| General information | |||
| TC014424 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 10273227..10286370 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014424 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08334 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014424 RA
MEEGPDLSH LTPEERAII ESVMVRQKQ EEEREQEIM RRKADEVHI LEETIRARN EQHKKAGIE LDATCHICL KTKFADGVG HLCNYCSIR CCARCGGKV TLRSNKVVW VCILCRKKQ ELLSKTGQW MLGGTNADS ELPSLQQAP SDKRPKLER AHSAAEKEN QPLQRSGSV LRRQYSEQE TRTPSCERY NQQYNEDDP RFYQGELEG LMRTHPHLV PRRYPDHEP EPLPMDIPR MPSASANKK HKRSGSTKK HQHNPQPQL QRSFSSSEE DLKSTPEYG SDDRDSEKG DNGDWPPLS HYIVAPQHR PLQDVTADN RCFTERRKK TVRFDQHEN WDRQGSQDS HRDSGIDTS STFTSSEDS TRGDLPKHP LSWQVSANG CRMIGHMIL RRGSRETSG SSANILGLK VVGGKILEN GLRGAIIEK VKKGSIADI EGQLRPGDE VIEWNGRPL QGKTYQEVA DIIAESKHE ANVELIVSR SMGSRRAAQ ATWRQSVAH RGQSGQWQQ EVEGYERPH VLVTSPGSP ERPRPSPAL APSVGGRIQ IKLGFDPST LQLFVTIVC AAGLTPRSP HQPRSPYCK LFLLPDRSE KSKRRTRTL AGTEPMWNQ TFVYSGLRR ADLRQRALE ITVWDYVRY GANNFLGEA IVELWPLDE QPTWRTLGP HEEVALTPS EHLSPPSTG SRFSDSDTP SECEIDGGR ERRGADGAS VSSVGSSSS PPNERRSRR DQETMQAYG AQGYRRSMA GQRSHSAAP CDSPSVHMS RSRSKSPRH TVTDHRSLS PPDVRIAVA DYQPSYVVP RFQSRSATA TPTGSPKKR QLPQVPQAL ARALQERVA QDLDQDRAG RHRMRYMHT YRSTGSGWE RRYSGLSDS DLANHSRNV RRPVSPDRD RDPLGLVDF DSDMESVGS VTSSAFSTQ SERIRSNKP ASTEYGGGS DRYGYTTSH RSVPRNRER QDLRDPIEP IDQHNHHHH HHHHRESSI KRGQFTRSL SNTEPPPDE KADGSLSDT AVGQIHGLE VPQKEMTRK ESQRERERE RADRQTGQF VGLSKKSNS TSQLSATGR KRRMGFGRR GKTSFTVHR SEEVIPGDV RLSKQGSSA SSDGEGSAD GDRWSPSLR LAGESGQLA DFIDGLGPG QLVGRQVLG APALGEIQL SLCYQKGFL EVEVIRARG LQARPGSKV LPAPYVKVY LVNGKKCIA KAKTSTARR TLDPLYQQQ LAFRENFQG CILQVTVWG DYGRIEGKK VFMGVAQIM LDDLNLSNI VIGWYKLFG TTSLVSGPP SLGLSRRSS MASLDSLKL
- >TC014424 RA
atggaggaa gggccggat ctgtctcac ctcactccc gaggagcgg gccatcatc gagtccgtc atggtccgg cagaagcag gaggaggag cgcgagcag gagatcatg aggagaaag gccgacgag gtgcacatc ttggaggag acgatacgg gcccgtaac gaacaacac aagaaggcc ggcatcgag ctggacgcc acctgccac atctgccta aagacgaaa ttcgccgac ggcgtcggc cacctgtgc aactactgc tcgatccgg tgctgtgcc cgctgcggg ggcaaagtc accctccgg tcgaataag gtggtgtgg gtttgcatc ctctgcaga aaaaagcaa gaactgttg agtaagact ggccaatgg atgcttggc ggcaccaac gccgacagc gagctgccg agcctccag caggcgccc tccgacaaa cggcccaag ctcgagcgt gcccattcg gccgccgag aaggagaac caacctctt cagcgctcc ggcagcgtg cttcgccga caatactcg gaacaagag acccgcacg ccctcgtgc gagcgctac aaccagcag tataacgag gacgatccg cgtttctac caaggagag ctcgaaggc ctcatgcgg acgcacccc catctcgtc ccgagacgc tatccggac cacgagccc gagcccctg cccatggac atcccccgg atgccctcc gcaagtgcc aacaagaaa cacaagcgc agcggctcg acgaagaaa caccagcac aacccccag ccgcagctg cagcggtcc ttctccagc tcggaggag gacctcaag tccaccccc gagtacggc agcgacgac cgcgacagc gagaaagga gacaacggc gactggcct cccctaagc cactatatc gtcgcccca caacacaga cctctgcag gacgtcacc gccgacaat cggtgcttc accgaacgc agaaagaag acggtcagg tttgaccaa cacgagaac tgggacaga cagggatcg caggactct catagagat tcggggatt gatacctcc tcgaccttc acctccagc gaggattcc actcgcgga gacctgccc aagcacccg ttatcttgg caagtgtcc gccaacgga tgtcgaatg atcggtcat atgatactc cgacgagga tcgagggaa acttccggt tctagtgcg aatatctta ggactgaaa gtggtcgga ggtaaaatc ctcgagaac ggtctcaga ggtgcgatt atcgaaaaa gtcaaaaaa ggaagtatc gccgacatt gaaggccaa ctaaggccg ggtgatgaa gtgattgag tggaatggt cgccctctc caaggaaaa acatatcaa gaagtagcc gatatcata gctgagagc aaacacgaa gcgaatgtt gagttgatc gtttcccga agtatggga agcagacga gccgcccaa gcgacctgg aggcagtct gtggcccat agaggtcag tctggtcaa tggcagcag gaagtggag ggttacgaa agaccgcac gtcctcgta acgtcgccg gggagtccc gaacgaccc agacccagt cccgccttg gctcccagc gtcgggggc agaatacaa atcaagctc ggtttcgac ccctccact ttgcagttg ttcgtcacg atcgtgtgc gcggcgggc ttgacgccg aggtcgccc caccagcca cgcagtcct tactgcaag ctcttcctc ctcccggac cgctccgag aagtccaag cggcggact cgcaccctc gcaggaacg gaaccgatg tggaaccaa accttcgtc tactcgggc cttcgtcga gcggacctg cggcagcgc gccttggag atcaccgtc tgggactat gtccgctac ggcgccaac aatttttta ggcgaggcc atcgtcgaa ttgtggcct ttggacgaa cagcccact tggcggacc cttgggccc cacgaggag gtggccttg acgcccagc gagcacttg tcgcccccg agcaccggc tcgcgcttc tccgactcg gacacgccg tccgagtgc gagatcgac ggcgggagg gagcgtcgc ggcgccgac ggagccagc gtcagcagc gtcggcagc tcatcctcg ccccctaac gagcggaga tccaggaga gaccaagag acgatgcaa gcctacggc gcgcagggg tatcgaaga agcatggcg ggccagagg tcgcactcg gccgcgccg tgcgactcc cccagcgtg cacatgtcg cgctccagg tccaagtcg ccccgacac accgtcacc gaccaccgt tccctgtcg cctcccgac gtccgtatc gccgtagcc gactaccag ccgtcatac gtcgtgcct cgtttccag tcccgttcg gcgactgcg acccccacc ggatcgccg aagaaacgc caactgccc caagtgccc caagcgctg gcgcgcgcc ctccaagag cgcgtggcc caagacctg gaccaggac agggccggg aggcaccgc atgaggtac atgcacacg tacaggagc accggctcg ggatgggaa cgcagatat agtggactg agtgatagt gatttggcg aaccatagc cggaatgtc cgaaggccg gtgtcgccg gacagggac agggacccc ctaggcttg gtggacttt gatagtgac atggaatcg gtcgggtct gtgactagt agtgcgttt tccacgcag tcggaacga atcaggagc aacaaacct gccagtact gaatatggt ggaggtagc gatcgatat gggtacacg acgagtcat cgcagtgtc cccagaaac agagaaaga caagacttg cgggaccct atcgagccc atcgaccaa cacaaccat caccaccac caccatcac cacagggaa agtagcata aaaaggggg cagtttacg cggagcttg tcgaacacg gagccccca ccagacgaa aaagcagat ggcagtctg agtgatact gctgtgggc cagatccac ggtttggaa gtccctcag aaggagatg acgcggaag gagagccag cgggagcgg gaaagggag cgggcggat cgtcagacg ggccagttc gtaggactc agtaaaaag agtaactcg acaagtcag ttgtcggca acaggtagg aaacggagg atgggcttc ggtcgaagg ggcaagacc tcgttcact gttcatcga tcggaggag gttattccc ggagatgtg aggctgtcc aagcaaggc tcctcggct tccagtgac ggagaaggg tcggccgac ggcgacagg tggtcaccc tctttaaga ctggcgggc gagagtggg caactggcc gacttcatc gacggtttg ggaccagga caactagta ggacgacaa gtgctggga gcaccggct ttaggcgaa atccaactg agtctctgc taccaaaag gggttcctc gaagttgag gtgatacga gcgcgggga ctacaagcc agacccgga tccaaagtt ttaccagca ccctacgtc aaggtctat ctagtcaac ggcaaaaaa tgcatagcc aaggccaaa acctcgacg gctcgacga actttagat ccgctctac cagcaacag ttagctttc agagagaat ttccaaggc tgtatactc caggtgacc gtctggggt gactacggg cgcatcgag ggcaagaaa gtgttcatg ggcgtggcc caaatcatg ctcgatgac ctcaacttg agcaacatc gtcatcgga tggtacaaa ctcttcggc accacatcc ctggttagt ggacctcct tcgctcggt ctctctcga cgcagctcc atggctagc ttagattcc ctcaagttg taa
- >TC014424 RA
tattattta ttaaaggta catgaatgg gtaattgca aactgtgat atttggccg gacgacggc tgagacaat cgaaaaaaa tcgcgaaca agtcggtgc gaaggtccg tgtgcgcta gggccgcgt cgcgagtgc cccgagagg ccgtcgcca gggagccgc ctgtggaca tagccgcat gttgttgca tttcggtgc cgctctgag atggacgac gtgaagccg cggcgcgac cgcgacctc tccaacggg tagtcggtg gcatgtccg gaaagggct agcatggag gaagggccg gatctgtct cacctcact cccgaggag cgggccatc atcgagtcc gtcatggtc cggcagaag caggaggag gagcgcgag caggagatc atgaggaga aaggccgac gaggtgcac atcttggag gagacgata cgggcccgt aacgaacaa cacaagaag gccggcatc gagctggac gccacctgc cacatctgc ctaaagacg aaattcgcc gacggcgtc ggccacctg tgcaactac tgctcgatc cggtgctgt gcccgctgc gggggcaaa gtcaccctc cggtcgaat aaggtggtg tgggtttgc atcctctgc agaaaaaag caagaactg ttgagtaag actggccaa tggatgctt ggcggcacc aacgccgac agcgagctg ccgagcctc cagcaggcg ccctccgac aaacggccc aagctcgag cgtgcccat tcggccgcc gagaaggag aaccaacct cttcagcgc tccggcagc gtgcttcgc cgacaatac tcggaacaa gagacccgc acgccctcg tgcgagcgc tacaaccag cagtataac gaggacgat ccgcgtttc taccaagga gagctcgaa ggcctcatg cggacgcac ccccatctc gtcccgaga cgctatccg gaccacgag cccgagccc ctgcccatg gacatcccc cggatgccc tccgcaagt gccaacaag aaacacaag cgcagcggc tcgacgaag aaacaccag cacaacccc cagccgcag ctgcagcgg tccttctcc agctcggag gaggacctc aagtccacc cccgagtac ggcagcgac gaccgcgac agcgagaaa ggagacaac ggcgactgg cctccccta agccactat atcgtcgcc ccacaacac agacctctg caggacgtc accgccgac aatcggtgc ttcaccgaa cgcagaaag aagacggtc aggtttgac caacacgag aactgggac agacaggga tcgcaggac tctcataga gattcgggg attgatacc tcctcgacc ttcacctcc agcgaggat tccactcgc ggagacctg cccaagcac ccgttatct tggcaagtg tccgccaac ggatgtcga atgatcggt catatgata ctccgacga ggatcgagg gaaacttcc ggttctagt gcgaatatc ttaggactg aaagtggtc ggaggtaaa atcctcgag aacggtctc agaggtgcg attatcgaa aaagtcaaa aaaggaagt atcgccgac attgaaggc caactaagg ccgggtgat gaagtgatt gagtggaat ggtcgccct ctccaagga aaaacatat caagaagta gccgatatc atagctgag agcaaacac gaagcgaat gttgagttg atcgtttcc cgaagtatg ggaagcaga cgagccgcc caagcgacc tggaggcag tctgtggcc catagaggt cagtctggt caatggcag caggaagtg gagggttac gaaagaccg cacgtcctc gtaacgtcg ccggggagt cccgaacga cccagaccc agtcccgcc ttggctccc agcgtcggg ggcagaata caaatcaag ctcggtttc gacccctcc actttgcag ttgttcgtc acgatcgtg tgcgcggcg ggcttgacg ccgaggtcg ccccaccag ccacgcagt ccttactgc aagctcttc ctcctcccg gaccgctcc gagaagtcc aagcggcgg actcgcacc ctcgcagga acggaaccg atgtggaac caaaccttc gtctactcg ggccttcgt cgagcggac ctgcggcag cgcgccttg gagatcacc gtctgggac tatgtccgc tacggcgcc aacaatttt ttaggcgag gccatcgtc gaattgtgg cctttggac gaacagccc acttggcgg acccttggg ccccacgag gaggtggcc ttgacgccc agcgagcac ttgtcgccc ccgagcacc ggctcgcgc ttctccgac tcggacacg ccgtccgag tgcgagatc gacggcggg agggagcgt cgcggcgcc gacggagcc agcgtcagc agcgtcggc agctcatcc tcgccccct aacgagcgg agatccagg agagaccaa gagacgatg caagcctac ggcgcgcag gggtatcga agaagcatg gcgggccag aggtcgcac tcggccgcg ccgtgcgac tcccccagc gtgcacatg tcgcgctcc aggtccaag tcgccccga cacaccgtc accgaccac cgttccctg tcgcctccc gacgtccgt atcgccgta gccgactac cagccgtca tacgtcgtg cctcgtttc cagtcccgt tcggcgact gcgaccccc accggatcg ccgaagaaa cgccaactg ccccaagtg ccccaagcg ctggcgcgc gccctccaa gagcgcgtg gcccaagac ctggaccag gacagggcc gggaggcac cgcatgagg tacatgcac acgtacagg agcaccggc tcgggatgg gaacgcaga tatagtgga ctgagtgat agtgatttg gcgaaccat agccggaat gtccgaagg ccggtgtcg ccggacagg gacagggac cccctaggc ttggtggac tttgatagt gacatggaa tcggtcggg tctgtgact agtagtgcg ttttccacg cagtcggaa cgaatcagg agcaacaaa cctgccagt actgaatat ggtggaggt agcgatcga tatgggtac acgacgagt catcgcagt gtccccaga aacagagaa agacaagac ttgcgggac cctatcgag cccatcgac caacacaac catcaccac caccaccat caccacagg gaaagtagc ataaaaagg gggcagttt acgcggagc ttgtcgaac acggagccc ccaccagac gaaaaagca gatggcagt ctgagtgat actgctgtg ggccagatc cacggtttg gaagtccct cagaaggag atgacgcgg aaggagagc cagcgggag cgggaaagg gagcgggcg gatcgtcag acgggccag ttcgtagga ctcagtaaa aagagtaac tcgacaagt cagttgtcg gcaacaggt aggaaacgg aggatgggc ttcggtcga aggggcaag acctcgttc actgttcat cgatcggag gaggttatt cccggagat gtgaggctg tccaagcaa ggctcctcg gcttccagt gacggagaa gggtcggcc gacggcgac aggtggtca ccctcttta agactggcg ggcgagagt gggcaactg gccgacttc atcgacggt ttgggacca ggacaacta gtaggacga caagtgctg ggagcaccg gctttaggc gaaatccaa ctgagtctc tgctaccaa aaggggttc ctcgaagtt gaggtgata cgagcgcgg ggactacaa gccagaccc ggatccaaa gttttacca gcaccctac gtcaaggtc tatctagtc aacggcaaa aaatgcata gccaaggcc aaaacctcg acggctcga cgaacttta gatccgctc taccagcaa cagttagct ttcagagag aatttccaa ggctgtata ctccaggtg accgtctgg ggtgactac gggcgcatc gagggcaag aaagtgttc atgggcgtg gcccaaatc atgctcgat gacctcaac ttgagcaac atcgtcatc ggatggtac aaactcttc ggcaccaca tccctggtt agtggacct ccttcgctc ggtctctct cgacgcagc tccatggct agcttagat tccctcaag ttgtaacac tgaccattc cattgtttc acacacact gactcgcgc ctgctattt ctatttgtc acattatca ctccctcat tatcatcat agtgtcacc gatcctcgt agtctacgc aactcttcc acttcgctt aggtacgcc taggtttga gtgaacacg ctgtatatt tgcttagaa ctgttagat catcctttc tgcccccgg gtttggtgc aaatgtttt ctgatgatt tttttgcag tgagggcga attccggcg tggttggag gcgacgtga cggcgagag aaagaagac agtcgccgg aatattcgc cggtagtgc gacaaactt tgcaaaaac atatacatg agactatat tccgattta atgcatttc cggcgaata ttaatgaaa aatattgta cttatcaga gtattacgg tatggtgcg aaaattatt agctgacat ttagaagca gattaaaat agatacata tagtgtaaa tgaagtgaa aaaagatat ataagtgta gatatcacg cacacatat aaatatagt atcacataa aatatcata tctaaaata tttgttata tgacgtctg gggcggccc aaaaaacta ccacctaga ttcatatgc gcgtgatta caacgatcc acttaaaaa tacaacaaa tgacttgta tttttctgg gacgtcctg ttcaaacaa caaaaatgc ttcattgga attgataaa ttgcttttt tacttgaaa ctaagtaat gacatgaaa ttaggtcgt gttaatgaa ctatgtaaa ttatgataa tgacattgt agaactgta tatgaacgt ttaattgta ccatataat ttattcttg attgttgaa ttgcatgtg atataactt gaagatttt atgttggct gtttgaatt tcccgcttc gataagata atctccgtt ctgaagcac aggtgtcgc cacgtttcc gtttcgttg tgtataaaa catataaca tcgaaaact gtaatgtaa tcgaagttt cccgcgatt cgatttcag aatattctc tcatttttg taacgtttg taatgaatt tttagaaag ggggcgtgt cgctgatat tcgactgtt tttccgcag ccgactgct acgacaccg cggtgtttt tactaccga ttcggcaaa cagtgccaa atcgggtat taaaaatgg ccggttttt taaattgtt tagtaaaac aatccgaat aatgcttct aagtcatag taaaagctt taatctgct tgaaaccca aaatatatc atacgtaac aacaagaat aagattttc cgaaacaac tataaccat aattatgat tgtatataa ttttcgaag aattgggtt caactaaaa tgtaataca ctgcataca gttcagatg gttgattta aatattata aaagatatc aatagttag tttttctac gctgttctt taataaaat aagacaccc acttcatgg atcatgtac tcccatttg ttccaaggt ccgaactgt attgtacct tttatcatt ccgattaaa tcattatca ataaccgag tgtagatat gcgccttac atactgttc gcagcagcc agtccggcc ctgcgtcac ttttcaata aaggcccgt gtaaattgc atcaattgg actaaaatg cggcaagta tggcgtcgg actggctcg ttgcacttt tgtatctgt accggacct tgaagggtt aagagacga cgaacaaag ctgtcattt gcgcttgag acacttcgg ctactgaac tgtatttta gtgtacggt cctttgaca atttgcgca tatcactgg aagtgtcag aagcgtcca ctgtatgtc cgtcttgat atgttcact cttgactga tgtatccat tttatccaa gcccatggg caagacaat aaatgaatg atctatcc
