Status
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Gene information
General information
TC010431 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7321376..7327453
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010431
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08260

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010431 RA
      MNGQMKSSP ESQLQMAIS ILLESIADK SQIVIETVN 
      SSIRKLAEK HPNQVLNSC CHFCERTPK PTNEHLSAI 
      LSIMEKICI EHIVAIDGD TILLMIDFS LRTMIENQN 
      YEPIVQMPA SNVLVALGR KHHIQVMES LLGKLEVGV 
      IPHYMIPHT LGALANANA FGVIPYLKN ILAIMLPLL 
      GGVKFDPLK QAFSYALGS FCEALVEYT SNIDQVPDN 
      TITIENFQV EIGMAYDIL FSSWLQTRE PKVAASVLN 
      ALSAIFLVL SVEKVTQHT PRIIHTLLN LYKRQVDAY 
      NITKCLGAI IQKAARVNG TLLEPLLSN ILQVLSDLV 
      CVSPDYAQP DLLRNHSEV LRCYECFAL HFTDNTVDQ 
      LIMQLKNNN EKERIKAIL VITHLTSYS TEHAIQRRF 
      KDIVRHLSE MLNDPNIRV KKAMMKIIV AFACKGVLL 
      NKEINPDGP EKYMEFLLK LCCKPANLK NNEIDGGEL 
      FDIQKSADN TLCMLSTSV PELESTLWS LLMQCFLGP 
      SYDDAVVVL LRCLTHLAS RKTTTEVCE GAFVRCVTL 
      LAMPLPGFR GTFLLNFLK NIRPCDVER YKSVWDLKI 
      PQLVKYLEQ NYDNFNHLE WQDLVFDFL SILLESIKD 
      QKFNETLIL TARKQLEMY NNNRYMSSG ADPSVKQTE 
      KHFLLKCLA MILCHSQEK EIILQTLDD ILVNAKLTD 
      YSELNACAE AVGICSRVH LQYVLDKLS LIRKDVLLK 
      KSSKFFSFL KDQKHELGI ERIRYVIVS SYAEICNEA 
      STDKLLKVI ESEILDFVV NELTNAKDF SIRKVCLRA 
      IGSVGDAMH PNRNSLHIR MLDRDKVLH LVASQVHLH 
      SGPEYIELF PVILPVITS LVRLPLPLE SDQRLKLLK 
      LCFDNVYNA SAIYCKINS DNDNYYGDL KLVPFVTSS 
      FTKLNQLVQ ELLMQTLSP ATLDEIVTL LEPWLRRRK 
      AEQRLPAVE TLRLVLQTY LDNMKFAYD CPTTFGQTG 
      SLLAKIVPR CTDPNKNIR KVATECLCL VLCIASRYE 
      GHMRDHDKQ LSNSMQHIQ QQIDSDDPK LLYNLTSDL 
      AHIICVNLP HFQLIHLVD GLIDALLDC EASSSNGSS 
      VVLNMTLKS KGSELQGHV NTILDKLII QLGKIQCAR 
      TRSSTLRSI LNFATHHSK AVCQILLNQ PLPFDGSIC 
      DCWAVLSTD QTLVLDLFD NLKKVIKQT ALYEEQGNG 
      EIKIATLQP LQAVCAFHE LLKNTQLKD ICKQHFPEI 
      FAIFLVTLA SYIGTSAPA IKFNSDKKE KYGFVLNRE 
      AFKLKPAKI ALETLKSFL ICCAYTQTA GCLTNLINV 
      EANEELSAF LETMSCLVE DVCVENPES LSWLVACLG 
      PYIRAELEP QRVAVVAFF AYLLKQKAN HQTVLAENL 
      LEMVLDVQM DSSCLVRKI ALQALGFAA EALNTDLVS 
      RHCSPILSV LMNSLDYNS IGNESDVIL EGMLSFSKL 
      LTTLEGRKF GTCQVTAAV RIKPLLDQE DVSLRRASF 
      RLLGDLAAS LGPEANVEA FKEQVQGNF ITLILHLCD 
      PDVNVIKTC KYTLRKVAI YLDSPKVNS MVQEHLIDE 
      ANLHYVDFI KDLVKLMAE EMQDLFNLF VMTSLSYLK 
      SPWVPIRSS AALMVGLLF SQLSAENKQ RISVDTVCD 
      KLMRLVNDE HEEVRMRAV QAIAYLFID
    CDS sequence
    • >TC010431 RA
      atgaatggt caaatgaaa agtagccca gaatcgcaa 
      cttcagatg gctattagc atccttctg gagtcaata 
      gctgataag agccagatt gtgattgag acggtcaat 
      agttcaata cgaaaatta gccgagaag cacccaaat 
      caagtcctc aactcttgc tgtcacttc tgcgaacgt 
      accccaaaa ccaacaaac gaacacctc tctgcgata 
      ctctcaata atggaaaaa atatgcatt gaacacatt 
      gttgcaatc gatggtgac acaattctc ctaatgatt 
      gacttttct ttgcgtact atgatcgaa aatcaaaat 
      tacgagccc attgtccaa atgccagct tcaaacgtt 
      ttagtggct ttggggcga aaacaccac attcaggtg 
      atggaaagc cttctcgga aaattagaa gttggggtc 
      ataccgcac tacatgatc ccacacact ttaggagcc 
      ttagcaaac gccaacgct tttggtgtt atcccctat 
      ctcaaaaat attcttgcc attatgttg ccactttta 
      ggcggagtt aaattcgat ccgttgaaa caagctttt 
      tcttacgct ttgggtagt ttttgcgag gctttggtt 
      gaatacacc tcaaacata gatcaagtg cctgataac 
      actatcact atagaaaac ttccaagtc gaaataggc 
      atggcctac gacatttta ttctcttcg tggttgcaa 
      acacgcgaa cccaaagtc gcagcaagt gtccttaac 
      gctctatct gctattttc ctcgtatta agtgtggag 
      aaagtcact caacacact cccagaatc atccacact 
      ctcttgaac ttgtacaag aggcaagta gatgcttac 
      aacattact aaatgtttg ggggcaata atacaaaag 
      gctgcacgc gtcaatgga acgttactg gagcctctc 
      ctttcaaac attttgcaa gttttatcc gacttagtg 
      tgcgtttct cccgattac gctcaacca gacctcttg 
      agaaatcat tcagaggta ttgaggtgt tacgagtgt 
      ttcgctctc cattttacc gacaatacc gtcgaccag 
      cttatcatg caactgaaa aacaataac gaaaaagag 
      cgaattaaa gcgatttta gtcatcaca catttgaca 
      tcgtattca accgaacac gcgattcag aggaggttc 
      aaagacatt gtccgccac ttgagcgaa atgctcaac 
      gatccgaac ataagggtg aagaaagcc atgatgaaa 
      ataatcgtg gcgtttgct tgtaaaggg gttctttta 
      aataaagaa atcaacccc gacggtccc gaaaaatac 
      atggaattt cttctaaaa ttgtgctgc aagccggcc 
      aatttaaaa aataacgaa atagacgga ggcgaactc 
      tttgacata caaaaatca gccgacaac actttatgc 
      atgttgagt acttctgtt cccgagctg gagtcaact 
      ttgtggagt cttttgatg cagtgtttc ctcggtccg 
      tcttacgat gacgctgtt gttgttcta ttgcgttgt 
      ttgactcac ttagcatcg aggaaaaca acaacagaa 
      gtgtgcgaa ggcgctttt gtccgttgt gtgacttta 
      ctagccatg cctttgccc ggttttaga ggcactttt 
      ttgttgaat tttctcaaa aatatccga ccttgtgac 
      gtagagcgg tacaaatcg gtctgggat ttgaaaatc 
      ccgcaacta gtcaagtat ttagagcag aattatgat 
      aattttaac cacttggaa tggcaggac ttggtgttt 
      gattttctg agtattttg cttgagtct ataaaagat 
      cagaagttc aacgagacg cttatttta acagcgcgg 
      aaacaacta gaaatgtac aataataac agatacatg 
      agcagcggg gccgaccct tcagtcaaa caaaccgag 
      aaacacttt ctgctcaaa tgcttggca atgatcctg 
      tgccactcg caagagaaa gaaattatt ctacaaact 
      ttggacgat attttagta aatgcgaag ctgactgat 
      tactctgaa ctcaacgct tgtgctgaa gctgtcggt 
      atttgttca agagtccac ttgcagtac gtcttggac 
      aaactctcc ctgatcaga aaagacgtc ttacttaaa 
      aaatcgtcg aagtttttt tctttcctc aaagaccag 
      aaacacgaa ttggggatt gagaggata aggtatgtt 
      atagtctcg agttacgct gagatttgt aacgaggct 
      tccaccgat aagttactg aaagtgatc gagtctgaa 
      atcctcgat ttcgttgta aatgaatta acaaatgct 
      aaggatttt tccattcgt aaagtttgc ttacgtgca 
      attggaagt gtgggcgat gccatgcat ccaaacaga 
      aattcgctc catatccga atgcttgac agggacaag 
      gttttgcat ttggtggct tcacaggtg cacctgcac 
      agcggtccc gaatacatc gagttgttc ccggtgatt 
      ttgccagtg attacgtct ctagtgcgc ctcccgttg 
      ccactcgag tcggaccaa aggctgaaa ttactgaaa 
      ctttgcttc gataatgtg tacaatgct tccgcaatt 
      tattgtaaa ataaacagt gataacgat aattactac 
      ggggacttg aaattagtc ccgttcgtc acatcaagc 
      ttcacgaag ttgaatcag ctcgtccag gagttgcta 
      atgcagact ttgtcacct gcgacgctg gatgagatc 
      gtgacgttg cttgaacct tggctgagg cgaagaaag 
      gcggaacaa aggcttcca gcggttgag actttaaga 
      ctggtcctt cagacgtac ttagacaat atgaaattt 
      gcgtacgat tgcccgaca acgtttggg cagacgggg 
      tctctgctg gcgaagata gtgcccagg tgcaccgac 
      cccaacaaa aatattaga aaggtggca actgagtgt 
      ctctgtctt gtcctttgc atcgcttcg cgatacgag 
      ggtcacatg cgggaccac gacaaacag ctaagcaat 
      tcaatgcaa cacatccag caacaaatc gactcagac 
      gatcccaaa ctcctgtac aatctaaca tcagacttg 
      gcccacata atatgcgta aacctcccc cacttccaa 
      ctcatccac cttgtggat ggcctaatt gacgctttg 
      ctggactgc gaagcctca agttcgaat ggcagcagc 
      gtggtgcta aacatgaca ctcaaaagc aagggtagc 
      gagctccaa ggccacgtc aacacgatt ctggacaaa 
      ctaataatc caacttggc aaaattcaa tgtgccaga 
      acccgttcg tcaacatta cggagcatt ttaaatttt 
      gcaacccac cattcgaaa gccgtgtgc caaatcctg 
      ctcaaccaa cccttgccg ttcgacggt tcaatttgc 
      gattgttgg gctgtttta tcaaccgac caaacccta 
      gttttggat ttattcgac aatttgaag aaagtcatc 
      aaacaaaca gcgctttat gaagagcaa ggcaacggc 
      gagattaaa attgcgacc ttgcagccg ttgcaagcc 
      gtgtgcgcc tttcacgag ttgctgaaa aacacccaa 
      ttgaaggac atctgcaag cagcacttc cccgaaata 
      ttcgcgatt tttttggtg actttggct tcgtatatt 
      ggaacctct gccccagct atcaaattc aacagcgat 
      aaaaaggag aaatacggt tttgttcta aaccgggaa 
      gctttcaag ctaaaaccc gccaaaatc gcgttagaa 
      acgttgaaa tcgtttctg atttgctgc gcctacacg 
      cagactgcg ggttgtttg accaattta attaacgtg 
      gaggcgaat gaagagttg tcggcgttc ctggaaacc 
      atgtcgtgt ctcgttgaa gacgtgtgt gtggagaat 
      cccgagtcg ctttcgtgg ctggtggcc tgcttgggg 
      ccgtatatt cgggccgag ttggagcca caaagagtg 
      gccgttgtg gcgttcttt gcctatttg ttgaaacag 
      aaagcgaat catcaaact gttcttgcg gagaatttg 
      ctagaaatg gtcttggat gtgcagatg gattccagc 
      tgcttggtt aggaagatt gctctgcag gcgttgggg 
      tttgcagca gaggctctg aacacggat ttggtctcc 
      aggcattgt tccccgatt ttgagcgtg ctgatgaat 
      agtctggat tacaacagc atcggaaac gaaagcgac 
      gtcatcctt gagggaatg ctgagtttt tcgaaactg 
      ttgacaacc ctcgagggt cgcaaattc ggcacgtgt 
      caagtgacg gcagctgtg cgaatcaag ccgctcctt 
      gaccaggaa gacgtctca cttcgcagg gcttctttt 
      cgcctcttg ggcgatttg gcggcctcc ttgggcccc 
      gaagccaac gttgaagcg ttcaaagaa caggttcaa 
      ggcaacttc attaccctt attctgcac ttgtgcgat 
      cccgacgtt aacgtgatc aaaacttgc aagtacact 
      ttacgcaaa gtggcgatt tatttggac agccccaaa 
      gtgaactcg atggttcag gagcatcta attgacgaa 
      gcgaacttg cattacgtc gatttcatc aaagatttg 
      gttaaatta atggcggag gaaatgcaa gatttgttc 
      aatttgttc gtgatgacg agtttgtcg tacttgaag 
      agcccgtgg gttccgatc aggagcagc gcggctctt 
      atggtgggg ctgctattt tcacaattg agtgctgag 
      aataagcaa aggatctcg gtggatacg gtttgtgat 
      aagttgatg agattggtt aatgacgag cacgaggag 
      gtgaggatg agggccgtg caagcgatc gcgtactta 
      tttattgat taa
    mRNA sequence
    • >TC010431 RA
      agccaaaaa ttatcagac aaataacaa actaataat 
      taaccaaaa tgaatggtc aaatgaaaa gtagcccag 
      aatcgcaac ttcagatgg ctattagca tccttctgg 
      agtcaatag ctgataaga gccagattg tgattgaga 
      cggtcaata gttcaatac gaaaattag ccgagaagc 
      acccaaatc aagtcctca actcttgct gtcacttct 
      gcgaacgta ccccaaaac caacaaacg aacacctct 
      ctgcgatac tctcaataa tggaaaaaa tatgcattg 
      aacacattg ttgcaatcg atggtgaca caattctcc 
      taatgattg acttttctt tgcgtacta tgatcgaaa 
      atcaaaatt acgagccca ttgtccaaa tgccagctt 
      caaacgttt tagtggctt tggggcgaa aacaccaca 
      ttcaggtga tggaaagcc ttctcggaa aattagaag 
      ttggggtca taccgcact acatgatcc cacacactt 
      taggagcct tagcaaacg ccaacgctt ttggtgtta 
      tcccctatc tcaaaaata ttcttgcca ttatgttgc 
      cacttttag gcggagtta aattcgatc cgttgaaac 
      aagcttttt cttacgctt tgggtagtt tttgcgagg 
      ctttggttg aatacacct caaacatag atcaagtgc 
      ctgataaca ctatcacta tagaaaact tccaagtcg 
      aaataggca tggcctacg acattttat tctcttcgt 
      ggttgcaaa cacgcgaac ccaaagtcg cagcaagtg 
      tccttaacg ctctatctg ctattttcc tcgtattaa 
      gtgtggaga aagtcactc aacacactc ccagaatca 
      tccacactc tcttgaact tgtacaaga ggcaagtag 
      atgcttaca acattacta aatgtttgg gggcaataa 
      tacaaaagg ctgcacgcg tcaatggaa cgttactgg 
      agcctctcc tttcaaaca ttttgcaag ttttatccg 
      acttagtgt gcgtttctc ccgattacg ctcaaccag 
      acctcttga gaaatcatt cagaggtat tgaggtgtt 
      acgagtgtt tcgctctcc attttaccg acaataccg 
      tcgaccagc ttatcatgc aactgaaaa acaataacg 
      aaaaagagc gaattaaag cgattttag tcatcacac 
      atttgacat cgtattcaa ccgaacacg cgattcaga 
      ggaggttca aagacattg tccgccact tgagcgaaa 
      tgctcaacg atccgaaca taagggtga agaaagcca 
      tgatgaaaa taatcgtgg cgtttgctt gtaaagggg 
      ttcttttaa ataaagaaa tcaaccccg acggtcccg 
      aaaaataca tggaatttc ttctaaaat tgtgctgca 
      agccggcca atttaaaaa ataacgaaa tagacggag 
      gcgaactct ttgacatac aaaaatcag ccgacaaca 
      ctttatgca tgttgagta cttctgttc ccgagctgg 
      agtcaactt tgtggagtc ttttgatgc agtgtttcc 
      tcggtccgt cttacgatg acgctgttg ttgttctat 
      tgcgttgtt tgactcact tagcatcga ggaaaacaa 
      caacagaag tgtgcgaag gcgcttttg tccgttgtg 
      tgactttac tagccatgc ctttgcccg gttttagag 
      gcacttttt tgttgaatt ttctcaaaa atatccgac 
      cttgtgacg tagagcggt acaaatcgg tctgggatt 
      tgaaaatcc cgcaactag tcaagtatt tagagcaga 
      attatgata attttaacc acttggaat ggcaggact 
      tggtgtttg attttctga gtattttgc ttgagtcta 
      taaaagatc agaagttca acgagacgc ttattttaa 
      cagcgcgga aacaactag aaatgtaca ataataaca 
      gatacatga gcagcgggg ccgaccctt cagtcaaac 
      aaaccgaga aacactttc tgctcaaat gcttggcaa 
      tgatcctgt gccactcgc aagagaaag aaattattc 
      tacaaactt tggacgata ttttagtaa atgcgaagc 
      tgactgatt actctgaac tcaacgctt gtgctgaag 
      ctgtcggta tttgttcaa gagtccact tgcagtacg 
      tcttggaca aactctccc tgatcagaa aagacgtct 
      tacttaaaa aatcgtcga agttttttt ctttcctca 
      aagaccaga aacacgaat tggggattg agaggataa 
      ggtatgtta tagtctcga gttacgctg agatttgta 
      acgaggctt ccaccgata agttactga aagtgatcg 
      agtctgaaa tcctcgatt tcgttgtaa atgaattaa 
      caaatgcta aggattttt ccattcgta aagtttgct 
      tacgtgcaa ttggaagtg tgggcgatg ccatgcatc 
      caaacagaa attcgctcc atatccgaa tgcttgaca 
      gggacaagg ttttgcatt tggtggctt cacaggtgc 
      acctgcaca gcggtcccg aatacatcg agttgttcc 
      cggtgattt tgccagtga ttacgtctc tagtgcgcc 
      tcccgttgc cactcgagt cggaccaaa ggctgaaat 
      tactgaaac tttgcttcg ataatgtgt acaatgctt 
      ccgcaattt attgtaaaa taaacagtg ataacgata 
      attactacg gggacttga aattagtcc cgttcgtca 
      catcaagct tcacgaagt tgaatcagc tcgtccagg 
      agttgctaa tgcagactt tgtcacctg cgacgctgg 
      atgagatcg tgacgttgc ttgaacctt ggctgaggc 
      gaagaaagg cggaacaaa ggcttccag cggttgaga 
      ctttaagac tggtccttc agacgtact tagacaata 
      tgaaatttg cgtacgatt gcccgacaa cgtttgggc 
      agacggggt ctctgctgg cgaagatag tgcccaggt 
      gcaccgacc ccaacaaaa atattagaa aggtggcaa 
      ctgagtgtc tctgtcttg tcctttgca tcgcttcgc 
      gatacgagg gtcacatgc gggaccacg acaaacagc 
      taagcaatt caatgcaac acatccagc aacaaatcg 
      actcagacg atcccaaac tcctgtaca atctaacat 
      cagacttgg cccacataa tatgcgtaa acctccccc 
      acttccaac tcatccacc ttgtggatg gcctaattg 
      acgctttgc tggactgcg aagcctcaa gttcgaatg 
      gcagcagcg tggtgctaa acatgacac tcaaaagca 
      agggtagcg agctccaag gccacgtca acacgattc 
      tggacaaac taataatcc aacttggca aaattcaat 
      gtgccagaa cccgttcgt caacattac ggagcattt 
      taaattttg caacccacc attcgaaag ccgtgtgcc 
      aaatcctgc tcaaccaac ccttgccgt tcgacggtt 
      caatttgcg attgttggg ctgttttat caaccgacc 
      aaaccctag ttttggatt tattcgaca atttgaaga 
      aagtcatca aacaaacag cgctttatg aagagcaag 
      gcaacggcg agattaaaa ttgcgacct tgcagccgt 
      tgcaagccg tgtgcgcct ttcacgagt tgctgaaaa 
      acacccaat tgaaggaca tctgcaagc agcacttcc 
      ccgaaatat tcgcgattt ttttggtga ctttggctt 
      cgtatattg gaacctctg ccccagcta tcaaattca 
      acagcgata aaaaggaga aatacggtt ttgttctaa 
      accgggaag ctttcaagc taaaacccg ccaaaatcg 
      cgttagaaa cgttgaaat cgtttctga tttgctgcg 
      cctacacgc agactgcgg gttgtttga ccaatttaa 
      ttaacgtgg aggcgaatg aagagttgt cggcgttcc 
      tggaaacca tgtcgtgtc tcgttgaag acgtgtgtg 
      tggagaatc ccgagtcgc tttcgtggc tggtggcct 
      gcttggggc cgtatattc gggccgagt tggagccac 
      aaagagtgg ccgttgtgg cgttctttg cctatttgt 
      tgaaacaga aagcgaatc atcaaactg ttcttgcgg 
      agaatttgc tagaaatgg tcttggatg tgcagatgg 
      attccagct gcttggtta ggaagattg ctctgcagg 
      cgttggggt ttgcagcag aggctctga acacggatt 
      tggtctcca ggcattgtt ccccgattt tgagcgtgc 
      tgatgaata gtctggatt acaacagca tcggaaacg 
      aaagcgacg tcatccttg agggaatgc tgagttttt 
      cgaaactgt tgacaaccc tcgagggtc gcaaattcg 
      gcacgtgtc aagtgacgg cagctgtgc gaatcaagc 
      cgctccttg accaggaag acgtctcac ttcgcaggg 
      cttcttttc gcctcttgg gcgatttgg cggcctcct 
      tgggccccg aagccaacg ttgaagcgt tcaaagaac 
      aggttcaag gcaacttca ttaccctta ttctgcact 
      tgtgcgatc ccgacgtta acgtgatca aaacttgca 
      agtacactt tacgcaaag tggcgattt atttggaca 
      gccccaaag tgaactcga tggttcagg agcatctaa 
      ttgacgaag cgaacttgc attacgtcg atttcatca 
      aagatttgg ttaaattaa tggcggagg aaatgcaag 
      atttgttca atttgttcg tgatgacga gtttgtcgt 
      acttgaaga gcccgtggg ttccgatca ggagcagcg 
      cggctctta tggtggggc tgctatttt cacaattga 
      gtgctgaga ataagcaaa ggatctcgg tggatacgg 
      tttgtgata agttgatga gattggtta atgacgagc 
      acgaggagg tgaggatga gggccgtgc aagcgatcg 
      cgtacttat ttattgatt aaaattctc ccctttttg 
      gtatttatt cattccatg ctattatgg gttgcaatc 
      gcagagctg atggacgat ttacgtaaa ttgtataaa 
      aagataaat gcaccttac ctgagtcct tttcaattt 
      gcaattttt ggcgcttat ttgaatgag aatatgcca 
      caaaattaa actgaaaat attgacatt tatgtcaaa 
      taggtcact gtgcatagc aatcgtgat tttctagtt 
      tttgtataa gat
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