Status
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Gene information
General information
TC013532 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8335546..8343893
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013532
    iBeetle screen iB_08279

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013532 RA
      MALFNKLFS NRRGTHNKH QENVSEKFF AFSKDQVRV 
      VLFRECDFR GRKLLFDSS SVKKVPINV TKTGEAIKS 
      ENEKYSEIS DGYGYVYTK PEPDFSQLA EMVFGSVAM 
      SFRGTSFKM HSLESPNRL MFTQVFSSP RLANHGRVS 
      ISSRSDAHS LEHSKLDDS GASSTNSMS EHLSVHSTA 
      SDTVLVCRR PQSVPLDVP VSNSVSMSI PSLIGDSGY 
      SYSFNNLTA GPPSSWGSI YGDNGSSKS LSCSSLYKR 
      WLRRTSTSL ENSGQSLAN SVQEGDHVR HHRSSKLGL 
      ALILRLTSG QEEELGQFL MEHISLVES MLWRARQVV 
      EIAYIKSQN FASLMVELA NISAQYLVN LLSGSYLSM 
      NLWLSLSNG CDNYNSISA FSDKSLVWC DRDLSCSTS 
      SVSSCGSAL KEADTSGDF HLFNFAKFL KTDPFNIKK 
      NFFGENRII TAERFVQDF CELLEDIDI KDTNFFMST 
      LLTAVLTYH LGWVNTCLP STGDTSQPR HPFNPLWGE 
      LSDLFGAVG HPTKTAQTI ITGTNKKDL ISKLLVSMT 
      YFIRCCDVD RRVPIRADV HEENKTVEK ICREYSCIP 
      KENYKKYED HLKEIMNCN KEFFAKCHN NNNSCKSGE 
      LEVSFKDSA FIKTHTCLG DLSKLAEED SSECVYPEP 
      NFKPLYPSL RAVKIEESP TDSVSCPPS TDEIVKKVN 
      TLCRVPKSS VLSQLDKQK NYATIVSME KQMRSDDFE 
      PKPPTSDNV VFVLGDNEQ LIGIKKRPS FLDLSKQIR 
      DSDFVENTD KSLVKPSTS WSTRVEKEI GESQKTCDT 
      RKKYPRSQS EPPEDRKLD EAKNQSKYR YSGVKFTLQ 
      QYPQILSNY MKSKNVEIS NLQFGDKSL KLDDLSQLS 
      DTIISKYPE LDEDYDEGE ALQTPSNAS ELEFTSDLF 
      VPSSAKQTK ESNVEPPNQ HVPNTTTNC DEMKTKRKI 
      SNSCSVYKM KVTNLPMPK SKFYKPMIS PIRYTSSLM 
      RAASDSYMP DMVLQGPVV PRQNWEAKL KSDLALASQ 
      HSLIDQPVE EAVAVIANT DTWEVQLMS SHTFLIDKG 
      SSGIRIGMS QLVSNMLES LLQMWKLKV PPHFCLMHI 
      EQTLQELCI RSKALAELL LATEFCNME QLTSALQLE 
      VNDVPLLLA VASTHSPQV TQKYGLSFQ
    CDS sequence
    • >TC013532 RA
      atggcccta ttcaacaaa ctattctcg aaccggagg 
      ggcacacac aataagcac caagagaat gtcagtgag 
      aaatttttt gcatttagt aaggaccaa gtacgagtc 
      gtgttattc agagaatgt gacttccgc ggccgtaag 
      cttttattc gactcaagc tccgtcaaa aaagtaccc 
      atcaatgtt acgaaaaca ggcgaagcc atcaaatcg 
      gaaaacgaa aaatattcc gaaatatcg gacggttac 
      ggctatgtg tacacaaaa cctgaaccc gacttcagc 
      cagcttgcc gagatggtt ttcggttcg gtagcgatg 
      agttttagg ggaacgtcg ttcaaaatg catagcttg 
      gagtctccg aacagacta atgttcaca caggtgttt 
      tctagtcct cgtctcgca aaccatggt cgagtttcc 
      atttctagt cgtagtgat gctcatagc ctcgaacat 
      tccaaatta gacgattcg ggcgccagt agtacgaat 
      tccatgagt gaacacttg tcagtacac agtacagcc 
      tcagacacc gttttagtt tgcagacgg cctcagagc 
      gtcccattg gacgtgcca gtctccaac agtgtatcc 
      atgtcaatc cccagctta atcggcgat tccggctac 
      agttactcg tttaacaac ctaacagca gggccccca 
      tcgtcctgg ggctcaatt tatggtgat aatggtagt 
      tccaaatcg ttgtcttgt agcagcttg tacaaacgt 
      tggttgcgt cggacgagc acatctctg gaaaactct 
      ggccagtct ctggccaat tccgtgcaa gagggggat 
      cacgtgaga caccatcgc agcagcaaa ctgggtctg 
      gccctcatc ctgcggctc acatcagga caggaagag 
      gagctgggc cagttcctg atggagcac atctctctc 
      gtggagtca atgctgtgg cgggcgcgc caagtcgtc 
      gaaatcgcc tacatcaag tcgcagaat ttcgcctcg 
      ttgatggtc gaattggcg aacatctcg gcccagtac 
      ctagtcaat ctgctctct ggttcttac ctttccatg 
      aatttgtgg ctgtcgctg tcaaatggc tgtgataat 
      tacaacagt attagtgca tttagtgat aagagttta 
      gtttggtgt gacagggat ttgagttgc tcgaccagt 
      tcggttagt tcctgtggg agtgcactc aaggaggcg 
      gacactagt ggcgatttt cacttgttc aatttcgcg 
      aagtttcta aagacggac ccgtttaat attaagaag 
      aattttttt ggcgaaaac aggattatt acagccgag 
      aggttcgtg caggatttt tgcgagctg ctggaagat 
      atcgacatt aaggacact aacttcttc atgagtact 
      cttctgacc gcagttctg acatatcac ttggggtgg 
      gtcaacacc tgcttgccc tcgaccggc gacacgtcg 
      cagcccaga caccccttc aaccccttg tggggcgaa 
      ctaagcgat ctcttcggg gccgtgggc cacccgacc 
      aagactgcc caaaccata atcaccgga acgaataag 
      aaagatctg atttcgaag ctgttagtt tcaatgacc 
      tattttatc cgctgctgt gacgtagac aggagagtc 
      ccgatccgt gccgacgtc cacgaggag aacaaaact 
      gtggagaaa atctgtcgg gaatattcg tgcattcca 
      aaagagaat tacaaaaag tacgaggat cacctgaag 
      gagatcatg aactgcaac aaggaattt ttcgcaaag 
      tgtcacaac aacaacaat tcgtgcaaa agtggcgag 
      ttggaggtg tccttcaaa gactctgcg tttattaag 
      acacatacg tgcctaggg gacttgtcc aagttagcc 
      gaagaggat tcctccgag tgtgtgtat ccagaaccg 
      aactttaag cctttgtat ccttcacta cgggctgtg 
      aaaatcgaa gaatcgccc accgactcg gtctcatgt 
      cctccaagc accgacgaa attgtgaaa aaagtcaac 
      acgttgtgt cgcgtcccc aaaagctcg gtcctctcc 
      cagttggat aaacaaaaa aattacgcc acgatcgtc 
      agcatggaa aaacaaatg cgaagcgat gacttcgaa 
      ccgaaaccg cccacttcg gacaacgtc gtgttcgtg 
      ttaggcgac aacgaacag ctaatcggg ataaagaag 
      cgcccctca ttcttagac ctgtccaag caaattcgc 
      gactcagac tttgtggaa aacacggac aagtcgttg 
      gtcaaaccg agcacctcg tggtccact cgcgtggag 
      aaggagatc ggggaatca cagaaaacg tgcgacact 
      cggaaaaaa tacccgcgc tcgcagagt gaacccccg 
      gaagaccgg aagctggac gaggcgaaa aatcagtcg 
      aagtatcgc tattccggg gtcaaattc accctacag 
      cagtacccc cagattttg tcaaattac atgaagagc 
      aaaaacgtg gaaatctcc aatttgcag ttcggcgat 
      aagagtttg aagcttgac gatttgtcc caactgagc 
      gacacgatt atttcgaag tatcccgag ctggacgag 
      gactacgac gagggcgag gccctgcag acgccctcc 
      aacgcctcc gagctggag ttcacgagt gatttgttc 
      gtcccctcg tccgcgaaa cagacgaag gagtcgaac 
      gtggagccg cccaatcag catgtgcca aatacgacc 
      acgaattgt gatgagatg aagactaag cgtaaaatt 
      tcgaatagt tgctcagtt tataagatg aaagttacg 
      aatctgccg atgccaaag tctaagttt tacaagccg 
      atgattagt cctatcaga tatacgtcg tcactgatg 
      agggcagct agtgatagt tacatgccg gacatggtg 
      ttgcaagga ccagtggtg ccgaggcag aactgggag 
      gcgaaactg aagagtgat ttggctttg gcgagccaa 
      cattccctc attgatcag ccggttgag gaggctgtg 
      gccgttatt gccaacacg gacacttgg gaggtgcag 
      ctcatgtcg agtcacacg ttcctcatt gacaaggga 
      tcgtcaggg atcaggata ggcatgtct cagttggtc 
      tcaaatatg ttggagtcg ttgctccag atgtggaaa 
      ctcaaagtc cccccacat ttctgtctc atgcacatt 
      gagcagacg cttcaagaa ctttgcata cgatccaaa 
      gctctcgcc gagttgctt ttagccaca gaattctgc 
      aacatggaa cagctgaca tccgctctc cagttggaa 
      gtcaacgac gtacctctt ttactggct gtggcttcg 
      acgcattcg ccacaagtg acacaaaaa tacggcctc 
      agttttcag taa
    mRNA sequence
    • >TC013532 RA
      caaaataaa aatgacaac acatcctct catactgaa 
      aagggttga cagcagtgt tgacacttg tcaaatttt 
      tctctgaca gacgcactc ttgatgcct taacaagtt 
      tccgatttt ccaaaaaaa cgttttttg gtggaattc 
      ttcaagcct tgtgacccc cagccatgc aaaatcagt 
      atccttcgc ccgagccac ttcctggtg gctggagcc 
      ccgccaact agcgcaatt actccaagt gacggcctg 
      gcaacagcg cttccctcc attccagtt tccaacatt 
      tttatcata cccagaggt tatctatta ccacaccga 
      aaagaagat gttttttaa gcagtttca gcccgttaa 
      ttgaataat attccaaag gcaaatcgc gcagcactc 
      gcgtcatgt gctaacgcg aaatccctg cgcatagaa 
      gtgaactcg gtgacccgt cggtgacca aatcatatg 
      acttgaatg cacgtgata tttactgtg tgagtggac 
      tttttctgg catcaggca aggcgaaat tcgtgctgc 
      tcgtaaatg attctgatc agtgagggc tcgctgata 
      tggccctat tcaacaaac tattctcga accggaggg 
      gcacacaca ataagcacc aagagaatg tcagtgaga 
      aattttttg catttagta aggaccaag tacgagtcg 
      tgttattca gagaatgtg acttccgcg gccgtaagc 
      ttttattcg actcaagct ccgtcaaaa aagtaccca 
      tcaatgtta cgaaaacag gcgaagcca tcaaatcgg 
      aaaacgaaa aatattccg aaatatcgg acggttacg 
      gctatgtgt acacaaaac ctgaacccg acttcagcc 
      agcttgccg agatggttt tcggttcgg tagcgatga 
      gttttaggg gaacgtcgt tcaaaatgc atagcttgg 
      agtctccga acagactaa tgttcacac aggtgtttt 
      ctagtcctc gtctcgcaa accatggtc gagtttcca 
      tttctagtc gtagtgatg ctcatagcc tcgaacatt 
      ccaaattag acgattcgg gcgccagta gtacgaatt 
      ccatgagtg aacacttgt cagtacaca gtacagcct 
      cagacaccg ttttagttt gcagacggc ctcagagcg 
      tcccattgg acgtgccag tctccaaca gtgtatcca 
      tgtcaatcc ccagcttaa tcggcgatt ccggctaca 
      gttactcgt ttaacaacc taacagcag ggcccccat 
      cgtcctggg gctcaattt atggtgata atggtagtt 
      ccaaatcgt tgtcttgta gcagcttgt acaaacgtt 
      ggttgcgtc ggacgagca catctctgg aaaactctg 
      gccagtctc tggccaatt ccgtgcaag agggggatc 
      acgtgagac accatcgca gcagcaaac tgggtctgg 
      ccctcatcc tgcggctca catcaggac aggaagagg 
      agctgggcc agttcctga tggagcaca tctctctcg 
      tggagtcaa tgctgtggc gggcgcgcc aagtcgtcg 
      aaatcgcct acatcaagt cgcagaatt tcgcctcgt 
      tgatggtcg aattggcga acatctcgg cccagtacc 
      tagtcaatc tgctctctg gttcttacc tttccatga 
      atttgtggc tgtcgctgt caaatggct gtgataatt 
      acaacagta ttagtgcat ttagtgata agagtttag 
      tttggtgtg acagggatt tgagttgct cgaccagtt 
      cggttagtt cctgtggga gtgcactca aggaggcgg 
      acactagtg gcgattttc acttgttca atttcgcga 
      agtttctaa agacggacc cgtttaata ttaagaaga 
      atttttttg gcgaaaaca ggattatta cagccgaga 
      ggttcgtgc aggattttt gcgagctgc tggaagata 
      tcgacatta aggacacta acttcttca tgagtactc 
      ttctgaccg cagttctga catatcact tggggtggg 
      tcaacacct gcttgccct cgaccggcg acacgtcgc 
      agcccagac accccttca accccttgt ggggcgaac 
      taagcgatc tcttcgggg ccgtgggcc acccgacca 
      agactgccc aaaccataa tcaccggaa cgaataaga 
      aagatctga tttcgaagc tgttagttt caatgacct 
      attttatcc gctgctgtg acgtagaca ggagagtcc 
      cgatccgtg ccgacgtcc acgaggaga acaaaactg 
      tggagaaaa tctgtcggg aatattcgt gcattccaa 
      aagagaatt acaaaaagt acgaggatc acctgaagg 
      agatcatga actgcaaca aggaatttt tcgcaaagt 
      gtcacaaca acaacaatt cgtgcaaaa gtggcgagt 
      tggaggtgt ccttcaaag actctgcgt ttattaaga 
      cacatacgt gcctagggg acttgtcca agttagccg 
      aagaggatt cctccgagt gtgtgtatc cagaaccga 
      actttaagc ctttgtatc cttcactac gggctgtga 
      aaatcgaag aatcgccca ccgactcgg tctcatgtc 
      ctccaagca ccgacgaaa ttgtgaaaa aagtcaaca 
      cgttgtgtc gcgtcccca aaagctcgg tcctctccc 
      agttggata aacaaaaaa attacgcca cgatcgtca 
      gcatggaaa aacaaatgc gaagcgatg acttcgaac 
      cgaaaccgc ccacttcgg acaacgtcg tgttcgtgt 
      taggcgaca acgaacagc taatcggga taaagaagc 
      gcccctcat tcttagacc tgtccaagc aaattcgcg 
      actcagact ttgtggaaa acacggaca agtcgttgg 
      tcaaaccga gcacctcgt ggtccactc gcgtggaga 
      aggagatcg gggaatcac agaaaacgt gcgacactc 
      ggaaaaaat acccgcgct cgcagagtg aacccccgg 
      aagaccgga agctggacg aggcgaaaa atcagtcga 
      agtatcgct attccgggg tcaaattca ccctacagc 
      agtaccccc agattttgt caaattaca tgaagagca 
      aaaacgtgg aaatctcca atttgcagt tcggcgata 
      agagtttga agcttgacg atttgtccc aactgagcg 
      acacgatta tttcgaagt atcccgagc tggacgagg 
      actacgacg agggcgagg ccctgcaga cgccctcca 
      acgcctccg agctggagt tcacgagtg atttgttcg 
      tcccctcgt ccgcgaaac agacgaagg agtcgaacg 
      tggagccgc ccaatcagc atgtgccaa atacgacca 
      cgaattgtg atgagatga agactaagc gtaaaattt 
      cgaatagtt gctcagttt ataagatga aagttacga 
      atctgccga tgccaaagt ctaagtttt acaagccga 
      tgattagtc ctatcagat atacgtcgt cactgatga 
      gggcagcta gtgatagtt acatgccgg acatggtgt 
      tgcaaggac cagtggtgc cgaggcaga actgggagg 
      cgaaactga agagtgatt tggctttgg cgagccaac 
      attccctca ttgatcagc cggttgagg aggctgtgg 
      ccgttattg ccaacacgg acacttggg aggtgcagc 
      tcatgtcga gtcacacgt tcctcattg acaagggat 
      cgtcaggga tcaggatag gcatgtctc agttggtct 
      caaatatgt tggagtcgt tgctccaga tgtggaaac 
      tcaaagtcc ccccacatt tctgtctca tgcacattg 
      agcagacgc ttcaagaac tttgcatac gatccaaag 
      ctctcgccg agttgcttt tagccacag aattctgca 
      acatggaac agctgacat ccgctctcc agttggaag 
      tcaacgacg tacctcttt tactggctg tggcttcga 
      cgcattcgc cacaagtga cacaaaaat acggcctca 
      gttttcagt aattttatt gtcagtata atctattct 
      ttatattgt atttatttt gtatatagt ggcattttt 
      taatttata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY