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Gene information
General information
TC033103
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010398,TC010399
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7722641..7727728
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033103
    iBeetle screen iB_08268

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033103 RA
      MEWAYSGVN KTVPRNAGP ECADFMNPT WRLIDTVFV 
      LAFAVTLFK WSYSRITLP TVVYVRRDR RGRRALLVM 
      MSLIWGMEI GYKFSSRTV IYLLNPCHI TTAIQIYLL 
      AASPSKTVT AVFRVHLNL LNGPLLAFL FPETDTRIL 
      PLESAIYWI QHGMMFVVP YYLLRLGGV YNVEKFSDL 
      TWNAFSYSI NVMYHFAFL QTIAIPTEV NLNHMLCPA 
      ILDPFEGQL YRIFAVVHQ AILCPVLNK IFCLIANFF 
      LTKFWMTKV KSTLYYEVT DANGATDTG NHHHLH
    CDS sequence
    • >TC033103 RA
      atggagtgg gcctacagc ggagtcaac aaaaccgtg 
      ccccgaaat gcaggtccg gaatgcgct gattttatg 
      aaccccaca tggcgccta atcgacacc gtttttgtc 
      ctcgccttc gctgtgact ttgttcaag tggagctac 
      tccaggata acgctgccg acggtggtt tacgtccgg 
      agggatcgc agaggccgg cgggcgctc ctggtgatg 
      atgtcgctg atttgggga atggaaata ggctataag 
      ttctcatcg cgtactgtg atatatttg ttaaacccc 
      tgtcatata acaacagcg atccagata tatttgtta 
      gcggcttcc cccagcaaa acggtgaca gcggtgttc 
      cgagtgcat ttaaatctt ttaaatgga ccattattg 
      gcgtttttg ttcccagaa accgatact agaatattg 
      ccattagaa tctgcgata tattggatc cagcatgga 
      atgatgttt gtcgtgcct tattacctg cttagatta 
      ggaggggtt tacaatgtt gagaagttt tccgacctg 
      acttggaac gctttcagc tacagcatt aacgttatg 
      tatcatttc gcattttta caaacaatc gcaattcca 
      acggaagta aacctgaat cacatgtta tgtccggcc 
      atattggat cctttcgaa ggacaactg tatagaatt 
      tttgcagtg gtgcaccaa gcaatactc tgtccagta 
      ttgaacaaa atattctgc ttaattgca aatttcttt 
      ttaacgaag ttctggatg actaaagtc aaatcaacg 
      ttgtattac gaagttact gacgcaaat ggtgcaaca 
      gacactgga aaccaccac catctccac taa
    mRNA sequence
    • >TC033103 RA
      agctttggt gtcggcaat gcgtgcttt ctctaatca 
      atccaactg cattctgac gattactca tcacctgtg 
      aactacgtc aggatgtta ataaggtta gccctgacg 
      tgtggcttc tcaaaaaaa gccaccatt tcccgattt 
      tagcacagg tacaatgga ttgagcgaa aactaatta 
      caatggaaa ataaggtca aatgatgga gtgggccta 
      cagcggagt caacaaaac cgtgccccg aaatgcagg 
      tccggaatg cgctgattt tatgaaccc cacatggcg 
      cctaatcga caccgtttt tgtcctcgc cttcgctgt 
      gactttgtt caagtggag ctactccag gataacgct 
      gccgacggt ggtttacgt ccggaggga tcgcagagg 
      ccggcgggc gctcctggt gatgatgtc gctgatttg 
      gggaatgga aataggcta taagttctc atcgcgtac 
      tgtgatata tttgttaaa cccctgtca tataacaac 
      agcgatcca gatatattt gttagcggc ttcccccag 
      caaaacggt gacagcggt gttccgagt gcatttaaa 
      tcttttaaa tggaccatt attggcgtt tttgttccc 
      agaaaccga tactagaat attgccatt agaatctgc 
      gatatattg gatccagca tggaatgat gtttgtcgt 
      gccttatta cctgcttag attaggagg ggtttacaa 
      tgttgagaa gttttccga cctgacttg gaacgcttt 
      cagctacag cattaacgt tatgtatca tttcgcatt 
      tttacaaac aatcgcaat tccaacgga agtaaacct 
      gaatcacat gttatgtcc ggccatatt ggatccttt 
      cgaaggaca actgtatag aatttttgc agtggtgca 
      ccaagcaat actctgtcc agtattgaa caaaatatt 
      ctgcttaat tgcaaattt ctttttaac gaagttctg 
      gatgactaa agtcaaatc aacgttgta ttacgaagt 
      tactgacgc aaatggtgc aacagacac tggaaacca 
      ccaccatct ccactaaat tttaaatag aaataatta 
      cgtagaaat tacaacgta cctatacag ttgcaaata 
      aatatatct agcgtaagg aatacctaa tatcagtat 
      caactttct catattatt gcaaagaag ggtttttac 
      ttaccttag gaaactata aagtgggtg cttcataaa 
      tttcattcg gttctaatt aagagtgat ataaaagat 
      aagaatgta aatataaat cgacaaatg gcaaggcaa 
      aaattgtgt gcacgtaat tattttacg cattgtctt 
      ttataatta ttaataact tcggtataa ggtaattct 
      cacaaattt cattgaatc taaagtggt gtaaaaccc 
      gacaatagg cagcaagtg gccaaattc gtattctct 
      attatttaa aatattttt tccaattat cacttctgg 
      ttatttctt ttattagaa atgcgtatt gttaattta 
      tgtaatttt aattattta ataccattt gttgtcctt 
      tgtgtgaaa attctgcta aatttacat cgaacgcgc 
      tcgatgtga ttaaaggaa caacatagg ccactgatt 
      taataggaa gataaatat tgctcatag gtaaatcac 
      attcgttgg ggaattaac gaaagagct aacaaataa 
      atgtaaaaa taatctatt tcacgaaat cgtttcgtt 
      agattggct aacgaacgt gtaatgtaa tcttataat 
      aattaagta cctacttca gaacaaatt cacttagac 
      taagcatta accatatta aaattgctc tagtgtttg 
      ccacaaaac aatgatcgc gtccgatgt tttgtaatt 
      tttttaatg aaactaagg ccatacata agtgcacga 
      ccggattgt aaagcgaat aggaaaatg aatgaattc 
      tgagcaaga aacagtcga gtgcccctc aactatcat 
      atcatttgt tgaaaacct agattgttt tataatgtg 
      atttaggca aagtacaat tcttttttg catttattc 
      tcatattcg ctgtacacg acacatact tgaaacttg 
      tgatctgta aagtgtgta aaattatgt taatttgcg 
      acaatatat tgttacaat tatagcatt acgagcttt 
      ttatagaaa aatatcgat agttttgca ggttgttct 
      caatactag tttcggacg actttctac atcaaatat 
      gaattaagt aaacgaatt ccattactt gaacacgtg 
      tatacttac tttgcaaac ttccagatt agtttccta 
      ttgttacaa gaaagttac taaataata attcacaat 
      aaaatatat cgttacgta cccagtttt tgtattttt 
      aatttcttt cactaccta ctggttgaa ataaaacat 
      gcaagcaac attttattg ttatattta aaaacaatt 
      tatacaaac cataaatac ctactatac ttgcacata 
      ccttgtaat aaaaatggt aactattgt aaatgaaat 
      tgacttggt aaaaaatgc acaaccaaa taaactaac 
      agatgcaag gcttcggtt gatcgcacg agcgcaatt 
      gatttgtct cgtgtgaac aactcgtta tttgtacga 
      aagcgtatc ccgccattt cctaaaata cgaaataaa 
      taacaaagt gctccctaa catcgttaa attaagtac 
      gcgattttt aaagtccta atattaaaa atcaagaaa 
      ttatttaca tttgcgcgc acctgcaca cgatttcgt 
      ggaaggaac agtccttga ataatgcta aaaacgtgc 
      ataatgcaa gtaaaaaat ccttttgtt atatttcta 
      gtataaata atgcagaca ttctgatac aattgtgtc 
      tatttataa aaatattat gcaggatta atgcgtttt 
      ctgtttttg cactgcacg cattgactt ccaattgga 
      gttcgattt aaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY