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Gene information
General information
TC014321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9036771..9038571
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014321
    iBeetle screen iB_08296

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014321 RA
      MFFFFRDEK LIREAIERS DFLNKILKG DLLKEVVDA 
      MYPREIKSS EVIIKEGEK GAHMYVSAG GQYEVSVKG 
      KGVVNTFED IRIFGEMAI LYNDKRNAT IKAIKAGKV 
      WVLDRTIYQ KLMLQFNIK EQDEVLSFL ENVPTLNKV 
      GTKVLQKVT TLLKVKYFA PGATIVKQG DRGDKFYII 
      RAGTVTISK EGEGVVGNY GKGQYFGEL ALLDEEFRQ 
      ATVTADAPE GVECLTLKR QHFRDHFGR ITEGGTVEY 
      TPKSGPKRS ESVREHQDI ELKDLTILT TLGVGGFGR 
      VELVQHKSK NLIFALKYL KKFDMVEQN QQEHVYNER 
      NIQLECRSK FIVRLYRSY KDARYIYFL MESCLGGDL 
      WTLLQRQKT RRFDEKDSR FIAGCVLEA FTYLHERDI 
      VYRDLKPEN LLIDEHGYI KLTDFGFAK RLGARGRTF 
      TFAGTPEYV APEIILSRG HDKGVDYWA FGIFIFELL 
      TGRTPFRTN DASHMRTYN KILTGIDSV EFPSYVSSK 
      ARNIIEKLC RAIPSERLG CQRNGVKDI KSHRWFLGF 
      DWVKLNEGK LTAPFKPKL KSSTDTRYF EQFKKDNDC 
      PPEETSGWD ENF
    CDS sequence
    • >TC014321 RA
      atgtttttt ttcttcaga gatgaaaaa ttgattcgg 
      gaagcgatc gaacggagc gacttcctc aacaagatt 
      ttaaaaggg gacttgttg aaagaagtg gtcgatgcc 
      atgtacccc cgcgagatc aaatcgtcc gaagtcata 
      ataaaggag ggcgagaaa ggggcccac atgtatgtc 
      tcggccggc ggccagtac gaagtctcc gtcaagggc 
      aaaggcgtg gtgaatacg ttcgaggat atccgaatt 
      ttcggcgag atggcaatc ctgtacaat gataaaaga 
      aacgcgacc attaaagca ataaaagcc gggaaagtc 
      tgggttttg gacaggacc atttaccag aaactgatg 
      ctccagttc aacatcaag gagcaggac gaggtgctt 
      tccttcctc gaaaacgtc ccaactttg aataaagtc 
      gggaccaaa gtcttgcaa aaggtcacg actttgctc 
      aaagttaag tacttcgcg ccgggggcc acgatcgtc 
      aaacagggt gaccgaggc gacaagttt tacataatc 
      cgggccggc acggtgacc atctcgaag gagggcgaa 
      ggcgtggtg gggaactac ggcaaaggc cagtatttc 
      ggcgaattg gcgcttctg gacgaggag tttcgccaa 
      gccaccgtc acagccgac gcccccgaa ggggtggag 
      tgcctcaca ctgaaacgg cagcacttc cgcgaccac 
      ttcgggagg ataacagag ggcggaacc gtggaatac 
      acccccaag tcggggccc aaacggagc gaatccgtg 
      cgcgaacac caagacatc gaactcaag gatttaaca 
      attctgacg acgctgggc gtcggaggg ttcgggcgc 
      gtcgagctc gtccaacac aagtccaaa aacctgatc 
      ttcgcgtta aaatacttg aaaaagttt gatatggtc 
      gagcagaac caacaagaa catgtttac aacgagcga 
      aacatccag ttagagtgt cgctcgaaa ttcattgta 
      cgcctttac cgcagttac aaagacgcg cgttatatt 
      tatttcctc atggagtcg tgtctgggg ggcgacctg 
      tggaccctc ctccagagg caaaagacg cgccgcttc 
      gacgagaaa gactccagg tttatcgcc ggctgcgtc 
      ctcgaagct ttcacatac ctccacgaa agagacata 
      gtctacaga gacctcaag ccggaaaac ttgctcata 
      gacgaacac ggttatatc aaattgacc gatttcggg 
      ttcgccaag cgcctgggg gcccgtggc cgaactttc 
      acgttcgca gggacgccc gagtacgtg gcccctgaa 
      attattcta agtcgggga catgacaag ggcgtcgat 
      tattgggct tttggtatt ttcattttc gagctgttg 
      accgggaga actccgttc cggacgaac gatgcgagt 
      catatgagg acgtacaat aaaatttta accgggatt 
      gacagtgtc gaattcccg agttatgtc tcgtccaaa 
      gcccgcaat attatcgag aagctatgc agggccatc 
      ccgagcgaa cgattaggg tgtcaaagg aacggagtt 
      aaagatatt aaatcccac cggtggttc ctgggcttt 
      gattgggtc aaactcaac gaaggcaaa ctgacagcg 
      ccctttaaa cccaaactc aagtccagc accgacacg 
      agatatttc gaacaattc aaaaaggac aacgactgt 
      ccgccggaa gaaacaagt ggatgggat gaaaatttc 
      tag
    mRNA sequence
    • >TC014321 RA
      attttgaat gtttttttt cttcagaga tgaaaaatt 
      gattcggga agcgatcga acggagcga cttcctcaa 
      caagatttt aaaagggga cttgttgaa agaagtggt 
      cgatgccat gtacccccg cgagatcaa atcgtccga 
      agtcataat aaaggaggg cgagaaagg ggcccacat 
      gtatgtctc ggccggcgg ccagtacga agtctccgt 
      caagggcaa aggcgtggt gaatacgtt cgaggatat 
      ccgaatttt cggcgagat ggcaatcct gtacaatga 
      taaaagaaa cgcgaccat taaagcaat aaaagccgg 
      gaaagtctg ggttttgga caggaccat ttaccagaa 
      actgatgct ccagttcaa catcaagga gcaggacga 
      ggtgctttc cttcctcga aaacgtccc aactttgaa 
      taaagtcgg gaccaaagt cttgcaaaa ggtcacgac 
      tttgctcaa agttaagta cttcgcgcc gggggccac 
      gatcgtcaa acagggtga ccgaggcga caagtttta 
      cataatccg ggccggcac ggtgaccat ctcgaagga 
      gggcgaagg cgtggtggg gaactacgg caaaggcca 
      gtatttcgg cgaattggc gcttctgga cgaggagtt 
      tcgccaagc caccgtcac agccgacgc ccccgaagg 
      ggtggagtg cctcacact gaaacggca gcacttccg 
      cgaccactt cgggaggat aacagaggg cggaaccgt 
      ggaatacac ccccaagtc ggggcccaa acggagcga 
      atccgtgcg cgaacacca agacatcga actcaagga 
      tttaacaat tctgacgac gctgggcgt cggagggtt 
      cgggcgcgt cgagctcgt ccaacacaa gtccaaaaa 
      cctgatctt cgcgttaaa atacttgaa aaagtttga 
      tatggtcga gcagaacca acaagaaca tgtttacaa 
      cgagcgaaa catccagtt agagtgtcg ctcgaaatt 
      cattgtacg cctttaccg cagttacaa agacgcgcg 
      ttatattta tttcctcat ggagtcgtg tctgggggg 
      cgacctgtg gaccctcct ccagaggca aaagacgcg 
      ccgcttcga cgagaaaga ctccaggtt tatcgccgg 
      ctgcgtcct cgaagcttt cacatacct ccacgaaag 
      agacatagt ctacagaga cctcaagcc ggaaaactt 
      gctcataga cgaacacgg ttatatcaa attgaccga 
      tttcgggtt cgccaagcg cctgggggc ccgtggccg 
      aactttcac gttcgcagg gacgcccga gtacgtggc 
      ccctgaaat tattctaag tcggggaca tgacaaggg 
      cgtcgatta ttgggcttt tggtatttt cattttcga 
      gctgttgac cgggagaac tccgttccg gacgaacga 
      tgcgagtca tatgaggac gtacaataa aattttaac 
      cgggattga cagtgtcga attcccgag ttatgtctc 
      gtccaaagc ccgcaatat tatcgagaa gctatgcag 
      ggccatccc gagcgaacg attagggtg tcaaaggaa 
      cggagttaa agatattaa atcccaccg gtggttcct 
      gggctttga ttgggtcaa actcaacga aggcaaact 
      gacagcgcc ctttaaacc caaactcaa gtccagcac 
      cgacacgag atatttcga acaattcaa aaaggacaa 
      cgactgtcc gccggaaga aacaagtgg atgggatga 
      aaatttcta gtactcaaa ataatttta gttaggtct 
      g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY