Status
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Gene information
General information
TC014284 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8400895..8402459
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014284
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08280

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014284 RA
      MNPFSEFEH VQIEEVCRI CLTKKEQMS LIYETGLAD 
MLLECASLQ VTPDNGLFN FVCQQCQNE VSRWYLFKQ 
QVVRSFEVG KWILERRNK KLENDELTN TIKDEVDIP 
PSVFDVNIA FNTLANNSK DVQSTFLVN ESSNQIVNT 
PTDSQSKKK RTREDLVCK ICHKQCLTT NSYNRHIRT 
HDNSRPFVC SKCDKAFKT TQVLSEHMK RHYDDRRHQ 
CALCSKKFY SKASLNDHL RSHTGEKPF GCEVCGRAF 
GTKAILRQH LGIHTVREK KHQCNICGK FLLSAGSLD 
THVRKHSGI KPFCCPGCD KSFFTKEAM QRHFDAIHD 
TDNNFLCNF CSKVCSTKV HLNNHLKKK HLGLGKPPN 
AVCTECGKQ CGSAAELKV HVRVHTGER PYKCDICNK 
RFIAKGNLN SHKWRHTGR KPFLCNHCG KAFGEKSTL 
KVHERIHTG EQPYKCELC EKAYVQSSA LRTHMKGHE 
KQ
    CDS sequence
    • >TC014284 RA
      atgaacccg ttttcggaa tttgaacac gtacaaatc 
gaagaagta tgccgcatt tgccttaca aagaaagaa 
cagatgagt ttaatttat gaaactggt ttagcagac 
atgcttttg gaatgtgct tcgctccag gtaacacct 
gacaatgga ctttttaat tttgtgtgt cagcaatgc 
caaaacgaa gtcagccgt tggtacctc tttaaacag 
caagtagtt cggtctttt gaagtcggt aaatggata 
ttggagaga agaaacaaa aaactcgaa aatgatgaa 
ttaacaaac acaataaaa gacgaagtg gacattccc 
ccaagtgtg tttgatgta aatatcgct ttcaatact 
ttagccaac aatagtaaa gatgtgcaa agcactttc 
ttggtcaat gagtctagt aatcaaata gtgaacacc 
ccaactgac tctcagtca aaaaagaaa cgaacacgc 
gaagattta gtttgcaaa atttgccac aaacaatgt 
ctcaccacc aattcgtac aatcgccac atcagaacg 
catgataat tcccgaccg ttcgtttgc agcaagtgt 
gataaggcc ttcaagaca acccaagtg ctgtcagaa 
cacatgaaa cggcattac gacgacagg cgccaccag 
tgtgctctt tgcagcaaa aagttttac tcaaaagcg 
agtctaaac gaccactta cggagccac acaggggag 
aaacctttc ggatgcgaa gtctgcgga cgggctttc 
gggaccaag gccatcctc agacaacac ctgggaatt 
cacacagtg cgtgaaaag aaacaccag tgtaacatt 
tgcggcaaa ttcctgctc tcagccggc agtctagac 
acccacgtg aggaaacac tccggcata aaaccgttc 
tgttgcccc ggttgtgac aaaagcttc ttcactaaa 
gaagcaatg caaagacat ttcgacgca atccacgac 
actgacaac aacttcctg tgcaacttc tgctcaaaa 
gtgtgttca acgaaagta catctcaac aaccacttg 
aagaagaag catctaggg ctggggaag cccccgaat 
gctgtgtgc accgagtgt ggcaagcag tgtggttca 
gccgccgaa ctgaaggtc catgttagg gtacacacg 
ggagagagg ccgtacaag tgcgatatt tgcaataag 
aggtttatt gcaaaaggc aatttgaat tcgcacaag 
tggagacac acgggaaga aagccgttt ttgtgtaac 
cactgtggg aaagcgttt ggggaaaag tccacgctc 
aaagtgcac gagaggata cacacgggg gagcagccg 
tacaagtgc gagctctgc gaaaaggcc tatgtgcag 
agcagcgcc ctgaggacg cacatgaaa ggccatgaa 
aaacagtga
    mRNA sequence
    • >TC014284 RA
      cgttgtttg cgatcaatt tttgaacac aacaaaaat 
gttaattct ttaaaaaat gaacccgtt ttcggaatt 
tgaacacgt acaaatcga agaagtatg ccgcatttg 
ccttacaaa gaaagaaca gatgagttt aatttatga 
aactggttt agcagacat gcttttgga atgtgcttc 
gctccaggt aacacctga caatggact ttttaattt 
tgtgtgtca gcaatgcca aaacgaagt cagccgttg 
gtacctctt taaacagca agtagttcg gtcttttga 
agtcggtaa atggatatt ggagagaag aaacaaaaa 
actcgaaaa tgatgaatt aacaaacac aataaaaga 
cgaagtgga cattccccc aagtgtgtt tgatgtaaa 
tatcgcttt caatacttt agccaacaa tagtaaaga 
tgtgcaaag cactttctt ggtcaatga gtctagtaa 
tcaaatagt gaacacccc aactgactc tcagtcaaa 
aaagaaacg aacacgcga agatttagt ttgcaaaat 
ttgccacaa acaatgtct caccaccaa ttcgtacaa 
tcgccacat cagaacgca tgataattc ccgaccgtt 
cgtttgcag caagtgtga taaggcctt caagacaac 
ccaagtgct gtcagaaca catgaaacg gcattacga 
cgacaggcg ccaccagtg tgctctttg cagcaaaaa 
gttttactc aaaagcgag tctaaacga ccacttacg 
gagccacac aggggagaa acctttcgg atgcgaagt 
ctgcggacg ggctttcgg gaccaaggc catcctcag 
acaacacct gggaattca cacagtgcg tgaaaagaa 
acaccagtg taacatttg cggcaaatt cctgctctc 
agccggcag tctagacac ccacgtgag gaaacactc 
cggcataaa accgttctg ttgccccgg ttgtgacaa 
aagcttctt cactaaaga agcaatgca aagacattt 
cgacgcaat ccacgacac tgacaacaa cttcctgtg 
caacttctg ctcaaaagt gtgttcaac gaaagtaca 
tctcaacaa ccacttgaa gaagaagca tctagggct 
ggggaagcc cccgaatgc tgtgtgcac cgagtgtgg 
caagcagtg tggttcagc cgccgaact gaaggtcca 
tgttagggt acacacggg agagaggcc gtacaagtg 
cgatatttg caataagag gtttattgc aaaaggcaa 
tttgaattc gcacaagtg gagacacac gggaagaaa 
gccgttttt gtgtaacca ctgtgggaa agcgtttgg 
ggaaaagtc cacgctcaa agtgcacga gaggataca 
cacggggga gcagccgta caagtgcga gctctgcga 
aaaggccta tgtgcagag cagcgccct gaggacgca 
catgaaagg ccatgaaaa acagtgaag gtttggaga 
ttttataaa tttatttat gaataaata ctagtcaca 
agg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY