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Gene information
General information
TC013642 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2491969..2524886
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013642
    iBeetle screen iB_08327

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary - Number of animals showing the phenotype: 1

    mature egg not present - Number of animals showing the phenotype: 2

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013642 RA
      MGLLPLLLL LAAAHADLD AERKLIRIR GDIILGGIF 
      PMHEQVPGR SDMPCGAVK EEKGMQRLE AMLYAIDEI 
      NASPDLLPN VTLGALVID SCSSDTYAL EQSMEFVRS 
      YMNQDMSEY KCESGKPPV YVPHKPVTA VIGASFSVV 
      SIMVANILR LFKIPQVSY ASTSTELSD KSRFEYFSR 
      VVPPDNFQA QTMVEIIKE LGWKYVSTV AVEGDYGER 
      GIASFISYS ADAGICIAV TEKILRNSK TEDFDRIIE 
      RLLQKTARA VVLFVDEDN TRKLLQATI RANKTGHFL 
      WIGSDSWGA KVHPVRDQE FAAEGAITI LPHRNAIQG 
      TKSYIYIRT QTGRSPGSN TSITRNTTI Y
    CDS sequence
    • >TC013642 RA
      atggggctg ctcccgctc ctcctgctg ctggcggcg 
      gcccacgcg gacctcgac gccgagcgg aagctcatc 
      cgcatcagg ggcgacatc atcctcggc ggcatcttc 
      cccatgcac gagcaggtc cccggacgc tccgacatg 
      ccctgcggc gccgtcaaa gaggagaaa ggcatgcag 
      cgcctagag gccatgctc tacgccatc gacgaaatc 
      aacgcgagt cccgactta ctcccaaat gtcaccctc 
      ggtgctctt gtcatcgat tcctgcagc agcgatacc 
      tacgctctc gagcagtcc atggagttc gtccgctcc 
      tacatgaac caggacatg tcggagtac aagtgtgag 
      tctggaaag ccgcccgtc tatgtgcca cataaaccg 
      gttacagct gtcattggg gcttctttt agcgtggtt 
      tcgatcatg gtggcaaat atcttgcgg ttatttaag 
      atcccccaa gtcagttac gcctctacc agcaccgaa 
      ttgtccgac aagtcgaga ttcgaatat ttcagcaga 
      gttgtgccg cccgataat ttccaagcg caaacgatg 
      gtggaaatc atcaaagaa ctgggctgg aagtacgtg 
      tccacagtg gcagtcgaa ggagattat ggagaaaga 
      gggatagcg tcttttata tcttattca gccgatgcc 
      ggaatctgc atagcggta accgaaaaa attctccgg 
      aattctaaa accgaagac ttcgacaga attatagag 
      cgacttctc caaaagacg gctcgtgca gtcgtcctc 
      ttcgtcgac gaggacaac acgagaaag ctgctgcaa 
      gcaacaatt cgtgcgaat aagacagga catttcctt 
      tggatcggc agtgattcg tggggagct aaagtacac 
      cccgtcagg gaccaggag ttcgctgct gagggagca 
      atcaccatc ctaccccac aggaatgct attcaaggt 
      actaaaagc tatatttat atacgaaca cagaccggt 
      cgcagtccc ggatcaaat acttcgata actcgcaac 
      accaccatt tattaa
    mRNA sequence
    • >TC013642 RA
      cagtctagc gcaagtttc cgtcgagga gccctaaga 
      tgagcgcgc tgcgccgcc aaatctgag tctggactc 
      ttgcgcact ttggcctag ccgcgcgtg gacccacca 
      tgagaccat ctccaagtg ttccgctta actgaacaa 
      ttaattaaa acttgattg cgatttaat atcgatccc 
      ggcctaatt ggtgcttaa atttgtggg tgtcggggc 
      actatgggg ctgctcccg ctcctcctg ctgctggcg 
      gcggcccac gcggacctc gacgccgag cggaagctc 
      atccgcatc aggggcgac atcatcctc ggcggcatc 
      ttccccatg cacgagcag gtccccgga cgctccgac 
      atgccctgc ggcgccgtc aaagaggag aaaggcatg 
      cagcgccta gaggccatg ctctacgcc atcgacgaa 
      atcaacgcg agtcccgac ttactccca aatgtcacc 
      ctcggtgct cttgtcatc gattcctgc agcagcgat 
      acctacgct ctcgagcag tccatggag ttcgtccgc 
      tcctacatg aaccaggac atgtcggag tacaagtgt 
      gagtctgga aagccgccc gtctatgtg ccacataaa 
      ccggttaca gctgtcatt ggggcttct tttagcgtg 
      gtttcgatc atggtggca aatatcttg cggttattt 
      aagatcccc caagtcagt tacgcctct accagcacc 
      gaattgtcc gacaagtcg agattcgaa tatttcagc 
      agagttgtg ccgcccgat aatttccaa gcgcaaacg 
      atggtggaa atcatcaaa gaactgggc tggaagtac 
      gtgtccaca gtggcagtc gaaggagat tatggagaa 
      agagggata gcgtctttt atatcttat tcagccgat 
      gccggaatc tgcatagcg gtaaccgaa aaaattctc 
      cggaattct aaaaccgaa gacttcgac agaattata 
      gagcgactt ctccaaaag acggctcgt gcagtcgtc 
      ctcttcgtc gacgaggac aacacgaga aagctgctg 
      caagcaaca attcgtgcg aataagaca ggacatttc 
      ctttggatc ggcagtgat tcgtgggga gctaaagta 
      caccccgtc agggaccag gagttcgct gctgaggga 
      gcaatcacc atcctaccc cacaggaat gctattcaa 
      ggtactaaa agctatatt tatatacga acacagacc 
      ggtcgcagt cccggatca aatacttcg ataactcgc 
      aacaccacc atttattaa ctctttgat ctcgcattc 
      acactatta cctactaca actaaaatc gctctcaga 
      cgtgctatc gctgaaaaa agcgacgct gctgaagca 
      ctaaatgta gaatgcgcc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY