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Gene information
General information
TC033184
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013260 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11489172..11492079
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033184
    iBeetle screen iB_08359

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033184 RA
      MAFSSLYEE KCPFTRDYS LIKNELLNI DDYDKTCIE 
TALHGVNQL VLGEWGNNT ACQIILITD GSTGLGAMS 
LKESFASLN QRTAANPFP VPFSFPAKL HIVCIAPPN 
DPNLAKSKS LYQRLVDLT GYDGSISIP DGPLNETTV 
VNMFQKLAE DMYTSFKGT LKCGNLESK IILSPAPVP 
YTKVTDFDY QTYNLSDLI EVCGFISVS DVGSPMAVS 
RHLVLPASA NKDHLPLST EIDLTDDDA TDEGKVPSF 
CVLLHGALK VENMAALVT LADNWFGFV YSWADSKKK 
SNLMLTILT PGSDVVPWL GDLNNLGPI DSPQEHMGA 
FPVRPQEKR SYSQNGVVW IRQAGLQSD IQKILRHAR 
KLPEKTQQF YKELNRLRK AAISLGFLD LLNGLAYIF 
EQECMQLPG SAHPDCALQ LQHAADVLR KTQNRDIKY 
VVVPLQTNY NSS
    CDS sequence
    • >TC033184 RA
      atggcattt tcttcactt tatgaggaa aaatgtccc 
tttaccaga gattacagc ctcattaaa aacgagctt 
ctcaatatc gacgactac gacaaaaca tgcatcgaa 
acggctctc catggcgtt aatcagttg gttttaggc 
gaatgggga aataataca gcttgtcag ataatacta 
attactgat ggaagtacc ggtttaggc gccatgtct 
ctgaaagag tccttcgct agtttgaac caacgaacg 
gctgcaaac ccctttcct gtaccattt tcattcccc 
gccaaactc cacattgtc tgcatcgcg cctccgaat 
gacccaaat ttggccaaa tcaaagtcg ctatatcag 
cgattggtc gatttgacc ggctatgac ggatcaatt 
tcaatcccc gatggtccc ttaaacgag accacggtg 
gtcaacatg tttcaaaaa ttagccgaa gacatgtac 
acctcattc aaaggcacc ctaaagtgt ggtaacctc 
gagtctaaa ataatcctc tcaccggcc cctgtcccc 
tacactaaa gtcaccgac ttcgactac caaacctac 
aatttatca gaccttatc gaggtttgc ggcttcatt 
tctgtaagc gatgtcggg tccccaatg gccgtaagc 
cgccacttg gtcctccca gctagtgcc aacaaagac 
catttgcca ttatccacc gaaatcgac ctaacagac 
gacgatgca accgacgag ggcaaagtc ccctcgttc 
tgtgtcctc ttgcacgga gccctgaag gtcgaaaac 
atggccgcg ttggtgact ctggccgac aattggttc 
ggtttcgtg tactcttgg gccgactcg aagaaaaaa 
tctaatcta atgttgacc attctgacg ccgggatcg 
gatgttgtc ccctggctg ggcgatttg aataatctg 
gggcccatc gactcgccc caggaacac atgggggcg 
ttcccggta cgtccgcag gaaaaaagg agctactcg 
cagaatggg gtggtttgg atcaggcaa gccgggctc 
cagagcgac atacagaag attctgagg catgcccgg 
aaactgccc gaaaaaacc caacagttt tataaggag 
ttgaatcga cttcggaag gccgcaatt tcgctcggc 
ttcttggac cttttgaac ggtctggcg tacatcttc 
gaacaggag tgcatgcaa cttccagga agcgcgcat 
cctgactgt gcgctccag ctccaacac gcggccgat 
gttttgcga aagacgcaa aatcgcgat attaaatat 
gtcgttgtt cctttacaa acgaattac aacagttct 
taa
    mRNA sequence
    • >TC033184 RA
      tgataaact agaaatact taaaacgcg attcaacgc 
tcagttaat taatttcca gatggcatt ttcttcact 
ttatgagga aaaatgtcc ctttaccag agattacag 
cctcattaa aaacgagct tctcaatat cgacgacta 
cgacaaaac atgcatcga aacggctct ccatggcgt 
taatcagtt ggttttagg cgaatgggg aaataatac 
agcttgtca gataatact aattactga tggaagtac 
cggtttagg cgccatgtc tctgaaaga gtccttcgc 
tagtttgaa ccaacgaac ggctgcaaa cccctttcc 
tgtaccatt ttcattccc cgccaaact ccacattgt 
ctgcatcgc gcctccgaa tgacccaaa tttggccaa 
atcaaagtc gctatatca gcgattggt cgatttgac 
cggctatga cggatcaat ttcaatccc cgatggtcc 
cttaaacga gaccacggt ggtcaacat gtttcaaaa 
attagccga agacatgta cacctcatt caaaggcac 
cctaaagtg tggtaacct cgagtctaa aataatcct 
ctcaccggc ccctgtccc ctacactaa agtcaccga 
cttcgacta ccaaaccta caatttatc agaccttat 
cgaggtttg cggcttcat ttctgtaag cgatgtcgg 
gtccccaat ggccgtaag ccgccactt ggtcctccc 
agctagtgc caacaaaga ccatttgcc attatccac 
cgaaatcga cctaacaga cgacgatgc aaccgacga 
gggcaaagt cccctcgtt ctgtgtcct cttgcacgg 
agccctgaa ggtcgaaaa catggccgc gttggtgac 
tctggccga caattggtt cggtttcgt gtactcttg 
ggccgactc gaagaaaaa atctaatct aatgttgac 
cattctgac gccgggatc ggatgttgt cccctggct 
gggcgattt gaataatct ggggcccat cgactcgcc 
ccaggaaca catgggggc gttcccggt acgtccgca 
ggaaaaaag gagctactc gcagaatgg ggtggtttg 
gatcaggca agccgggct ccagagcga catacagaa 
gattctgag gcatgcccg gaaactgcc cgaaaaaac 
ccaacagtt ttataagga gttgaatcg acttcggaa 
ggccgcaat ttcgctcgg cttcttgga ccttttgaa 
cggtctggc gtacatctt cgaacagga gtgcatgca 
acttccagg aagcgcgca tcctgactg tgcgctcca 
gctccaaca cgcggccga tgttttgcg aaagacgca 
aaatcgcga tattaaata tgtcgttgt tcctttaca 
aacgaatta caacagttc ttaattttt tatttcact 
cttttattt tacaacaag taaaaacct tactcctga 
ttaaatacg gaaatttcg gtaacaaca cgaggcata 
aaacacttc ggagcgagc agccaaagt agtgtagca 
tcgcaaggt aggtgatgt ttgtttcac ttaaataat 
tgttataca gtggcggca ttttgaaac tattgactg 
tgtttttcc caaaagtgt gcgaaagca cgtggatga 
agtgcaaaa actgtcttt gaaacgtag tcaggaaat 
agccaatcg tgagaatta ttccaaaaa tcaagagtt 
tccgaattt cgcacattt gcttcgtta gcacatttt 
cgatgtcgc tggtcgaag tttgcgtca gtttgattg 
ttaggataa cgagggaca aaaagagta acaacaccg 
gcagggcaa ttttaaaaa tcacaagcg agtgttgca 
aaacttgtc cataataag agtctatga ccgcacaca 
gcgtgtaat tttgcttga gtttgagcg tcgacgcca 
gggtaaaat tcggaacac tgagcgaca ggctcacaa 
tccacacga aaatgaccg ggaagacga tcgagacgt 
cgtctttgc gtttgctgt aatcaatgg tcaccagcc 
gatttgcgt tttcctgct tggtgacga gacactcat 
tggagtcgt ctttgtcgc atgacgtga gcgggttct 
tgttgcttt ttttcccgt gcgtttcgt gcaaattcc 
agagggaaa tgacgtgaa aattaaagc agattatca 
agtagattg ttagagtgt tcaccagga cctcggtcc 
ctttgaaaa aggggcaaa attccgggg acaaatacc 
agctttggt ggcgcagag tcaggattt cggtcgctc 
ccacgtact gagcggcca agtcggcga atgaaattt 
gcaggatta gggataaaa tgcctaaaa tcggttaat 
taattgcag atttctaaa tcagtgtta tgagcaact 
atgtgagaa atattaatt accgtttct cctgcttct 
tattaatgc taaaatatg aagcacgtt tagaacacc 
gccgcaggc ttgcagagc aaaataagc ggcgactat 
ccattcctg ggcttgctc ttgagagat cgttccgtt 
gtaactact tgtaaaatt atcaaaaac taaaactgg 
cacactttc attttcttc tccatcgat attcatctt 
atgagcgta ttaactcat gaaagacat gacgaagac 
tggcatcta ttgcagggg agagcgcac gttgaatat 
aatagttaa gtgtct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY