| General information | |||
| TC033108
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 8087930..8090880 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033108 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08275 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033108 RA
MIPDYASIS HAMFRYPDI GTSQMGSHI TILRPRDIV STPSDVIST AASYFNSND QNESTWTRR AKFFFRILS FCLGPANIW RFPQQSFIY GNATYMYLY ILYSWIIGV SLLVFEVSL SQYFMKGFA KLFTLCPIM EGITLSMAF YCIIATLLY SMICVYSFV YVFTGFHPK MPHSYCSEP IKTYGCKTN YTNFTDSPE FIFFYNYVE RYRDADANY YNWRNAVLL VFIWLIILF ITWFSILRY HWVVIIHSV SVFLILIYA ATIGLVVDS KNGVFPGIT RMFVPTTND ILNIRTWIY PLEQSVVSL GVGAGPLIT IWSVSKLKY WTPCDTLTI NVITFICSI LSAIVFFTS AGLLADQKK TIPEKQEEL HVHVLYTPL FNNMKGNGA SNFSVILYF YVLFCSCLY SLLEYVQIL LLYISSYVL IDRRRRKII IVATCLFLC AFSTIIGDF KWGFAIIMS INKNFIATA LLIILTFEL LSVSYCYGF RNFCDDIEF MLGFVPNYF FQVTWLLLP VVFSVFSVV SLYLKFHEI ENNVDVVML TLIILFIIM PVVLIATIK CIVGIRDMG IRRVLNPRR NESDWDTDS VRISPTSSI LDIHKDSLH NLRGEVGSE YVSTQNISI ASETDYASM KRRQSKATG
- >TC033108 RA
atgatcccg gactatgcg agcatatct cacgcaatg ttccggtat ccggacatc ggaacgtct caaatgggg tcccacatt acgattctg agaccccgg gacattgtc tccaccccc tccgatgtg atttccaca gctgcttct tatttcaac tcaaatgat cagaacgaa tcaacttgg acacgcaga gccaaattc tttttccga attttgagt ttttgttta ggcccagct aacatttgg cggttcccg cagcagtcg tttatttac gggaatgcg acatacatg tacttgtat attttgtac agctggata attggggtg tcattgctg gtgtttgag gtcagtctc agtcagtat tttatgaag ggtttcgcc aaacttttc actctttgc cccatcatg gaaggaata acactttcg atggcattt tactgcata attgctact ttgttgtac agtatgata tgtgtatac tctttcgtg tatgtcttt accggattt cacccaaaa atgccccat tcgtactgt tccgaaccc attaaaaca tacggttgt aaaacaaat tatacgaac tttaccgac tcccccgaa ttcatcttc ttctacaat tacgtggaa aggtacaga gacgcagat gcaaattac tacaattgg cgaaacgct gtccttttg gtttttatt tggcttata attctcttc atcacttgg ttcagtatt ttgcgatac cattgggtc gtaataata cacagcgtt tcagttttt ttgatacta atctacgcc gcaactatt ggtctagta gtggattca aagaatggt gtttttccg ggaatcact cgaatgttt gttccgacc acaaatgat atcctcaac attcgaact tggatatat cccttggaa caaagtgtt gtaagtctt ggtgtaggt gcaggtccc ttgatcaca atatggtct gtctctaaa ctaaaatac tggactcct tgtgacact ctaacgata aatgtgatc acatttatt tgtagtata ttatcagcg attgttttc ttcacttct gcaggactt ttagccgat cagaagaaa acaatccca gaaaaacag gaagaactt cacgttcat gttttgtac actccccta ttcaacaac atgaaggga aatggcgca agtaacttc agcgtgatt ctttacttc tatgtgtta ttttgtagc tgtttatac tctttgcta gaatatgtc caaattttg cttctgtac ataagtagt tacgttcta atcgatagg agaagacgc aaaataatt atagttgct acctgttta tttctgtgc gcattttcg acgattatt ggtgatttc aagtggggt ttcgctatt attatgtca attaataag aactttata gcaactgct ttacttata attctcact tttgagcta ctatcagtg tcatattgt tacggtttt cggaatttt tgcgacgat attgaattt atgctaggt tttgttcct aactacttt ttccaagtg acgtggctg ctgttacca gtagtgttt tctgtattt tctgtagtg tccctttac ctcaaattt cacgagatc gaaaacaac gttgacgta gtaatgttg actttaata atattattt attataatg ccagtggtt ttaattgcc actatcaag tgcattgtt ggaataaga gacatgggc ataagaagg gtgttaaat cctcgtagg aacgagtca gattgggac actgattcg gttcgtatt tcaccgaca agttcaata ttagatatt cataaagat tccctccat aatctgcga ggtgaagtc ggtagtgaa tacgtatcg actcagaat atttcaatc gcgtcggag acggattat gccagcatg aaaagaaga cagagcaaa gcgactggc tag
- >TC033108 RA
tgctcccag aaaaatatt catttggtt gcacggtgt cattgcacg tgtcatttt tgaaagttg aaatttgca aaatgatcc cggactatg cgagcatat ctcacgcaa tgttccggt atccggaca tcggaacgt ctcaaatgg ggtcccaca ttacgattc tgagacccc gggacattg tctccaccc cctccgatg tgatttcca cagctgctt cttatttca actcaaatg atcagaacg aatcaactt ggacacgca gagccaaat tctttttcc gaattttga gtttttgtt taggcccag ctaacattt ggcggttcc cgcagcagt cgtttattt acgggaatg cgacataca tgtacttgt atattttgt acagctgga taattgggg tgtcattgc tggtgtttg aggtcagtc tcagtcagt attttatga agggtttcg ccaaacttt tcactcttt gccccatca tggaaggaa taacacttt cgatggcat tttactgca taattgcta ctttgttgt acagtatga tatgtgtat actctttcg tgtatgtct ttaccggat ttcacccaa aaatgcccc attcgtact gttccgaac ccattaaaa catacggtt gtaaaacaa attatacga actttaccg actcccccg aattcatct tcttctaca attacgtgg aaaggtaca gagacgcag atgcaaatt actacaatt ggcgaaacg ctgtccttt tggttttta tttggctta taattctct tcatcactt ggttcagta ttttgcgat accattggg tcgtaataa tacacagcg tttcagttt ttttgatac taatctacg ccgcaacta ttggtctag tagtggatt caaagaatg gtgtttttc cgggaatca ctcgaatgt ttgttccga ccacaaatg atatcctca acattcgaa cttggatat atcccttgg aacaaagtg ttgtaagtc ttggtgtag gtgcaggtc ccttgatca caatatggt ctgtctcta aactaaaat actggactc cttgtgaca ctctaacga taaatgtga tcacattta tttgtagta tattatcag cgattgttt tcttcactt ctgcaggac ttttagccg atcagaaga aaacaatcc cagaaaaac aggaagaac ttcacgttc atgttttgt acactcccc tattcaaca acatgaagg gaaatggcg caagtaact tcagcgtga ttctttact tctatgtgt tattttgta gctgtttat actctttgc tagaatatg tccaaattt tgcttctgt acataagta gttacgttc taatcgata ggagaagac gcaaaataa ttatagttg ctacctgtt tatttctgt gcgcatttt cgacgatta ttggtgatt tcaagtggg gtttcgcta ttattatgt caattaata agaacttta tagcaactg ctttactta taattctca cttttgagc tactatcag tgtcatatt gttacggtt ttcggaatt tttgcgacg atattgaat ttatgctag gttttgttc ctaactact ttttccaag tgacgtggc tgctgttac cagtagtgt tttctgtat tttctgtag tgtcccttt acctcaaat ttcacgaga tcgaaaaca acgttgacg tagtaatgt tgactttaa taatattat ttattataa tgccagtgg ttttaattg ccactatca agtgcattg ttggaataa gagacatgg gcataagaa gggtgttaa atcctcgta ggaacgagt cagattggg acactgatt cggttcgta tttcaccga caagttcaa tattagata ttcataaag attccctcc ataatctgc gaggtgaag tcggtagtg aatacgtat cgactcaga atatttcaa tcgcgtcgg agacggatt atgccagca tgaaaagaa gacagagca aagcgactg gctagagaa ttgttttta ataaagttc gtttcatga a
