| General information | |||
| TC033159
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 10307537..10311865 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033159 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08335 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033159 RA
MSINVTVSG NPVRINRGK MVTSYDPVK AEFDKRRLM RLEQVRQQS KDIAENVRN KLKKEKSKQ LSQIEKDGE VRLKNWQAR KLLELQNQY QEAIEEIGL GHKEAARVA AEEEAFFED EEVRERVAE ERGREAVQR LQIERNEES LKRAVPVQR KKLTRDEEN SRSNAVVKK VKQKQSPFK KKKTQVFDS DSENRVPLL EGLGGDSDW NSDLGGTRR VTGARKVAT EVQTSPTIF EIPTSDKAA QTNIHESAF PSTSEFPYD TRISERIKR RAILKPETD YCNFAEKEV PRVIGLCPC CLCHGKHLH GVEPVTIDD RPDVRATSA ITVRDEGKS EKGQAETHP QNLDDVSES TVPQKTNAT SKEDLSLRQ SRRVPQRPE QVTKPKPDI PTKPKVTLK STKKRPENV ASGSGPQKV QFYDHPNRY TKFYVPKTS HVEKIDDEN IIISPNVLS EEEMYEKMR QRDREAHLR GQVALEKER IQREYRDLM KKLPVLQKQ ERIAEIGLD KEKYHMSGD RLEELEKRR QNRLDNAYE ELLVSQKPA LVTLPRKND DRSENHSLN LAWDVDFEP KSSTNHNRT EQLSHLLTT LKSQKSHLL KEIENTLSK ESSSQFDEV KSKSTDYPR RKRSRDDRP ISPTTSDVI PEEETTTVK TKPGRKSTT GAPSPRKKR KTSRKVLVK QNSSTQTSP KSGRSVATS PHVNTPVED SCKCSKKDS SEEVCEVVI KIKDGEKPE VVVQPPFSE KSVKVVTEV EEGKDVERP ASWREELTR TASSSSTSY LEPPQSGRS DSILKKIGD MSRTMDSTV SSGASGKQL DPRLLVYIK KLLNMSRAS IDDLTISAS DVSTPSHSV VETPSNNPL LKLLNVMKY YNLDVADLQ KQLSYETDD SGRHYNTSE SIAVTTSTS EGDKSVQFK SCTEVDTSS KVEGRKIQD YANMAASCT KKISSLTAM IEKLRAEKK KMMSSGSSG SDKDLTLTS YLQLPDKSP KQDEIDAKL LDIDYNYAE KLKQLSREE LQVEKQKVE VREPTDQEL LTRLKSLLQ DSNSKNKDK RAVESTKKF LQKIDDTCV TLINEKKLF EDKQEDRTV GQVSEKTSV SLKDDKTPQ SFPLLLDIP KLPRLEAPS LEKKRKKRP PPTKTLRPI PEPVVPHEL STIIEADSQ LSAKAESPV KPKGRPGSS GSKSGDVPD ILTEVVKDK SSTNSSDLS EMETMETML MSIGMEWAI PTLKKTREA LALTSSSSG SKESGELSL REFLSKISS SSDVMSASD SQGMSLIHD DNRMTSTPI AGYGEKKDG QPIFLTDSD LSSVKENSG RAKDVEQRF FELQDHSGK KE
- >TC033159 RA
atgtccatc aacgtgact gtctccggg aaccctgtg cgtataaat cgcggcaaa atggtgact tcttacgac cccgtgaag gccgaattc gacaaacgc cgtttgatg cgtcttgaa caagtccgc cagcagtcc aaagacatt gctgagaat gtgcgtaat aaactgaag aaggagaaa agcaagcaa ttgtcgcaa attgagaaa gacggggag gtcaggctg aaaaactgg caagcgcgg aaattgcta gaattgcaa aatcagtat caagaggcg attgaggag atcgggttg gggcacaag gaggcggcg agggttgcc gccgaagaa gaggcgttt tttgaagac gaggaggtc agggagagg gttgcagag gagcggggg cgggaagcg gttcaaagg ttgcaaatt gagcgcaat gaggagagt ttgaaaagg gctgtgcct gttcagagg aagaaattg acacgagat gaggaaaat agtcggtca aatgctgtt gtaaaaaaa gtaaaacaa aagcaaagt cccttcaag aagaaaaag acacaagtt tttgattca gatagtgag aatcgggtg ccgctgctg gaggggttg gggggcgat tctgactgg aattctgat ctggggggc acgaggagg gtcaccggt gcgcggaaa gtggccacg gaggtgcaa acaagtccc acaattttt gaaattccg acaagtgat aaagccgca caaacgaat attcacgaa agtgctttt ccaagcaca tccgaattt ccttacgac actcgaatt tccgaacga ataaaacgc cgagcgatc cttaaaccg gaaacggat tattgcaat tttgccgaa aaggaagtt ccccgagta attggtttg tgtccgtgc tgtttatgt cacgggaaa cacctccat ggtgttgaa ccggtaaca attgacgat cgacctgac gttcgggcc acatcggca attaccgtg agagatgag ggaaaatcg gaaaagggc caagcggag acgcaccct caaaacctg gacgatgta tctgagagt acagtccct caaaaaaca aatgctacg agcaaagag gatttaagt ttgaggcaa agcaggcgt gtgcctcaa aggccggaa caagtgact aagcctaaa ccagacatt ccgacaaaa ccgaaagtc acattgaaa tcgaccaaa aagcggccc gagaatgtg gcaagtggt tcaggccct caaaaagtg caattttac gaccatccg aaccgttac acaaagttc tacgtcccg aaaacatca cacgtagaa aaaatcgac gacgaaaat attataatt tcgccgaat gtcttaagc gaggaagaa atgtacgaa aagatgcgc cagcgagac cgtgaggcg cacctccga ggccaagtg gctttggaa aaagagcga atccaaagg gagtatcgc gacttgatg aaaaaactc ccagtttta caaaaacaa gagcgcatc gcggagatc ggcctcgat aaagagaag taccacatg tcaggggat cgattagaa gagttggaa aagaggaga caaaacaga cttgataac gcctatgag gaattacta gtgagtcaa aagccggct cttgtgaca cttccgcga aaaaatgac gatcggagt gaaaatcat agtttaaat ctagcctgg gatgtcgat ttcgagccc aaaagttca accaatcac aaccgtact gaacaactg agtcactta ttaacgact ctgaagtcg caaaaaagt cacttactc aaagaaatc gaaaatact ctatcgaaa gaaagctcg agtcagttc gacgaagtc aagtcgaag tcgaccgat tacccccga cggaaacga agtcgggac gaccgcccg atttccccc accaccagc gatgtgatc cctgaagag gaaaccaca acggtcaag acgaaaccg ggccgcaaa agcaccact ggtgccccc agtccccgc aaaaagcgc aaaacttcg cgaaaagtc ctcgtgaag caaaacagc agcacccaa accagcccc aagtcgggc cgaagtgtg gccacctcg ccccatgtg aacaccccg gttgaagat tcgtgcaaa tgctccaag aaagacagt tcggaggaa gtctgcgag gtggtgatt aaaatcaaa gacggggaa aaaccggaa gttgtagtc cagccgcct ttttcggag aagagtgtt aaagtggtt acggaagtc gaggaggga aaagacgtt gaaaggccg gcgtcgtgg cgcgaggag ttgaccagg actgcgtcc agttccagc acttcgtac ttagagccc ccacagtcg gggcgcagt gactcaatt ttgaaaaaa atcggggat atgtcacgg actatggat agtacggtg tcaagcgga gcgagtggc aaacagctc gatccccga cttttggtt tacattaaa aaattgctg aatatgtcg agggctagt attgacgat ttgacgatc agtgcaagt gacgtgtcc acacccagc cacagtgtg gtcgaaacg ccgtccaac aatccgctt ttgaaactc ttaaatgtt atgaaatac tacaatttg gacgttgct gacttgcaa aagcaattg agttacgag actgacgac agtggtcgg cattacaac actagtgag agtattgct gtaacgaca agcacctcc gagggtgac aagtcagta cagtttaag tcgtgtaca gaggtggac acaagtagt aaagtcgaa gggaggaaa atacaggac tatgccaat atggcggca tcttgtacg aaaaaaatc tcaagcttg acggcaatg attgaaaaa ttgcgtgcc gagaagaag aaaatgatg agttcggga agtagtggc agtgataag gacttaacc ctcacttca tatttgcaa ttaccggac aagagcccg aaacaagac gagattgac gctaaactg ctagatatc gactataac tatgccgaa aaattaaaa cagttgagt cgagaggaa ctacaagtt gagaaacag aaagtcgaa gtgagggaa cccacagat caggagctt ctgacacga ctgaaaagc ctgttgcag gatagtaat agcaaaaat aaagacaaa cgtgccgta gaaagcacc aagaaattt ttgcagaaa atcgacgac acgtgtgtg acactaatc aacgaaaaa aagttattt gaagacaag caagaggac aggactgtc ggtcaagtg tccgaaaaa acctcagtc agtttaaaa gacgacaaa acccctcaa tcatttccc ctacttttg gacatccca aaactcccc cgtctcgag gcaccgtca ctcgagaaa aagcgaaaa aagcgcccc ccgcctact aaaaccctg cgccccatt cccgagcct gtcgttccg cacgaactg tcgacgata atcgaagcc gatagtcag ctgtcggcc aaagccgaa agccccgtc aaacccaag gggagaccc ggttctagt ggttccaag tccggtgac gtccctgat attttaacc gaggtggtc aaagacaag tcgtcgact aattcgagc gacttgtcc gaaatggag acaatggag accatgttg atgagtatc ggaatggag tgggcgatt cccacgtta aagaaaacg cgcgaagcg ttagcgttg acgtcaagc tccagcggg tccaaggag agtggggaa ttgagtttg agggagttt ttgagtaag atttcaagc agttcggat gtaatgtcg gcgagtgac tcacaaggg atgtcgctg attcatgac gataatagg atgacgtcg acgccgatt gcagggtac ggggagaaa aaagacgga cagccgatt tttttgacg gattcggac cttagttcg gttaaggag aattcagga agggccaag gatgtggaa caaaggttt ttcgaattg caggatcac tcgggaaag aaggaatga
- >TC033159 RA
gtcgcatca aaacaaacc gacttgaca gctgacgct gacgttccc atcatttga atctaaatt tgtttattt ggctttttg gtccagttt ttagcctta aaattaatt ttggccgac ttatcacgg ttatggact cctcaagcg ctctttgag gttatgtcc cccaaccgt cgaaatgtc catcaacgt gactgtctc cgggaaccc tgtgcgtat aaatcgcgg caaaatggt gacttctta cgaccccgt gaaggccga attcgacaa acgccgttt gatgcgtct tgaacaagt ccgccagca gtccaaaga cattgctga gaatgtgcg taataaact gaagaagga gaaaagcaa gcaattgtc gcaaattga gaaagacgg ggaggtcag gctgaaaaa ctggcaagc gcggaaatt gctagaatt gcaaaatca gtatcaaga ggcgattga ggagatcgg gttggggca caaggaggc ggcgagggt tgccgccga agaagaggc gttttttga agacgagga ggtcaggga gagggttgc agaggagcg ggggcggga agcggttca aaggttgca aattgagcg caatgagga gagtttgaa aagggctgt gcctgttca gaggaagaa attgacacg agatgagga aaatagtcg gtcaaatgc tgttgtaaa aaaagtaaa acaaaagca aagtccctt caagaagaa aaagacaca agtttttga ttcagatag tgagaatcg ggtgccgct gctggaggg gttgggggg cgattctga ctggaattc tgatctggg gggcacgag gagggtcac cggtgcgcg gaaagtggc cacggaggt gcaaacaag tcccacaat ttttgaaat tccgacaag tgataaagc cgcacaaac gaatattca cgaaagtgc ttttccaag cacatccga atttcctta cgacactcg aatttccga acgaataaa acgccgagc gatccttaa accggaaac ggattattg caattttgc cgaaaagga agttccccg agtaattgg tttgtgtcc gtgctgttt atgtcacgg gaaacacct ccatggtgt tgaaccggt aacaattga cgatcgacc tgacgttcg ggccacatc ggcaattac cgtgagaga tgagggaaa atcggaaaa gggccaagc ggagacgca ccctcaaaa cctggacga tgtatctga gagtacagt ccctcaaaa aacaaatgc tacgagcaa agaggattt aagtttgag gcaaagcag gcgtgtgcc tcaaaggcc ggaacaagt gactaagcc taaaccaga cattccgac aaaaccgaa agtcacatt gaaatcgac caaaaagcg gcccgagaa tgtggcaag tggttcagg ccctcaaaa agtgcaatt ttacgacca tccgaaccg ttacacaaa gttctacgt cccgaaaac atcacacgt agaaaaaat cgacgacga aaatattat aatttcgcc gaatgtctt aagcgagga agaaatgta cgaaaagat gcgccagcg agaccgtga ggcgcacct ccgaggcca agtggcttt ggaaaaaga gcgaatcca aagggagta tcgcgactt gatgaaaaa actcccagt tttacaaaa acaagagcg catcgcgga gatcggcct cgataaaga gaagtacca catgtcagg ggatcgatt agaagagtt ggaaaagag gagacaaaa cagacttga taacgccta tgaggaatt actagtgag tcaaaagcc ggctcttgt gacacttcc gcgaaaaaa tgacgatcg gagtgaaaa tcatagttt aaatctagc ctgggatgt cgatttcga gcccaaaag ttcaaccaa tcacaaccg tactgaaca actgagtca cttattaac gactctgaa gtcgcaaaa aagtcactt actcaaaga aatcgaaaa tactctatc gaaagaaag ctcgagtca gttcgacga agtcaagtc gaagtcgac cgattaccc ccgacggaa acgaagtcg ggacgaccg cccgatttc ccccaccac cagcgatgt gatccctga agaggaaac cacaacggt caagacgaa accgggccg caaaagcac cactggtgc ccccagtcc ccgcaaaaa gcgcaaaac ttcgcgaaa agtcctcgt gaagcaaaa cagcagcac ccaaaccag ccccaagtc gggccgaag tgtggccac ctcgcccca tgtgaacac cccggttga agattcgtg caaatgctc caagaaaga cagttcgga ggaagtctg cgaggtggt gattaaaat caaagacgg ggaaaaacc ggaagttgt agtccagcc gcctttttc ggagaagag tgttaaagt ggttacgga agtcgagga gggaaaaga cgttgaaag gccggcgtc gtggcgcga ggagttgac caggactgc gtccagttc cagcacttc gtacttaga gcccccaca gtcggggcg cagtgactc aattttgaa aaaaatcgg ggatatgtc acggactat ggatagtac ggtgtcaag cggagcgag tggcaaaca gctcgatcc ccgactttt ggtttacat taaaaaatt gctgaatat gtcgagggc tagtattga cgatttgac gatcagtgc aagtgacgt gtccacacc cagccacag tgtggtcga aacgccgtc caacaatcc gcttttgaa actcttaaa tgttatgaa atactacaa tttggacgt tgctgactt gcaaaagca attgagtta cgagactga cgacagtgg tcggcatta caacactag tgagagtat tgctgtaac gacaagcac ctccgaggg tgacaagtc agtacagtt taagtcgtg tacagaggt ggacacaag tagtaaagt cgaagggag gaaaataca ggactatgc caatatggc ggcatcttg tacgaaaaa aatctcaag cttgacggc aatgattga aaaattgcg tgccgagaa gaagaaaat gatgagttc gggaagtag tggcagtga taaggactt aaccctcac ttcatattt gcaattacc ggacaagag cccgaaaca agacgagat tgacgctaa actgctaga tatcgacta taactatgc cgaaaaatt aaaacagtt gagtcgaga ggaactaca agttgagaa acagaaagt cgaagtgag ggaacccac agatcagga gcttctgac acgactgaa aagcctgtt gcaggatag taatagcaa aaataaaga caaacgtgc cgtagaaag caccaagaa atttttgca gaaaatcga cgacacgtg tgtgacact aatcaacga aaaaaagtt atttgaaga caagcaaga ggacaggac tgtcggtca agtgtccga aaaaacctc agtcagttt aaaagacga caaaacccc tcaatcatt tcccctact tttggacat cccaaaact cccccgtct cgaggcacc gtcactcga gaaaaagcg aaaaaagcg ccccccgcc tactaaaac cctgcgccc cattcccga gcctgtcgt tccgcacga actgtcgac gataatcga agccgatag tcagctgtc ggccaaagc cgaaagccc cgtcaaacc caaggggag acccggttc tagtggttc caagtccgg tgacgtccc tgatatttt aaccgaggt ggtcaaaga caagtcgtc gactaattc gagcgactt gtccgaaat ggagacaat ggagaccat gttgatgag tatcggaat ggagtgggc gattcccac gttaaagaa aacgcgcga agcgttagc gttgacgtc aagctccag cgggtccaa ggagagtgg ggaattgag tttgaggga gtttttgag taagatttc aagcagttc ggatgtaat gtcggcgag tgactcaca agggatgtc gctgattca tgacgataa taggatgac gtcgacgcc gattgcagg gtacgggga gaaaaaaga cggacagcc gattttttt gacggattc ggaccttag ttcggttaa ggagaattc aggaagggc caaggatgt ggaacaaag gtttttcga attgcagga tcactcggg aaagaagga atgaaatag gatggggga ttatcgtgg atgtaatta tttctctat ttgtaaatt tagtttttt aataaaggt gtttttaaa at
