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Gene information
General information
TC033108
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014261 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8087930..8090880
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033108
    iBeetle screen iB_08275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033108 RA
      MIPDYASIS HAMFRYPDI GTSQMGSHI TILRPRDIV 
STPSDVIST AASYFNSND QNESTWTRR AKFFFRILS 
FCLGPANIW RFPQQSFIY GNATYMYLY ILYSWIIGV 
SLLVFEVSL SQYFMKGFA KLFTLCPIM EGITLSMAF 
YCIIATLLY SMICVYSFV YVFTGFHPK MPHSYCSEP 
IKTYGCKTN YTNFTDSPE FIFFYNYVE RYRDADANY 
YNWRNAVLL VFIWLIILF ITWFSILRY HWVVIIHSV 
SVFLILIYA ATIGLVVDS KNGVFPGIT RMFVPTTND 
ILNIRTWIY PLEQSVVSL GVGAGPLIT IWSVSKLKY 
WTPCDTLTI NVITFICSI LSAIVFFTS AGLLADQKK 
TIPEKQEEL HVHVLYTPL FNNMKGNGA SNFSVILYF 
YVLFCSCLY SLLEYVQIL LLYISSYVL IDRRRRKII 
IVATCLFLC AFSTIIGDF KWGFAIIMS INKNFIATA 
LLIILTFEL LSVSYCYGF RNFCDDIEF MLGFVPNYF 
FQVTWLLLP VVFSVFSVV SLYLKFHEI ENNVDVVML 
TLIILFIIM PVVLIATIK CIVGIRDMG IRRVLNPRR 
NESDWDTDS VRISPTSSI LDIHKDSLH NLRGEVGSE 
YVSTQNISI ASETDYASM KRRQSKATG
    CDS sequence
    • >TC033108 RA
      atgatcccg gactatgcg agcatatct cacgcaatg 
ttccggtat ccggacatc ggaacgtct caaatgggg 
tcccacatt acgattctg agaccccgg gacattgtc 
tccaccccc tccgatgtg atttccaca gctgcttct 
tatttcaac tcaaatgat cagaacgaa tcaacttgg 
acacgcaga gccaaattc tttttccga attttgagt 
ttttgttta ggcccagct aacatttgg cggttcccg 
cagcagtcg tttatttac gggaatgcg acatacatg 
tacttgtat attttgtac agctggata attggggtg 
tcattgctg gtgtttgag gtcagtctc agtcagtat 
tttatgaag ggtttcgcc aaacttttc actctttgc 
cccatcatg gaaggaata acactttcg atggcattt 
tactgcata attgctact ttgttgtac agtatgata 
tgtgtatac tctttcgtg tatgtcttt accggattt 
cacccaaaa atgccccat tcgtactgt tccgaaccc 
attaaaaca tacggttgt aaaacaaat tatacgaac 
tttaccgac tcccccgaa ttcatcttc ttctacaat 
tacgtggaa aggtacaga gacgcagat gcaaattac 
tacaattgg cgaaacgct gtccttttg gtttttatt 
tggcttata attctcttc atcacttgg ttcagtatt 
ttgcgatac cattgggtc gtaataata cacagcgtt 
tcagttttt ttgatacta atctacgcc gcaactatt 
ggtctagta gtggattca aagaatggt gtttttccg 
ggaatcact cgaatgttt gttccgacc acaaatgat 
atcctcaac attcgaact tggatatat cccttggaa 
caaagtgtt gtaagtctt ggtgtaggt gcaggtccc 
ttgatcaca atatggtct gtctctaaa ctaaaatac 
tggactcct tgtgacact ctaacgata aatgtgatc 
acatttatt tgtagtata ttatcagcg attgttttc 
ttcacttct gcaggactt ttagccgat cagaagaaa 
acaatccca gaaaaacag gaagaactt cacgttcat 
gttttgtac actccccta ttcaacaac atgaaggga 
aatggcgca agtaacttc agcgtgatt ctttacttc 
tatgtgtta ttttgtagc tgtttatac tctttgcta 
gaatatgtc caaattttg cttctgtac ataagtagt 
tacgttcta atcgatagg agaagacgc aaaataatt 
atagttgct acctgttta tttctgtgc gcattttcg 
acgattatt ggtgatttc aagtggggt ttcgctatt 
attatgtca attaataag aactttata gcaactgct 
ttacttata attctcact tttgagcta ctatcagtg 
tcatattgt tacggtttt cggaatttt tgcgacgat 
attgaattt atgctaggt tttgttcct aactacttt 
ttccaagtg acgtggctg ctgttacca gtagtgttt 
tctgtattt tctgtagtg tccctttac ctcaaattt 
cacgagatc gaaaacaac gttgacgta gtaatgttg 
actttaata atattattt attataatg ccagtggtt 
ttaattgcc actatcaag tgcattgtt ggaataaga 
gacatgggc ataagaagg gtgttaaat cctcgtagg 
aacgagtca gattgggac actgattcg gttcgtatt 
tcaccgaca agttcaata ttagatatt cataaagat 
tccctccat aatctgcga ggtgaagtc ggtagtgaa 
tacgtatcg actcagaat atttcaatc gcgtcggag 
acggattat gccagcatg aaaagaaga cagagcaaa 
gcgactggc tag
    mRNA sequence
    • >TC033108 RA
      tgctcccag aaaaatatt catttggtt gcacggtgt 
cattgcacg tgtcatttt tgaaagttg aaatttgca 
aaatgatcc cggactatg cgagcatat ctcacgcaa 
tgttccggt atccggaca tcggaacgt ctcaaatgg 
ggtcccaca ttacgattc tgagacccc gggacattg 
tctccaccc cctccgatg tgatttcca cagctgctt 
cttatttca actcaaatg atcagaacg aatcaactt 
ggacacgca gagccaaat tctttttcc gaattttga 
gtttttgtt taggcccag ctaacattt ggcggttcc 
cgcagcagt cgtttattt acgggaatg cgacataca 
tgtacttgt atattttgt acagctgga taattgggg 
tgtcattgc tggtgtttg aggtcagtc tcagtcagt 
attttatga agggtttcg ccaaacttt tcactcttt 
gccccatca tggaaggaa taacacttt cgatggcat 
tttactgca taattgcta ctttgttgt acagtatga 
tatgtgtat actctttcg tgtatgtct ttaccggat 
ttcacccaa aaatgcccc attcgtact gttccgaac 
ccattaaaa catacggtt gtaaaacaa attatacga 
actttaccg actcccccg aattcatct tcttctaca 
attacgtgg aaaggtaca gagacgcag atgcaaatt 
actacaatt ggcgaaacg ctgtccttt tggttttta 
tttggctta taattctct tcatcactt ggttcagta 
ttttgcgat accattggg tcgtaataa tacacagcg 
tttcagttt ttttgatac taatctacg ccgcaacta 
ttggtctag tagtggatt caaagaatg gtgtttttc 
cgggaatca ctcgaatgt ttgttccga ccacaaatg 
atatcctca acattcgaa cttggatat atcccttgg 
aacaaagtg ttgtaagtc ttggtgtag gtgcaggtc 
ccttgatca caatatggt ctgtctcta aactaaaat 
actggactc cttgtgaca ctctaacga taaatgtga 
tcacattta tttgtagta tattatcag cgattgttt 
tcttcactt ctgcaggac ttttagccg atcagaaga 
aaacaatcc cagaaaaac aggaagaac ttcacgttc 
atgttttgt acactcccc tattcaaca acatgaagg 
gaaatggcg caagtaact tcagcgtga ttctttact 
tctatgtgt tattttgta gctgtttat actctttgc 
tagaatatg tccaaattt tgcttctgt acataagta 
gttacgttc taatcgata ggagaagac gcaaaataa 
ttatagttg ctacctgtt tatttctgt gcgcatttt 
cgacgatta ttggtgatt tcaagtggg gtttcgcta 
ttattatgt caattaata agaacttta tagcaactg 
ctttactta taattctca cttttgagc tactatcag 
tgtcatatt gttacggtt ttcggaatt tttgcgacg 
atattgaat ttatgctag gttttgttc ctaactact 
ttttccaag tgacgtggc tgctgttac cagtagtgt 
tttctgtat tttctgtag tgtcccttt acctcaaat 
ttcacgaga tcgaaaaca acgttgacg tagtaatgt 
tgactttaa taatattat ttattataa tgccagtgg 
ttttaattg ccactatca agtgcattg ttggaataa 
gagacatgg gcataagaa gggtgttaa atcctcgta 
ggaacgagt cagattggg acactgatt cggttcgta 
tttcaccga caagttcaa tattagata ttcataaag 
attccctcc ataatctgc gaggtgaag tcggtagtg 
aatacgtat cgactcaga atatttcaa tcgcgtcgg 
agacggatt atgccagca tgaaaagaa gacagagca 
aagcgactg gctagagaa ttgttttta ataaagttc 
gtttcatga a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY