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Gene information
General information
TC014177 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2824096..2827428
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014177
    iBeetle screen iB_08354

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014177 RA
      MDTLNVFDF LDRIVKLIK EEHILNPQE ANKVIEYVD 
      PHDLKNKID LSLAAPCAN LEELEAVCR DIIKYSIKT 
      NSAKFHNQL YGGTDLYGL GATWLAESL NTNQHTFEV 
      APVFSLVEL ELLEEVKKL LGYTQGDGI FSPGGSISN 
      MYGMVLARY KKFPETKTK GLHGLPVLV AFTSEEGHY 
      SLQKSAQWL GLGTDNLVK IKTDNFGRM IAEELEKAI 
      ISRKAQGHV PFFVNATAG TTVVGAIDP LDKIADICE 
      RHQLWLHID ACYGGTLLL SKNFKERLE ASHRSDSFA 
      WNPHKMLGA PLQCSIFIT RHNNILHEC NSASAVYLF 
      QQDKFYDVS YDTGDKSIQ CGRKVDGFK LWVMWKARG 
      KAGFASLVD NAIDCANYF RSKISNLEG FRLVQDEFE 
      TTAVCFWYI PRSMRSGKE DAEWWKKMN NVAPAIKEK 
      LVKSGKLMI GYSPLVHRG FCNFFRMIT TCHPVPTYS 
      DMDYVIEQI KQIGEVM
    CDS sequence
    • >TC014177 RA
      atggataca ttaaacgtt tttgacttt ttggacaga 
      atagtaaaa ctgattaaa gaggaacac attctaaat 
      ccccaggaa gctaataaa gtcattgaa tatgtagat 
      ccccacgat ttgaagaac aaaattgat ttatccttg 
      gcggctccg tgcgcgaat ttggaagag cttgaagcg 
      gtttgcaga gatataata aaatacagc attaaaaca 
      aatagtgcg aaatttcat aaccagctc tacgggggc 
      acggatctg tacggtctt ggtgcaacc tggctcgct 
      gaatcactg aataccaat cagcacacg ttcgaagtc 
      gcccccgtg ttctcccta gtcgagtta gaactctta 
      gaggaagtt aaaaaattg ctcggctat acgcagggc 
      gatgggatt ttttcccca ggcggtagc atatcaaat 
      atgtacggg atggtcctc gcccgctac aaaaaattc 
      cccgagacc aaaacaaag ggcctccat ggcttacca 
      gtcctggtc gcctttacc tcggaagaa ggacattat 
      tctctccaa aagagcgcg cagtggctg ggtctaggc 
      accgataat ttggttaag attaaaacc gataatttc 
      ggacggatg attgccgag gagctcgaa aaggcaatc 
      atctcgagg aaggcgcag ggccacgtc ccgtttttt 
      gtcaacgct actgcaggg accacggtc gtgggcgcg 
      attgaccct ttagataag atagccgat atttgcgaa 
      aggcaccag ctttggctc cacatcgac gcttgttat 
      ggaggcact cttttactt tctaaaaat tttaaagaa 
      cggctggag gcatcgcac agatcggac tcgtttgct 
      tggaaccca cacaaaatg ttaggtgct ccattgcaa 
      tgctcaata tttataaca agacataac aatattctt 
      catgaatgt aattcagct tcagctgtc tatttattc 
      cagcaagat aagttttac gatgtctcg tacgatacg 
      ggggataag agcatacag tgcggccgc aaagttgac 
      ggtttcaaa ttatgggta atgtggaaa gctcgtggc 
      aaagcgggg ttcgcaagc cttgtggat aatgccatc 
      gactgtgct aattatttt aggtcaaaa atttcgaat 
      ttggagggt tttagactg gttcaggat gagttcgag 
      accacggct gtttgcttt tggtacatt cctcgtagc 
      atgcgctcc gggaaagaa gacgccgaa tggtggaaa 
      aaaatgaac aatgtggct cccgcaatt aaagaaaaa 
      ttagtgaag agtggcaaa ctgatgata ggttattcg 
      cctttggtt catcgaggt ttctgcaat ttttttcgc 
      atgattacc acttgtcat cccgttccg acttattcg 
      gacatggac tacgtcatc gagcaaata aaacaaatt 
      ggggaagtt atgtaa
    mRNA sequence
    • >TC014177 RA
      aacgtctga aaaatccat ttaatcggc aatcatttg 
      ttggatggt acttgccat ggatacatt aaacgtttt 
      tgacttttt ggacagaat agtaaaact gattaaaga 
      ggaacacat tctaaatcc ccaggaagc taataaagt 
      cattgaata tgtagatcc ccacgattt gaagaacaa 
      aattgattt atccttggc ggctccgtg cgcgaattt 
      ggaagagct tgaagcggt ttgcagaga tataataaa 
      atacagcat taaaacaaa tagtgcgaa atttcataa 
      ccagctcta cgggggcac ggatctgta cggtcttgg 
      tgcaacctg gctcgctga atcactgaa taccaatca 
      gcacacgtt cgaagtcgc ccccgtgtt ctccctagt 
      cgagttaga actcttaga ggaagttaa aaaattgct 
      cggctatac gcagggcga tgggatttt ttccccagg 
      cggtagcat atcaaatat gtacgggat ggtcctcgc 
      ccgctacaa aaaattccc cgagaccaa aacaaaggg 
      cctccatgg cttaccagt cctggtcgc ctttacctc 
      ggaagaagg acattattc tctccaaaa gagcgcgca 
      gtggctggg tctaggcac cgataattt ggttaagat 
      taaaaccga taatttcgg acggatgat tgccgagga 
      gctcgaaaa ggcaatcat ctcgaggaa ggcgcaggg 
      ccacgtccc gttttttgt caacgctac tgcagggac 
      cacggtcgt gggcgcgat tgacccttt agataagat 
      agccgatat ttgcgaaag gcaccagct ttggctcca 
      catcgacgc ttgttatgg aggcactct tttactttc 
      taaaaattt taaagaacg gctggaggc atcgcacag 
      atcggactc gtttgcttg gaacccaca caaaatgtt 
      aggtgctcc attgcaatg ctcaatatt tataacaag 
      acataacaa tattcttca tgaatgtaa ttcagcttc 
      agctgtcta tttattcca gcaagataa gttttacga 
      tgtctcgta cgatacggg ggataagag catacagtg 
      cggccgcaa agttgacgg tttcaaatt atgggtaat 
      gtggaaagc tcgtggcaa agcggggtt cgcaagcct 
      tgtggataa tgccatcga ctgtgctaa ttattttag 
      gtcaaaaat ttcgaattt ggagggttt tagactggt 
      tcaggatga gttcgagac cacggctgt ttgcttttg 
      gtacattcc tcgtagcat gcgctccgg gaaagaaga 
      cgccgaatg gtggaaaaa aatgaacaa tgtggctcc 
      cgcaattaa agaaaaatt agtgaagag tggcaaact 
      gatgatagg ttattcgcc tttggttca tcgaggttt 
      ctgcaattt ttttcgcat gattaccac ttgtcatcc 
      cgttccgac ttattcgga catggacta cgtcatcga 
      gcaaataaa acaaattgg ggaagttat gtaattttt 
      ctttaataa atacatgag tcgtaaaaa gtgtttaat 
      ttaatcggc agtgaagtg tcgctaatg agcctaaac 
      ctattaatg atacaatgc cgcagacgt aagagagca 
      catcttcgt agaagtgtg ggcacattt ccttcggcg 
      tacattggc tgcaataaa aaacgctat aactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY