Status
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Gene information
General information
TC033108
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014261
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8087930..8090880
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033108
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033108 RA
      MIPDYASIS HAMFRYPDI GTSQMGSHI TILRPRDIV 
      STPSDVIST AASYFNSND QNESTWTRR AKFFFRILS 
      FCLGPANIW RFPQQSFIY GNATYMYLY ILYSWIIGV 
      SLLVFEVSL SQYFMKGFA KLFTLCPIM EGITLSMAF 
      YCIIATLLY SMICVYSFV YVFTGFHPK MPHSYCSEP 
      IKTYGCKTN YTNFTDSPE FIFFYNYVE RYRDADANY 
      YNWRNAVLL VFIWLIILF ITWFSILRY HWVVIIHSV 
      SVFLILIYA ATIGLVVDS KNGVFPGIT RMFVPTTND 
      ILNIRTWIY PLEQSVVSL GVGAGPLIT IWSVSKLKY 
      WTPCDTLTI NVITFICSI LSAIVFFTS AGLLADQKK 
      TIPEKQEEL HVHVLYTPL FNNMKGNGA SNFSVILYF 
      YVLFCSCLY SLLEYVQIL LLYISSYVL IDRRRRKII 
      IVATCLFLC AFSTIIGDF KWGFAIIMS INKNFIATA 
      LLIILTFEL LSVSYCYGF RNFCDDIEF MLGFVPNYF 
      FQVTWLLLP VVFSVFSVV SLYLKFHEI ENNVDVVML 
      TLIILFIIM PVVLIATIK CIVGIRDMG IRRVLNPRR 
      NESDWDTDS VRISPTSSI LDIHKDSLH NLRGEVGSE 
      YVSTQNISI ASETDYASM KRRQSKATG
    CDS sequence
    • >TC033108 RA
      atgatcccg gactatgcg agcatatct cacgcaatg 
      ttccggtat ccggacatc ggaacgtct caaatgggg 
      tcccacatt acgattctg agaccccgg gacattgtc 
      tccaccccc tccgatgtg atttccaca gctgcttct 
      tatttcaac tcaaatgat cagaacgaa tcaacttgg 
      acacgcaga gccaaattc tttttccga attttgagt 
      ttttgttta ggcccagct aacatttgg cggttcccg 
      cagcagtcg tttatttac gggaatgcg acatacatg 
      tacttgtat attttgtac agctggata attggggtg 
      tcattgctg gtgtttgag gtcagtctc agtcagtat 
      tttatgaag ggtttcgcc aaacttttc actctttgc 
      cccatcatg gaaggaata acactttcg atggcattt 
      tactgcata attgctact ttgttgtac agtatgata 
      tgtgtatac tctttcgtg tatgtcttt accggattt 
      cacccaaaa atgccccat tcgtactgt tccgaaccc 
      attaaaaca tacggttgt aaaacaaat tatacgaac 
      tttaccgac tcccccgaa ttcatcttc ttctacaat 
      tacgtggaa aggtacaga gacgcagat gcaaattac 
      tacaattgg cgaaacgct gtccttttg gtttttatt 
      tggcttata attctcttc atcacttgg ttcagtatt 
      ttgcgatac cattgggtc gtaataata cacagcgtt 
      tcagttttt ttgatacta atctacgcc gcaactatt 
      ggtctagta gtggattca aagaatggt gtttttccg 
      ggaatcact cgaatgttt gttccgacc acaaatgat 
      atcctcaac attcgaact tggatatat cccttggaa 
      caaagtgtt gtaagtctt ggtgtaggt gcaggtccc 
      ttgatcaca atatggtct gtctctaaa ctaaaatac 
      tggactcct tgtgacact ctaacgata aatgtgatc 
      acatttatt tgtagtata ttatcagcg attgttttc 
      ttcacttct gcaggactt ttagccgat cagaagaaa 
      acaatccca gaaaaacag gaagaactt cacgttcat 
      gttttgtac actccccta ttcaacaac atgaaggga 
      aatggcgca agtaacttc agcgtgatt ctttacttc 
      tatgtgtta ttttgtagc tgtttatac tctttgcta 
      gaatatgtc caaattttg cttctgtac ataagtagt 
      tacgttcta atcgatagg agaagacgc aaaataatt 
      atagttgct acctgttta tttctgtgc gcattttcg 
      acgattatt ggtgatttc aagtggggt ttcgctatt 
      attatgtca attaataag aactttata gcaactgct 
      ttacttata attctcact tttgagcta ctatcagtg 
      tcatattgt tacggtttt cggaatttt tgcgacgat 
      attgaattt atgctaggt tttgttcct aactacttt 
      ttccaagtg acgtggctg ctgttacca gtagtgttt 
      tctgtattt tctgtagtg tccctttac ctcaaattt 
      cacgagatc gaaaacaac gttgacgta gtaatgttg 
      actttaata atattattt attataatg ccagtggtt 
      ttaattgcc actatcaag tgcattgtt ggaataaga 
      gacatgggc ataagaagg gtgttaaat cctcgtagg 
      aacgagtca gattgggac actgattcg gttcgtatt 
      tcaccgaca agttcaata ttagatatt cataaagat 
      tccctccat aatctgcga ggtgaagtc ggtagtgaa 
      tacgtatcg actcagaat atttcaatc gcgtcggag 
      acggattat gccagcatg aaaagaaga cagagcaaa 
      gcgactggc tag
    mRNA sequence
    • >TC033108 RA
      tgctcccag aaaaatatt catttggtt gcacggtgt 
      cattgcacg tgtcatttt tgaaagttg aaatttgca 
      aaatgatcc cggactatg cgagcatat ctcacgcaa 
      tgttccggt atccggaca tcggaacgt ctcaaatgg 
      ggtcccaca ttacgattc tgagacccc gggacattg 
      tctccaccc cctccgatg tgatttcca cagctgctt 
      cttatttca actcaaatg atcagaacg aatcaactt 
      ggacacgca gagccaaat tctttttcc gaattttga 
      gtttttgtt taggcccag ctaacattt ggcggttcc 
      cgcagcagt cgtttattt acgggaatg cgacataca 
      tgtacttgt atattttgt acagctgga taattgggg 
      tgtcattgc tggtgtttg aggtcagtc tcagtcagt 
      attttatga agggtttcg ccaaacttt tcactcttt 
      gccccatca tggaaggaa taacacttt cgatggcat 
      tttactgca taattgcta ctttgttgt acagtatga 
      tatgtgtat actctttcg tgtatgtct ttaccggat 
      ttcacccaa aaatgcccc attcgtact gttccgaac 
      ccattaaaa catacggtt gtaaaacaa attatacga 
      actttaccg actcccccg aattcatct tcttctaca 
      attacgtgg aaaggtaca gagacgcag atgcaaatt 
      actacaatt ggcgaaacg ctgtccttt tggttttta 
      tttggctta taattctct tcatcactt ggttcagta 
      ttttgcgat accattggg tcgtaataa tacacagcg 
      tttcagttt ttttgatac taatctacg ccgcaacta 
      ttggtctag tagtggatt caaagaatg gtgtttttc 
      cgggaatca ctcgaatgt ttgttccga ccacaaatg 
      atatcctca acattcgaa cttggatat atcccttgg 
      aacaaagtg ttgtaagtc ttggtgtag gtgcaggtc 
      ccttgatca caatatggt ctgtctcta aactaaaat 
      actggactc cttgtgaca ctctaacga taaatgtga 
      tcacattta tttgtagta tattatcag cgattgttt 
      tcttcactt ctgcaggac ttttagccg atcagaaga 
      aaacaatcc cagaaaaac aggaagaac ttcacgttc 
      atgttttgt acactcccc tattcaaca acatgaagg 
      gaaatggcg caagtaact tcagcgtga ttctttact 
      tctatgtgt tattttgta gctgtttat actctttgc 
      tagaatatg tccaaattt tgcttctgt acataagta 
      gttacgttc taatcgata ggagaagac gcaaaataa 
      ttatagttg ctacctgtt tatttctgt gcgcatttt 
      cgacgatta ttggtgatt tcaagtggg gtttcgcta 
      ttattatgt caattaata agaacttta tagcaactg 
      ctttactta taattctca cttttgagc tactatcag 
      tgtcatatt gttacggtt ttcggaatt tttgcgacg 
      atattgaat ttatgctag gttttgttc ctaactact 
      ttttccaag tgacgtggc tgctgttac cagtagtgt 
      tttctgtat tttctgtag tgtcccttt acctcaaat 
      ttcacgaga tcgaaaaca acgttgacg tagtaatgt 
      tgactttaa taatattat ttattataa tgccagtgg 
      ttttaattg ccactatca agtgcattg ttggaataa 
      gagacatgg gcataagaa gggtgttaa atcctcgta 
      ggaacgagt cagattggg acactgatt cggttcgta 
      tttcaccga caagttcaa tattagata ttcataaag 
      attccctcc ataatctgc gaggtgaag tcggtagtg 
      aatacgtat cgactcaga atatttcaa tcgcgtcgg 
      agacggatt atgccagca tgaaaagaa gacagagca 
      aagcgactg gctagagaa ttgttttta ataaagttc 
      gtttcatga a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY