Status
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Gene information
General information
TC033117
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013527,TC013526
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8410760..8429943
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033117
    iBeetle screen iB_08281

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033117 RA
      MAVFGSFCL IFLVLRSVA TIEDYCSLN TYSTNGTYM 
      QFTKEPNVD EFAIAGKFK GLHCCAKGY RSIEWYKDG 
      KPYPWPLMV SQLILYPES ANQTVYTQS VTEADAGNY 
      TCLLRNDSV VHSHTIQLK VFEKIPDDP KITYVSKDH 
      QVPLGQQLR LFCEAFAGR VDLPDAHNE VVWRKSGHN 
      GTLDDEPRV RQEKISRED GQTFGTYLI IDSVRREDF 
      GEYVCKISK PGKSIEQRV FVDEKIEVE YLNPNPVPV 
      AKLLLCLSA IIFLCLAVV ILNLQYGLP LRVRFKDTF 
      GVVEESDGK TNDVLLLYS SKDSELALG TLLPILESR 
      YNYKCVHRE LPSSINYWF GELQNDALK SRRIVTLLS 
      PPVVSDNWT SAAIYQAIK QLQALGSKL TIITLKELP 
      RCENETKNS HGETLTILL RTINVVHWE RTKEEKFWL 
      NLRLRLPPK RRNERLVDG RSQTQGINS SRLSNDSEE 
      SSDLV
    CDS sequence
    • >TC033117 RA
      atggcagtg ttcggcagt ttctgtttg attttcttg 
      gttttgcgt tcggtggca accatcgag gattattgc 
      tctttgaac acctacagc accaatggg acttacatg 
      cagtttacg aaagaaccc aacgtggac gagttcgcg 
      atcgcaggg aagtttaaa ggactacat tgctgcgcg 
      aaaggatac cgcagcatc gaatggtat aaagacggc 
      aaaccgtat ccgtggcct ttaatggta tcacagtta 
      atcctctac cctgagagt gccaatcaa acggtatac 
      acacagtcc gttacggaa gccgacgct ggaaattac 
      acttgttta ttgaggaac gactccgta gttcactct 
      cacacaatc cagctcaaa gtcttcgaa aaaattcca 
      gacgatccg aaaatcacg tacgtgtca aaggaccac 
      caggtgccg ttgggccaa cagctccgc ctcttctgc 
      gaggccttt gctggacga gtggatcta ccagatgca 
      cacaatgag gttgtgtgg cgcaagtcg ggacataat 
      ggaacgctt gatgacgag cctcgagtt agacaggaa 
      aaaatctcc agagaggac gggcaaact ttcggtact 
      tacctgata atcgacagc gtccggaga gaagacttc 
      ggcgagtac gtctgtaaa ataagcaaa cccggaaaa 
      agcatcgag cagcgggtc tttgtggac gaaaaaatc 
      gaagtggaa tatttaaac cctaatccc gtaccagtg 
      gcaaagctt ctcctttgc ctgtctgcg attattttt 
      ctgtgcttg gcggttgtg atcctcaat cttcagtac 
      ggtttgccg ttgcgggtg cgctttaag gacaccttc 
      ggcgtcgtt gaagaatca gacgggaaa acgaacgac 
      gttctcctt ctgtattca tcaaaggac agcgaactg 
      gctttgggg acccttttg ccaatcttg gaatcaaga 
      tacaattac aaatgtgtc cacagggaa ctcccctcg 
      agtatcaac tactggttc ggggaattg caaaatgac 
      gctctaaag agccgcaga attgtaacg cttctctct 
      ccgccagtg gttagtgac aactggact tctgcggct 
      atttaccaa gctataaaa cagctacaa gcgttaggg 
      tccaagctg acgattatc acgctgaag gagcttcca 
      cgatgcgag aatgaaacg aaaaactcg cacggggag 
      acgctgacc attttgttg aggacgatc aatgtagtg 
      cattgggag aggacgaag gaggagaaa ttttggctt 
      aacttgagg ctgcggctg cctccaaaa aggcgaaat 
      gaacgactg gtagacggt agaagtcaa acgcagggt 
      ataaattct tcaagactg agcaacgac tctgaagag 
      agttccgat cttgtgtaa
    mRNA sequence
    • >TC033117 RA
      gtgaacccc ctgtgtcat ttttctccc actcccata 
      tgatttaaa cgaaactcc ccgagctca aacaggtga 
      attccgaat agtaccgaa caccaattc gtatttccc 
      cctatttct catttcacg tgtgattgt tgtggcgaa 
      gagttgtgt gcggtgact tgagtgact acgtaaacc 
      gccagttct ccatgtgac ttgaagatg actcactaa 
      gtgttctct cgcgtattt tccaccttt tcttaaata 
      tgctagatt ttaattctt cacctcatc gaaaaaacc 
      gacttaatt tagccacag tgactcatt agaagataa 
      ttagaattg catcagtcg ctataaaaa tggcagtgt 
      tcggcagtt tctgtttga ttttcttgg ttttgcgtt 
      cggtggcaa ccatcgagg attattgct ctttgaaca 
      cctacagca ccaatggga cttacatgc agtttacga 
      aagaaccca acgtggacg agttcgcga tcgcaggga 
      agtttaaag gactacatt gctgcgcga aaggatacc 
      gcagcatcg aatggtata aagacggca aaccgtatc 
      cgtggcctt taatggtat cacagttaa tcctctacc 
      ctgagagtg ccaatcaaa cggtataca cacagtccg 
      ttacggaag ccgacgctg gaaattaca cttgtttat 
      tgaggaacg actccgtag ttcactctc acacaatcc 
      agctcaaag tcttcgaaa aaattccag acgatccga 
      aaatcacgt acgtgtcaa aggaccacc aggtgccgt 
      tgggccaac agctccgcc tcttctgcg aggcctttg 
      ctggacgag tggatctac cagatgcac acaatgagg 
      ttgtgtggc gcaagtcgg gacataatg gaacgcttg 
      atgacgagc ctcgagtta gacaggaaa aaatctcca 
      gagaggacg ggcaaactt tcggtactt acctgataa 
      tcgacagcg tccggagag aagacttcg gcgagtacg 
      tctgtaaaa taagcaaac ccggaaaaa gcatcgagc 
      agcgggtct ttgtggacg aaaaaatcg aagtggaat 
      atttaaacc ctaatcccg taccagtgg caaagcttc 
      tcctttgcc tgtctgcga ttatttttc tgtgcttgg 
      cggttgtga tcctcaatc ttcagtacg gtttgccgt 
      tgcgggtgc gctttaagg acaccttcg gcgtcgttg 
      aagaatcag acgggaaaa cgaacgacg ttctccttc 
      tgtattcat caaaggaca gcgaactgg ctttgggga 
      cccttttgc caatcttgg aatcaagat acaattaca 
      aatgtgtcc acagggaac tcccctcga gtatcaact 
      actggttcg gggaattgc aaaatgacg ctctaaaga 
      gccgcagaa ttgtaacgc ttctctctc cgccagtgg 
      ttagtgaca actggactt ctgcggcta tttaccaag 
      ctataaaac agctacaag cgttagggt ccaagctga 
      cgattatca cgctgaagg agcttccac gatgcgaga 
      atgaaacga aaaactcgc acggggaga cgctgacca 
      ttttgttga ggacgatca atgtagtgc attgggaga 
      ggacgaagg aggagaaat tttggctta acttgaggc 
      tgcggctgc ctccaaaaa ggcgaaatg aacgactgg 
      tagacggta gaagtcaaa cgcagggta taaattctt 
      caagactga gcaacgact ctgaagaga gttccgatc 
      ttgtgtaaa tatttatta taatttaaa aatgtgttg 
      aattattgt aaccttagt attatattt gttccaact 
      attacactt ataaatata tgcttaaag ttttgtgat 
      tttctaaat acgttgttt tattttgta tttttttat 
      gtaaaataa acgtcgttc gagctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY