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Gene information
General information
TC014151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6771310..6775053
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014151
    iBeetle screen iB_08254

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014151 RA
      MEKSKKYIT ISVAAFICY CNTLWGSFV FDDTEAIVK 
      NKDVMPYIP LNEIFRNDF WGSNISLNS SHKSYRPLT 
      ILSYRLNVL YSNNKLDAF QFHATNVIL YGLLCLLTI 
      PVFELFLRK KKTQKSVDD TAYLSSLLF TVHPIHTEC 
      VAGLVGRAD ILCSILFFI VILLYDKAI SKKSFVLLC 
      IVVVTTAAA VLCKETAIT VLGVCVVYD LFLLKKQRK 
      DWCDIFNIY FFIRTATLV VTGLVILFY RFKIMNFEG 
      PTFTSIDNP AAYADKVFI RIFSYNYIY FLNFLLLLW 
      PQWLCFDWS MGCIPLIER ISDKRILIV LIFWIITLI 
      SVHHLAKKV VENRFLDAK TMALGLIIF PFLPASNLF 
      LKVGFVIAE RTLLLPSAG FCLLVVIGL KKIQSFAPS 
      HKTTVVVLF YTVCVIFTL RSIQRNFEW LREETLFTS 
      ALNVCPLNA KVHYNIAKI AGDQNNKTL ALSEYKKAL 
      ELNPNYEQA MNNLANLLR EDRKFGEAE ALLRKALDV 
      RPNFAAAWM NLGIVLTNL NRTEEAEHC YKTAIKFRN 
      KYPDCYYNL GNLYLDQKR NDEALDAWE MAVLYRPSH 
      VAAWSNTLV LLDSMRQYD KVLELGITA LIHNPKSPA 
      LHFSLANTL GKIQRFEKA ESHFLEAIN LNPNNALYH 
      SNLGVLYHR WGKVDKARE MYKKALQID PHMKTTELN 
      LRKLVNAQD
    CDS sequence
    • >TC014151 RA
      atggaaaaa tctaagaaa tacatcaca atttccgta 
      gcggcgttt atttgttac tgcaataca ctctggggc 
      tcattcgtc ttcgacgac accgaagca atagtcaaa 
      aacaaagat gtgatgcct tacattcca ctaaacgag 
      atattccga aacgacttt tggggcagc aatatctcg 
      cttaacagt agtcacaag tcctataga cctctcact 
      attctgtct tatagactg aacgtttta tattccaat 
      aataaacta gacgcattc caatttcac gcaaccaat 
      gtgattctt tacggactg ttatgttta ctcaccatt 
      cccgtgttt gaattgttt ctccgcaaa aagaaaact 
      caaaaatcc gttgacgac actgcctat ttatcaagc 
      ttgttattc acagtccac cccattcac acagagtgt 
      gtcgccgga ctcgtcggc agggccgat attttgtgc 
      tccatttta ttcttcatt gtgatatta ttgtatgat 
      aaagcaatt agcaaaaaa tcgtttgtg ttactttgt 
      atcgttgtg gtgaccaca gcggctgct gtcttatgt 
      aaagaaacg gcgataact gttttgggc gtttgtgtt 
      gtttatgat ctttttttg ttgaaaaaa caaaggaaa 
      gattggtgc gacatattt aatatttat ttttttata 
      cgtactgcg actctggtt gtgacagga ttagtcatt 
      ttgttttat aggtttaaa attatgaac ttcgaaggc 
      cctactttt acgagcatc gataaccca gctgcgtat 
      gccgacaaa gtttttatt agaatattt tcctacaac 
      tatatttat tttttgaat tttttgctc ttgttgtgg 
      ccacagtgg ctgtgtttt gactggtct atggggtgc 
      atacctttg attgaaaga atctcggat aaaagaata 
      ctgatcgtc ttgattttc tggataatt accctcata 
      tctgtgcat catttggct aaaaaagtc gtcgaaaac 
      agattcttg gacgctaaa accatggca cttggttta 
      ataattttc ccattttta ccagcttca aatttattc 
      ctgaaagtg gggtttgtg atagctgaa cgtactcta 
      ctattacct agcgctggg ttttgtcta ctggttgtg 
      atcggttta aaaaaaatc cagtctttt gctcctagt 
      cacaaaacg actgtagtt gtgttattt tacacagtt 
      tgtgtcatt tttaccctt cgctcaatt caacgaaac 
      ttcgaatgg ctgcgagag gaaacactg ttcacttca 
      gctctcaat gtgtgtcct ctcaatgcc aaagtgcac 
      tacaacatc gccaaaata gccggcgac caaaacaac 
      aaaactttg gctttatcc gagtataaa aaagccctc 
      gagctcaac ccgaactac gaacaggcg atgaacaac 
      ttggccaac ttattgcgt gaagaccgg aagtttggc 
      gaagccgaa gccttgctc cgcaaagca cttgatgtt 
      aggccaaat ttcgcggcc gcttggatg aatctgggg 
      atagttttg acgaattta aatcggacc gaggaggcc 
      gagcattgc tacaaaacc gcgatcaag ttcaggaat 
      aaatacccg gattgttat tacaacttg ggcaatttg 
      tatttggac caaaagcgc aacgacgag gctctggac 
      gcgtgggaa atggccgta ctttaccgc ccttcgcac 
      gtggcggct tggagcaac accttggtg ctcctggac 
      agtatgaga caatacgac aaagtgcta gagttggga 
      attaccgcc cttatccac aacccgaaa tccccggcc 
      ctgcatttc agtcttgcc aacacgctg gggaaaatc 
      cagcggttc gaaaaagcc gaaagtcat tttctggaa 
      gcgatcaat ttgaatcca aacaacgct ctctaccac 
      tcgaattta ggggtttta tatcacaga tggggtaaa 
      gttgataag gctcgtgag atgtataaa aaggcactt 
      cagattgat ccccatatg aaaacgacc gaactgaat 
      cttcgcaaa ttggtgaat gctcaagat tga
    mRNA sequence
    • >TC014151 RA
      gcggctgta aatttcaaa cgtggggat tttcagtaa 
      cgctttcac gcttctcgc attacaaaa cactttgtg 
      aggttaaaa aatttgaca accatcaaa caaatgatg 
      aaataaaac aaaatttta aatttctcc aatttatta 
      aatgaagcc cgactaaaa tggaaaaat ctaagaaat 
      acatcacaa tttccgtag cggcgttta tttgttact 
      gcaatacac tctggggct cattcgtct tcgacgaca 
      ccgaagcaa tagtcaaaa acaaagatg tgatgcctt 
      acattccac taaacgaga tattccgaa acgactttt 
      ggggcagca atatctcgc ttaacagta gtcacaagt 
      cctatagac ctctcacta ttctgtctt atagactga 
      acgttttat attccaata ataaactag acgcattcc 
      aatttcacg caaccaatg tgattcttt acggactgt 
      tatgtttac tcaccattc ccgtgtttg aattgtttc 
      tccgcaaaa agaaaactc aaaaatccg ttgacgaca 
      ctgcctatt tatcaagct tgttattca cagtccacc 
      ccattcaca cagagtgtg tcgccggac tcgtcggca 
      gggccgata ttttgtgct ccattttat tcttcattg 
      tgatattat tgtatgata aagcaatta gcaaaaaat 
      cgtttgtgt tactttgta tcgttgtgg tgaccacag 
      cggctgctg tcttatgta aagaaacgg cgataactg 
      ttttgggcg tttgtgttg tttatgatc tttttttgt 
      tgaaaaaac aaaggaaag attggtgcg acatattta 
      atatttatt tttttatac gtactgcga ctctggttg 
      tgacaggat tagtcattt tgttttata ggtttaaaa 
      ttatgaact tcgaaggcc ctactttta cgagcatcg 
      ataacccag ctgcgtatg ccgacaaag tttttatta 
      gaatatttt cctacaact atatttatt ttttgaatt 
      ttttgctct tgttgtggc cacagtggc tgtgttttg 
      actggtcta tggggtgca tacctttga ttgaaagaa 
      tctcggata aaagaatac tgatcgtct tgattttct 
      ggataatta ccctcatat ctgtgcatc atttggcta 
      aaaaagtcg tcgaaaaca gattcttgg acgctaaaa 
      ccatggcac ttggtttaa taattttcc catttttac 
      cagcttcaa atttattcc tgaaagtgg ggtttgtga 
      tagctgaac gtactctac tattaccta gcgctgggt 
      tttgtctac tggttgtga tcggtttaa aaaaaatcc 
      agtcttttg ctcctagtc acaaaacga ctgtagttg 
      tgttatttt acacagttt gtgtcattt ttacccttc 
      gctcaattc aacgaaact tcgaatggc tgcgagagg 
      aaacactgt tcacttcag ctctcaatg tgtgtcctc 
      tcaatgcca aagtgcact acaacatcg ccaaaatag 
      ccggcgacc aaaacaaca aaactttgg ctttatccg 
      agtataaaa aagccctcg agctcaacc cgaactacg 
      aacaggcga tgaacaact tggccaact tattgcgtg 
      aagaccgga agtttggcg aagccgaag ccttgctcc 
      gcaaagcac ttgatgtta ggccaaatt tcgcggccg 
      cttggatga atctgggga tagttttga cgaatttaa 
      atcggaccg aggaggccg agcattgct acaaaaccg 
      cgatcaagt tcaggaata aatacccgg attgttatt 
      acaacttgg gcaatttgt atttggacc aaaagcgca 
      acgacgagg ctctggacg cgtgggaaa tggccgtac 
      tttaccgcc cttcgcacg tggcggctt ggagcaaca 
      ccttggtgc tcctggaca gtatgagac aatacgaca 
      aagtgctag agttgggaa ttaccgccc ttatccaca 
      acccgaaat ccccggccc tgcatttca gtcttgcca 
      acacgctgg ggaaaatcc agcggttcg aaaaagccg 
      aaagtcatt ttctggaag cgatcaatt tgaatccaa 
      acaacgctc tctaccact cgaatttag gggttttat 
      atcacagat ggggtaaag ttgataagg ctcgtgaga 
      tgtataaaa aggcacttc agattgatc cccatatga 
      aaacgaccg aactgaatc ttcgcaaat tggtgaatg 
      ctcaagatt gaggcaggc aataaatac ttttgtatc 
      tgattaact tgtgtggta ctgaacgaa taaaggatt 
      gagagtttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY