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Gene information
General information
TC013505 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8692798..8697285
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013505
    iBeetle screen iB_08284

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013505 RA
      MHFRCFFSQ IDNPMHQTN NRVTLNIVG KSSDPSLAK 
QWSSPAKPK YGLLIRSRA PSRSHVKAL IHETFLAKL 
LISRFYFCE FACCEMFVI CIAAAVLST VTADSSGIK 
NLAGEPDYR QVRLHWEVD DDSQSEFQV RYCELQTWG 
AQRCRTQMV NESEENEVD DHAKGYSVE VKGLRMATT 
YSFEVKEQG ERQDRAEGD DKDENIIVI PTKGFSAKA 
TQCLPHASE IEVSTGPYF GGRIAVEAA DGERCALDG 
EASSPRDTY TLRINHTEC GSQVNETTV ATFVLVQEN 
LPILTHSTR RFLVLCSYQ PETLTVRAG INLPTGHSG 
RGQAHMAPV PYEEDNTLG RRGRNFRTG RLLQKPEAL 
VKEETEASE SVSRTNAVP VIVIAMLSI AGVIGIIAI 
AIKITNKKL LEDLDNISI ASEVTTSSC STSPDSEIS 
CHSQPRSVI HV
    CDS sequence
    • >TC013505 RA
      atgcatttt cgatgtttc ttttcgcaa atcgacaat 
ccaatgcac caaaccaac aacagagtg acgttaaat 
attgtgggg aagagcagt gacccctcc ttggcgaaa 
cagtggtcg tctccagcg aaaccgaag tacggactg 
ttaatccgc tcgcgagca ccaagccgg tcccacgta 
aaagcgctc atacatgaa acatttctc gcaaaactg 
ctcatttct cggttttat ttttgtgaa tttgcgtgc 
tgtgaaatg tttgttatt tgcatcgcc gctgctgtt 
ttgagcaca gtgactgcg gattcttcc ggcatcaaa 
aacctcgcc ggcgaaccg gattataga caagtgcgg 
ctacattgg gaagtcgac gatgattca caaagcgaa 
tttcaagtc cggtattgt gaactccag acgtggggt 
gcacagaga tgtcggacc caaatggtg aatgagtcc 
gaggagaat gaggtggac gatcacgcg aagggctat 
agcgtcgaa gtgaaaggg ctcaggatg gccaccacg 
tactcgttt gaggtcaaa gagcagggc gaacggcag 
gacagggca gagggcgac gacaaggac gaaaacatc 
attgtgata cccaccaaa ggattttca gcgaaggcg 
acgcagtgc ctgccccac gccagcgag atcgaagta 
tcaacaggt ccgtacttc ggggggcgc atagccgtg 
gaggcggcg gacggggag cggtgcgcc ctggacggg 
gaggcgagc agcccccgg gacacctac accctccgc 
atcaaccac acggagtgc ggcagccaa gtgaacgag 
accacggtc gcgactttc gttctcgtg caagagaac 
ctcccgatt ttgacgcac agcacgcgg aggttcctc 
gtactgtgc tcgtaccaa ccggaaacg ctcaccgta 
cgtgccgga ataaatcta ccaacggga cattccggt 
cgtggccaa gcacacatg gctccggtg ccgtacgaa 
gaagacaac acgctcggc agaagaggc cgcaatttc 
cgaactggg agattactg caaaaacct gaagcgcta 
gtaaaagag gaaaccgaa gcttcggaa agtgtgtcc 
agaacgaac gccgtgccc gtgattgtg atagcgatg 
ctgtcaatc gctggagtt atcggaatc atcgctata 
gctataaaa atcaccaac aagaagctg cttgaagac 
ctagacaac atctctatt gcttccgag gtgactaca 
agcagctgc agcacatca ccagacagc gaaatatct 
tgccacagt cagccccgg tccgtgata catgtataa
    mRNA sequence
    • >TC013505 RA
      tgaaatgca attttccag acaagactc acacagcgg 
cggccacct ctggtgctt acccgttga aaataatgc 
attttcgat gtttctttt cgcaaatcg acaatccaa 
tgcaccaaa ccaacaaca gagtgacgt taaatattg 
tggggaaga gcagtgacc cctccttgg cgaaacagt 
ggtcgtctc cagcgaaac cgaagtacg gactgttaa 
tccgctcgc gagcaccaa gccggtccc acgtaaaag 
cgctcatac atgaaacat ttctcgcaa aactgctca 
tttctcggt tttattttt gtgaatttg cgtgctgtg 
aaatgtttg ttatttgca tcgccgctg ctgttttga 
gcacagtga ctgcggatt cttccggca tcaaaaacc 
tcgccggcg aaccggatt atagacaag tgcggctac 
attgggaag tcgacgatg attcacaaa gcgaatttc 
aagtccggt attgtgaac tccagacgt ggggtgcac 
agagatgtc ggacccaaa tggtgaatg agtccgagg 
agaatgagg tggacgatc acgcgaagg gctatagcg 
tcgaagtga aagggctca ggatggcca ccacgtact 
cgtttgagg tcaaagagc agggcgaac ggcaggaca 
gggcagagg gcgacgaca aggacgaaa acatcattg 
tgataccca ccaaaggat tttcagcga aggcgacgc 
agtgcctgc cccacgcca gcgagatcg aagtatcaa 
caggtccgt acttcgggg ggcgcatag ccgtggagg 
cggcggacg gggagcggt gcgccctgg acggggagg 
cgagcagcc cccgggaca cctacaccc tccgcatca 
accacacgg agtgcggca gccaagtga acgagacca 
cggtcgcga ctttcgttc tcgtgcaag agaacctcc 
cgattttga cgcacagca cgcggaggt tcctcgtac 
tgtgctcgt accaaccgg aaacgctca ccgtacgtg 
ccggaataa atctaccaa cgggacatt ccggtcgtg 
gccaagcac acatggctc cggtgccgt acgaagaag 
acaacacgc tcggcagaa gaggccgca atttccgaa 
ctgggagat tactgcaaa aacctgaag cgctagtaa 
aagaggaaa ccgaagctt cggaaagtg tgtccagaa 
cgaacgccg tgcccgtga ttgtgatag cgatgctgt 
caatcgctg gagttatcg gaatcatcg ctatagcta 
taaaaatca ccaacaaga agctgcttg aagacctag 
acaacatct ctattgctt ccgaggtga ctacaagca 
gctgcagca catcaccag acagcgaaa tatcttgcc 
acagtcagc cccggtccg tgatacatg tataacgtg 
atcaccgat ttaatgaaa cctagtacc aagtgtgat 
tacaattta ataaactaa tttataatt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY