Status
Please wait ...
Gene information
General information
TC033159
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014427
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10307537..10311865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033159
    iBeetle screen iB_08335

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033159 RA
      MSINVTVSG NPVRINRGK MVTSYDPVK AEFDKRRLM 
      RLEQVRQQS KDIAENVRN KLKKEKSKQ LSQIEKDGE 
      VRLKNWQAR KLLELQNQY QEAIEEIGL GHKEAARVA 
      AEEEAFFED EEVRERVAE ERGREAVQR LQIERNEES 
      LKRAVPVQR KKLTRDEEN SRSNAVVKK VKQKQSPFK 
      KKKTQVFDS DSENRVPLL EGLGGDSDW NSDLGGTRR 
      VTGARKVAT EVQTSPTIF EIPTSDKAA QTNIHESAF 
      PSTSEFPYD TRISERIKR RAILKPETD YCNFAEKEV 
      PRVIGLCPC CLCHGKHLH GVEPVTIDD RPDVRATSA 
      ITVRDEGKS EKGQAETHP QNLDDVSES TVPQKTNAT 
      SKEDLSLRQ SRRVPQRPE QVTKPKPDI PTKPKVTLK 
      STKKRPENV ASGSGPQKV QFYDHPNRY TKFYVPKTS 
      HVEKIDDEN IIISPNVLS EEEMYEKMR QRDREAHLR 
      GQVALEKER IQREYRDLM KKLPVLQKQ ERIAEIGLD 
      KEKYHMSGD RLEELEKRR QNRLDNAYE ELLVSQKPA 
      LVTLPRKND DRSENHSLN LAWDVDFEP KSSTNHNRT 
      EQLSHLLTT LKSQKSHLL KEIENTLSK ESSSQFDEV 
      KSKSTDYPR RKRSRDDRP ISPTTSDVI PEEETTTVK 
      TKPGRKSTT GAPSPRKKR KTSRKVLVK QNSSTQTSP 
      KSGRSVATS PHVNTPVED SCKCSKKDS SEEVCEVVI 
      KIKDGEKPE VVVQPPFSE KSVKVVTEV EEGKDVERP 
      ASWREELTR TASSSSTSY LEPPQSGRS DSILKKIGD 
      MSRTMDSTV SSGASGKQL DPRLLVYIK KLLNMSRAS 
      IDDLTISAS DVSTPSHSV VETPSNNPL LKLLNVMKY 
      YNLDVADLQ KQLSYETDD SGRHYNTSE SIAVTTSTS 
      EGDKSVQFK SCTEVDTSS KVEGRKIQD YANMAASCT 
      KKISSLTAM IEKLRAEKK KMMSSGSSG SDKDLTLTS 
      YLQLPDKSP KQDEIDAKL LDIDYNYAE KLKQLSREE 
      LQVEKQKVE VREPTDQEL LTRLKSLLQ DSNSKNKDK 
      RAVESTKKF LQKIDDTCV TLINEKKLF EDKQEDRTV 
      GQVSEKTSV SLKDDKTPQ SFPLLLDIP KLPRLEAPS 
      LEKKRKKRP PPTKTLRPI PEPVVPHEL STIIEADSQ 
      LSAKAESPV KPKGRPGSS GSKSGDVPD ILTEVVKDK 
      SSTNSSDLS EMETMETML MSIGMEWAI PTLKKTREA 
      LALTSSSSG SKESGELSL REFLSKISS SSDVMSASD 
      SQGMSLIHD DNRMTSTPI AGYGEKKDG QPIFLTDSD 
      LSSVKENSG RAKDVEQRF FELQDHSGK KE
    CDS sequence
    • >TC033159 RA
      atgtccatc aacgtgact gtctccggg aaccctgtg 
      cgtataaat cgcggcaaa atggtgact tcttacgac 
      cccgtgaag gccgaattc gacaaacgc cgtttgatg 
      cgtcttgaa caagtccgc cagcagtcc aaagacatt 
      gctgagaat gtgcgtaat aaactgaag aaggagaaa 
      agcaagcaa ttgtcgcaa attgagaaa gacggggag 
      gtcaggctg aaaaactgg caagcgcgg aaattgcta 
      gaattgcaa aatcagtat caagaggcg attgaggag 
      atcgggttg gggcacaag gaggcggcg agggttgcc 
      gccgaagaa gaggcgttt tttgaagac gaggaggtc 
      agggagagg gttgcagag gagcggggg cgggaagcg 
      gttcaaagg ttgcaaatt gagcgcaat gaggagagt 
      ttgaaaagg gctgtgcct gttcagagg aagaaattg 
      acacgagat gaggaaaat agtcggtca aatgctgtt 
      gtaaaaaaa gtaaaacaa aagcaaagt cccttcaag 
      aagaaaaag acacaagtt tttgattca gatagtgag 
      aatcgggtg ccgctgctg gaggggttg gggggcgat 
      tctgactgg aattctgat ctggggggc acgaggagg 
      gtcaccggt gcgcggaaa gtggccacg gaggtgcaa 
      acaagtccc acaattttt gaaattccg acaagtgat 
      aaagccgca caaacgaat attcacgaa agtgctttt 
      ccaagcaca tccgaattt ccttacgac actcgaatt 
      tccgaacga ataaaacgc cgagcgatc cttaaaccg 
      gaaacggat tattgcaat tttgccgaa aaggaagtt 
      ccccgagta attggtttg tgtccgtgc tgtttatgt 
      cacgggaaa cacctccat ggtgttgaa ccggtaaca 
      attgacgat cgacctgac gttcgggcc acatcggca 
      attaccgtg agagatgag ggaaaatcg gaaaagggc 
      caagcggag acgcaccct caaaacctg gacgatgta 
      tctgagagt acagtccct caaaaaaca aatgctacg 
      agcaaagag gatttaagt ttgaggcaa agcaggcgt 
      gtgcctcaa aggccggaa caagtgact aagcctaaa 
      ccagacatt ccgacaaaa ccgaaagtc acattgaaa 
      tcgaccaaa aagcggccc gagaatgtg gcaagtggt 
      tcaggccct caaaaagtg caattttac gaccatccg 
      aaccgttac acaaagttc tacgtcccg aaaacatca 
      cacgtagaa aaaatcgac gacgaaaat attataatt 
      tcgccgaat gtcttaagc gaggaagaa atgtacgaa 
      aagatgcgc cagcgagac cgtgaggcg cacctccga 
      ggccaagtg gctttggaa aaagagcga atccaaagg 
      gagtatcgc gacttgatg aaaaaactc ccagtttta 
      caaaaacaa gagcgcatc gcggagatc ggcctcgat 
      aaagagaag taccacatg tcaggggat cgattagaa 
      gagttggaa aagaggaga caaaacaga cttgataac 
      gcctatgag gaattacta gtgagtcaa aagccggct 
      cttgtgaca cttccgcga aaaaatgac gatcggagt 
      gaaaatcat agtttaaat ctagcctgg gatgtcgat 
      ttcgagccc aaaagttca accaatcac aaccgtact 
      gaacaactg agtcactta ttaacgact ctgaagtcg 
      caaaaaagt cacttactc aaagaaatc gaaaatact 
      ctatcgaaa gaaagctcg agtcagttc gacgaagtc 
      aagtcgaag tcgaccgat tacccccga cggaaacga 
      agtcgggac gaccgcccg atttccccc accaccagc 
      gatgtgatc cctgaagag gaaaccaca acggtcaag 
      acgaaaccg ggccgcaaa agcaccact ggtgccccc 
      agtccccgc aaaaagcgc aaaacttcg cgaaaagtc 
      ctcgtgaag caaaacagc agcacccaa accagcccc 
      aagtcgggc cgaagtgtg gccacctcg ccccatgtg 
      aacaccccg gttgaagat tcgtgcaaa tgctccaag 
      aaagacagt tcggaggaa gtctgcgag gtggtgatt 
      aaaatcaaa gacggggaa aaaccggaa gttgtagtc 
      cagccgcct ttttcggag aagagtgtt aaagtggtt 
      acggaagtc gaggaggga aaagacgtt gaaaggccg 
      gcgtcgtgg cgcgaggag ttgaccagg actgcgtcc 
      agttccagc acttcgtac ttagagccc ccacagtcg 
      gggcgcagt gactcaatt ttgaaaaaa atcggggat 
      atgtcacgg actatggat agtacggtg tcaagcgga 
      gcgagtggc aaacagctc gatccccga cttttggtt 
      tacattaaa aaattgctg aatatgtcg agggctagt 
      attgacgat ttgacgatc agtgcaagt gacgtgtcc 
      acacccagc cacagtgtg gtcgaaacg ccgtccaac 
      aatccgctt ttgaaactc ttaaatgtt atgaaatac 
      tacaatttg gacgttgct gacttgcaa aagcaattg 
      agttacgag actgacgac agtggtcgg cattacaac 
      actagtgag agtattgct gtaacgaca agcacctcc 
      gagggtgac aagtcagta cagtttaag tcgtgtaca 
      gaggtggac acaagtagt aaagtcgaa gggaggaaa 
      atacaggac tatgccaat atggcggca tcttgtacg 
      aaaaaaatc tcaagcttg acggcaatg attgaaaaa 
      ttgcgtgcc gagaagaag aaaatgatg agttcggga 
      agtagtggc agtgataag gacttaacc ctcacttca 
      tatttgcaa ttaccggac aagagcccg aaacaagac 
      gagattgac gctaaactg ctagatatc gactataac 
      tatgccgaa aaattaaaa cagttgagt cgagaggaa 
      ctacaagtt gagaaacag aaagtcgaa gtgagggaa 
      cccacagat caggagctt ctgacacga ctgaaaagc 
      ctgttgcag gatagtaat agcaaaaat aaagacaaa 
      cgtgccgta gaaagcacc aagaaattt ttgcagaaa 
      atcgacgac acgtgtgtg acactaatc aacgaaaaa 
      aagttattt gaagacaag caagaggac aggactgtc 
      ggtcaagtg tccgaaaaa acctcagtc agtttaaaa 
      gacgacaaa acccctcaa tcatttccc ctacttttg 
      gacatccca aaactcccc cgtctcgag gcaccgtca 
      ctcgagaaa aagcgaaaa aagcgcccc ccgcctact 
      aaaaccctg cgccccatt cccgagcct gtcgttccg 
      cacgaactg tcgacgata atcgaagcc gatagtcag 
      ctgtcggcc aaagccgaa agccccgtc aaacccaag 
      gggagaccc ggttctagt ggttccaag tccggtgac 
      gtccctgat attttaacc gaggtggtc aaagacaag 
      tcgtcgact aattcgagc gacttgtcc gaaatggag 
      acaatggag accatgttg atgagtatc ggaatggag 
      tgggcgatt cccacgtta aagaaaacg cgcgaagcg 
      ttagcgttg acgtcaagc tccagcggg tccaaggag 
      agtggggaa ttgagtttg agggagttt ttgagtaag 
      atttcaagc agttcggat gtaatgtcg gcgagtgac 
      tcacaaggg atgtcgctg attcatgac gataatagg 
      atgacgtcg acgccgatt gcagggtac ggggagaaa 
      aaagacgga cagccgatt tttttgacg gattcggac 
      cttagttcg gttaaggag aattcagga agggccaag 
      gatgtggaa caaaggttt ttcgaattg caggatcac 
      tcgggaaag aaggaatga
    mRNA sequence
    • >TC033159 RA
      gtcgcatca aaacaaacc gacttgaca gctgacgct 
      gacgttccc atcatttga atctaaatt tgtttattt 
      ggctttttg gtccagttt ttagcctta aaattaatt 
      ttggccgac ttatcacgg ttatggact cctcaagcg 
      ctctttgag gttatgtcc cccaaccgt cgaaatgtc 
      catcaacgt gactgtctc cgggaaccc tgtgcgtat 
      aaatcgcgg caaaatggt gacttctta cgaccccgt 
      gaaggccga attcgacaa acgccgttt gatgcgtct 
      tgaacaagt ccgccagca gtccaaaga cattgctga 
      gaatgtgcg taataaact gaagaagga gaaaagcaa 
      gcaattgtc gcaaattga gaaagacgg ggaggtcag 
      gctgaaaaa ctggcaagc gcggaaatt gctagaatt 
      gcaaaatca gtatcaaga ggcgattga ggagatcgg 
      gttggggca caaggaggc ggcgagggt tgccgccga 
      agaagaggc gttttttga agacgagga ggtcaggga 
      gagggttgc agaggagcg ggggcggga agcggttca 
      aaggttgca aattgagcg caatgagga gagtttgaa 
      aagggctgt gcctgttca gaggaagaa attgacacg 
      agatgagga aaatagtcg gtcaaatgc tgttgtaaa 
      aaaagtaaa acaaaagca aagtccctt caagaagaa 
      aaagacaca agtttttga ttcagatag tgagaatcg 
      ggtgccgct gctggaggg gttgggggg cgattctga 
      ctggaattc tgatctggg gggcacgag gagggtcac 
      cggtgcgcg gaaagtggc cacggaggt gcaaacaag 
      tcccacaat ttttgaaat tccgacaag tgataaagc 
      cgcacaaac gaatattca cgaaagtgc ttttccaag 
      cacatccga atttcctta cgacactcg aatttccga 
      acgaataaa acgccgagc gatccttaa accggaaac 
      ggattattg caattttgc cgaaaagga agttccccg 
      agtaattgg tttgtgtcc gtgctgttt atgtcacgg 
      gaaacacct ccatggtgt tgaaccggt aacaattga 
      cgatcgacc tgacgttcg ggccacatc ggcaattac 
      cgtgagaga tgagggaaa atcggaaaa gggccaagc 
      ggagacgca ccctcaaaa cctggacga tgtatctga 
      gagtacagt ccctcaaaa aacaaatgc tacgagcaa 
      agaggattt aagtttgag gcaaagcag gcgtgtgcc 
      tcaaaggcc ggaacaagt gactaagcc taaaccaga 
      cattccgac aaaaccgaa agtcacatt gaaatcgac 
      caaaaagcg gcccgagaa tgtggcaag tggttcagg 
      ccctcaaaa agtgcaatt ttacgacca tccgaaccg 
      ttacacaaa gttctacgt cccgaaaac atcacacgt 
      agaaaaaat cgacgacga aaatattat aatttcgcc 
      gaatgtctt aagcgagga agaaatgta cgaaaagat 
      gcgccagcg agaccgtga ggcgcacct ccgaggcca 
      agtggcttt ggaaaaaga gcgaatcca aagggagta 
      tcgcgactt gatgaaaaa actcccagt tttacaaaa 
      acaagagcg catcgcgga gatcggcct cgataaaga 
      gaagtacca catgtcagg ggatcgatt agaagagtt 
      ggaaaagag gagacaaaa cagacttga taacgccta 
      tgaggaatt actagtgag tcaaaagcc ggctcttgt 
      gacacttcc gcgaaaaaa tgacgatcg gagtgaaaa 
      tcatagttt aaatctagc ctgggatgt cgatttcga 
      gcccaaaag ttcaaccaa tcacaaccg tactgaaca 
      actgagtca cttattaac gactctgaa gtcgcaaaa 
      aagtcactt actcaaaga aatcgaaaa tactctatc 
      gaaagaaag ctcgagtca gttcgacga agtcaagtc 
      gaagtcgac cgattaccc ccgacggaa acgaagtcg 
      ggacgaccg cccgatttc ccccaccac cagcgatgt 
      gatccctga agaggaaac cacaacggt caagacgaa 
      accgggccg caaaagcac cactggtgc ccccagtcc 
      ccgcaaaaa gcgcaaaac ttcgcgaaa agtcctcgt 
      gaagcaaaa cagcagcac ccaaaccag ccccaagtc 
      gggccgaag tgtggccac ctcgcccca tgtgaacac 
      cccggttga agattcgtg caaatgctc caagaaaga 
      cagttcgga ggaagtctg cgaggtggt gattaaaat 
      caaagacgg ggaaaaacc ggaagttgt agtccagcc 
      gcctttttc ggagaagag tgttaaagt ggttacgga 
      agtcgagga gggaaaaga cgttgaaag gccggcgtc 
      gtggcgcga ggagttgac caggactgc gtccagttc 
      cagcacttc gtacttaga gcccccaca gtcggggcg 
      cagtgactc aattttgaa aaaaatcgg ggatatgtc 
      acggactat ggatagtac ggtgtcaag cggagcgag 
      tggcaaaca gctcgatcc ccgactttt ggtttacat 
      taaaaaatt gctgaatat gtcgagggc tagtattga 
      cgatttgac gatcagtgc aagtgacgt gtccacacc 
      cagccacag tgtggtcga aacgccgtc caacaatcc 
      gcttttgaa actcttaaa tgttatgaa atactacaa 
      tttggacgt tgctgactt gcaaaagca attgagtta 
      cgagactga cgacagtgg tcggcatta caacactag 
      tgagagtat tgctgtaac gacaagcac ctccgaggg 
      tgacaagtc agtacagtt taagtcgtg tacagaggt 
      ggacacaag tagtaaagt cgaagggag gaaaataca 
      ggactatgc caatatggc ggcatcttg tacgaaaaa 
      aatctcaag cttgacggc aatgattga aaaattgcg 
      tgccgagaa gaagaaaat gatgagttc gggaagtag 
      tggcagtga taaggactt aaccctcac ttcatattt 
      gcaattacc ggacaagag cccgaaaca agacgagat 
      tgacgctaa actgctaga tatcgacta taactatgc 
      cgaaaaatt aaaacagtt gagtcgaga ggaactaca 
      agttgagaa acagaaagt cgaagtgag ggaacccac 
      agatcagga gcttctgac acgactgaa aagcctgtt 
      gcaggatag taatagcaa aaataaaga caaacgtgc 
      cgtagaaag caccaagaa atttttgca gaaaatcga 
      cgacacgtg tgtgacact aatcaacga aaaaaagtt 
      atttgaaga caagcaaga ggacaggac tgtcggtca 
      agtgtccga aaaaacctc agtcagttt aaaagacga 
      caaaacccc tcaatcatt tcccctact tttggacat 
      cccaaaact cccccgtct cgaggcacc gtcactcga 
      gaaaaagcg aaaaaagcg ccccccgcc tactaaaac 
      cctgcgccc cattcccga gcctgtcgt tccgcacga 
      actgtcgac gataatcga agccgatag tcagctgtc 
      ggccaaagc cgaaagccc cgtcaaacc caaggggag 
      acccggttc tagtggttc caagtccgg tgacgtccc 
      tgatatttt aaccgaggt ggtcaaaga caagtcgtc 
      gactaattc gagcgactt gtccgaaat ggagacaat 
      ggagaccat gttgatgag tatcggaat ggagtgggc 
      gattcccac gttaaagaa aacgcgcga agcgttagc 
      gttgacgtc aagctccag cgggtccaa ggagagtgg 
      ggaattgag tttgaggga gtttttgag taagatttc 
      aagcagttc ggatgtaat gtcggcgag tgactcaca 
      agggatgtc gctgattca tgacgataa taggatgac 
      gtcgacgcc gattgcagg gtacgggga gaaaaaaga 
      cggacagcc gattttttt gacggattc ggaccttag 
      ttcggttaa ggagaattc aggaagggc caaggatgt 
      ggaacaaag gtttttcga attgcagga tcactcggg 
      aaagaagga atgaaatag gatggggga ttatcgtgg 
      atgtaatta tttctctat ttgtaaatt tagtttttt 
      aataaaggt gtttttaaa at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY