Status
Please wait ...
Gene information
General information
TC013469 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9119340..9122509
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013469
    iBeetle screen iB_08299

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    About 65% larvae are belongs to second instar larva stage.

    pygopodium potentially ectopicallystructure present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013469 RA
      MESEPVPQP LRHGCDDLW TWNRWERSP EVRLYGNNF 
      RIAHFHPNW SSGTAGVRG TRVLNNGRY FWELHISRR 
      IFGTSMMFG IGTKKARLH ADSFTNLLG EDDHGWGLS 
      HKGLLWHGG RWTHYTKPF RENMATKIG VLFDGIAGT 
      LSYYKDEKY LGVAFRGLN EVKEPLYPI ICSTAAKTE 
      MYLDSMKRD FVNLQDRCR AVIVKRIRS KQDLEKLFV 
      PTKIRSYLA EAIVDSVEP FKPVNHNTI SINVGNM
    CDS sequence
    • >TC013469 RA
      atggagtcg gaaccggtc ccccagccc ctgcggcac 
      ggctgcgac gacctgtgg acgtggaac cggtgggag 
      cgcagcccc gaggtccgg ctctacggc aacaacttc 
      cgcatcgcg cacttccac cccaactgg agcagcggc 
      acggccggc gtgcggggc acccgggtg ctgaacaac 
      ggccgctac ttctgggag ctgcacatc tcgcggcgc 
      atcttcggc accagcatg atgttcggc atcggcacc 
      aagaaggcg cgcctgcac gccgactcc ttcaccaac 
      ctgctcggc gaagacgac cacggctgg gggctgtcg 
      cacaagggg ctgctgtgg cacggcggc cgctggacg 
      cactacacc aagcccttc cgggagaac atggccacc 
      aagatcggc gtgctcttc gacggcatc gccggcacg 
      ctgagctac tacaaggac gagaagtac ctgggggtg 
      gccttccgg gggctcaac gaggtcaag gagcccctt 
      taccccatt atttgctcc acggccgcc aagactgag 
      atgtatctg gattcgatg aagagggac ttcgtcaat 
      ttgcaggac aggtgcagg gcggtgatc gtcaagagg 
      attaggagt aagcaggat ttggaaaaa ttgttcgtt 
      ccgactaaa attaggagt tatttggct gaggcgatt 
      gttgacagt gttgagccg ttcaaaccc gtcaatcac 
      aacacgatt tcgattaat gtcgggaat atgtag
    mRNA sequence
    • >TC013469 RA
      tcactccgc agagtgtcc gaaatcatt aattgttac 
      cctatttga atatcacag aaaaatgac caacgtgca 
      gcccaagaa cgtcgagag ggaattcag gatttgcgg 
      gattagacg cgtggagga cgtgccccc cgcgtggga 
      ggagtcctc cgcatagca atcgaagcg ttccgactc 
      catggagtc ggaaccggt cccccagcc cctgcggca 
      cggctgcga cgacctgtg gacgtggaa ccggtggga 
      gcgcagccc cgaggtccg gctctacgg caacaactt 
      ccgcatcgc gcacttcca ccccaactg gagcagcgg 
      cacggccgg cgtgcgggg cacccgggt gctgaacaa 
      cggccgcta cttctggga gctgcacat ctcgcggcg 
      catcttcgg caccagcat gatgttcgg catcggcac 
      caagaaggc gcgcctgca cgccgactc cttcaccaa 
      cctgctcgg cgaagacga ccacggctg ggggctgtc 
      gcacaaggg gctgctgtg gcacggcgg ccgctggac 
      gcactacac caagccctt ccgggagaa catggccac 
      caagatcgg cgtgctctt cgacggcat cgccggcac 
      gctgagcta ctacaagga cgagaagta cctgggggt 
      ggccttccg ggggctcaa cgaggtcaa ggagcccct 
      ttaccccat tatttgctc cacggccgc caagactga 
      gatgtatct ggattcgat gaagaggga cttcgtcaa 
      tttgcagga caggtgcag ggcggtgat cgtcaagag 
      gattaggag taagcagga tttggaaaa attgttcgt 
      tccgactaa aattaggag ttatttggc tgaggcgat 
      tgttgacag tgttgagcc gttcaaacc cgtcaatca 
      caacacgat ttcgattaa tgtcgggaa tatgtaggc 
      gtttgattc gacaagtga atcacgtgg ataaataaa 
      aggcaaact aaatacttt tattgttgg tccacactg 
      tatatgtat ttgtaccaa aaggttttc tcggtacgt 
      gtgtataga aatgtaaaa ccgtggatt tgtccatgt 
      gccatttta aaattctgt aaatacgat aaatgggac 
      gacgccagt ggtgaatgt tacctagag tctagtcgt 
      agtgttaag ctattttat caatgtaaa atggggaat 
      tttttcaaa tgaattaat caacatttt tatactcat 
      gacacgatt tttcttatt gattgaact gctaatagc 
      gtaatacga aacgattta ttgtgattg attatctag 
      tcttgtttt gttgttgtt tttcgtgtg ttgtttgtt 
      attggtttg tatgagttt gagaaagtt gcacaaaaa 
      agtgtgtac attttggcc agtttgtgc aatagaaat 
      gtaaataaa taaaattca tttctttag gttcgtaag 
      actgtaatg actaaattg atgtttagg gcaattgaa 
      tacattatt ttttaaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY