Status
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Gene information
General information
TC014468 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10695989..10699389
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014468
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08345

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014468 RA
      MPHQYGLPG IAHAQSPPT PPPQHGAMY SRFSGAVVP 
      GGYRGEDRR LTREAMERY LRDRSDMVI VILHAKVAQ 
      KSYGNEKRF FCPPPCIYL FGDGWRLRQ EQMLREGES 
      EQASQLCAF IGIGNSDQD MQQLDLNNG KQYCAAKTL 
      YISDSDKRK HFMLSVKMF YGSGHDIGV FHSKRIKVI 
      SKPSKKKQS LKNADLCIA SGTKVALFN RLRSQTVST 
      RYLHVENGH FHASSTQWG AFTIHLLDD NESESEEFQ 
      VRDGYIHYG STVKLVCSV TGMALPRLI IRKVDKQQA 
      LLEADDPVS QLHKCAFYM KDTERMYLC LSQERIIQF 
      QATPCPKEP NKEMINDGA CWTIISTDK AEYQFYEGM 
      GPVRSPVTP VPIVHSLHL NGGGEVAML ELTGDNFSP 
      SLQVWFGDV EAETMYRCQ ESMLCVVPD ISQFRGEWL 
      WVRQPTQVP VSLVRNDGI IYATGLTFT YTPEPGPRP 
      QCLPADEIM RHGQRSAAA AQGQSQALT QEAAWNSHG 
      GMH
    CDS sequence
    • >TC014468 RA
      atgccccat cagtacgga ttgccgggc atagcccac 
      gctcagtcg cccccgacg ccgccgccg cagcacggg 
      gccatgtac tcgcggttc tcgggggcg gtggtgccc 
      gggggctac cgcggcgag gaccggcgg ctgacccgg 
      gaggccatg gagcggtat ctgcgggac cggtccgac 
      atggtcatc gtgattttg cacgccaag gtggcgcag 
      aagtcctac gggaatgag aagaggttt ttctgtccg 
      cccccttgc atttatctg ttcggggac ggctggagg 
      ctgcgccag gagcagatg ctgcgggag ggcgagtcc 
      gagcaggcc tcgcagtta tgcgctttc atagggatt 
      gggaactcg gaccaggac atgcagcag ctggatttg 
      aataatggg aagcagtat tgtgccgcg aaaactctc 
      tacatctcc gattcggac aagaggaaa cactttatg 
      ttgtccgtg aagatgttc tacgggtcg gggcacgac 
      atcggggtg tttcatagc aaaagaatc aaagttatc 
      tcgaagcct tcaaagaag aaacaatcg ttgaaaaac 
      gcggatttg tgtattgcg agtggtacc aaagtggcg 
      ctttttaac agacttaga tctcagact gtctccacg 
      cggtatctg cacgtggaa aatggacat tttcatgcc 
      agttccaca cagtggggt gctttcact atacacttg 
      ttagacgac aatgaatct gaaagtgag gagtttcaa 
      gtacgggat gggtacatt cattacggt tctacagtc 
      aaactggtg tgttctgtc accggaatg gccctcccg 
      agacttatc attcgaaag gtggacaag cagcaggcg 
      ctcctcgaa gccgacgac cccgtgtcc cagctgcac 
      aaatgcgcc ttttacatg aaagacacc gaacgcatg 
      tacctctgc ctctcccaa gagcgcatc atccagttc 
      caggccacc ccttgcccc aaagagccc aacaaggag 
      atgatcaac gacggcgcc tgctggacc atcatctcc 
      accgacaag gccgagtac cagttctac gagggcatg 
      ggccccgtg cgctccccc gtgaccccc gtgcccatc 
      gtgcacagc ctgcacttg aacggcggc ggcgaagtg 
      gcgatgctg gagctcacg ggcgacaac ttctcgccg 
      tccctgcag gtgtggttc ggggacgtg gaggccgag 
      accatgtac cggtgccag gagtccatg ctgtgcgtg 
      gtcccggac atctcgcag ttccggggc gagtggctg 
      tgggtgcgg cagccgacc caggtcccg gtctccttg 
      gtgcggaac gacgggatt atctatgcc acggggttg 
      acgttcacg tatacgccg gagccgggg ccccgaccg 
      cagtgtttg cctgcggac gagatcatg aggcatggg 
      cagaggagc gcggcggcg gcgcagggg cagagccag 
      gcgctgacg caggaggcg gcgtggaac tcgcacgga 
      gggatgcat tag
    mRNA sequence
    • >TC014468 RA
      tgagcgtta aaaaattcc aagaacgcg ttaattcgg 
      gcacttcct gtgaaaact cgccttttg aaagttggc 
      catcctgtc ggaaaatcc tctctccaa acaggtgtt 
      tttcgaatg agatacggg gggcaaagg agggggaag 
      catggcatt tgttctcac cgtgtgttg cggtagccg 
      tgcgaagag ttggctgtg agtgcatcg ataaaacaa 
      acgccagtg cgatgcggc ctacttgat taagtgttt 
      cgaaatgcc ccatcagta cggattgcc gggcatagc 
      ccacgctca gtcgccccc gacgccgcc gccgcagca 
      cggggccat gtactcgcg gttctcggg ggcggtggt 
      gcccggggg ctaccgcgg cgaggaccg gcggctgac 
      ccgggaggc catggagcg gtatctgcg ggaccggtc 
      cgacatggt catcgtgat tttgcacgc caaggtggc 
      gcagaagtc ctacgggaa tgagaagag gtttttctg 
      tccgccccc ttgcattta tctgttcgg ggacggctg 
      gaggctgcg ccaggagca gatgctgcg ggagggcga 
      gtccgagca ggcctcgca gttatgcgc tttcatagg 
      gattgggaa ctcggacca ggacatgca gcagctgga 
      tttgaataa tgggaagca gtattgtgc cgcgaaaac 
      tctctacat ctccgattc ggacaagag gaaacactt 
      tatgttgtc cgtgaagat gttctacgg gtcggggca 
      cgacatcgg ggtgtttca tagcaaaag aatcaaagt 
      tatctcgaa gccttcaaa gaagaaaca atcgttgaa 
      aaacgcgga tttgtgtat tgcgagtgg taccaaagt 
      ggcgctttt taacagact tagatctca gactgtctc 
      cacgcggta tctgcacgt ggaaaatgg acattttca 
      tgccagttc cacacagtg gggtgcttt cactataca 
      cttgttaga cgacaatga atctgaaag tgaggagtt 
      tcaagtacg ggatgggta cattcatta cggttctac 
      agtcaaact ggtgtgttc tgtcaccgg aatggccct 
      cccgagact tatcattcg aaaggtgga caagcagca 
      ggcgctcct cgaagccga cgaccccgt gtcccagct 
      gcacaaatg cgcctttta catgaaaga caccgaacg 
      catgtacct ctgcctctc ccaagagcg catcatcca 
      gttccaggc caccccttg ccccaaaga gcccaacaa 
      ggagatgat caacgacgg cgcctgctg gaccatcat 
      ctccaccga caaggccga gtaccagtt ctacgaggg 
      catgggccc cgtgcgctc ccccgtgac ccccgtgcc 
      catcgtgca cagcctgca cttgaacgg cggcggcga 
      agtggcgat gctggagct cacgggcga caacttctc 
      gccgtccct gcaggtgtg gttcgggga cgtggaggc 
      cgagaccat gtaccggtg ccaggagtc catgctgtg 
      cgtggtccc ggacatctc gcagttccg gggcgagtg 
      gctgtgggt gcggcagcc gacccaggt cccggtctc 
      cttggtgcg gaacgacgg gattatcta tgccacggg 
      gttgacgtt cacgtatac gccggagcc ggggccccg 
      accgcagtg tttgcctgc ggacgagat catgaggca 
      tgggcagag gagcgcggc ggcggcgca ggggcagag 
      ccaggcgct gacgcagga ggcggcgtg gaactcgca 
      cggagggat gcattaggg ggtgggtag gtagatgca 
      ataaagtta ggtagggtg tacaacgac cattgtgtt 
      ttttctcga gtggacact cagtattaa attttgaga 
      ttggaagtg gcacgagtc ttctgtatt atgatttat 
      tgtgatatg ttttttgag taggcggtc gtagagaag 
      caaaagttg gtatcaatt tttccttat tttttaatt 
      gatgcagtt gaccggcct aagacttgt aggatgtgt 
      ttctacagc ggtgtaaag agatactta gccttatga 
      acaaaccaa agttatgta agttttcta attgtcagt 
      cagaatgaa tagtccaac aatttttac ttagtatta 
      agtaaatta tgctcaatt tgccgatga ataataaat 
      aataggtaa taaaaaatc tcgacagga aaacatgac 
      aacaacgaa tttatggca aatgtaatg agtgtaaat 
      atctttatt agaattagt tttattcta cgtaaaata 
      atacataaa caaactgta catgctgca tattttgta 
      gaaataaaa actatttga ataa
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