Status
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Gene information
General information
TC031354
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011285,TC011286
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2117833..2124427
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031354
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08292

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva segment central constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Constriction of abdomen segment is major phenotype but with this phenotype there is also head & thorax appendages shape irregular & larvae segements are randomly partially not present in all three tagmata.
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031354 RA
      MCPLVFLFL ISVTTIREI FSQNLINED SSFLSQLFN 
      VSINNIAAV ANGFSFINI PTDNVKFFL FERTSNQTI 
      TLNRDDHLE HLVPSDPTI LITHGWTDQ ETASWIQDM 
      AFLYHKKRN YNVIAVDWS IDADKNYIY SSSATQSVG 
      IIIGAFLIQ VSKHVENFF EKVNLIGHS LGAQVVGFA 
      GKYVENSTD SKLDRITGL DAASPLFET PILRPPELR 
      LADSDANFV DLIHTALGV GVTTIREIF SQNLINEDS 
      SFLSQLFNV SINNIAAVA NGFSFINIP TDNVKFLLF 
      ERTSNQTIT LNHDDPLEH LVPSDPTIL ITHGWTDQG 
      TASWIQDMA FLYHKKGNY NVIAVDWSI DADRNYIYS 
      SSATQSVGI IIGAFLIQV SKKVDNFFE KVHLIGHSL 
      GAQVVGFAG KYVENSTDS KLDRITGLD AASPLFETP 
      ILRPPELRL ADTDASFVD LIHTALGVG YIGAFGTAD 
      FYVNGGIIQ LNCTDDLTD IASCNHQSS HIYYSETIL 
      ERKKYEATK CEDAVRFNL GLCDDNDNA YMGDEIEML 
      KTLTCRSLT HRSSGYLTL LYKSKMSQN TVPVETSIR 
      GKLSSELQT THLEVINES YMHNVPKGA ETHFKVVVV 
      SEKFSDLPL IKRHRLVND ILKEELQSG VHALSIVAK 
      TPSQWSSSE QIIEPSPNC RGGFGK
    CDS sequence
    • >TC031354 RA
      atgtgtcca ctggtattt ctcttcttg attagtgtg 
      acaacaatt agggaaatt ttttcccaa aatttgatt 
      aacgaagac tcctctttc ttgagccaa ctcttcaac 
      gtttcgatc aacaacatc gcagctgtt gctaatggt 
      ttttccttc atcaacatc ccaacagat aacgttaaa 
      ttcttttta ttcgaacgc actagcaat caaactatc 
      actctcaac cgtgacgac catctggag cacctagta 
      ccatcagat ccgaccatt ttaataact catgggtgg 
      acagatcag gaaacagca tcatggatc caagacatg 
      gcatttttg taccataaa aaacgcaac tacaacgtt 
      attgcagtg gactggtca atcgatgcc gacaaaaac 
      tacatctac tcatcatca gcaactcaa tctgttggt 
      atcattatt ggtgctttc ctaatccaa gtctcaaaa 
      catgtggaa aactttttc gaaaaagtt aacttaatc 
      gggcattct ttaggcgcc caagttgta ggatttgca 
      ggaaagtac gtggaaaac tccacagat tccaaactt 
      gatagaatc acgggttta gacgctgca agccccctt 
      ttcgaaacc ccaattttg aggccccct gaactgcga 
      ttggctgac agcgatgcc aattttgtt gatcttatt 
      cacacagct ttaggagtt ggtgtgaca acaattagg 
      gaaattttt tcccaaaat ttgattaac gaagactcc 
      tcttttttg agccaactc ttcaacgtt tcgatcaac 
      aacatcgca gctgttgct aatggtttt tccttcatc 
      aacatccca acagataac gttaaattc cttttattc 
      gaacgcact agcaatcaa actatcact ctcaaccat 
      gacgaccct ctggagcac ttagtacca tcagatccg 
      accatttta ataactcat gggtggaca gatcaggga 
      acagcatca tggatccaa gacatggca tttttgtac 
      cataaaaaa ggcaactac aacgttatt gcagtggat 
      tggtcaatc gatgctgac agaaactac atctactca 
      tcatcagca acccaatct gttggtatc attattggt 
      gctttccta atccaagtc tcaaaaaaa gtggacaac 
      tttttcgaa aaagttcac ctaattggg cattcttta 
      ggtgcccaa gttgtagga tttgcagga aagtacgtg 
      gaaaactcc acagattcc aaacttgat agaatcaca 
      ggtctagac gctgcaagc ccccttttt gaaaccccc 
      attttgaga ccccctgaa ctgcgattg gctgacacc 
      gatgccagt tttgttgat cttattcac acagcttta 
      ggggttggg tatattggg gcttttgga accgccgac 
      ttctatgtc aatgggggt attattcaa ttaaattgt 
      acagacgac ttgaccgat attgcgtct tgtaatcac 
      cagagctca catatttac tattcggag acaatatta 
      gagcgaaaa aagtacgaa gcaaccaaa tgtgaggat 
      gctgtaagg tttaatttg gggttgtgt gacgataac 
      gacaatgct tatatgggc gatgagatt gaaatgttg 
      aaaacttta acgtgtcgc tcactcaca catcgatca 
      tcaggttat ctaaccctg ctttacaaa tccaaaatg 
      tcgcaaaat acagtccct gttgagact tcaattcgt 
      ggaaaactc agttcagag ctacaaact acccacttg 
      gaggtcatc aacgagtca tacatgcac aacgtgcct 
      aaaggcgcc gagacacat ttcaaagtt gtggtggtc 
      tctgagaaa ttttcagac ctgccttta atcaagaga 
      cacagattg gtaaacgac attttaaag gaagaattg 
      caaagtgga gtccatgct ctctcgatt gttgccaaa 
      accccttca cagtggagc agcagtgag caaataatc 
      gaacccagt cctaactgt aggggcggc ttcggcaaa 
      tga
    mRNA sequence
    • >TC031354 RA
      gatgatatc tatgtttcg aatatttgt cagcggttt 
      aattattac tacgcatgg aaaatttaa aatatattt 
      taaccataa atcagaata tataaatta aaataaatt 
      tttgccatt ttcgatgcg aattttatt tagttacct 
      agaaaaagt tatgcaaag taaatcaaa acatgtaat 
      aatatcgag aaacgtgca caatgtgtc cactggtat 
      ttctcttct tgattagtg tgacaacaa ttagggaaa 
      ttttttccc aaaatttga ttaacgaag actcctctt 
      tcttgagcc aactcttca acgtttcga tcaacaaca 
      tcgcagctg ttgctaatg gtttttcct tcatcaaca 
      tcccaacag ataacgtta aattctttt tattcgaac 
      gcactagca atcaaacta tcactctca accgtgacg 
      accatctgg agcacctag taccatcag atccgacca 
      ttttaataa ctcatgggt ggacagatc aggaaacag 
      catcatgga tccaagaca tggcatttt tgtaccata 
      aaaaacgca actacaacg ttattgcag tggactggt 
      caatcgatg ccgacaaaa actacatct actcatcat 
      cagcaactc aatctgttg gtatcatta ttggtgctt 
      tcctaatcc aagtctcaa aacatgtgg aaaactttt 
      tcgaaaaag ttaacttaa tcgggcatt ctttaggcg 
      cccaagttg taggatttg caggaaagt acgtggaaa 
      actccacag attccaaac ttgatagaa tcacgggtt 
      tagacgctg caagccccc ttttcgaaa ccccaattt 
      tgaggcccc ctgaactgc gattggctg acagcgatg 
      ccaattttg ttgatctta ttcacacag ctttaggag 
      ttggtgtga caacaatta gggaaattt tttcccaaa 
      atttgatta acgaagact cctcttttt tgagccaac 
      tcttcaacg tttcgatca acaacatcg cagctgttg 
      ctaatggtt tttccttca tcaacatcc caacagata 
      acgttaaat tccttttat tcgaacgca ctagcaatc 
      aaactatca ctctcaacc atgacgacc ctctggagc 
      acttagtac catcagatc cgaccattt taataactc 
      atgggtgga cagatcagg gaacagcat catggatcc 
      aagacatgg catttttgt accataaaa aaggcaact 
      acaacgtta ttgcagtgg attggtcaa tcgatgctg 
      acagaaact acatctact catcatcag caacccaat 
      ctgttggta tcattattg gtgctttcc taatccaag 
      tctcaaaaa aagtggaca actttttcg aaaaagttc 
      acctaattg ggcattctt taggtgccc aagttgtag 
      gatttgcag gaaagtacg tggaaaact ccacagatt 
      ccaaacttg atagaatca caggtctag acgctgcaa 
      gcccccttt ttgaaaccc ccattttga gaccccctg 
      aactgcgat tggctgaca ccgatgcca gttttgttg 
      atcttattc acacagctt taggggttg ggtatattg 
      gggcttttg gaaccgccg acttctatg tcaatgggg 
      gtattattc aattaaatt gtacagacg acttgaccg 
      atattgcgt cttgtaatc accagagct cacatattt 
      actattcgg agacaatat tagagcgaa aaaagtacg 
      aagcaacca aatgtgagg atgctgtaa ggtttaatt 
      tggggttgt gtgacgata acgacaatg cttatatgg 
      gcgatgaga ttgaaatgt tgaaaactt taacgtgtc 
      gctcactca cacatcgat catcaggtt atctaaccc 
      tgctttaca aatccaaaa tgtcgcaaa atacagtcc 
      ctgttgaga cttcaattc gtggaaaac tcagttcag 
      agctacaaa ctacccact tggaggtca tcaacgagt 
      catacatgc acaacgtgc ctaaaggcg ccgagacac 
      atttcaaag ttgtggtgg tctctgaga aattttcag 
      acctgcctt taatcaaga gacacagat tggtaaacg 
      acattttaa aggaagaat tgcaaagtg gagtccatg 
      ctctctcga ttgttgcca aaacccctt cacagtgga 
      gcagcagtg agcaaataa tcgaaccca gtcctaact 
      gtaggggcg gcttcggca aatgattgt agtacttga 
      taacttaat tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY