Status
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Gene information
General information
TC014301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8729247..8733821
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014301
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08285

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014301 RA
      MQEGKIIAT FVALITLAS AQCPWQRES VDLQSSCLC 
      SYNLGHELS VQCDMVDWP KLLAALQKY ATATPLDLL 
      YVNNSTIGA LNDNIFVNL RVHNLQLSG CKINSVAGG 
      TFKGQEQHL KNLNLQDND LTEVPVESL RPLRTLSLL 
      DLSHNRISA VPDGAFETL KKLATLKLS DNNVTLSLE 
      ALKGLEGSL KNLNLKGTR QKDIPEAVK NLKTLAFLD 
      LSQNSLREL PGMTGLKSF EGLNSLTAL NLERNLIQK 
      LDKNAFQGV EKTLSSLSL LNNLLPDFP TEALSVLSE 
      LRVLDIGFN LLTELPDKA FQGNPSITL LALDGNPLA 
      TIPFSALSH LNNTLRGLS LGGRFLHCD CRLAWVIDW 
      IRNGDLQVT SRERNPQFC GSPPRFRDR GFYSIQPEE 
      LSCKKEEGV GIATVVTAD LDDVSIADS ELPPKTTTK 
      EPTTRKTTI TTSTISTTT TKTTSSTTK AATTKSKVR 
      TTSPPWKNT QRPPLIMNY PTKTKIDDA NDVIVKNAF 
      RQDNSVIIQ WGSETANIL GFRVVYRLF GDKSFKQGP 
      PLEASEREF KIKNVPSQE CIIVCVVSL EEINVTPET 
      VPYSQCREV RTVSTASSN MDKITIAAS AAICGTIVV 
      AVVIFVAAS RRRTRKLHE LHQQKIGMK NGLPISGLP 
      VNCCTGLGP AASPGGPLS SLATLSAFN NNKDWDQVS 
      GYSTHSVQR HNRMYSLDQ PSTLIADDM RSHFSTKGT 
      KSRSIADGQ SQNSFSNHS GRYLNAFPS NLITSRQDL 
      RQSRQSLTM QSDRLSTRG PSHHAPTHS VASSRRSRP 
      RSRSRDYNR PSSRYSTAG STHTLNNYC DNSDNWTDH 
      DMDIYMARN PTTRNGLVP L
    CDS sequence
    • >TC014301 RA
      atgcaagaa ggcaaaatc atcgccact tttgtggcc 
      ttaatcaca ctagcttca gctcaatgc ccttggcag 
      cgggaatcc gtagacctg cagtcgtca tgtctgtgt 
      tcctacaat ttaggacac gaactttcg gtgcaatgt 
      gacatggtc gactggcca aaacttctt gcagcttta 
      caaaagtac gccacagcg actcccctt gacttactc 
      tacgtgaac aattccacg attggtgct ttaaacgac 
      aatattttc gtgaacttg cgagtgcat aatttgcaa 
      ttatcgggg tgtaagata aacagtgtg gcgggtggt 
      accttcaaa ggacaggaa caacacttg aagaatttg 
      aatctgcaa gacaatgac ttgaccgaa gtgccggtt 
      gaaagtttg aggcctttg aggacttta tcacttctg 
      gatttgtcc cacaacagg attagtgcg gttccggac 
      ggtgcgttt gagacttta aaaaaatta gccacgttg 
      aagttatct gataacaac gtcacgtta tctttagag 
      gcgttaaag gggctcgaa ggctcccta aaaaatcta 
      aatctgaaa gggacgcgt cagaaagat atccccgaa 
      gcggtgaaa aatctaaaa actttggcg tttctggat 
      ttatcccaa aacagtttg cgggaactt ccgggaatg 
      acgggcttg aagtcattc gagggtttg aattcgctc 
      actgcgctt aatcttgag cgaaatttg atacaaaaa 
      ctggacaag aacgcgttc caaggtgtc gagaaaacg 
      ctaagttcg ctcagtttg ctcaataat ttgctccct 
      gattttccg acggaggct ttaagcgtc ttgtctgaa 
      ctgcgcgtg ttggatatc ggattcaat ttactgacc 
      gagttgcca gataaagcc tttcagggc aatccctcg 
      atcacattg ttggctctg gacggaaat ccgttagcc 
      accatccca ttttcggct ttgtctcac ttgaacaat 
      accctgcga ggtctcagc ttaggcggg cgatttctg 
      cactgcgat tgccgccta gcttgggtc atcgattgg 
      ataaggaac ggtgactta caagtgact agccgcgaa 
      cgaaacccc caattttgt ggcagtccg ccacggttc 
      agagaccgc ggcttctac agcatacag cccgaggaa 
      ctgtcctgt aaaaaagag gaaggcgtg ggcattgcc 
      acggtcgtc actgccgat ctggacgac gtttcaatc 
      gcggactct gaactacca ccgaaaact acaacaaag 
      gagccaacc accaggaaa acaaccatc acgaccagc 
      accatttcg acaacaacc acaaaaaca acctcctcg 
      accaccaaa gcagcaacg acgaaatcg aaagtcagg 
      acaacatcg ccaccgtgg aaaaacaca cagaggccg 
      cccctcatc atgaattac ccaaccaag acgaaaatt 
      gacgacgcg aacgatgtc atagtcaaa aacgccttc 
      cggcaagac aactcggtg ataatccaa tgggggtcc 
      gaaaccgcc aatatcttg ggcttcaga gttgtctat 
      cgcctattt ggagacaag agtttcaag cagggaccg 
      cccttagaa gcgagcgag agagaattc aagattaaa 
      aatgtgcca tcgcaggaa tgtatcata gtctgcgtg 
      gtctctctg gaagagatt aacgtcaca cccgaaacc 
      gttccatat tcacaatgt cgtgaagtg agaacagtt 
      tccaccgct tcttccaac atggacaaa atcacaatt 
      gctgcaagt gcagcgatt tgtggaacc attgtagtt 
      gccgtggtc attttcgta gcggcttcg agaagaaga 
      acgcgaaaa ttgcacgaa ttacaccaa cagaaaatc 
      gggatgaaa aacggactg cccataagt gggttaccg 
      gtcaactgt tgcaccggt ttagggcct gcagccagt 
      cccggcgga cctctgtcc tcgctggcg acgctcagt 
      gcgttcaac aacaacaaa gactgggac caagtttcg 
      ggctacagc acgcacagc gtccagaga cacaaccgg 
      atgtactcg ctggaccag ccctccacc ctaatcgcg 
      gacgacatg cgttcccac ttcagtaca aaaggcacg 
      aaatcgcgt tcgatagcc gacggacaa tcccagaac 
      agtttttcc aaccactct ggacgctac ttgaacgca 
      tttcccagt aacttaata acttcgcgt caagatttg 
      cgccaatcg agacagtcc ttgaccatg caatcggac 
      agactgtcg actcgtgga cctagtcac cacgcaccg 
      acccattcc gtggcgtcc tccagacgc tccagaccc 
      agatcccgg tcccgggac tacaacagg cccagtagc 
      cggtacagc acggctgga tcgactcac actttaaac 
      aattattgc gacaattcc gacaactgg actgaccac 
      gatatggac atttacatg gcgagaaac ccgaccact 
      aggaacggt ctcgtaccg ctctga
    mRNA sequence
    • >TC014301 RA
      tttggcttc aaaagagag aatgacagt gtgtctttt 
      gcaggttgg ttgaccata acggtccta taatcttca 
      cctagagcc cgaggccgc ggataccca ccaaccaag 
      gcccatcct taagacctt tggtatttt gcagccgaa 
      acaatgatc gatcagcat gttacataa cggcagaat 
      ggacatcaa atgttgcta tcataggtt gtggcaaaa 
      catttagat catgcaaga aggcaaaat catcgccac 
      ttttgtggc cttaatcac actagcttc agctcaatg 
      cccttggca gcgggaatc cgtagacct gcagtcgtc 
      atgtctgtg ttcctacaa tttaggaca cgaactttc 
      ggtgcaatg tgacatggt cgactggcc aaaacttct 
      tgcagcttt acaaaagta cgccacagc gactcccct 
      tgacttact ctacgtgaa caattccac gattggtgc 
      tttaaacga caatatttt cgtgaactt gcgagtgca 
      taatttgca attatcggg gtgtaagat aaacagtgt 
      ggcgggtgg taccttcaa aggacagga acaacactt 
      gaagaattt gaatctgca agacaatga cttgaccga 
      agtgccggt tgaaagttt gaggccttt gaggacttt 
      atcacttct ggatttgtc ccacaacag gattagtgc 
      ggttccgga cggtgcgtt tgagacttt aaaaaaatt 
      agccacgtt gaagttatc tgataacaa cgtcacgtt 
      atctttaga ggcgttaaa ggggctcga aggctccct 
      aaaaaatct aaatctgaa agggacgcg tcagaaaga 
      tatccccga agcggtgaa aaatctaaa aactttggc 
      gtttctgga tttatccca aaacagttt gcgggaact 
      tccgggaat gacgggctt gaagtcatt cgagggttt 
      gaattcgct cactgcgct taatcttga gcgaaattt 
      gatacaaaa actggacaa gaacgcgtt ccaaggtgt 
      cgagaaaac gctaagttc gctcagttt gctcaataa 
      tttgctccc tgattttcc gacggaggc tttaagcgt 
      cttgtctga actgcgcgt gttggatat cggattcaa 
      tttactgac cgagttgcc agataaagc ctttcaggg 
      caatccctc gatcacatt gttggctct ggacggaaa 
      tccgttagc caccatccc attttcggc tttgtctca 
      cttgaacaa taccctgcg aggtctcag cttaggcgg 
      gcgatttct gcactgcga ttgccgcct agcttgggt 
      catcgattg gataaggaa cggtgactt acaagtgac 
      tagccgcga acgaaaccc ccaattttg tggcagtcc 
      gccacggtt cagagaccg cggcttcta cagcataca 
      gcccgagga actgtcctg taaaaaaga ggaaggcgt 
      gggcattgc cacggtcgt cactgccga tctggacga 
      cgtttcaat cgcggactc tgaactacc accgaaaac 
      tacaacaaa ggagccaac caccaggaa aacaaccat 
      cacgaccag caccatttc gacaacaac cacaaaaac 
      aacctcctc gaccaccaa agcagcaac gacgaaatc 
      gaaagtcag gacaacatc gccaccgtg gaaaaacac 
      acagaggcc gcccctcat catgaatta cccaaccaa 
      gacgaaaat tgacgacgc gaacgatgt catagtcaa 
      aaacgcctt ccggcaaga caactcggt gataatcca 
      atgggggtc cgaaaccgc caatatctt gggcttcag 
      agttgtcta tcgcctatt tggagacaa gagtttcaa 
      gcagggacc gcccttaga agcgagcga gagagaatt 
      caagattaa aaatgtgcc atcgcagga atgtatcat 
      agtctgcgt ggtctctct ggaagagat taacgtcac 
      acccgaaac cgttccata ttcacaatg tcgtgaagt 
      gagaacagt ttccaccgc ttcttccaa catggacaa 
      aatcacaat tgctgcaag tgcagcgat ttgtggaac 
      cattgtagt tgccgtggt cattttcgt agcggcttc 
      gagaagaag aacgcgaaa attgcacga attacacca 
      acagaaaat cgggatgaa aaacggact gcccataag 
      tgggttacc ggtcaactg ttgcaccgg tttagggcc 
      tgcagccag tcccggcgg acctctgtc ctcgctggc 
      gacgctcag tgcgttcaa caacaacaa agactggga 
      ccaagtttc gggctacag cacgcacag cgtccagag 
      acacaaccg gatgtactc gctggacca gccctccac 
      cctaatcgc ggacgacat gcgttccca cttcagtac 
      aaaaggcac gaaatcgcg ttcgatagc cgacggaca 
      atcccagaa cagtttttc caaccactc tggacgcta 
      cttgaacgc atttcccag taacttaat aacttcgcg 
      tcaagattt gcgccaatc gagacagtc cttgaccat 
      gcaatcgga cagactgtc gactcgtgg acctagtca 
      ccacgcacc gacccattc cgtggcgtc ctccagacg 
      ctccagacc cagatcccg gtcccggga ctacaacag 
      gcccagtag ccggtacag cacggctgg atcgactca 
      cactttaaa caattattg cgacaattc cgacaactg 
      gactgacca cgatatgga catttacat ggcgagaaa 
      cccgaccac taggaacgg tctcgtacc gctctgatt 
      tagttgctc aatgtgttc gcaaggaat gattcaaaa 
      aggctgtta tttgattca gacaattcg caacagcac 
      gactgatag taggatcag ttaacaagt aattttaaa 
      gatagttac tagagaata atgataatg tatttttat 
      atttaaacg atatttctc tattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY