Status
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Gene information
General information
TC014251 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7977579..7983199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014251
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08272

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014251 RA
      MENIYRFKK RMVYPEKLT LDQVGDKTS SAKSSSWCN 
      GPLEKTHKI NVHSVKKTC KSEVREQNR SPSFENYST 
      SANRQFIQD RIRHLVDAF STRAQAARE RIETPATPS 
      SISSRETVE PPPVQIKPK LSTTSDKSI SEVPQTKSK 
      SNSIYRNIK YILDNKVID PNGKVYITW MSIAAMAVM 
      YNAWVIPLR STFPYQTPQ NRPVWMAFD YLADFVYLL 
      DMILIQPRV KYLNEGFWV TDMAQLRYN YIKKRAFKL 
      DLVSLIPLD LLYLILGPE CVILRFPRF FKLHTFWEF 
      FSFVDKLLA NPYIIRITR TLMYMMYLI HINACAYYA 
      FSSWEGIGS NNFVFDGVN NAYIKCFYF ATKTATSIG 
      KNPKPTQEI EYMFMTVSW LMGVFVFAL LIGQIRDII 
      STATRSKTE YRKLVDETL EYMRRLNLP QDMQRRVQL 
      WFNYTWETQ HTLDEKNIL DCLPHKMKT DIAINVHIQ 
      TLSKVKLFA DCDEALLRE LVLQLKSVI YLPGDIICK 
      KGDVGKEMY IIQCGKVQV IGRHENDVL ATLSEGSVF 
      GEISLLGIP GMNRRTADV RSQGHSNLF VLSKADLNA 
      ALSHYPEAQ ELLNKKAKM LMKKNAALE RKHNAMIII 
      DNPTPPAKQ AKLLDTVLQ VVPPDSKTN RLLRYGSKG 
      KPRGRPEND RFKYRNSLP SIKGIDRSF QTKVTVHRT 
      MS
    CDS sequence
    • >TC014251 RA
      atggaaaac atttaccgc ttcaaaaag agaatggtg 
      tatccggaa aagttaact ttggaccaa gtcggcgac 
      aagacatcc tctgcaaaa tcctcgtcc tggtgcaac 
      ggaccgttg gaaaaaacc cacaaaata aacgttcac 
      agtgttaaa aaaacgtgc aaaagtgaa gttcgcgag 
      cagaatagg tcgccttcc tttgaaaac tactccaca 
      tccgcaaat cggcaattt atccaggac cggatcagg 
      cacctggtc gatgctttt agcacacga gctcaagct 
      gcaagagaa cgaatcgaa actcctgcg acgccgtcc 
      tccatcagc agcagggaa acagtggag cctccgcca 
      gttcaaatc aagccaaaa ctgagcact acgtccgac 
      aagagcata agtgaagtt ccgcaaacc aagtccaag 
      tcgaattcc atttatagg aacattaag tacattttg 
      gataacaaa gttatcgac cccaatggc aaggtttac 
      atcacgtgg atgtcgatt gcagcgatg gctgttatg 
      tacaacgct tgggtcata cctctgagg agcactttt 
      ccgtatcaa acgcctcaa aataggcca gtgtggatg 
      gcgttcgat tacttagcc gattttgtt tacttgctc 
      gatatgatt ctcattcag ccaagagtc aaatatttg 
      aacgagggg ttttgggtt accgatatg gcgcagctc 
      agatacaac tacatcaag aaaagggct ttcaagttg 
      gacctggtc tcccttatc cctttggac ctcctctac 
      ttaatccta ggccccgaa tgcgtaatt ttgcgcttt 
      ccgcgcttc ttcaaattg cacacgttt tgggagttc 
      ttcagtttc gtggacaaa ctcctcgct aatccttac 
      ataatacgg ataacccgg accttgatg tacatgatg 
      tacctaatc cacataaac gcatgtgcg tattacgcc 
      ttctcctca tgggaaggc atcggaagc aataacttc 
      gtcttcgac ggtgtcaac aacgcctac attaaatgc 
      ttctacttc gccaccaaa actgcaacc tcgattgga 
      aaaaatcca aaaccgacc caagaaatc gagtatatg 
      ttcatgacg gtgtcatgg ctaatgggc gtgtttgta 
      ttcgctttg ttaataggg caaatacgc gacattatt 
      tcaacagcc acaaggtcg aagaccgaa tacagaaaa 
      ctagtagat gagactttg gagtacatg agaaggctg 
      aatttgccg caagatatg caaaggagg gtccaacta 
      tggttcaac tacacgtgg gagacgcaa cacacactt 
      gatgagaaa aacatcctc gactgtctt ccccacaag 
      atgaagacc gacattgcc ataaacgtc cacatccaa 
      accctcagc aaagtaaaa ctcttcgcc gactgtgac 
      gaagctctc cttcgcgag ctcgtcctc cagttgaaa 
      tcggtcatt tacctccct ggtgacata atttgcaaa 
      aagggcgac gttggtaaa gaaatgtac attatacag 
      tgcggcaaa gtccaagtt attggccgc cacgaaaac 
      gacgtgctt gcaactctc tccgaaggc tcggttttt 
      ggcgaaatc agtctcctg ggaatcccc ggaatgaac 
      cggcgaacc gccgacgtc cgctcgcaa ggacactca 
      aatctgttt gttctgagc aaagctgac ctgaacgcg 
      gccttgtcg cactacccc gaagctcaa gaattgctc 
      aacaagaag gcgaaaatg ctcatgaag aaaaacgcc 
      gctcttgaa agaaaacac aacgctatg attatcatc 
      gataatccc acgccgccg gcgaaacag gccaagttg 
      ctggatacg gtcctgcaa gtggtgccg cccgattcc 
      aaaactaac agacttttg aggtatggg agtaagggg 
      aagccgaga gggaggccg gagaacgat aggtttaag 
      tacaggaat agtttgcca agtatcaaa gggattgat 
      aggtcgttt cagactaaa gtgacagtg cacaggact 
      atgagttag
    mRNA sequence
    • >TC014251 RA
      cctagtagc aaactggat gacacgcaa cgcgcatta 
      atagtcaga aatggaaaa catttaccg cttcaaaaa 
      gagaatggt gtatccgga aaagttaac tttggacca 
      agtcggcga caagacatc ctctgcaaa atcctcgtc 
      ctggtgcaa cggaccgtt ggaaaaaac ccacaaaat 
      aaacgttca cagtgttaa aaaaacgtg caaaagtga 
      agttcgcga gcagaatag gtcgccttc ctttgaaaa 
      ctactccac atccgcaaa tcggcaatt tatccagga 
      ccggatcag gcacctggt cgatgcttt tagcacacg 
      agctcaagc tgcaagaga acgaatcga aactcctgc 
      gacgccgtc ctccatcag cagcaggga aacagtgga 
      gcctccgcc agttcaaat caagccaaa actgagcac 
      tacgtccga caagagcat aagtgaagt tccgcaaac 
      caagtccaa gtcgaattc catttatag gaacattaa 
      gtacatttt ggataacaa agttatcga ccccaatgg 
      caaggttta catcacgtg gatgtcgat tgcagcgat 
      ggctgttat gtacaacgc ttgggtcat acctctgag 
      gagcacttt tccgtatca aacgcctca aaataggcc 
      agtgtggat ggcgttcga ttacttagc cgattttgt 
      ttacttgct cgatatgat tctcattca gccaagagt 
      caaatattt gaacgaggg gttttgggt taccgatat 
      ggcgcagct cagatacaa ctacatcaa gaaaagggc 
      tttcaagtt ggacctggt ctcccttat ccctttgga 
      cctcctcta cttaatcct aggccccga atgcgtaat 
      tttgcgctt tccgcgctt cttcaaatt gcacacgtt 
      ttgggagtt cttcagttt cgtggacaa actcctcgc 
      taatcctta cataatacg gataacccg gaccttgat 
      gtacatgat gtacctaat ccacataaa cgcatgtgc 
      gtattacgc cttctcctc atgggaagg catcggaag 
      caataactt cgtcttcga cggtgtcaa caacgccta 
      cattaaatg cttctactt cgccaccaa aactgcaac 
      ctcgattgg aaaaaatcc aaaaccgac ccaagaaat 
      cgagtatat gttcatgac ggtgtcatg gctaatggg 
      cgtgtttgt attcgcttt gttaatagg gcaaatacg 
      cgacattat ttcaacagc cacaaggtc gaagaccga 
      atacagaaa actagtaga tgagacttt ggagtacat 
      gagaaggct gaatttgcc gcaagatat gcaaaggag 
      ggtccaact atggttcaa ctacacgtg ggagacgca 
      acacacact tgatgagaa aaacatcct cgactgtct 
      tccccacaa gatgaagac cgacattgc cataaacgt 
      ccacatcca aaccctcag caaagtaaa actcttcgc 
      cgactgtga cgaagctct ccttcgcga gctcgtcct 
      ccagttgaa atcggtcat ttacctccc tggtgacat 
      aatttgcaa aaagggcga cgttggtaa agaaatgta 
      cattataca gtgcggcaa agtccaagt tattggccg 
      ccacgaaaa cgacgtgct tgcaactct ctccgaagg 
      ctcggtttt tggcgaaat cagtctcct gggaatccc 
      cggaatgaa ccggcgaac cgccgacgt ccgctcgca 
      aggacactc aaatctgtt tgttctgag caaagctga 
      cctgaacgc ggccttgtc gcactaccc cgaagctca 
      agaattgct caacaagaa ggcgaaaat gctcatgaa 
      gaaaaacgc cgctcttga aagaaaaca caacgctat 
      gattatcat cgataatcc cacgccgcc ggcgaaaca 
      ggccaagtt gctggatac ggtcctgca agtggtgcc 
      gcccgattc caaaactaa cagactttt gaggtatgg 
      gagtaaggg gaagccgag agggaggcc ggagaacga 
      taggtttaa gtacaggaa tagtttgcc aagtatcaa 
      agggattga taggtcgtt tcagactaa agtgacagt 
      gcacaggac tatgagtta gtggaacta gttcaatta 
      ggggataat tttacagtc gattgaacg cgatatgat 
      ttacgagca atttatggt atttatgag gaagttggt 
      agtaggtca ggcctgcag gaacatgtc ttcaagttt 
      cttatctta caaattgga agataaata cataataat 
      ggagtgact cacttcatt aatataacg gtcaatgaa 
      aaatcactg tctattgct ttgaaaaaa tattgcgtc 
      atgtgaaaa tttagatga aactatgct gtgatggtt 
      ttttccaaa ataataaat tattttata aagtagcct 
      tctcgtaga tttggtgtg aactaaata gctataata 
      ttgtttgta gggaagtgt tacttgtat taaataata 
      ataataatt ttttaaatc atatcacat ttcattttc 
      tgaaagtaa taataagac tacgattag ttagctgtc 
      ttataattt ctagaattt ttttcccca gatagtaaa 
      tcctggctg gtctttgca tattcaaaa tttgcaaag 
      tgtaagtaa acagtttca atcaaaaat gccacaatt 
      taaatcgct gttaagata agaagtcag tggattatt 
      aaaaactct ctcctaaaa tagtctgtt aataggcaa 
      caggaaaaa actatgtcc ctatttatt gaaagaact 
      gttttaatt gtttatctt tttgaataa ttacctttt 
      gattgttta tctttttga ataattaca gttggtaat 
      cagaaaaag aattggtta aatgcgtag atctatttt 
      tacatactg ctgtaggaa acacctatt accaaaatt 
      ttgctgctt ggttttcat ttaacaaaa taaaaattt 
      gcgtcacaa aatattctt tgatgtttt ttttcatgt 
      atttgtcaa ttttttatt ttgttgttc attctatat 
      ctgcaaaaa atactttct tacaaatta aaatcatcc 
      gtgaaggtg gatttttca gtaatcctg attgtcatg 
      aaatcatct ttaaagtgt tcaaaacac tacacaaag 
      tgtgcaaat gtgtaatgt tggttctat aattgtcaa 
      agtttaatg tttacttca gcactaaag ttggtttat 
      ttttattac acttatatg gttttttct atattcgtg 
      ataaataat ttaacttta gtgccttca ttataacta 
      ttattttga atatatttt tctggcctt ccattcatt 
      acgaaataa ttgttgcgt ataaaaacc gagttattt 
      tggtggtaa aaatcatta gtataatcc ctggcacct 
      ttttatact ttacgacag gacttgtat tgcgaaggt 
      agaagcctt ggaagtgtg gcctttaat aaaaacaat 
      tattattgt tataataat tcaagctgt atttacatt 
      ttttatgct tttacgttt attgtagaa aataattat 
      tttttacgt attttgctg taattaggt cttacagaa 
      acattgtac cttacagta ttagctatt ttaggatac 
      tgattgtat aaaaagttg ctgtatttt tgtgataat 
      aaaaaaaag aaataaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY