General information | |||
TC014284 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 8400895..8402459 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC014284 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08280 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014284 RA
MNPFSEFEH VQIEEVCRI CLTKKEQMS LIYETGLAD MLLECASLQ VTPDNGLFN FVCQQCQNE VSRWYLFKQ QVVRSFEVG KWILERRNK KLENDELTN TIKDEVDIP PSVFDVNIA FNTLANNSK DVQSTFLVN ESSNQIVNT PTDSQSKKK RTREDLVCK ICHKQCLTT NSYNRHIRT HDNSRPFVC SKCDKAFKT TQVLSEHMK RHYDDRRHQ CALCSKKFY SKASLNDHL RSHTGEKPF GCEVCGRAF GTKAILRQH LGIHTVREK KHQCNICGK FLLSAGSLD THVRKHSGI KPFCCPGCD KSFFTKEAM QRHFDAIHD TDNNFLCNF CSKVCSTKV HLNNHLKKK HLGLGKPPN AVCTECGKQ CGSAAELKV HVRVHTGER PYKCDICNK RFIAKGNLN SHKWRHTGR KPFLCNHCG KAFGEKSTL KVHERIHTG EQPYKCELC EKAYVQSSA LRTHMKGHE KQ
- >TC014284 RA
atgaacccg ttttcggaa tttgaacac gtacaaatc gaagaagta tgccgcatt tgccttaca aagaaagaa cagatgagt ttaatttat gaaactggt ttagcagac atgcttttg gaatgtgct tcgctccag gtaacacct gacaatgga ctttttaat tttgtgtgt cagcaatgc caaaacgaa gtcagccgt tggtacctc tttaaacag caagtagtt cggtctttt gaagtcggt aaatggata ttggagaga agaaacaaa aaactcgaa aatgatgaa ttaacaaac acaataaaa gacgaagtg gacattccc ccaagtgtg tttgatgta aatatcgct ttcaatact ttagccaac aatagtaaa gatgtgcaa agcactttc ttggtcaat gagtctagt aatcaaata gtgaacacc ccaactgac tctcagtca aaaaagaaa cgaacacgc gaagattta gtttgcaaa atttgccac aaacaatgt ctcaccacc aattcgtac aatcgccac atcagaacg catgataat tcccgaccg ttcgtttgc agcaagtgt gataaggcc ttcaagaca acccaagtg ctgtcagaa cacatgaaa cggcattac gacgacagg cgccaccag tgtgctctt tgcagcaaa aagttttac tcaaaagcg agtctaaac gaccactta cggagccac acaggggag aaacctttc ggatgcgaa gtctgcgga cgggctttc gggaccaag gccatcctc agacaacac ctgggaatt cacacagtg cgtgaaaag aaacaccag tgtaacatt tgcggcaaa ttcctgctc tcagccggc agtctagac acccacgtg aggaaacac tccggcata aaaccgttc tgttgcccc ggttgtgac aaaagcttc ttcactaaa gaagcaatg caaagacat ttcgacgca atccacgac actgacaac aacttcctg tgcaacttc tgctcaaaa gtgtgttca acgaaagta catctcaac aaccacttg aagaagaag catctaggg ctggggaag cccccgaat gctgtgtgc accgagtgt ggcaagcag tgtggttca gccgccgaa ctgaaggtc catgttagg gtacacacg ggagagagg ccgtacaag tgcgatatt tgcaataag aggtttatt gcaaaaggc aatttgaat tcgcacaag tggagacac acgggaaga aagccgttt ttgtgtaac cactgtggg aaagcgttt ggggaaaag tccacgctc aaagtgcac gagaggata cacacgggg gagcagccg tacaagtgc gagctctgc gaaaaggcc tatgtgcag agcagcgcc ctgaggacg cacatgaaa ggccatgaa aaacagtga
- >TC014284 RA
cgttgtttg cgatcaatt tttgaacac aacaaaaat gttaattct ttaaaaaat gaacccgtt ttcggaatt tgaacacgt acaaatcga agaagtatg ccgcatttg ccttacaaa gaaagaaca gatgagttt aatttatga aactggttt agcagacat gcttttgga atgtgcttc gctccaggt aacacctga caatggact ttttaattt tgtgtgtca gcaatgcca aaacgaagt cagccgttg gtacctctt taaacagca agtagttcg gtcttttga agtcggtaa atggatatt ggagagaag aaacaaaaa actcgaaaa tgatgaatt aacaaacac aataaaaga cgaagtgga cattccccc aagtgtgtt tgatgtaaa tatcgcttt caatacttt agccaacaa tagtaaaga tgtgcaaag cactttctt ggtcaatga gtctagtaa tcaaatagt gaacacccc aactgactc tcagtcaaa aaagaaacg aacacgcga agatttagt ttgcaaaat ttgccacaa acaatgtct caccaccaa ttcgtacaa tcgccacat cagaacgca tgataattc ccgaccgtt cgtttgcag caagtgtga taaggcctt caagacaac ccaagtgct gtcagaaca catgaaacg gcattacga cgacaggcg ccaccagtg tgctctttg cagcaaaaa gttttactc aaaagcgag tctaaacga ccacttacg gagccacac aggggagaa acctttcgg atgcgaagt ctgcggacg ggctttcgg gaccaaggc catcctcag acaacacct gggaattca cacagtgcg tgaaaagaa acaccagtg taacatttg cggcaaatt cctgctctc agccggcag tctagacac ccacgtgag gaaacactc cggcataaa accgttctg ttgccccgg ttgtgacaa aagcttctt cactaaaga agcaatgca aagacattt cgacgcaat ccacgacac tgacaacaa cttcctgtg caacttctg ctcaaaagt gtgttcaac gaaagtaca tctcaacaa ccacttgaa gaagaagca tctagggct ggggaagcc cccgaatgc tgtgtgcac cgagtgtgg caagcagtg tggttcagc cgccgaact gaaggtcca tgttagggt acacacggg agagaggcc gtacaagtg cgatatttg caataagag gtttattgc aaaaggcaa tttgaattc gcacaagtg gagacacac gggaagaaa gccgttttt gtgtaacca ctgtgggaa agcgtttgg ggaaaagtc cacgctcaa agtgcacga gaggataca cacggggga gcagccgta caagtgcga gctctgcga aaaggccta tgtgcagag cagcgccct gaggacgca catgaaagg ccatgaaaa acagtgaag gtttggaga ttttataaa tttatttat gaataaata ctagtcaca agg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
