General information | |||
TC031354
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 2117833..2124427 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC031354 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08292 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larva segment central constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: Constriction of abdomen segment is major phenotype but with this phenotype there is also head & thorax appendages shape irregular & larvae segements are randomly partially not present in all three tagmata. |
thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031354 RA
MCPLVFLFL ISVTTIREI FSQNLINED SSFLSQLFN VSINNIAAV ANGFSFINI PTDNVKFFL FERTSNQTI TLNRDDHLE HLVPSDPTI LITHGWTDQ ETASWIQDM AFLYHKKRN YNVIAVDWS IDADKNYIY SSSATQSVG IIIGAFLIQ VSKHVENFF EKVNLIGHS LGAQVVGFA GKYVENSTD SKLDRITGL DAASPLFET PILRPPELR LADSDANFV DLIHTALGV GVTTIREIF SQNLINEDS SFLSQLFNV SINNIAAVA NGFSFINIP TDNVKFLLF ERTSNQTIT LNHDDPLEH LVPSDPTIL ITHGWTDQG TASWIQDMA FLYHKKGNY NVIAVDWSI DADRNYIYS SSATQSVGI IIGAFLIQV SKKVDNFFE KVHLIGHSL GAQVVGFAG KYVENSTDS KLDRITGLD AASPLFETP ILRPPELRL ADTDASFVD LIHTALGVG YIGAFGTAD FYVNGGIIQ LNCTDDLTD IASCNHQSS HIYYSETIL ERKKYEATK CEDAVRFNL GLCDDNDNA YMGDEIEML KTLTCRSLT HRSSGYLTL LYKSKMSQN TVPVETSIR GKLSSELQT THLEVINES YMHNVPKGA ETHFKVVVV SEKFSDLPL IKRHRLVND ILKEELQSG VHALSIVAK TPSQWSSSE QIIEPSPNC RGGFGK
- >TC031354 RA
atgtgtcca ctggtattt ctcttcttg attagtgtg acaacaatt agggaaatt ttttcccaa aatttgatt aacgaagac tcctctttc ttgagccaa ctcttcaac gtttcgatc aacaacatc gcagctgtt gctaatggt ttttccttc atcaacatc ccaacagat aacgttaaa ttcttttta ttcgaacgc actagcaat caaactatc actctcaac cgtgacgac catctggag cacctagta ccatcagat ccgaccatt ttaataact catgggtgg acagatcag gaaacagca tcatggatc caagacatg gcatttttg taccataaa aaacgcaac tacaacgtt attgcagtg gactggtca atcgatgcc gacaaaaac tacatctac tcatcatca gcaactcaa tctgttggt atcattatt ggtgctttc ctaatccaa gtctcaaaa catgtggaa aactttttc gaaaaagtt aacttaatc gggcattct ttaggcgcc caagttgta ggatttgca ggaaagtac gtggaaaac tccacagat tccaaactt gatagaatc acgggttta gacgctgca agccccctt ttcgaaacc ccaattttg aggccccct gaactgcga ttggctgac agcgatgcc aattttgtt gatcttatt cacacagct ttaggagtt ggtgtgaca acaattagg gaaattttt tcccaaaat ttgattaac gaagactcc tcttttttg agccaactc ttcaacgtt tcgatcaac aacatcgca gctgttgct aatggtttt tccttcatc aacatccca acagataac gttaaattc cttttattc gaacgcact agcaatcaa actatcact ctcaaccat gacgaccct ctggagcac ttagtacca tcagatccg accatttta ataactcat gggtggaca gatcaggga acagcatca tggatccaa gacatggca tttttgtac cataaaaaa ggcaactac aacgttatt gcagtggat tggtcaatc gatgctgac agaaactac atctactca tcatcagca acccaatct gttggtatc attattggt gctttccta atccaagtc tcaaaaaaa gtggacaac tttttcgaa aaagttcac ctaattggg cattcttta ggtgcccaa gttgtagga tttgcagga aagtacgtg gaaaactcc acagattcc aaacttgat agaatcaca ggtctagac gctgcaagc ccccttttt gaaaccccc attttgaga ccccctgaa ctgcgattg gctgacacc gatgccagt tttgttgat cttattcac acagcttta ggggttggg tatattggg gcttttgga accgccgac ttctatgtc aatgggggt attattcaa ttaaattgt acagacgac ttgaccgat attgcgtct tgtaatcac cagagctca catatttac tattcggag acaatatta gagcgaaaa aagtacgaa gcaaccaaa tgtgaggat gctgtaagg tttaatttg gggttgtgt gacgataac gacaatgct tatatgggc gatgagatt gaaatgttg aaaacttta acgtgtcgc tcactcaca catcgatca tcaggttat ctaaccctg ctttacaaa tccaaaatg tcgcaaaat acagtccct gttgagact tcaattcgt ggaaaactc agttcagag ctacaaact acccacttg gaggtcatc aacgagtca tacatgcac aacgtgcct aaaggcgcc gagacacat ttcaaagtt gtggtggtc tctgagaaa ttttcagac ctgccttta atcaagaga cacagattg gtaaacgac attttaaag gaagaattg caaagtgga gtccatgct ctctcgatt gttgccaaa accccttca cagtggagc agcagtgag caaataatc gaacccagt cctaactgt aggggcggc ttcggcaaa tga
- >TC031354 RA
gatgatatc tatgtttcg aatatttgt cagcggttt aattattac tacgcatgg aaaatttaa aatatattt taaccataa atcagaata tataaatta aaataaatt tttgccatt ttcgatgcg aattttatt tagttacct agaaaaagt tatgcaaag taaatcaaa acatgtaat aatatcgag aaacgtgca caatgtgtc cactggtat ttctcttct tgattagtg tgacaacaa ttagggaaa ttttttccc aaaatttga ttaacgaag actcctctt tcttgagcc aactcttca acgtttcga tcaacaaca tcgcagctg ttgctaatg gtttttcct tcatcaaca tcccaacag ataacgtta aattctttt tattcgaac gcactagca atcaaacta tcactctca accgtgacg accatctgg agcacctag taccatcag atccgacca ttttaataa ctcatgggt ggacagatc aggaaacag catcatgga tccaagaca tggcatttt tgtaccata aaaaacgca actacaacg ttattgcag tggactggt caatcgatg ccgacaaaa actacatct actcatcat cagcaactc aatctgttg gtatcatta ttggtgctt tcctaatcc aagtctcaa aacatgtgg aaaactttt tcgaaaaag ttaacttaa tcgggcatt ctttaggcg cccaagttg taggatttg caggaaagt acgtggaaa actccacag attccaaac ttgatagaa tcacgggtt tagacgctg caagccccc ttttcgaaa ccccaattt tgaggcccc ctgaactgc gattggctg acagcgatg ccaattttg ttgatctta ttcacacag ctttaggag ttggtgtga caacaatta gggaaattt tttcccaaa atttgatta acgaagact cctcttttt tgagccaac tcttcaacg tttcgatca acaacatcg cagctgttg ctaatggtt tttccttca tcaacatcc caacagata acgttaaat tccttttat tcgaacgca ctagcaatc aaactatca ctctcaacc atgacgacc ctctggagc acttagtac catcagatc cgaccattt taataactc atgggtgga cagatcagg gaacagcat catggatcc aagacatgg catttttgt accataaaa aaggcaact acaacgtta ttgcagtgg attggtcaa tcgatgctg acagaaact acatctact catcatcag caacccaat ctgttggta tcattattg gtgctttcc taatccaag tctcaaaaa aagtggaca actttttcg aaaaagttc acctaattg ggcattctt taggtgccc aagttgtag gatttgcag gaaagtacg tggaaaact ccacagatt ccaaacttg atagaatca caggtctag acgctgcaa gcccccttt ttgaaaccc ccattttga gaccccctg aactgcgat tggctgaca ccgatgcca gttttgttg atcttattc acacagctt taggggttg ggtatattg gggcttttg gaaccgccg acttctatg tcaatgggg gtattattc aattaaatt gtacagacg acttgaccg atattgcgt cttgtaatc accagagct cacatattt actattcgg agacaatat tagagcgaa aaaagtacg aagcaacca aatgtgagg atgctgtaa ggtttaatt tggggttgt gtgacgata acgacaatg cttatatgg gcgatgaga ttgaaatgt tgaaaactt taacgtgtc gctcactca cacatcgat catcaggtt atctaaccc tgctttaca aatccaaaa tgtcgcaaa atacagtcc ctgttgaga cttcaattc gtggaaaac tcagttcag agctacaaa ctacccact tggaggtca tcaacgagt catacatgc acaacgtgc ctaaaggcg ccgagacac atttcaaag ttgtggtgg tctctgaga aattttcag acctgcctt taatcaaga gacacagat tggtaaacg acattttaa aggaagaat tgcaaagtg gagtccatg ctctctcga ttgttgcca aaacccctt cacagtgga gcagcagtg agcaaataa tcgaaccca gtcctaact gtaggggcg gcttcggca aatgattgt agtacttga taacttaat tag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
