Status
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Gene information
General information
TC031355
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030231,TC030230
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2139902..2146310
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031355
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08363

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031355 RA
      MPQVKLHKH YVPIILLSK ILGIAPISY KNSNVNYSK 
      IGIVQTVAY FVIFIILTI VFFEKRSAL SPFELIQIR 
      TINFVATFR TIANIILMA VLFLGTFIG RAKFLAILK 
      QMRNLEPEF LKLDQLGAT VRNNRQIRR MVVFLIGVS 
      LSFNLFGDL LTLFIKEEL DLVWREVCT FVVQVYPRL 
      VVNTINLTF LTLLMILEG RFRVINDGI QALINDSRK 
      VTRFPTELT FCNKIKHLV NLHKNLVKV AKDHNSLYS 
      LHLLLWITV TFILLVGDS YIVMYVFFF HLSDVRYVM 
      VVYLLKNVV LYGLELFIL AKIVTDLCQ EANSTKKLI 
      VKIKIDIDK EDERNEVIS SALKLSQNE LEITACKFF 
      SIDNALLFS VIQKKLIVC LLKNVVLYA LEFFILAKI 
      VTDLCQEAN STKKLIVKI RIDIDKEDE RNEVISSAL 
      KLIQNELEI TACKFFSID NALLFSVKK VIESQKNQK 
      SVDLTFCET LTDLIQIHK ILTKITGKI NAVFALQLL 
      LWIGFNFLF LLMDSYTTA HLIFFDWKS SKLYPVSLM 
      LLNVFSYCF YIFYLSRRC ERVCFEANR TAFIVAGTS 
      IDFHKEEER NLIITGILN LMKNKLELT ACRLFSIDN 
      TLLFSVCGA ASSYLFIML QFDLEAFKN LKTRNSTNS 
      SF
    CDS sequence
    • >TC031355 RA
      atgcctcaa gtcaaactc cacaaacat tatgtcccc 
      atcatcctt ttatcaaaa atcctcgga attgcacca 
      atcagctac aaaaacagt aatgtaaac tattcaaaa 
      attggaatc gtccaaact gttgcctac tttgtgatt 
      ttcataatt ttgacaatc gtttttttc gaaaaacga 
      tcggctttg agtccgttc gaattgatc caaattcgc 
      acaattaac ttcgttgcc acgtttcga actatagcc 
      aacatcatc ctcatggcg gtactgttt cttggtact 
      ttcatagga cgagcaaaa tttctggcg attttgaaa 
      caaatgcgt aatttggaa ccggagttt ctcaaactg 
      gaccaactt ggtgcaact gttcgcaac aatagacaa 
      attcgaaga atggtggtg ttcctgatt ggtgtctcg 
      ttgagtttc aatttgttc ggtgatctt ctcacactt 
      tttataaaa gaagaactg gacttggtt tggagggaa 
      gtttgcact tttgtggta caagtctac cctcgactg 
      gttgtaaat accatcaat ttaacgttt ttgactctt 
      ttgatgatt ctggaaggt cgctttaga gtcattaat 
      gacggaatc caagccctc attaatgac tctcgcaaa 
      gtgacacgg tttccaacc gaattgacc ttttgcaac 
      aaaatcaaa catctggtc aaccttcac aaaaacttg 
      gtcaaagtc gccaaagat cacaattcg ctttattcc 
      ttgcatctg ttactttgg attactgtg acttttatt 
      cttcttgtc ggtgactcc tatattgtc atgtatgta 
      ttttttttc cacttatca gatgttcgc tatgttatg 
      gttgtgtac ttgttgaaa aatgtggtc ctgtatggt 
      ttagaattg tttattttg gcgaaaatt gtgaccgac 
      ttgtgtcaa gaagcgaat agtaccaaa aaattaatt 
      gtgaaaatt aaaatcgac atcgacaag gaggatgaa 
      aggaatgag gtcatttca agtgctttg aaactgagt 
      cagaatgag ttggaaata acagcttgc aagtttttc 
      agtattgat aacgctcta ctattttca gtaattcaa 
      aaaaaattg attgtgtgc ttgttaaaa aatgtggtc 
      ctgtatgct ttggaattt tttattttg gcgaaaatt 
      gtgaccgac ttgtgtcaa gaagcaaat agtaccaaa 
      aaactaatt gtgaaaatt agaattgac atcgacaag 
      gaggatgaa aggaatgag gtcatttca agtgctttg 
      aaactgatt cagaatgag ttggagata acagcttgc 
      aagtttttc agcattgat aacgctcta ctattttca 
      gtaaaaaaa gtcattgaa tcacaaaag aaccagaaa 
      tcagtcgat ttaaccttt tgcgaaact ttgaccgat 
      ttgatccaa attcacaaa attttgact aaaatcact 
      ggaaaaatc aacgcggtt tttgcttta caacttttg 
      ttgtggatc ggcttcaac tttctgttc ctcctgatg 
      gattcttac actacggct cacttaatt tttttcgat 
      tggaagtca agtaaattg tatcctgtt tcgctcatg 
      ttattgaat gttttttca tactgtttt tacattttc 
      tacttgtcg agaagatgc gaacgggtc tgttttgaa 
      gcaaaccgc accgctttt atagtggct ggcactagc 
      atcgacttc cacaaagaa gaagaacga aatttgatc 
      atcacagga attttaaat ctgatgaaa aacaaacta 
      gaactaact gcttgtcgc cttttcagt attgacaat 
      actttacta ttttcagtt tgtggtgcc gctagctct 
      tatttattt ataatgcta cagtttgat ttggaggca 
      tttaagaat ctaaaaacc agaaatagt actaattcc 
      agtttctaa
    mRNA sequence
    • >TC031355 RA
      ctagtttat tctagaatc agtaaaagt gataaaagt 
      cataatttt ctgcgtgtt taccggtgt tttttcctt 
      gatgaccca atcaataat taattcact cacgtttaa 
      ttaattgtg ttatcaaac gttggtaaa acaaccatt 
      cgttcgaaa tgcctcaag tcaaactcc acaaacatt 
      atgtcccca tcatccttt tatcaaaaa tcctcggaa 
      ttgcaccaa tcagctaca aaaacagta atgtaaact 
      attcaaaaa ttggaatcg tccaaactg ttgcctact 
      ttgtgattt tcataattt tgacaatcg tttttttcg 
      aaaaacgat cggctttga gtccgttcg aattgatcc 
      aaattcgca caattaact tcgttgcca cgtttcgaa 
      ctatagcca acatcatcc tcatggcgg tactgtttc 
      ttggtactt tcataggac gagcaaaat ttctggcga 
      ttttgaaac aaatgcgta atttggaac cggagtttc 
      tcaaactgg accaacttg gtgcaactg ttcgcaaca 
      atagacaaa ttcgaagaa tggtggtgt tcctgattg 
      gtgtctcgt tgagtttca atttgttcg gtgatcttc 
      tcacacttt ttataaaag aagaactgg acttggttt 
      ggagggaag tttgcactt ttgtggtac aagtctacc 
      ctcgactgg ttgtaaata ccatcaatt taacgtttt 
      tgactcttt tgatgattc tggaaggtc gctttagag 
      tcattaatg acggaatcc aagccctca ttaatgact 
      ctcgcaaag tgacacggt ttccaaccg aattgacct 
      tttgcaaca aaatcaaac atctggtca accttcaca 
      aaaacttgg tcaaagtcg ccaaagatc acaattcgc 
      tttattcct tgcatctgt tactttgga ttactgtga 
      cttttattc ttcttgtcg gtgactcct atattgtca 
      tgtatgtat tttttttcc acttatcag atgttcgct 
      atgttatgg ttgtgtact tgttgaaaa atgtggtcc 
      tgtatggtt tagaattgt ttattttgg cgaaaattg 
      tgaccgact tgtgtcaag aagcgaata gtaccaaaa 
      aattaattg tgaaaatta aaatcgaca tcgacaagg 
      aggatgaaa ggaatgagg tcatttcaa gtgctttga 
      aactgagtc agaatgagt tggaaataa cagcttgca 
      agtttttca gtattgata acgctctac tattttcag 
      taattcaaa aaaaattga ttgtgtgct tgttaaaaa 
      atgtggtcc tgtatgctt tggaatttt ttattttgg 
      cgaaaattg tgaccgact tgtgtcaag aagcaaata 
      gtaccaaaa aactaattg tgaaaatta gaattgaca 
      tcgacaagg aggatgaaa ggaatgagg tcatttcaa 
      gtgctttga aactgattc agaatgagt tggagataa 
      cagcttgca agtttttca gcattgata acgctctac 
      tattttcag taaaaaaag tcattgaat cacaaaaga 
      accagaaat cagtcgatt taacctttt gcgaaactt 
      tgaccgatt tgatccaaa ttcacaaaa ttttgacta 
      aaatcactg gaaaaatca acgcggttt ttgctttac 
      aacttttgt tgtggatcg gcttcaact ttctgttcc 
      tcctgatgg attcttaca ctacggctc acttaattt 
      ttttcgatt ggaagtcaa gtaaattgt atcctgttt 
      cgctcatgt tattgaatg ttttttcat actgttttt 
      acattttct acttgtcga gaagatgcg aacgggtct 
      gttttgaag caaaccgca ccgctttta tagtggctg 
      gcactagca tcgacttcc acaaagaag aagaacgaa 
      atttgatca tcacaggaa ttttaaatc tgatgaaaa 
      acaaactag aactaactg cttgtcgcc ttttcagta 
      ttgacaata ctttactat tttcagttt gtggtgccg 
      ctagctctt atttattta taatgctac agtttgatt 
      tggaggcat ttaagaatc taaaaacca gaaatagta 
      ctaattcca gtttctaat actagattt taagatatt 
      aatgtaata tataaaaaa aattaaaaa atgtttttt 
      tagaaaaaa taaaagttt ttaaataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY