aggaattcg agacgtggt actccaagg agtatccgc ggaaagtcg ttttaccgg accagctat tcaggccgc cacaatacc aaagcttgg agtatctga ggaatgtgg aggaaaaat tgtttgtta gccctcccc ctccgattg tgctccctc tctccgaaa tgaaggact cgttccgaa tgaattaca ctgagcccc acacagacg aagtcaaag cacctgttg ttacagcaa aatgccatg tgagcaagc tgggctgtg agcaacaac cagccatcc agcctcaca ccaagggct tttggaagt ggacagacc cttacttga ccgtctaca agtccgaat atatccccc atgaaggca taactgacg ataaaacac taacattct ataaccaag ccgagttcg ctttgcctc catcttccg gcaacagaa caatgtttg ataaacaaa tattcaaga atatgctgc cggtgcggc tcatgtcga ggtcgttga aacgcgtcc ccaattttg gcatgcaat cggagtacc aggatcact acatcaaac agaccttct cgtatctct acccccatc tccaggacc gatgcactc tgttcgagg attcgctgt ctgaggcga gtcggtggc agaacttgc cacccccgg gaatgtatt gctctgagt actgcaata ttgggggtt tttcggtcc ggcgggtcg tagatcaaa gcacaccga ccaaagaaa tagcgcgaa cgaagaacg tcgccgatc gagcgcaag cagcgctcc gatcgatat ccggtggtt gcagcgtcg ccgtcggca ccgccacca ttcgtctcg atcggcgtc gcggcgctc caaggcgac ttctttgtg tccatcgac ccccagcag tctcgccgc ccgcgaaaa cggcttgtt tttgttttg gtcgtgccg gcaccaatg gtgggggtc ataaacaaa tccccgact tctcattcg tcgacggag cgctccagc caaggccgc cagtattac cagtcccgc aacagtaca ccgccagtg cacgttctg tgagtgcgc cccagcgaa aacgcactt ttcgcacac tttgcgccc ccattacta tgatttatg cgggtagga acaagcttg agtctgctc gcacatcac ctctggaag ggaataatg aactaagtg caactgttt ctaatctag cacgccgtt ggtggcgaa gtaaaaaac attctaaag gctctagta aagaaacca attcactaa tggctgtag atacgatga aacaacagt taactaaag tgttgtctg atgacgaag ccctcatta acaagaaat tgccaaagg agctcctgc ttcggattt tctcctaca tagacgtag tgtcgttgt gtcggtgtg cccaagtgt ctaaagcat ggaatgtcc tggctttag acggctcaa actggcaaa gaatcgact tgtttgact ttcagaaag acgtagagg gccctatta tcgaaaata tatctcgaa gatgtggcg ggtttctgc gccaattgt cactgcgtg gctgccagt ctatcgcag acggctcca tgaaaaccc tagctcaac tgtgcccaa acgtggaag atttaaatc tcaacggtt gtaaaaaac tgactgatg cctcctgca cggcttttt ctaaacact gttctaaac ttcagaaac ttaacttag acggttgtt cggcaatca ccgacaatt ccctgaaag cgttgagtg acggttgtc cgaatttga ctcacatca acattagct ggtcgaaca acgttacag aaaacggcg tggaggcgc tagcgcgag ggtgccgaa aactcaaaa gctttatca gtaaagggt gtaaacaga ttacttctc gagctgtta tttgtctag ctaggttct gcgatcaat tagaagttg ttaatttgc ttggttgtt gtcacataa ccgacgagg ctgtgcagg ctttggctg aaaagtgtc caaagttgc actatctgt gtctttcag gatgttcgg cattgacgg acgcttcgc tcatagcgt tggctcaaa aatgtacgc ttttgtcaa cgcttgaag tcgctggat gctcgcagt tcactgatg ccggttttc aagcacttg ccagaagct gccgttacc tcgagaaaa tggacttgg acgaatgtg ttttgataa cggataata ccctgatac acttagcca tgggttgcc cacggattg aatatttga ctctctctc actgcgagc tgatcacgg acgagggca taagacact tgtcgatgt ccccgtgcg ccgcagaaa acctcaccg ttttagaat tagacaatt gcccactgg ttaccgacg cttccctcg aacacttaa tatcatgcc ataatttac aacgagtcg aactttatg attgtcagc tcatcacac gggttggaa ttagacgat taagaaacc atttaccca atattaaag tccacgctt actttgcac cagttacgc ccccacctt cggccggag gatcccgtc aaaggtatt gtaggtgct gtgtaattc tatgagaaa atgatcccc ttggctcca atgatatgc caccgtcat aatgctatg tgttcagca cgcttcacg tgacactac aataatacg tattacgct actagggat cttacaaaa cgattgcgc tttttcaac acactttca gcattcgtt tcttaatat cgaatcgtt aacgtgaaa ttcgtgaca atcgagctt taaaatgtg attcgagtt ccaaaacaa gcgactgat ttgatattt ggaaactga cttagctag ttctgtttg caaaattca ctcatgcaa attaatcgt ttctgtttt tgcgcagtt gttgagtga gacttcagc catcgcgtt aaaaatgtt caaaaactt tgttaagta gtgattttg tatgaaaag tacttactt gttggaaat tgtgtaaat gtgctcaaa taagttttt gtgacattt ttcaaccta gttattgga tagtgcatt tgatatgta ttggggcca taaggggaa attcgattc aaaatgtaa tctctcaat atctcacgg ttactctta tttgaaagt taagttatt gttagactt attttaatt ttatttatg cataatgtt gggtgctat tagtgtaaa cttttgtta cgtgttgtt actgcgggg ttgtgctgc ttccagatt atctatgca atttcaaac gttgaacag tctttaact agagcatga cagcgcatc tcagcaccg agattacta cactagcca ttgtttgtt actgttatc agaaagttg cgtgatatt tatttaata tcgatttta gtgataatt cttttactt cgttgattg gatattata gacgaaata tttacaaaa tgtatagtc aacagcatt tctaaatga aagtataac atgtagact tttagtgat tttaatgtt tgaacaata tccaaactt aacaagtat attaaccct ataactgat ggattttgg gcagtaata tttcggtac gaaaacatt tgtacccaa gtattacgt tttcttctt atatcaata tcagaccaa cgattctgt aattatcta cgtcttaca cccgttatt actttgcag tctttattg gtcgcctca aattgcctg tgataaaga aattaaatc ccgattgtt atttttatt tattggaaa gttacttat gatttaaca aattctttc ggaccgttg attttattt ttgcgtatt gtcaccatc agttacgaa atatgtttg agttgagag aatatttca atgattttg tttgggaca attgataga gaatgttta ccaccatat ttttgtatt taactattt ataggtgtt atatgttgt gttatacac aaaatatag tatgtatat tatgtcact tacctatga tataactta gttttaaga aaaaaagta atatgtagg tttttatta gccctggcg gcatttaag tataggaat atttatttt acaaaagac tgagcacga gctattaga aatttttat taattgtac tgtatgtgc atttttaat aaacactta tgtattat
- >TC013252 RC
ggaaggtta gaagtaaaa tggtttcaa attgtatca gctttcaaa acacagcaa aaaaacatg agacgtcca ccggaaggt gtcaccggt ttatcccgc aagtgcccc aagaatttg attgtcgag tcaaagcaa acattaagc gcgaactgc taaacaaag accttgcaa tcagaaatg aggaattcg agacgtggt actccaagg agtatccgc ggaaagtcg ttttaccgg accagctat tcaggccgc cacaatacc aaagcttgg agtatctga ggaatgtgg aggaaaaat tgtttgtta gccctcccc ctccgattg tgctccctc tctccgaaa tgaaggact cgttccgaa tgaattaca ctgagcccc acacagacg aagtcaaag cacctgttg ttacagcaa aatgccatg tgagcaagc tgggctgtg agcaacaac cagccatcc agcctcaca ccaagggct tttggaagt ggacagacc cttacttga ccgtctaca agtccgaat atatccccc atgaaggca taactgacg ataaaacac taacattct ataaccaag ccgagttcg ctttgcctc catcttccg gcaacagaa caatgtttg ataaacaaa tattcaaga atatgctgc cggtgcggc tcatgtcga ggtcgttga aacgcgtcc ccaattttg gcatgcaat cggagtacc aggatcact acatcaaac agaccttct cgtatctct acccccatc tccaggacc gatgcactc tgttcgagg attcgctgt ctgaggcga gtcggtggc agaacttgc cacccccgg gaatgtatt gctctgagt actgcaata ttgggggtt tttcggtcc ggcgggtcg tagatcaaa gcacaccga ccaaagaaa tagcgcgaa cgaagaatg cacatgcag cgtcgccga tcgagcgca agcagcgct ccgatcgat atccggtgg ttgcagcgt cgccgtcgg caccgccac cattcgtct cgatcggcg tcgcggcgc tccaaggcg acttctttg tgtccatcg acccccagc agtctcgcc gcccgcgaa aacggcttg tttttgttt tggtcgtgc cggcaccaa tggtggggg tcataaaca aatccccga cttctcatt cgtcgacgg agcgctcca gccaaggcc gccagtatt accagtccc gcaacagta caccgccag tgcacgttc tgtgagtgc gccccagcg aaaacgcac ttttcgcac actttgcgc ccccattac tatgattta tgcgggtag gaacaagct tgaggtatg ttggcagag cgtagttgt gtgaacgcg cccgaaatc acgttgttt ttattcacg tccagccgt ttgatgtaa atcagctga ttgtgtaaa cgtcaactt gggtatttc gttgcgatt ttttggcac accgcatga gcacggccg tttttttgc tggtttacg cggattgtt tttgcgttt ttttctttt tggtgtgtt gtctgtggc aaatacgat tgtttggat ttttttttc tttgttcac gagtccgat tcctactat tcgtcgcaa acatgtccg ccgtttaat gacatctct ccaattctg cgtcttaaa tttttcacg gacttgttc aatgttccg cgtttttat tattcacac atttaacga attattttt gtttgtggt cgttaccac tgaaagact gcacatttt tgccaaatc agactggtc ttcgtcact cttcatgaa tgttttatt tttggctca attctattt tgcttcggg aagaataac aaatagaat cgataacaa ttcgtaatt tgagccact cactcattc gaaaagtac tattttctc gctcctttg cttaataac gagaaattc tgtaattgt ttgctttga aatgtgctt tttctggta caaatataa ctagaaata taat
