Status
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Gene information
General information
TC033127
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013482,TC013483
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8992005..9013619
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033127
    iBeetle screen iB_08291

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033127 RA
      MGNKLSCSC APLIRKAYR YEDSPWQTS RRRDGHLLR 
      LWAEVFHVS ASGAGTVKW QQVSEDLVP VNITCIQDS 
      PECVFHITA YNSQVDKIL DVRLVQPGT RIGQASECF 
      VYWKDPMTN DTWGLNFTS PIDAKQFRE CCSPSFKFS 
      RKASSSYSL KLEPPNKQK FKTKRKPLS TPASPSRTR 
      EPQCTCMTA EQYARLRAQ DPRFRGSST LPRAQTRAT 
      DTEGRADKV TAATSSTSL YDNVGATQT GSTQGDVCD 
      KNMLALGTK GTQREGSQT RQMRDRGVQ QQSQETSTM 
      TSNTATGPK TVTASVGTQ NSSQIVQTE TQSQTQTEA 
      TKTEGTQAG GTLQMNTKT SSTSTRPPL RDNAHHVLT 
      NTKSVDYSE IDMREMDLR AAHHHNLLN NNISNRRSK 
      SKSTEDMNR ENSISLDSN TLKRMLKPM PSAESPVTS 
      PEMGRRRYN YYNNHHHRH PNNNGRHSE PESGHPHPR 
      SAMAQNSRF SGSRSSHEI GRGYPGRGL YLELERGAG 
      GDLSPPSDN VLFDNQCYA TTPSSSNGN SDPEQGGHH 
      YGRQRHMHH QNANNTPTP GSPTSRLLL EYEMHLRNT 
      LAKGMDAES YSLHTFEAL LTQSMENLE FAESLPGST 
      QRSPYPSRK RPSSSASIN KSSTLPLSH RLGAVARDR 
      ERDGYYSDR NELLREREK ERERGYLSD HNSSFSSRC 
      ASCIGESSR AQWFRHSDG WRSGSSNFG SGQTLPQGH 
      KRSPWDSLP SLRQDSSLN DSGYKSNRA DSFEQRGMF 
      DRQDSLRSD YMSDRESSR YGIVQQASI ESADSRLCY 
      LTSSELGVV GAHGSNMWG VRDFVTSFL KSLVLNGGG 
      RIVKTVPSL LETPSYVCA NFIGLETMV ESNSCSRKC 
      GRVCNGETS PEKSLMSKR RRHKTVHFG DNLLLQVCT 
      NANLQSHVS YSNMEPNVQ QLFSFIETV LSAWVAEEG 
      LTSQGEVSE MDEKELRKR RNKKHGAKV KRLDIRRLV 
      GEVSKLDGR FLGNLRYRH MHWKGNPNS EGCNELFLR 
      KISDDDRMS LTTAVSDED DGESVMNSP YKAKQTGTA 
      AASFNCTGA VRKAG
    • >TC033127 RB
      MGNKLSCSC APLIRKAYR YEDSPWQTS RRRDGHLLR 
      LWAEVFHVS ASGAGTVKW QQVSEDLVP VNITCIQDS 
      PECVFHITA YNSQVDKIL DVRLVQPGT RIGQASECF 
      VYWKDPMTN DTWGLNFTS PIDAKQFRE CCSPSFKFS 
      RKASSSYSL KLEPPNKQK FKTKRKPLS TPASPSRTR 
      EPQCTCMTA EQYARLRAQ DPRFRGSST LPRAQTRAT 
      DTEGRADKV TAATSSTSL YDNVGATQT GSTQGDVCD 
      KNMLALGTK GTQREGSQT RQMRDRGVQ QQSQETSTM 
      TSNTATGPK TVTASVGTQ NSSQIVQTE TQSQTQTEA 
      TKTEGTQAG GTLQMNTKT SSTSTRPPL RDNAHHVLT 
      NTKSVDYSE IDMREMDLR AAHHHNLLN NNISNRRSK 
      SKSTEDMNR ENSISLDSN TLKRMLKPM PSAESPVTS 
      PEMGRRRYN YYNNHHHRH PNNNGRHSE PESGHPHPR 
      SAMAQNSRF SGSSFNYRS SHEIGRGYP GRGLYLELE 
      RGAGGDLSP PSDNVLFDN QCYATTPSS SNGNSDPEQ 
      GGHHYGRQR HMHHQNANN TPTPGSPTS RLLLEYEMH 
      LRNTLAKGM DAESYSLHT FEALLTQSM ENLEFAESL 
      PGSTQRSPY PSRKRPSSS ASINKSSTL PLSHRLGAV 
      ARDRERDGY YSDRNELLR EREKERERG YLSDHNSSF 
      SSRCASCIG ESSRAQWFR HSDGWRSGS SNFGSGQTL 
      PQGHKRSPW DSLPSLRQD SSLNDSGYK SNRADSFEQ 
      RGMFDRQDS LRSDYMSDR ESSRYGIVQ QASIESADS 
      RLCYLTSSE LGVVGAHGS NMWGVRDFV TSFLKSLVL 
      NGGGRIVKT VPSLLETPS YVCANFIGL ETMVESNSC 
      SRKCGRVCN GETSPEKSL MSKRRRHKT VHFGDNLLL 
      QVCTNANLQ SHVSYSNME PNVQQLFSF IETVLSAWV 
      AEEGLTSQG EVSEMDEKE LRKRRNKKH GAKVKRLDI 
      RRLVGEVSK LDGRFLGNL RYRHMHWKG NPNSEGCNE 
      LFLRKISDD DRMSLTTAV SDEDDGESV MNSPYKAKQ 
      TGTAAASFN CTGAVRKAG
    CDS sequence
    • >TC033127 RA
      atgggcaac aaactcagc tgctcgtgc gcccctctc 
      atcaggaag gcgtaccga tacgaggac tcgccgtgg 
      cagacgtcc aggcgtcgc gacggacac ctcctcaga 
      ttatgggcg gaggtgttc cacgtgtct gcgagtggc 
      gctggtacc gtcaagtgg cagcaagtt agtgaggat 
      ctcgtgcct gttaacatc acctgcatt caggactcg 
      ccggaatgc gtatttcac atcacggcc tacaactcg 
      caggtcgac aagattctg gatgtgcgg ctggtccag 
      cctgggacg aggataggg caagcgtcg gagtgcttc 
      gtgtactgg aaagatccc atgaccaac gacacgtgg 
      ggattgaat tttacctcg cccatcgat gcaaaacag 
      ttcagagaa tgttgttcg ccgtcgttc aaattctcc 
      cggaaggcg tcgtcttcc tactcgctg aagctggag 
      ccgccgaac aaacaaaaa ttcaaaacg aaacgaaag 
      cctttgagc actccggcg tcgccgagt cgcactcgc 
      gagccgcaa tgcacctgc atgacagct gagcaatac 
      gcaagactg agggcacag gacccacga tttcgagga 
      tcttcgact ttgccgcgg gcgcagacc cgcgccacc 
      gacacggag ggaagagcc gacaaagtg accgccgcc 
      acttcttcc actagtctt tacgataac gttggtgct 
      acacaaact ggttcaaca caaggtgat gtttgtgat 
      aaaaatatg cttgcttta ggtactaaa gggacacaa 
      agagagggc agccagacc cggcaaatg cgcgaccga 
      ggggtgcaa cagcagtcg caggagacc agcacgatg 
      acatccaac accgcgaca ggaccaaaa accgtgacc 
      gcctcggtg ggaacgcaa aacagctcg caaatcgta 
      caaaccgaa acccagtct caaacccag accgaagcg 
      acgaagact gaggggacg caagcggga ggcacgctc 
      cagatgaac acgaagacc tcctccacg tccacgagg 
      cctccgctg agggacaac gcccaccac gtgctcact 
      aataccaag tcggtggac tactcggag atcgacatg 
      agggagatg gacctgcgg gcggcccac caccacaac 
      ctcctcaac aacaatatc agcaatagg aggagcaag 
      tcgaagagc acggaggat atgaacagg gagaacagc 
      ataagtttg gacagcaac acgttgaag aggatgctc 
      aagcccatg cccagtgcc gaaagcccc gtcacatcg 
      ccggaaatg ggcagacgg aggtataac tattacaat 
      aatcatcac cacaggcat ccgaacaac aatgggagg 
      cattcggaa ccggagtcg gggcatccg catcccagg 
      tccgccatg gcacaaaat tcaaggttc tcgggctct 
      agatcatcg cacgagatc ggccgggga taccccggc 
      cgcggcctg tacctggaa cttgagcgc ggagctggc 
      ggtgactta tctccacct tccgacaac gttcttttc 
      gacaaccaa tgctacgcc acaactccg agctcaagt 
      aacggaaat tcggacccg gaacaaggc gggcaccat 
      tatggccgc cagaggcac atgcaccac cagaacgcc 
      aataacacc ccgacccct ggctccccc acaagccgc 
      cttctgctg gaatacgaa atgcacctc cggaacact 
      ctagccaaa ggcatggac gcggagagc tacagtctg 
      cacactttt gaagccctt ctgacccag agtatggaa 
      aatttagaa ttcgccgaa agccttccc ggatcgacc 
      caacgcagc ccttaccct tcccgaaaa cgaccttca 
      tccagcgct tccatcaac aaatcatca actttacca 
      ctgtcccat cggctgggc gcggtggcc cgcgaccgc 
      gaacgcgac ggctactac agcgaccgc aacgagctg 
      ctgcgcgaa agggaaaag gaacgagaa cggggatat 
      ttgagcgac cacaactcc agtttcagt tcgaggtgt 
      gcttcgtgt ataggggag tcgagtcgg gcgcagtgg 
      ttccggcat tctgatggc tggaggtcg ggcagctcg 
      aactttggc tcggggcag acgctgccg caagggcac 
      aagaggtcg ccctgggat tcgttacct tcgctgagg 
      caggacagc agtttgaat gatagcgga tataagagc 
      aatcgggcc gacagcttt gagcaacgg gggatgttc 
      gatcgccag gacagtctt cgctcggat tacatgagt 
      gatagagag tcttccaga tatggaatc gttcagcag 
      gcttccatc gagagcgcc gactcgagg ctgtgctat 
      ctcacgtcg tcagagctg ggagtagtt ggggcacat 
      gggagcaac atgtggggg gtgagggat tttgtaacg 
      agtttcctc aagagtttg gttttaaat gggggtggg 
      cgaattgtc aaaacagtg ccttcattg ttggaaaca 
      ccaagttat gtttgcgca aattttatc ggattagaa 
      acgatggtg gagtcgaac tcgtgctcg aggaaatgc 
      ggccgagtg tgtaacggg gaaacctcg ccggagaag 
      tcgctcatg tccaagcgc cggaggcac aagacggtg 
      cacttcggc gacaatctg ctcctacaa gtgtgcacc 
      aacgccaat ctgcagtcg cacgtttcc tactcgaac 
      atggagccc aacgtccag cagctcttc agcttcatt 
      gagacggtg ttgagtgcg tgggtggcc gaagaaggg 
      ctcacctcg cagggcgaa gtgagcgaa atggacgag 
      aaggaactg cgaaaacgg agaaacaag aaacacgga 
      gccaaagtc aaaagactt gacatcagg cgattggtt 
      ggggaagtg tcgaagtta gatggacga tttttggga 
      aatctgaga tacagacac atgcactgg aagggaaac 
      cccaactcg gagggctgt aacgagctc tttctcagg 
      aagatatcc gacgatgac cgcatgtcg ttaacgacg 
      gcggtgtcc gacgaagat gatggcgag agcgttatg 
      aactcgcct tataaagcg aaacagacg ggaaccgcc 
      gctgcctct ttcaactgc accggagcc gtccggaaa 
      gccgggtaa
    • >TC033127 RB
      atgggcaac aaactcagc tgctcgtgc gcccctctc 
      atcaggaag gcgtaccga tacgaggac tcgccgtgg 
      cagacgtcc aggcgtcgc gacggacac ctcctcaga 
      ttatgggcg gaggtgttc cacgtgtct gcgagtggc 
      gctggtacc gtcaagtgg cagcaagtt agtgaggat 
      ctcgtgcct gttaacatc acctgcatt caggactcg 
      ccggaatgc gtatttcac atcacggcc tacaactcg 
      caggtcgac aagattctg gatgtgcgg ctggtccag 
      cctgggacg aggataggg caagcgtcg gagtgcttc 
      gtgtactgg aaagatccc atgaccaac gacacgtgg 
      ggattgaat tttacctcg cccatcgat gcaaaacag 
      ttcagagaa tgttgttcg ccgtcgttc aaattctcc 
      cggaaggcg tcgtcttcc tactcgctg aagctggag 
      ccgccgaac aaacaaaaa ttcaaaacg aaacgaaag 
      cctttgagc actccggcg tcgccgagt cgcactcgc 
      gagccgcaa tgcacctgc atgacagct gagcaatac 
      gcaagactg agggcacag gacccacga tttcgagga 
      tcttcgact ttgccgcgg gcgcagacc cgcgccacc 
      gacacggag ggaagagcc gacaaagtg accgccgcc 
      acttcttcc actagtctt tacgataac gttggtgct 
      acacaaact ggttcaaca caaggtgat gtttgtgat 
      aaaaatatg cttgcttta ggtactaaa gggacacaa 
      agagagggc agccagacc cggcaaatg cgcgaccga 
      ggggtgcaa cagcagtcg caggagacc agcacgatg 
      acatccaac accgcgaca ggaccaaaa accgtgacc 
      gcctcggtg ggaacgcaa aacagctcg caaatcgta 
      caaaccgaa acccagtct caaacccag accgaagcg 
      acgaagact gaggggacg caagcggga ggcacgctc 
      cagatgaac acgaagacc tcctccacg tccacgagg 
      cctccgctg agggacaac gcccaccac gtgctcact 
      aataccaag tcggtggac tactcggag atcgacatg 
      agggagatg gacctgcgg gcggcccac caccacaac 
      ctcctcaac aacaatatc agcaatagg aggagcaag 
      tcgaagagc acggaggat atgaacagg gagaacagc 
      ataagtttg gacagcaac acgttgaag aggatgctc 
      aagcccatg cccagtgcc gaaagcccc gtcacatcg 
      ccggaaatg ggcagacgg aggtataac tattacaat 
      aatcatcac cacaggcat ccgaacaac aatgggagg 
      cattcggaa ccggagtcg gggcatccg catcccagg 
      tccgccatg gcacaaaat tcaaggttc tcgggctct 
      agcttcaat tacagatca tcgcacgag atcggccgg 
      ggatacccc ggccgcggc ctgtacctg gaacttgag 
      cgcggagct ggcggtgac ttatctcca ccttccgac 
      aacgttctt ttcgacaac caatgctac gccacaact 
      ccgagctca agtaacgga aattcggac ccggaacaa 
      ggcgggcac cattatggc cgccagagg cacatgcac 
      caccagaac gccaataac accccgacc cctggctcc 
      cccacaagc cgccttctg ctggaatac gaaatgcac 
      ctccggaac actctagcc aaaggcatg gacgcggag 
      agctacagt ctgcacact tttgaagcc cttctgacc 
      cagagtatg gaaaattta gaattcgcc gaaagcctt 
      cccggatcg acccaacgc agcccttac ccttcccga 
      aaacgacct tcatccagc gcttccatc aacaaatca 
      tcaacttta ccactgtcc catcggctg ggcgcggtg 
      gcccgcgac cgcgaacgc gacggctac tacagcgac 
      cgcaacgag ctgctgcgc gaaagggaa aaggaacga 
      gaacgggga tatttgagc gaccacaac tccagtttc 
      agttcgagg tgtgcttcg tgtataggg gagtcgagt 
      cgggcgcag tggttccgg cattctgat ggctggagg 
      tcgggcagc tcgaacttt ggctcgggg cagacgctg 
      ccgcaaggg cacaagagg tcgccctgg gattcgtta 
      ccttcgctg aggcaggac agcagtttg aatgatagc 
      ggatataag agcaatcgg gccgacagc tttgagcaa 
      cgggggatg ttcgatcgc caggacagt cttcgctcg 
      gattacatg agtgataga gagtcttcc agatatgga 
      atcgttcag caggcttcc atcgagagc gccgactcg 
      aggctgtgc tatctcacg tcgtcagag ctgggagta 
      gttggggca catgggagc aacatgtgg ggggtgagg 
      gattttgta acgagtttc ctcaagagt ttggtttta 
      aatgggggt gggcgaatt gtcaaaaca gtgccttca 
      ttgttggaa acaccaagt tatgtttgc gcaaatttt 
      atcggatta gaaacgatg gtggagtcg aactcgtgc 
      tcgaggaaa tgcggccga gtgtgtaac ggggaaacc 
      tcgccggag aagtcgctc atgtccaag cgccggagg 
      cacaagacg gtgcacttc ggcgacaat ctgctccta 
      caagtgtgc accaacgcc aatctgcag tcgcacgtt 
      tcctactcg aacatggag cccaacgtc cagcagctc 
      ttcagcttc attgagacg gtgttgagt gcgtgggtg 
      gccgaagaa gggctcacc tcgcagggc gaagtgagc 
      gaaatggac gagaaggaa ctgcgaaaa cggagaaac 
      aagaaacac ggagccaaa gtcaaaaga cttgacatc 
      aggcgattg gttggggaa gtgtcgaag ttagatgga 
      cgatttttg ggaaatctg agatacaga cacatgcac 
      tggaaggga aaccccaac tcggagggc tgtaacgag 
      ctctttctc aggaagata tccgacgat gaccgcatg 
      tcgttaacg acggcggtg tccgacgaa gatgatggc 
      gagagcgtt atgaactcg ccttataaa gcgaaacag 
      acgggaacc gccgctgcc tctttcaac tgcaccgga 
      gccgtccgg aaagccggg taa
    mRNA sequence
    • >TC033127 RA
      agctttttc cggggggcc accggacca tatcgcagt 
      gccaaaggg tgcggagca cgtgacgcc cgccccaca 
      tgtatcaac ccttgtgcc acaccattt agtgtcaaa 
      tttttatac cggactttc aagatggga gcaaaaggg 
      agcgtcgaa gccgccaag atcaatgca caaaactgg 
      aataacctg gagcataaa cacaaaccc gcacaccag 
      cggtctcag tctctgaac cgtggcgtt cgtggtcag 
      tggggctcc cgagggccc gagttcgct cccgacatg 
      tggtgacag cggggccag tgccagtgc ccagcggcg 
      gcgatgggc aacaaactc agctgctcg tgcgcccct 
      ctcatcagg aaggcgtac cgatacgag gactcgccg 
      tggcagacg tccaggcgt cgcgacgga cacctcctc 
      agattatgg gcggaggtg ttccacgtg tctgcgagt 
      ggcgctggt accgtcaag tggcagcaa gttagtgag 
      gatctcgtg cctgttaac atcacctgc attcaggac 
      tcgccggaa tgcgtattt cacatcacg gcctacaac 
      tcgcaggtc gacaagatt ctggatgtg cggctggtc 
      cagcctggg acgaggata gggcaagcg tcggagtgc 
      ttcgtgtac tggaaagat cccatgacc aacgacacg 
      tggggattg aattttacc tcgcccatc gatgcaaaa 
      cagttcaga gaatgttgt tcgccgtcg ttcaaattc 
      tcccggaag gcgtcgtct tcctactcg ctgaagctg 
      gagccgccg aacaaacaa aaattcaaa acgaaacga 
      aagcctttg agcactccg gcgtcgccg agtcgcact 
      cgcgagccg caatgcacc tgcatgaca gctgagcaa 
      tacgcaaga ctgagggca caggaccca cgatttcga 
      ggatcttcg actttgccg cgggcgcag acccgcgcc 
      accgacacg gagggaaga gccgacaaa gtgaccgcc 
      gccacttct tccactagt ctttacgat aacgttggt 
      gctacacaa actggttca acacaaggt gatgtttgt 
      gataaaaat atgcttgct ttaggtact aaagggaca 
      caaagagag ggcagccag acccggcaa atgcgcgac 
      cgaggggtg caacagcag tcgcaggag accagcacg 
      atgacatcc aacaccgcg acaggacca aaaaccgtg 
      accgcctcg gtgggaacg caaaacagc tcgcaaatc 
      gtacaaacc gaaacccag tctcaaacc cagaccgaa 
      gcgacgaag actgagggg acgcaagcg ggaggcacg 
      ctccagatg aacacgaag acctcctcc acgtccacg 
      aggcctccg ctgagggac aacgcccac cacgtgctc 
      actaatacc aagtcggtg gactactcg gagatcgac 
      atgagggag atggacctg cgggcggcc caccaccac 
      aacctcctc aacaacaat atcagcaat aggaggagc 
      aagtcgaag agcacggag gatatgaac agggagaac 
      agcataagt ttggacagc aacacgttg aagaggatg 
      ctcaagccc atgcccagt gccgaaagc cccgtcaca 
      tcgccggaa atgggcaga cggaggtat aactattac 
      aataatcat caccacagg catccgaac aacaatggg 
      aggcattcg gaaccggag tcggggcat ccgcatccc 
      aggtccgcc atggcacaa aattcaagg ttctcgggc 
      tctagatca tcgcacgag atcggccgg ggatacccc 
      ggccgcggc ctgtacctg gaacttgag cgcggagct 
      ggcggtgac ttatctcca ccttccgac aacgttctt 
      ttcgacaac caatgctac gccacaact ccgagctca 
      agtaacgga aattcggac ccggaacaa ggcgggcac 
      cattatggc cgccagagg cacatgcac caccagaac 
      gccaataac accccgacc cctggctcc cccacaagc 
      cgccttctg ctggaatac gaaatgcac ctccggaac 
      actctagcc aaaggcatg gacgcggag agctacagt 
      ctgcacact tttgaagcc cttctgacc cagagtatg 
      gaaaattta gaattcgcc gaaagcctt cccggatcg 
      acccaacgc agcccttac ccttcccga aaacgacct 
      tcatccagc gcttccatc aacaaatca tcaacttta 
      ccactgtcc catcggctg ggcgcggtg gcccgcgac 
      cgcgaacgc gacggctac tacagcgac cgcaacgag 
      ctgctgcgc gaaagggaa aaggaacga gaacgggga 
      tatttgagc gaccacaac tccagtttc agttcgagg 
      tgtgcttcg tgtataggg gagtcgagt cgggcgcag 
      tggttccgg cattctgat ggctggagg tcgggcagc 
      tcgaacttt ggctcgggg cagacgctg ccgcaaggg 
      cacaagagg tcgccctgg gattcgtta ccttcgctg 
      aggcaggac agcagtttg aatgatagc ggatataag 
      agcaatcgg gccgacagc tttgagcaa cgggggatg 
      ttcgatcgc caggacagt cttcgctcg gattacatg 
      agtgataga gagtcttcc agatatgga atcgttcag 
      caggcttcc atcgagagc gccgactcg aggctgtgc 
      tatctcacg tcgtcagag ctgggagta gttggggca 
      catgggagc aacatgtgg ggggtgagg gattttgta 
      acgagtttc ctcaagagt ttggtttta aatgggggt 
      gggcgaatt gtcaaaaca gtgccttca ttgttggaa 
      acaccaagt tatgtttgc gcaaatttt atcggatta 
      gaaacgatg gtggagtcg aactcgtgc tcgaggaaa 
      tgcggccga gtgtgtaac ggggaaacc tcgccggag 
      aagtcgctc atgtccaag cgccggagg cacaagacg 
      gtgcacttc ggcgacaat ctgctccta caagtgtgc 
      accaacgcc aatctgcag tcgcacgtt tcctactcg 
      aacatggag cccaacgtc cagcagctc ttcagcttc 
      attgagacg gtgttgagt gcgtgggtg gccgaagaa 
      gggctcacc tcgcagggc gaagtgagc gaaatggac 
      gagaaggaa ctgcgaaaa cggagaaac aagaaacac 
      ggagccaaa gtcaaaaga cttgacatc aggcgattg 
      gttggggaa gtgtcgaag ttagatgga cgatttttg 
      ggaaatctg agatacaga cacatgcac tggaaggga 
      aaccccaac tcggagggc tgtaacgag ctctttctc 
      aggaagata tccgacgat gaccgcatg tcgttaacg 
      acggcggtg tccgacgaa gatgatggc gagagcgtt 
      atgaactcg ccttataaa gcgaaacag acgggaacc 
      gccgctgcc tctttcaac tgcaccgga gccgtccgg 
      aaagccggg taagtactc cggagccgc ttattagca 
      gtgttacgt gaggtggaa ggcaataac tactgatac 
      aataaatac acaaagtca atgatg
    • >TC033127 RB
      agctttttc cggggggcc accggacca tatcgcagt 
      gccaaaggg tgcggagca cgtgacgcc cgccccaca 
      tgtatcaac ccttgtgcc acaccattt agtgtcaaa 
      tttttatac cggactttc aagatggga gcaaaaggg 
      agcgtcgaa gccgccaag atcaatgca caaaactgg 
      aataacctg gagcataaa cacaaaccc gcacaccag 
      cggtctcag tctctgaac cgtggcgtt cgtggtcag 
      tggggctcc cgagggccc gagttcgct cccgacatg 
      tggtgacag cggggccag tgccagtgc ccagcggcg 
      gcgatgggc aacaaactc agctgctcg tgcgcccct 
      ctcatcagg aaggcgtac cgatacgag gactcgccg 
      tggcagacg tccaggcgt cgcgacgga cacctcctc 
      agattatgg gcggaggtg ttccacgtg tctgcgagt 
      ggcgctggt accgtcaag tggcagcaa gttagtgag 
      gatctcgtg cctgttaac atcacctgc attcaggac 
      tcgccggaa tgcgtattt cacatcacg gcctacaac 
      tcgcaggtc gacaagatt ctggatgtg cggctggtc 
      cagcctggg acgaggata gggcaagcg tcggagtgc 
      ttcgtgtac tggaaagat cccatgacc aacgacacg 
      tggggattg aattttacc tcgcccatc gatgcaaaa 
      cagttcaga gaatgttgt tcgccgtcg ttcaaattc 
      tcccggaag gcgtcgtct tcctactcg ctgaagctg 
      gagccgccg aacaaacaa aaattcaaa acgaaacga 
      aagcctttg agcactccg gcgtcgccg agtcgcact 
      cgcgagccg caatgcacc tgcatgaca gctgagcaa 
      tacgcaaga ctgagggca caggaccca cgatttcga 
      ggatcttcg actttgccg cgggcgcag acccgcgcc 
      accgacacg gagggaaga gccgacaaa gtgaccgcc 
      gccacttct tccactagt ctttacgat aacgttggt 
      gctacacaa actggttca acacaaggt gatgtttgt 
      gataaaaat atgcttgct ttaggtact aaagggaca 
      caaagagag ggcagccag acccggcaa atgcgcgac 
      cgaggggtg caacagcag tcgcaggag accagcacg 
      atgacatcc aacaccgcg acaggacca aaaaccgtg 
      accgcctcg gtgggaacg caaaacagc tcgcaaatc 
      gtacaaacc gaaacccag tctcaaacc cagaccgaa 
      gcgacgaag actgagggg acgcaagcg ggaggcacg 
      ctccagatg aacacgaag acctcctcc acgtccacg 
      aggcctccg ctgagggac aacgcccac cacgtgctc 
      actaatacc aagtcggtg gactactcg gagatcgac 
      atgagggag atggacctg cgggcggcc caccaccac 
      aacctcctc aacaacaat atcagcaat aggaggagc 
      aagtcgaag agcacggag gatatgaac agggagaac 
      agcataagt ttggacagc aacacgttg aagaggatg 
      ctcaagccc atgcccagt gccgaaagc cccgtcaca 
      tcgccggaa atgggcaga cggaggtat aactattac 
      aataatcat caccacagg catccgaac aacaatggg 
      aggcattcg gaaccggag tcggggcat ccgcatccc 
      aggtccgcc atggcacaa aattcaagg ttctcgggc 
      tctagcttc aattacaga tcatcgcac gagatcggc 
      cggggatac cccggccgc ggcctgtac ctggaactt 
      gagcgcgga gctggcggt gacttatct ccaccttcc 
      gacaacgtt cttttcgac aaccaatgc tacgccaca 
      actccgagc tcaagtaac ggaaattcg gacccggaa 
      caaggcggg caccattat ggccgccag aggcacatg 
      caccaccag aacgccaat aacaccccg acccctggc 
      tcccccaca agccgcctt ctgctggaa tacgaaatg 
      cacctccgg aacactcta gccaaaggc atggacgcg 
      gagagctac agtctgcac acttttgaa gcccttctg 
      acccagagt atggaaaat ttagaattc gccgaaagc 
      cttcccgga tcgacccaa cgcagccct tacccttcc 
      cgaaaacga ccttcatcc agcgcttcc atcaacaaa 
      tcatcaact ttaccactg tcccatcgg ctgggcgcg 
      gtggcccgc gaccgcgaa cgcgacggc tactacagc 
      gaccgcaac gagctgctg cgcgaaagg gaaaaggaa 
      cgagaacgg ggatatttg agcgaccac aactccagt 
      ttcagttcg aggtgtgct tcgtgtata ggggagtcg 
      agtcgggcg cagtggttc cggcattct gatggctgg 
      aggtcgggc agctcgaac tttggctcg gggcagacg 
      ctgccgcaa gggcacaag aggtcgccc tgggattcg 
      ttaccttcg ctgaggcag gacagcagt ttgaatgat 
      agcggatat aagagcaat cgggccgac agctttgag 
      caacggggg atgttcgat cgccaggac agtcttcgc 
      tcggattac atgagtgat agagagtct tccagatat 
      ggaatcgtt cagcaggct tccatcgag agcgccgac 
      tcgaggctg tgctatctc acgtcgtca gagctggga 
      gtagttggg gcacatggg agcaacatg tggggggtg 
      agggatttt gtaacgagt ttcctcaag agtttggtt 
      ttaaatggg ggtgggcga attgtcaaa acagtgcct 
      tcattgttg gaaacacca agttatgtt tgcgcaaat 
      tttatcgga ttagaaacg atggtggag tcgaactcg 
      tgctcgagg aaatgcggc cgagtgtgt aacggggaa 
      acctcgccg gagaagtcg ctcatgtcc aagcgccgg 
      aggcacaag acggtgcac ttcggcgac aatctgctc 
      ctacaagtg tgcaccaac gccaatctg cagtcgcac 
      gtttcctac tcgaacatg gagcccaac gtccagcag 
      ctcttcagc ttcattgag acggtgttg agtgcgtgg 
      gtggccgaa gaagggctc acctcgcag ggcgaagtg 
      agcgaaatg gacgagaag gaactgcga aaacggaga 
      aacaagaaa cacggagcc aaagtcaaa agacttgac 
      atcaggcga ttggttggg gaagtgtcg aagttagat 
      ggacgattt ttgggaaat ctgagatac agacacatg 
      cactggaag ggaaacccc aactcggag ggctgtaac 
      gagctcttt ctcaggaag atatccgac gatgaccgc 
      atgtcgtta acgacggcg gtgtccgac gaagatgat 
      ggcgagagc gttatgaac tcgccttat aaagcgaaa 
      cagacggga accgccgct gcctctttc aactgcacc 
      ggagccgtc cggaaagcc gggtaagta ctccggagc 
      cgcttatta gcagtgtta cgtgaggtg gaaggcaat 
      aactactga tacaataaa tacacaaag tcaatgatg
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