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Gene information
General information
TC014424 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10273227..10286370
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014424
    iBeetle screen iB_08334

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014424 RA
      MEEGPDLSH LTPEERAII ESVMVRQKQ EEEREQEIM 
      RRKADEVHI LEETIRARN EQHKKAGIE LDATCHICL 
      KTKFADGVG HLCNYCSIR CCARCGGKV TLRSNKVVW 
      VCILCRKKQ ELLSKTGQW MLGGTNADS ELPSLQQAP 
      SDKRPKLER AHSAAEKEN QPLQRSGSV LRRQYSEQE 
      TRTPSCERY NQQYNEDDP RFYQGELEG LMRTHPHLV 
      PRRYPDHEP EPLPMDIPR MPSASANKK HKRSGSTKK 
      HQHNPQPQL QRSFSSSEE DLKSTPEYG SDDRDSEKG 
      DNGDWPPLS HYIVAPQHR PLQDVTADN RCFTERRKK 
      TVRFDQHEN WDRQGSQDS HRDSGIDTS STFTSSEDS 
      TRGDLPKHP LSWQVSANG CRMIGHMIL RRGSRETSG 
      SSANILGLK VVGGKILEN GLRGAIIEK VKKGSIADI 
      EGQLRPGDE VIEWNGRPL QGKTYQEVA DIIAESKHE 
      ANVELIVSR SMGSRRAAQ ATWRQSVAH RGQSGQWQQ 
      EVEGYERPH VLVTSPGSP ERPRPSPAL APSVGGRIQ 
      IKLGFDPST LQLFVTIVC AAGLTPRSP HQPRSPYCK 
      LFLLPDRSE KSKRRTRTL AGTEPMWNQ TFVYSGLRR 
      ADLRQRALE ITVWDYVRY GANNFLGEA IVELWPLDE 
      QPTWRTLGP HEEVALTPS EHLSPPSTG SRFSDSDTP 
      SECEIDGGR ERRGADGAS VSSVGSSSS PPNERRSRR 
      DQETMQAYG AQGYRRSMA GQRSHSAAP CDSPSVHMS 
      RSRSKSPRH TVTDHRSLS PPDVRIAVA DYQPSYVVP 
      RFQSRSATA TPTGSPKKR QLPQVPQAL ARALQERVA 
      QDLDQDRAG RHRMRYMHT YRSTGSGWE RRYSGLSDS 
      DLANHSRNV RRPVSPDRD RDPLGLVDF DSDMESVGS 
      VTSSAFSTQ SERIRSNKP ASTEYGGGS DRYGYTTSH 
      RSVPRNRER QDLRDPIEP IDQHNHHHH HHHHRESSI 
      KRGQFTRSL SNTEPPPDE KADGSLSDT AVGQIHGLE 
      VPQKEMTRK ESQRERERE RADRQTGQF VGLSKKSNS 
      TSQLSATGR KRRMGFGRR GKTSFTVHR SEEVIPGDV 
      RLSKQGSSA SSDGEGSAD GDRWSPSLR LAGESGQLA 
      DFIDGLGPG QLVGRQVLG APALGEIQL SLCYQKGFL 
      EVEVIRARG LQARPGSKV LPAPYVKVY LVNGKKCIA 
      KAKTSTARR TLDPLYQQQ LAFRENFQG CILQVTVWG 
      DYGRIEGKK VFMGVAQIM LDDLNLSNI VIGWYKLFG 
      TTSLVSGPP SLGLSRRSS MASLDSLKL
    CDS sequence
    • >TC014424 RA
      atggaggaa gggccggat ctgtctcac ctcactccc 
      gaggagcgg gccatcatc gagtccgtc atggtccgg 
      cagaagcag gaggaggag cgcgagcag gagatcatg 
      aggagaaag gccgacgag gtgcacatc ttggaggag 
      acgatacgg gcccgtaac gaacaacac aagaaggcc 
      ggcatcgag ctggacgcc acctgccac atctgccta 
      aagacgaaa ttcgccgac ggcgtcggc cacctgtgc 
      aactactgc tcgatccgg tgctgtgcc cgctgcggg 
      ggcaaagtc accctccgg tcgaataag gtggtgtgg 
      gtttgcatc ctctgcaga aaaaagcaa gaactgttg 
      agtaagact ggccaatgg atgcttggc ggcaccaac 
      gccgacagc gagctgccg agcctccag caggcgccc 
      tccgacaaa cggcccaag ctcgagcgt gcccattcg 
      gccgccgag aaggagaac caacctctt cagcgctcc 
      ggcagcgtg cttcgccga caatactcg gaacaagag 
      acccgcacg ccctcgtgc gagcgctac aaccagcag 
      tataacgag gacgatccg cgtttctac caaggagag 
      ctcgaaggc ctcatgcgg acgcacccc catctcgtc 
      ccgagacgc tatccggac cacgagccc gagcccctg 
      cccatggac atcccccgg atgccctcc gcaagtgcc 
      aacaagaaa cacaagcgc agcggctcg acgaagaaa 
      caccagcac aacccccag ccgcagctg cagcggtcc 
      ttctccagc tcggaggag gacctcaag tccaccccc 
      gagtacggc agcgacgac cgcgacagc gagaaagga 
      gacaacggc gactggcct cccctaagc cactatatc 
      gtcgcccca caacacaga cctctgcag gacgtcacc 
      gccgacaat cggtgcttc accgaacgc agaaagaag 
      acggtcagg tttgaccaa cacgagaac tgggacaga 
      cagggatcg caggactct catagagat tcggggatt 
      gatacctcc tcgaccttc acctccagc gaggattcc 
      actcgcgga gacctgccc aagcacccg ttatcttgg 
      caagtgtcc gccaacgga tgtcgaatg atcggtcat 
      atgatactc cgacgagga tcgagggaa acttccggt 
      tctagtgcg aatatctta ggactgaaa gtggtcgga 
      ggtaaaatc ctcgagaac ggtctcaga ggtgcgatt 
      atcgaaaaa gtcaaaaaa ggaagtatc gccgacatt 
      gaaggccaa ctaaggccg ggtgatgaa gtgattgag 
      tggaatggt cgccctctc caaggaaaa acatatcaa 
      gaagtagcc gatatcata gctgagagc aaacacgaa 
      gcgaatgtt gagttgatc gtttcccga agtatggga 
      agcagacga gccgcccaa gcgacctgg aggcagtct 
      gtggcccat agaggtcag tctggtcaa tggcagcag 
      gaagtggag ggttacgaa agaccgcac gtcctcgta 
      acgtcgccg gggagtccc gaacgaccc agacccagt 
      cccgccttg gctcccagc gtcgggggc agaatacaa 
      atcaagctc ggtttcgac ccctccact ttgcagttg 
      ttcgtcacg atcgtgtgc gcggcgggc ttgacgccg 
      aggtcgccc caccagcca cgcagtcct tactgcaag 
      ctcttcctc ctcccggac cgctccgag aagtccaag 
      cggcggact cgcaccctc gcaggaacg gaaccgatg 
      tggaaccaa accttcgtc tactcgggc cttcgtcga 
      gcggacctg cggcagcgc gccttggag atcaccgtc 
      tgggactat gtccgctac ggcgccaac aatttttta 
      ggcgaggcc atcgtcgaa ttgtggcct ttggacgaa 
      cagcccact tggcggacc cttgggccc cacgaggag 
      gtggccttg acgcccagc gagcacttg tcgcccccg 
      agcaccggc tcgcgcttc tccgactcg gacacgccg 
      tccgagtgc gagatcgac ggcgggagg gagcgtcgc 
      ggcgccgac ggagccagc gtcagcagc gtcggcagc 
      tcatcctcg ccccctaac gagcggaga tccaggaga 
      gaccaagag acgatgcaa gcctacggc gcgcagggg 
      tatcgaaga agcatggcg ggccagagg tcgcactcg 
      gccgcgccg tgcgactcc cccagcgtg cacatgtcg 
      cgctccagg tccaagtcg ccccgacac accgtcacc 
      gaccaccgt tccctgtcg cctcccgac gtccgtatc 
      gccgtagcc gactaccag ccgtcatac gtcgtgcct 
      cgtttccag tcccgttcg gcgactgcg acccccacc 
      ggatcgccg aagaaacgc caactgccc caagtgccc 
      caagcgctg gcgcgcgcc ctccaagag cgcgtggcc 
      caagacctg gaccaggac agggccggg aggcaccgc 
      atgaggtac atgcacacg tacaggagc accggctcg 
      ggatgggaa cgcagatat agtggactg agtgatagt 
      gatttggcg aaccatagc cggaatgtc cgaaggccg 
      gtgtcgccg gacagggac agggacccc ctaggcttg 
      gtggacttt gatagtgac atggaatcg gtcgggtct 
      gtgactagt agtgcgttt tccacgcag tcggaacga 
      atcaggagc aacaaacct gccagtact gaatatggt 
      ggaggtagc gatcgatat gggtacacg acgagtcat 
      cgcagtgtc cccagaaac agagaaaga caagacttg 
      cgggaccct atcgagccc atcgaccaa cacaaccat 
      caccaccac caccatcac cacagggaa agtagcata 
      aaaaggggg cagtttacg cggagcttg tcgaacacg 
      gagccccca ccagacgaa aaagcagat ggcagtctg 
      agtgatact gctgtgggc cagatccac ggtttggaa 
      gtccctcag aaggagatg acgcggaag gagagccag 
      cgggagcgg gaaagggag cgggcggat cgtcagacg 
      ggccagttc gtaggactc agtaaaaag agtaactcg 
      acaagtcag ttgtcggca acaggtagg aaacggagg 
      atgggcttc ggtcgaagg ggcaagacc tcgttcact 
      gttcatcga tcggaggag gttattccc ggagatgtg 
      aggctgtcc aagcaaggc tcctcggct tccagtgac 
      ggagaaggg tcggccgac ggcgacagg tggtcaccc 
      tctttaaga ctggcgggc gagagtggg caactggcc 
      gacttcatc gacggtttg ggaccagga caactagta 
      ggacgacaa gtgctggga gcaccggct ttaggcgaa 
      atccaactg agtctctgc taccaaaag gggttcctc 
      gaagttgag gtgatacga gcgcgggga ctacaagcc 
      agacccgga tccaaagtt ttaccagca ccctacgtc 
      aaggtctat ctagtcaac ggcaaaaaa tgcatagcc 
      aaggccaaa acctcgacg gctcgacga actttagat 
      ccgctctac cagcaacag ttagctttc agagagaat 
      ttccaaggc tgtatactc caggtgacc gtctggggt 
      gactacggg cgcatcgag ggcaagaaa gtgttcatg 
      ggcgtggcc caaatcatg ctcgatgac ctcaacttg 
      agcaacatc gtcatcgga tggtacaaa ctcttcggc 
      accacatcc ctggttagt ggacctcct tcgctcggt 
      ctctctcga cgcagctcc atggctagc ttagattcc 
      ctcaagttg taa
    mRNA sequence
    • >TC014424 RA
      tattattta ttaaaggta catgaatgg gtaattgca 
      aactgtgat atttggccg gacgacggc tgagacaat 
      cgaaaaaaa tcgcgaaca agtcggtgc gaaggtccg 
      tgtgcgcta gggccgcgt cgcgagtgc cccgagagg 
      ccgtcgcca gggagccgc ctgtggaca tagccgcat 
      gttgttgca tttcggtgc cgctctgag atggacgac 
      gtgaagccg cggcgcgac cgcgacctc tccaacggg 
      tagtcggtg gcatgtccg gaaagggct agcatggag 
      gaagggccg gatctgtct cacctcact cccgaggag 
      cgggccatc atcgagtcc gtcatggtc cggcagaag 
      caggaggag gagcgcgag caggagatc atgaggaga 
      aaggccgac gaggtgcac atcttggag gagacgata 
      cgggcccgt aacgaacaa cacaagaag gccggcatc 
      gagctggac gccacctgc cacatctgc ctaaagacg 
      aaattcgcc gacggcgtc ggccacctg tgcaactac 
      tgctcgatc cggtgctgt gcccgctgc gggggcaaa 
      gtcaccctc cggtcgaat aaggtggtg tgggtttgc 
      atcctctgc agaaaaaag caagaactg ttgagtaag 
      actggccaa tggatgctt ggcggcacc aacgccgac 
      agcgagctg ccgagcctc cagcaggcg ccctccgac 
      aaacggccc aagctcgag cgtgcccat tcggccgcc 
      gagaaggag aaccaacct cttcagcgc tccggcagc 
      gtgcttcgc cgacaatac tcggaacaa gagacccgc 
      acgccctcg tgcgagcgc tacaaccag cagtataac 
      gaggacgat ccgcgtttc taccaagga gagctcgaa 
      ggcctcatg cggacgcac ccccatctc gtcccgaga 
      cgctatccg gaccacgag cccgagccc ctgcccatg 
      gacatcccc cggatgccc tccgcaagt gccaacaag 
      aaacacaag cgcagcggc tcgacgaag aaacaccag 
      cacaacccc cagccgcag ctgcagcgg tccttctcc 
      agctcggag gaggacctc aagtccacc cccgagtac 
      ggcagcgac gaccgcgac agcgagaaa ggagacaac 
      ggcgactgg cctccccta agccactat atcgtcgcc 
      ccacaacac agacctctg caggacgtc accgccgac 
      aatcggtgc ttcaccgaa cgcagaaag aagacggtc 
      aggtttgac caacacgag aactgggac agacaggga 
      tcgcaggac tctcataga gattcgggg attgatacc 
      tcctcgacc ttcacctcc agcgaggat tccactcgc 
      ggagacctg cccaagcac ccgttatct tggcaagtg 
      tccgccaac ggatgtcga atgatcggt catatgata 
      ctccgacga ggatcgagg gaaacttcc ggttctagt 
      gcgaatatc ttaggactg aaagtggtc ggaggtaaa 
      atcctcgag aacggtctc agaggtgcg attatcgaa 
      aaagtcaaa aaaggaagt atcgccgac attgaaggc 
      caactaagg ccgggtgat gaagtgatt gagtggaat 
      ggtcgccct ctccaagga aaaacatat caagaagta 
      gccgatatc atagctgag agcaaacac gaagcgaat 
      gttgagttg atcgtttcc cgaagtatg ggaagcaga 
      cgagccgcc caagcgacc tggaggcag tctgtggcc 
      catagaggt cagtctggt caatggcag caggaagtg 
      gagggttac gaaagaccg cacgtcctc gtaacgtcg 
      ccggggagt cccgaacga cccagaccc agtcccgcc 
      ttggctccc agcgtcggg ggcagaata caaatcaag 
      ctcggtttc gacccctcc actttgcag ttgttcgtc 
      acgatcgtg tgcgcggcg ggcttgacg ccgaggtcg 
      ccccaccag ccacgcagt ccttactgc aagctcttc 
      ctcctcccg gaccgctcc gagaagtcc aagcggcgg 
      actcgcacc ctcgcagga acggaaccg atgtggaac 
      caaaccttc gtctactcg ggccttcgt cgagcggac 
      ctgcggcag cgcgccttg gagatcacc gtctgggac 
      tatgtccgc tacggcgcc aacaatttt ttaggcgag 
      gccatcgtc gaattgtgg cctttggac gaacagccc 
      acttggcgg acccttggg ccccacgag gaggtggcc 
      ttgacgccc agcgagcac ttgtcgccc ccgagcacc 
      ggctcgcgc ttctccgac tcggacacg ccgtccgag 
      tgcgagatc gacggcggg agggagcgt cgcggcgcc 
      gacggagcc agcgtcagc agcgtcggc agctcatcc 
      tcgccccct aacgagcgg agatccagg agagaccaa 
      gagacgatg caagcctac ggcgcgcag gggtatcga 
      agaagcatg gcgggccag aggtcgcac tcggccgcg 
      ccgtgcgac tcccccagc gtgcacatg tcgcgctcc 
      aggtccaag tcgccccga cacaccgtc accgaccac 
      cgttccctg tcgcctccc gacgtccgt atcgccgta 
      gccgactac cagccgtca tacgtcgtg cctcgtttc 
      cagtcccgt tcggcgact gcgaccccc accggatcg 
      ccgaagaaa cgccaactg ccccaagtg ccccaagcg 
      ctggcgcgc gccctccaa gagcgcgtg gcccaagac 
      ctggaccag gacagggcc gggaggcac cgcatgagg 
      tacatgcac acgtacagg agcaccggc tcgggatgg 
      gaacgcaga tatagtgga ctgagtgat agtgatttg 
      gcgaaccat agccggaat gtccgaagg ccggtgtcg 
      ccggacagg gacagggac cccctaggc ttggtggac 
      tttgatagt gacatggaa tcggtcggg tctgtgact 
      agtagtgcg ttttccacg cagtcggaa cgaatcagg 
      agcaacaaa cctgccagt actgaatat ggtggaggt 
      agcgatcga tatgggtac acgacgagt catcgcagt 
      gtccccaga aacagagaa agacaagac ttgcgggac 
      cctatcgag cccatcgac caacacaac catcaccac 
      caccaccat caccacagg gaaagtagc ataaaaagg 
      gggcagttt acgcggagc ttgtcgaac acggagccc 
      ccaccagac gaaaaagca gatggcagt ctgagtgat 
      actgctgtg ggccagatc cacggtttg gaagtccct 
      cagaaggag atgacgcgg aaggagagc cagcgggag 
      cgggaaagg gagcgggcg gatcgtcag acgggccag 
      ttcgtagga ctcagtaaa aagagtaac tcgacaagt 
      cagttgtcg gcaacaggt aggaaacgg aggatgggc 
      ttcggtcga aggggcaag acctcgttc actgttcat 
      cgatcggag gaggttatt cccggagat gtgaggctg 
      tccaagcaa ggctcctcg gcttccagt gacggagaa 
      gggtcggcc gacggcgac aggtggtca ccctcttta 
      agactggcg ggcgagagt gggcaactg gccgacttc 
      atcgacggt ttgggacca ggacaacta gtaggacga 
      caagtgctg ggagcaccg gctttaggc gaaatccaa 
      ctgagtctc tgctaccaa aaggggttc ctcgaagtt 
      gaggtgata cgagcgcgg ggactacaa gccagaccc 
      ggatccaaa gttttacca gcaccctac gtcaaggtc 
      tatctagtc aacggcaaa aaatgcata gccaaggcc 
      aaaacctcg acggctcga cgaacttta gatccgctc 
      taccagcaa cagttagct ttcagagag aatttccaa 
      ggctgtata ctccaggtg accgtctgg ggtgactac 
      gggcgcatc gagggcaag aaagtgttc atgggcgtg 
      gcccaaatc atgctcgat gacctcaac ttgagcaac 
      atcgtcatc ggatggtac aaactcttc ggcaccaca 
      tccctggtt agtggacct ccttcgctc ggtctctct 
      cgacgcagc tccatggct agcttagat tccctcaag 
      ttgtaacac tgaccattc cattgtttc acacacact 
      gactcgcgc ctgctattt ctatttgtc acattatca 
      ctccctcat tatcatcat agtgtcacc gatcctcgt 
      agtctacgc aactcttcc acttcgctt aggtacgcc 
      taggtttga gtgaacacg ctgtatatt tgcttagaa 
      ctgttagat catcctttc tgcccccgg gtttggtgc 
      aaatgtttt ctgatgatt tttttgcag tgagggcga 
      attccggcg tggttggag gcgacgtga cggcgagag 
      aaagaagac agtcgccgg aatattcgc cggtagtgc 
      gacaaactt tgcaaaaac atatacatg agactatat 
      tccgattta atgcatttc cggcgaata ttaatgaaa 
      aatattgta cttatcaga gtattacgg tatggtgcg 
      aaaattatt agctgacat ttagaagca gattaaaat 
      agatacata tagtgtaaa tgaagtgaa aaaagatat 
      ataagtgta gatatcacg cacacatat aaatatagt 
      atcacataa aatatcata tctaaaata tttgttata 
      tgacgtctg gggcggccc aaaaaacta ccacctaga 
      ttcatatgc gcgtgatta caacgatcc acttaaaaa 
      tacaacaaa tgacttgta tttttctgg gacgtcctg 
      ttcaaacaa caaaaatgc ttcattgga attgataaa 
      ttgcttttt tacttgaaa ctaagtaat gacatgaaa 
      ttaggtcgt gttaatgaa ctatgtaaa ttatgataa 
      tgacattgt agaactgta tatgaacgt ttaattgta 
      ccatataat ttattcttg attgttgaa ttgcatgtg 
      atataactt gaagatttt atgttggct gtttgaatt 
      tcccgcttc gataagata atctccgtt ctgaagcac 
      aggtgtcgc cacgtttcc gtttcgttg tgtataaaa 
      catataaca tcgaaaact gtaatgtaa tcgaagttt 
      cccgcgatt cgatttcag aatattctc tcatttttg 
      taacgtttg taatgaatt tttagaaag ggggcgtgt 
      cgctgatat tcgactgtt tttccgcag ccgactgct 
      acgacaccg cggtgtttt tactaccga ttcggcaaa 
      cagtgccaa atcgggtat taaaaatgg ccggttttt 
      taaattgtt tagtaaaac aatccgaat aatgcttct 
      aagtcatag taaaagctt taatctgct tgaaaccca 
      aaatatatc atacgtaac aacaagaat aagattttc 
      cgaaacaac tataaccat aattatgat tgtatataa 
      ttttcgaag aattgggtt caactaaaa tgtaataca 
      ctgcataca gttcagatg gttgattta aatattata 
      aaagatatc aatagttag tttttctac gctgttctt 
      taataaaat aagacaccc acttcatgg atcatgtac 
      tcccatttg ttccaaggt ccgaactgt attgtacct 
      tttatcatt ccgattaaa tcattatca ataaccgag 
      tgtagatat gcgccttac atactgttc gcagcagcc 
      agtccggcc ctgcgtcac ttttcaata aaggcccgt 
      gtaaattgc atcaattgg actaaaatg cggcaagta 
      tggcgtcgg actggctcg ttgcacttt tgtatctgt 
      accggacct tgaagggtt aagagacga cgaacaaag 
      ctgtcattt gcgcttgag acacttcgg ctactgaac 
      tgtatttta gtgtacggt cctttgaca atttgcgca 
      tatcactgg aagtgtcag aagcgtcca ctgtatgtc 
      cgtcttgat atgttcact cttgactga tgtatccat 
      tttatccaa gcccatggg caagacaat aaatgaatg 
      atctatcc
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