Status
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Gene information
General information
TC033184
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013260
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11489172..11492079
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033184
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08359

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033184 RA
      MAFSSLYEE KCPFTRDYS LIKNELLNI DDYDKTCIE 
      TALHGVNQL VLGEWGNNT ACQIILITD GSTGLGAMS 
      LKESFASLN QRTAANPFP VPFSFPAKL HIVCIAPPN 
      DPNLAKSKS LYQRLVDLT GYDGSISIP DGPLNETTV 
      VNMFQKLAE DMYTSFKGT LKCGNLESK IILSPAPVP 
      YTKVTDFDY QTYNLSDLI EVCGFISVS DVGSPMAVS 
      RHLVLPASA NKDHLPLST EIDLTDDDA TDEGKVPSF 
      CVLLHGALK VENMAALVT LADNWFGFV YSWADSKKK 
      SNLMLTILT PGSDVVPWL GDLNNLGPI DSPQEHMGA 
      FPVRPQEKR SYSQNGVVW IRQAGLQSD IQKILRHAR 
      KLPEKTQQF YKELNRLRK AAISLGFLD LLNGLAYIF 
      EQECMQLPG SAHPDCALQ LQHAADVLR KTQNRDIKY 
      VVVPLQTNY NSS
    CDS sequence
    • >TC033184 RA
      atggcattt tcttcactt tatgaggaa aaatgtccc 
      tttaccaga gattacagc ctcattaaa aacgagctt 
      ctcaatatc gacgactac gacaaaaca tgcatcgaa 
      acggctctc catggcgtt aatcagttg gttttaggc 
      gaatgggga aataataca gcttgtcag ataatacta 
      attactgat ggaagtacc ggtttaggc gccatgtct 
      ctgaaagag tccttcgct agtttgaac caacgaacg 
      gctgcaaac ccctttcct gtaccattt tcattcccc 
      gccaaactc cacattgtc tgcatcgcg cctccgaat 
      gacccaaat ttggccaaa tcaaagtcg ctatatcag 
      cgattggtc gatttgacc ggctatgac ggatcaatt 
      tcaatcccc gatggtccc ttaaacgag accacggtg 
      gtcaacatg tttcaaaaa ttagccgaa gacatgtac 
      acctcattc aaaggcacc ctaaagtgt ggtaacctc 
      gagtctaaa ataatcctc tcaccggcc cctgtcccc 
      tacactaaa gtcaccgac ttcgactac caaacctac 
      aatttatca gaccttatc gaggtttgc ggcttcatt 
      tctgtaagc gatgtcggg tccccaatg gccgtaagc 
      cgccacttg gtcctccca gctagtgcc aacaaagac 
      catttgcca ttatccacc gaaatcgac ctaacagac 
      gacgatgca accgacgag ggcaaagtc ccctcgttc 
      tgtgtcctc ttgcacgga gccctgaag gtcgaaaac 
      atggccgcg ttggtgact ctggccgac aattggttc 
      ggtttcgtg tactcttgg gccgactcg aagaaaaaa 
      tctaatcta atgttgacc attctgacg ccgggatcg 
      gatgttgtc ccctggctg ggcgatttg aataatctg 
      gggcccatc gactcgccc caggaacac atgggggcg 
      ttcccggta cgtccgcag gaaaaaagg agctactcg 
      cagaatggg gtggtttgg atcaggcaa gccgggctc 
      cagagcgac atacagaag attctgagg catgcccgg 
      aaactgccc gaaaaaacc caacagttt tataaggag 
      ttgaatcga cttcggaag gccgcaatt tcgctcggc 
      ttcttggac cttttgaac ggtctggcg tacatcttc 
      gaacaggag tgcatgcaa cttccagga agcgcgcat 
      cctgactgt gcgctccag ctccaacac gcggccgat 
      gttttgcga aagacgcaa aatcgcgat attaaatat 
      gtcgttgtt cctttacaa acgaattac aacagttct 
      taa
    mRNA sequence
    • >TC033184 RA
      tgataaact agaaatact taaaacgcg attcaacgc 
      tcagttaat taatttcca gatggcatt ttcttcact 
      ttatgagga aaaatgtcc ctttaccag agattacag 
      cctcattaa aaacgagct tctcaatat cgacgacta 
      cgacaaaac atgcatcga aacggctct ccatggcgt 
      taatcagtt ggttttagg cgaatgggg aaataatac 
      agcttgtca gataatact aattactga tggaagtac 
      cggtttagg cgccatgtc tctgaaaga gtccttcgc 
      tagtttgaa ccaacgaac ggctgcaaa cccctttcc 
      tgtaccatt ttcattccc cgccaaact ccacattgt 
      ctgcatcgc gcctccgaa tgacccaaa tttggccaa 
      atcaaagtc gctatatca gcgattggt cgatttgac 
      cggctatga cggatcaat ttcaatccc cgatggtcc 
      cttaaacga gaccacggt ggtcaacat gtttcaaaa 
      attagccga agacatgta cacctcatt caaaggcac 
      cctaaagtg tggtaacct cgagtctaa aataatcct 
      ctcaccggc ccctgtccc ctacactaa agtcaccga 
      cttcgacta ccaaaccta caatttatc agaccttat 
      cgaggtttg cggcttcat ttctgtaag cgatgtcgg 
      gtccccaat ggccgtaag ccgccactt ggtcctccc 
      agctagtgc caacaaaga ccatttgcc attatccac 
      cgaaatcga cctaacaga cgacgatgc aaccgacga 
      gggcaaagt cccctcgtt ctgtgtcct cttgcacgg 
      agccctgaa ggtcgaaaa catggccgc gttggtgac 
      tctggccga caattggtt cggtttcgt gtactcttg 
      ggccgactc gaagaaaaa atctaatct aatgttgac 
      cattctgac gccgggatc ggatgttgt cccctggct 
      gggcgattt gaataatct ggggcccat cgactcgcc 
      ccaggaaca catgggggc gttcccggt acgtccgca 
      ggaaaaaag gagctactc gcagaatgg ggtggtttg 
      gatcaggca agccgggct ccagagcga catacagaa 
      gattctgag gcatgcccg gaaactgcc cgaaaaaac 
      ccaacagtt ttataagga gttgaatcg acttcggaa 
      ggccgcaat ttcgctcgg cttcttgga ccttttgaa 
      cggtctggc gtacatctt cgaacagga gtgcatgca 
      acttccagg aagcgcgca tcctgactg tgcgctcca 
      gctccaaca cgcggccga tgttttgcg aaagacgca 
      aaatcgcga tattaaata tgtcgttgt tcctttaca 
      aacgaatta caacagttc ttaattttt tatttcact 
      cttttattt tacaacaag taaaaacct tactcctga 
      ttaaatacg gaaatttcg gtaacaaca cgaggcata 
      aaacacttc ggagcgagc agccaaagt agtgtagca 
      tcgcaaggt aggtgatgt ttgtttcac ttaaataat 
      tgttataca gtggcggca ttttgaaac tattgactg 
      tgtttttcc caaaagtgt gcgaaagca cgtggatga 
      agtgcaaaa actgtcttt gaaacgtag tcaggaaat 
      agccaatcg tgagaatta ttccaaaaa tcaagagtt 
      tccgaattt cgcacattt gcttcgtta gcacatttt 
      cgatgtcgc tggtcgaag tttgcgtca gtttgattg 
      ttaggataa cgagggaca aaaagagta acaacaccg 
      gcagggcaa ttttaaaaa tcacaagcg agtgttgca 
      aaacttgtc cataataag agtctatga ccgcacaca 
      gcgtgtaat tttgcttga gtttgagcg tcgacgcca 
      gggtaaaat tcggaacac tgagcgaca ggctcacaa 
      tccacacga aaatgaccg ggaagacga tcgagacgt 
      cgtctttgc gtttgctgt aatcaatgg tcaccagcc 
      gatttgcgt tttcctgct tggtgacga gacactcat 
      tggagtcgt ctttgtcgc atgacgtga gcgggttct 
      tgttgcttt ttttcccgt gcgtttcgt gcaaattcc 
      agagggaaa tgacgtgaa aattaaagc agattatca 
      agtagattg ttagagtgt tcaccagga cctcggtcc 
      ctttgaaaa aggggcaaa attccgggg acaaatacc 
      agctttggt ggcgcagag tcaggattt cggtcgctc 
      ccacgtact gagcggcca agtcggcga atgaaattt 
      gcaggatta gggataaaa tgcctaaaa tcggttaat 
      taattgcag atttctaaa tcagtgtta tgagcaact 
      atgtgagaa atattaatt accgtttct cctgcttct 
      tattaatgc taaaatatg aagcacgtt tagaacacc 
      gccgcaggc ttgcagagc aaaataagc ggcgactat 
      ccattcctg ggcttgctc ttgagagat cgttccgtt 
      gtaactact tgtaaaatt atcaaaaac taaaactgg 
      cacactttc attttcttc tccatcgat attcatctt 
      atgagcgta ttaactcat gaaagacat gacgaagac 
      tggcatcta ttgcagggg agagcgcac gttgaatat 
      aatagttaa gtgtct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY