Status
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Gene information
General information
TC013515 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8534026..8538713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013515
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08282

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013515 RA
      MTQILGNEK QGLVFEILS ARICDEGEE RKHVVYTLQ 
      VRHISGNDD LSPSVVERR YTHFLNLYS ALKKEHPNL 
      MTNVTFPKK VIIGNFDNE LISTRSTGF ESLLRHICT 
      ESKLRASKA LVEFLQGVE VEKARTFLE KRDFSSALP 
      LLENNFKLL NKFYTDRSP VVLIALCRL LGCCMSIPE 
      CSQCQKWAD LALHRYEGV SDSDLLELY VPLLNACIT 
      IWWQNGRNK DLLEQRLQD LKRQGVKIN PQSTLLDEV 
      KAVEGKIFG L
    CDS sequence
    • >TC013515 RA
      atgacccaa atcttaggc aacgaaaag cagggacta 
      gttttcgaa atactgtca gcgcgaata tgtgacgaa 
      ggcgaggaa aggaaacat gtagtgtac acgctgcaa 
      gtgcgtcac atttcagga aacgacgat ttgagccct 
      tcggtggtg gagcggcga tacacgcac ttcctgaac 
      ctttacagc gccctaaag aaggaacac ccgaactta 
      atgaccaat gttacattt cccaaaaaa gtgataata 
      ggaaacttt gataatgag ctgatttcc accagaagc 
      acgggcttt gagtccctg ttgaggcac atttgcacc 
      gaatctaaa ctgcgagcg tctaaggct ttagtcgag 
      ttccttcaa ggcgttgaa gtggagaaa gcacggacg 
      tttttggaa aaacgggac ttttcgtcc gctttgccg 
      ctgcttgaa aacaatttt aaactacta aacaaattt 
      tatactgat cggtcgccg gtcgtgctt atcgcttta 
      tgtagactg ttagggtgc tgcatgtca attcccgag 
      tgttcacaa tgccaaaaa tgggctgat ttagcccta 
      catagatat gagggggtt agtgatagt gatttgttg 
      gagttgtat gtaccttta ttgaatgct tgtatcaca 
      atttggtgg cagaatgga cgaaataaa gatctgctt 
      gagcagcgg ttgcaagat ttgaagaga caaggggtc 
      aaaatcaat ccgcaatcg actttgttg gacgaagtg 
      aaggcagtg gagggaaag atatttggg ttgtag
    mRNA sequence
    • >TC013515 RA
      cgccactgc tgcgggtac agaaataaa gcagcattt 
      tacttttgc taaaaattt ctttatttg ttttttttt 
      attacgttc tttgaaatg ctttgagta agtgaaggt 
      aaaggtaaa agaaacaca gtgttcaac aaaaatctt 
      tattgtata aataaatag cagttttaa aaaaatact 
      ttgcttaac gaaacaggt gatcttatg agttgtagt 
      tacatgaaa catcacaca aattgaaca acacttacg 
      atacaatac tttttaaat tctcataag ccgcattca 
      tcactcccc agctgataa atgctctcg tacagttgg 
      tctgaactc cgtgataaa tatacctaa ttttcctac 
      ctctttcgt tataaattt gtactaaaa ccttaaaaa 
      tattctcat agccgcccc caatcttgc tctgcatcg 
      caactttga gaacattaa gtggataaa acacgctgg 
      acaaaaaca cacctatca tagcaccac aaaaaaatt 
      cgctaagag tttcgtaaa actttgaat taaacctag 
      gtctgtagt attgtactt catccctga tgacaaaaa 
      agcccacat tggagggcc catttcgga gcaaaaccg 
      ggaccaaac cccggtagt actcccgaa acccgtggt 
      tgagtccta caactttaa atgcatgca gcgtgttcc 
      tgaactcct tgtggtaat gtgcgtccg caagcagcg 
      tctgtatcc gctcaaacg gcatcaaga tcgcctcgg 
      tggttcccg caaccatgc ccccggcaa acttcgaca 
      aatacccat taaagcccg acagcataa gcgggcgcc 
      gcacctcct cgtaaatcc caaacatta gcgacatcg 
      agagcgtct tttggcaca aagggggca gcatgcccc 
      gatataaat aaaacattc cttctttct gcagctggc 
      gaaacgcac ttaacgcgt taacgccat gcaacatct 
      gaaactagc cgtttaagc cccaatttg tgggaaaat 
      tagcgcaat aacctgtcg aaaaatcac tttgtttat 
      cggataggt tattgtaat gttaataaa tgcagcgct 
      ccaaccgca tgcaaactc cttccaatt tatctggta 
      aatgtcctc cagaaccag ggtgtcccc aaccgttgg 
      gtcataatc ctcgatttt atcaatgtc cagcatact 
      gacattcga gacaaattt tgacatctg tcaacatct 
      taggttatg taactccga aaggcgaac tcaccccct 
      tacctttaa taacaattt ggtttactt gactaggga 
      tacaattat cacaaattc ataatttac ttcagttca 
      ggggtgtta acaaatcat ttttgtgcc agtaattcg 
      cgcatcgca gcgggcgtg acgtcacta ctgacgttt 
      aggttaatt attgacaag tgacagtga cgtttttta 
      tctaccttt tgttgttat ctgcaaaat gtgtacaca 
      aaggtgtat aaaagtact tgtaggtgg ggataaggg 
      caatattga ctgcgtggg ggcgtaatt atgtgaaca 
      aaacatctc aaatatgac ccaaatctt aggcaacga 
      aaagcaggg actagtttt cgaaatact gtcagcgcg 
      aatatgtga cgaaggcga ggaaaggaa acatgtagt 
      gtacacgct gcaagtgcg tcacatttc aggaaacga 
      cgatttgag cccttcggt ggtggagcg gcgatacac 
      gcacttcct gaaccttta cagcgccct aaagaagga 
      acacccgaa cttaatgac caatgttac atttcccaa 
      aaaagtgat aataggaaa ctttgataa tgagctgat 
      ttccaccag aagcacggg ctttgagtc cctgttgag 
      gcacatttg caccgaatc taaactgcg agcgtctaa 
      ggctttagt cgagttcct tcaaggcgt tgaagtgga 
      gaaagcacg gacgttttt ggaaaaacg ggacttttc 
      gtccgcttt gccgctgct tgaaaacaa ttttaaact 
      actaaacaa attttatac tgatcggtc gccggtcgt 
      gcttatcgc tttatgtag actgttagg gtgctgcat 
      gtcaattcc cgagtgttc acaatgcca aaaatgggc 
      tgatttagc cctacatag atatgaggg ggttagtga 
      tagtgattt gttggagtt gtatgtacc tttattgaa 
      tgcttgtat cacaatttg gtggcagaa tggacgaaa 
      taaagatct gcttgagca gcggttgca agatttgaa 
      gagacaagg ggtcaaaat caatccgca atcgacttt 
      gttggacga agtgaaggc agtggaggg aaagatatt 
      tgggttgta gcaattttc acaccagtg ttgtgtact 
      taattagca tcgcgatat tttactgat attgttaac 
      gtatttatt ttggagttt tatttgttt gacgactga 
      tatttgcag ttatgggta gcaatactc aatattttc 
      tgttttatt cacgcctga atatacatc ttgtttatt 
      tgctttttg ttttagtac tttgagcaa aaaatagat 
      aataatttt ttttttgta ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY