| General information | |||
| TC014301 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 8729247..8733821 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014301 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08285 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014301 RA
MQEGKIIAT FVALITLAS AQCPWQRES VDLQSSCLC SYNLGHELS VQCDMVDWP KLLAALQKY ATATPLDLL YVNNSTIGA LNDNIFVNL RVHNLQLSG CKINSVAGG TFKGQEQHL KNLNLQDND LTEVPVESL RPLRTLSLL DLSHNRISA VPDGAFETL KKLATLKLS DNNVTLSLE ALKGLEGSL KNLNLKGTR QKDIPEAVK NLKTLAFLD LSQNSLREL PGMTGLKSF EGLNSLTAL NLERNLIQK LDKNAFQGV EKTLSSLSL LNNLLPDFP TEALSVLSE LRVLDIGFN LLTELPDKA FQGNPSITL LALDGNPLA TIPFSALSH LNNTLRGLS LGGRFLHCD CRLAWVIDW IRNGDLQVT SRERNPQFC GSPPRFRDR GFYSIQPEE LSCKKEEGV GIATVVTAD LDDVSIADS ELPPKTTTK EPTTRKTTI TTSTISTTT TKTTSSTTK AATTKSKVR TTSPPWKNT QRPPLIMNY PTKTKIDDA NDVIVKNAF RQDNSVIIQ WGSETANIL GFRVVYRLF GDKSFKQGP PLEASEREF KIKNVPSQE CIIVCVVSL EEINVTPET VPYSQCREV RTVSTASSN MDKITIAAS AAICGTIVV AVVIFVAAS RRRTRKLHE LHQQKIGMK NGLPISGLP VNCCTGLGP AASPGGPLS SLATLSAFN NNKDWDQVS GYSTHSVQR HNRMYSLDQ PSTLIADDM RSHFSTKGT KSRSIADGQ SQNSFSNHS GRYLNAFPS NLITSRQDL RQSRQSLTM QSDRLSTRG PSHHAPTHS VASSRRSRP RSRSRDYNR PSSRYSTAG STHTLNNYC DNSDNWTDH DMDIYMARN PTTRNGLVP L
- >TC014301 RA
atgcaagaa ggcaaaatc atcgccact tttgtggcc ttaatcaca ctagcttca gctcaatgc ccttggcag cgggaatcc gtagacctg cagtcgtca tgtctgtgt tcctacaat ttaggacac gaactttcg gtgcaatgt gacatggtc gactggcca aaacttctt gcagcttta caaaagtac gccacagcg actcccctt gacttactc tacgtgaac aattccacg attggtgct ttaaacgac aatattttc gtgaacttg cgagtgcat aatttgcaa ttatcgggg tgtaagata aacagtgtg gcgggtggt accttcaaa ggacaggaa caacacttg aagaatttg aatctgcaa gacaatgac ttgaccgaa gtgccggtt gaaagtttg aggcctttg aggacttta tcacttctg gatttgtcc cacaacagg attagtgcg gttccggac ggtgcgttt gagacttta aaaaaatta gccacgttg aagttatct gataacaac gtcacgtta tctttagag gcgttaaag gggctcgaa ggctcccta aaaaatcta aatctgaaa gggacgcgt cagaaagat atccccgaa gcggtgaaa aatctaaaa actttggcg tttctggat ttatcccaa aacagtttg cgggaactt ccgggaatg acgggcttg aagtcattc gagggtttg aattcgctc actgcgctt aatcttgag cgaaatttg atacaaaaa ctggacaag aacgcgttc caaggtgtc gagaaaacg ctaagttcg ctcagtttg ctcaataat ttgctccct gattttccg acggaggct ttaagcgtc ttgtctgaa ctgcgcgtg ttggatatc ggattcaat ttactgacc gagttgcca gataaagcc tttcagggc aatccctcg atcacattg ttggctctg gacggaaat ccgttagcc accatccca ttttcggct ttgtctcac ttgaacaat accctgcga ggtctcagc ttaggcggg cgatttctg cactgcgat tgccgccta gcttgggtc atcgattgg ataaggaac ggtgactta caagtgact agccgcgaa cgaaacccc caattttgt ggcagtccg ccacggttc agagaccgc ggcttctac agcatacag cccgaggaa ctgtcctgt aaaaaagag gaaggcgtg ggcattgcc acggtcgtc actgccgat ctggacgac gtttcaatc gcggactct gaactacca ccgaaaact acaacaaag gagccaacc accaggaaa acaaccatc acgaccagc accatttcg acaacaacc acaaaaaca acctcctcg accaccaaa gcagcaacg acgaaatcg aaagtcagg acaacatcg ccaccgtgg aaaaacaca cagaggccg cccctcatc atgaattac ccaaccaag acgaaaatt gacgacgcg aacgatgtc atagtcaaa aacgccttc cggcaagac aactcggtg ataatccaa tgggggtcc gaaaccgcc aatatcttg ggcttcaga gttgtctat cgcctattt ggagacaag agtttcaag cagggaccg cccttagaa gcgagcgag agagaattc aagattaaa aatgtgcca tcgcaggaa tgtatcata gtctgcgtg gtctctctg gaagagatt aacgtcaca cccgaaacc gttccatat tcacaatgt cgtgaagtg agaacagtt tccaccgct tcttccaac atggacaaa atcacaatt gctgcaagt gcagcgatt tgtggaacc attgtagtt gccgtggtc attttcgta gcggcttcg agaagaaga acgcgaaaa ttgcacgaa ttacaccaa cagaaaatc gggatgaaa aacggactg cccataagt gggttaccg gtcaactgt tgcaccggt ttagggcct gcagccagt cccggcgga cctctgtcc tcgctggcg acgctcagt gcgttcaac aacaacaaa gactgggac caagtttcg ggctacagc acgcacagc gtccagaga cacaaccgg atgtactcg ctggaccag ccctccacc ctaatcgcg gacgacatg cgttcccac ttcagtaca aaaggcacg aaatcgcgt tcgatagcc gacggacaa tcccagaac agtttttcc aaccactct ggacgctac ttgaacgca tttcccagt aacttaata acttcgcgt caagatttg cgccaatcg agacagtcc ttgaccatg caatcggac agactgtcg actcgtgga cctagtcac cacgcaccg acccattcc gtggcgtcc tccagacgc tccagaccc agatcccgg tcccgggac tacaacagg cccagtagc cggtacagc acggctgga tcgactcac actttaaac aattattgc gacaattcc gacaactgg actgaccac gatatggac atttacatg gcgagaaac ccgaccact aggaacggt ctcgtaccg ctctga
- >TC014301 RA
tttggcttc aaaagagag aatgacagt gtgtctttt gcaggttgg ttgaccata acggtccta taatcttca cctagagcc cgaggccgc ggataccca ccaaccaag gcccatcct taagacctt tggtatttt gcagccgaa acaatgatc gatcagcat gttacataa cggcagaat ggacatcaa atgttgcta tcataggtt gtggcaaaa catttagat catgcaaga aggcaaaat catcgccac ttttgtggc cttaatcac actagcttc agctcaatg cccttggca gcgggaatc cgtagacct gcagtcgtc atgtctgtg ttcctacaa tttaggaca cgaactttc ggtgcaatg tgacatggt cgactggcc aaaacttct tgcagcttt acaaaagta cgccacagc gactcccct tgacttact ctacgtgaa caattccac gattggtgc tttaaacga caatatttt cgtgaactt gcgagtgca taatttgca attatcggg gtgtaagat aaacagtgt ggcgggtgg taccttcaa aggacagga acaacactt gaagaattt gaatctgca agacaatga cttgaccga agtgccggt tgaaagttt gaggccttt gaggacttt atcacttct ggatttgtc ccacaacag gattagtgc ggttccgga cggtgcgtt tgagacttt aaaaaaatt agccacgtt gaagttatc tgataacaa cgtcacgtt atctttaga ggcgttaaa ggggctcga aggctccct aaaaaatct aaatctgaa agggacgcg tcagaaaga tatccccga agcggtgaa aaatctaaa aactttggc gtttctgga tttatccca aaacagttt gcgggaact tccgggaat gacgggctt gaagtcatt cgagggttt gaattcgct cactgcgct taatcttga gcgaaattt gatacaaaa actggacaa gaacgcgtt ccaaggtgt cgagaaaac gctaagttc gctcagttt gctcaataa tttgctccc tgattttcc gacggaggc tttaagcgt cttgtctga actgcgcgt gttggatat cggattcaa tttactgac cgagttgcc agataaagc ctttcaggg caatccctc gatcacatt gttggctct ggacggaaa tccgttagc caccatccc attttcggc tttgtctca cttgaacaa taccctgcg aggtctcag cttaggcgg gcgatttct gcactgcga ttgccgcct agcttgggt catcgattg gataaggaa cggtgactt acaagtgac tagccgcga acgaaaccc ccaattttg tggcagtcc gccacggtt cagagaccg cggcttcta cagcataca gcccgagga actgtcctg taaaaaaga ggaaggcgt gggcattgc cacggtcgt cactgccga tctggacga cgtttcaat cgcggactc tgaactacc accgaaaac tacaacaaa ggagccaac caccaggaa aacaaccat cacgaccag caccatttc gacaacaac cacaaaaac aacctcctc gaccaccaa agcagcaac gacgaaatc gaaagtcag gacaacatc gccaccgtg gaaaaacac acagaggcc gcccctcat catgaatta cccaaccaa gacgaaaat tgacgacgc gaacgatgt catagtcaa aaacgcctt ccggcaaga caactcggt gataatcca atgggggtc cgaaaccgc caatatctt gggcttcag agttgtcta tcgcctatt tggagacaa gagtttcaa gcagggacc gcccttaga agcgagcga gagagaatt caagattaa aaatgtgcc atcgcagga atgtatcat agtctgcgt ggtctctct ggaagagat taacgtcac acccgaaac cgttccata ttcacaatg tcgtgaagt gagaacagt ttccaccgc ttcttccaa catggacaa aatcacaat tgctgcaag tgcagcgat ttgtggaac cattgtagt tgccgtggt cattttcgt agcggcttc gagaagaag aacgcgaaa attgcacga attacacca acagaaaat cgggatgaa aaacggact gcccataag tgggttacc ggtcaactg ttgcaccgg tttagggcc tgcagccag tcccggcgg acctctgtc ctcgctggc gacgctcag tgcgttcaa caacaacaa agactggga ccaagtttc gggctacag cacgcacag cgtccagag acacaaccg gatgtactc gctggacca gccctccac cctaatcgc ggacgacat gcgttccca cttcagtac aaaaggcac gaaatcgcg ttcgatagc cgacggaca atcccagaa cagtttttc caaccactc tggacgcta cttgaacgc atttcccag taacttaat aacttcgcg tcaagattt gcgccaatc gagacagtc cttgaccat gcaatcgga cagactgtc gactcgtgg acctagtca ccacgcacc gacccattc cgtggcgtc ctccagacg ctccagacc cagatcccg gtcccggga ctacaacag gcccagtag ccggtacag cacggctgg atcgactca cactttaaa caattattg cgacaattc cgacaactg gactgacca cgatatgga catttacat ggcgagaaa cccgaccac taggaacgg tctcgtacc gctctgatt tagttgctc aatgtgttc gcaaggaat gattcaaaa aggctgtta tttgattca gacaattcg caacagcac gactgatag taggatcag ttaacaagt aattttaaa gatagttac tagagaata atgataatg tatttttat atttaaacg atatttctc tattt
