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Gene information
General information
TC013304 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11278711..11283809
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013304
    iBeetle screen iB_08356

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013304 RA
      MSETKAQIS CFLFAALLL TTESHTYCT SSYGVETCS 
      FLPAAPGKT PPCALPGLT YCEHPEHYP GERIQYLIQ 
      KWRFDHSTI LINESKEDF TSYYYPPPK PKYGPPNTL 
      PAYYPEPIH IPKPNYVYQ QNRAGNTYI PPPYNSTQT 
      FGGFPQGQY VKYKYSNNI PPSVPREAY GPPSYLSPL 
      ALPQINPYA NKVWNRKDD KYGKALLIR KKRSSHLRK 
      LKSLVLPSN GTTLERTKR QNALTGQTL CTSRSQYIM 
      PRAALNNKG NWMYVVNMP ELDNRFSQL VKSETCASQ 
      TCSGLCGLP VGYTSRCEQ KYVQKRLVA LEGAGNELY 
      TDVFWFPSC CVCTISNG
    CDS sequence
    • >TC013304 RA
      atgagtgaa acaaaggcc caaatctcc tgttttctc 
      tttgctgct ttgctccta accaccgaa agtcacact 
      tactgcacc agctcctac ggggtggaa acgtgcagc 
      tttctgccc gcagccccc ggaaaaacg cccccgtgc 
      gccctcccg ggactcacc tactgcgag caccccgaa 
      cactacccc ggcgaaagg attcaatat ttgatccaa 
      aaatggaga ttcgatcat tcgactatt ttaataaat 
      gagtccaaa gaagacttc acctcgtat tactaccct 
      ccccctaag cccaaatac gggccccct aacaccctc 
      cccgcctac taccccgaa cccatccac atccctaag 
      cccaactac gtgtaccaa cagaacagg gcgggaaac 
      acctacata ccgccccct tacaactcc actcaaact 
      tttggcgga ttcccccaa ggccagtat gtgaaatac 
      aaatactcg aataatata ccaccaagt gttccaagg 
      gaggcttac ggccctcct agctactta tcacccttg 
      gcccttccc caaatcaac ccttacgcc aataaagta 
      tggaacagg aaagacgac aagtacgga aaagccctt 
      ctaatacga aaaaaacgc tccagtcat ttaagaaaa 
      ctgaaatct ctggttttg ccctcaaat ggtaccacc 
      cttgagcgc accaagcgc caaaacgcc ctcacaggg 
      caaaccctt tgcacttca cgatctcag tacatcatg 
      cctagggcg gctctgaac aacaagggc aactggatg 
      tacgttgtg aacatgcca gagctggac aataggttc 
      agccagctt gtcaaaagt gaaacttgc gcatcgcag 
      acgtgcagc gggctttgt ggcctccct gtgggctat 
      acgtccaga tgcgagcag aagtacgtg cagaagagg 
      ctagtggcc ttggagggt gctgggaat gaactctac 
      accgatgtg ttctggttt cctagttgt tgtgtttgt 
      accatttcg aacggatga
    mRNA sequence
    • >TC013304 RA
      cccattcac ctgtcactg tcagtaggt cgccccatt 
      gtccccacc tattactct aataagtag gttcttgta 
      tatgtgaga tacttgttg gtagcgcac atgtcgatg 
      aatattgac ccttcaaat gttcgcaaa ctcgcctgg 
      tagttaata catctcaag tgtctcaaa ccgcaactg 
      tgatgagtg aaacaaagg cccaaatct cctgttttc 
      tctttgctg ctttgctcc taaccaccg aaagtcaca 
      cttactgca ccagctcct acggggtgg aaacgtgca 
      gctttctgc ccgcagccc ccggaaaaa cgcccccgt 
      gcgccctcc cgggactca cctactgcg agcaccccg 
      aacactacc ccggcgaaa ggattcaat atttgatcc 
      aaaaatgga gattcgatc attcgacta ttttaataa 
      atgagtcca aagaagact tcacctcgt attactacc 
      ctcccccta agcccaaat acgggcccc ctaacaccc 
      tccccgcct actaccccg aacccatcc acatcccta 
      agcccaact acgtgtacc aacagaaca gggcgggaa 
      acacctaca taccgcccc cttacaact ccactcaaa 
      cttttggcg gattccccc aaggccagt atgtgaaat 
      acaaatact cgaataata taccaccaa gtgttccaa 
      gggaggctt acggccctc ctagctact tatcaccct 
      tggcccttc cccaaatca acccttacg ccaataaag 
      tatggaaca ggaaagacg acaagtacg gaaaagccc 
      ttctaatac gaaaaaaac gctccagtc atttaagaa 
      aactgaaat ctctggttt tgccctcaa atggtacca 
      cccttgagc gcaccaagc gccaaaacg ccctcacag 
      ggcaaaccc tttgcactt cacgatctc agtacatca 
      tgcctaggg cggctctga acaacaagg gcaactgga 
      tgtacgttg tgaacatgc cagagctgg acaataggt 
      tcagccagc ttgtcaaaa gtgaaactt gcgcatcgc 
      agacgtgca gcgggcttt gtggcctcc ctgtgggct 
      atacgtcca gatgcgagc agaagtacg tgcagaaga 
      ggctagtgg ccttggagg gtgctggga atgaactct 
      acaccgatg tgttctggt ttcctagtt gttgtgttt 
      gtaccattt cgaacggat gatttttgt atcttgaac 
      gtgtagtag cgtttttta attatagtc aaagaaatg 
      gtttgtgtt ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY