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Gene information
General information
TC014151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6771310..6775053
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014151
    iBeetle screen iB_08254

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014151 RA
      MEKSKKYIT ISVAAFICY CNTLWGSFV FDDTEAIVK 
NKDVMPYIP LNEIFRNDF WGSNISLNS SHKSYRPLT 
ILSYRLNVL YSNNKLDAF QFHATNVIL YGLLCLLTI 
PVFELFLRK KKTQKSVDD TAYLSSLLF TVHPIHTEC 
VAGLVGRAD ILCSILFFI VILLYDKAI SKKSFVLLC 
IVVVTTAAA VLCKETAIT VLGVCVVYD LFLLKKQRK 
DWCDIFNIY FFIRTATLV VTGLVILFY RFKIMNFEG 
PTFTSIDNP AAYADKVFI RIFSYNYIY FLNFLLLLW 
PQWLCFDWS MGCIPLIER ISDKRILIV LIFWIITLI 
SVHHLAKKV VENRFLDAK TMALGLIIF PFLPASNLF 
LKVGFVIAE RTLLLPSAG FCLLVVIGL KKIQSFAPS 
HKTTVVVLF YTVCVIFTL RSIQRNFEW LREETLFTS 
ALNVCPLNA KVHYNIAKI AGDQNNKTL ALSEYKKAL 
ELNPNYEQA MNNLANLLR EDRKFGEAE ALLRKALDV 
RPNFAAAWM NLGIVLTNL NRTEEAEHC YKTAIKFRN 
KYPDCYYNL GNLYLDQKR NDEALDAWE MAVLYRPSH 
VAAWSNTLV LLDSMRQYD KVLELGITA LIHNPKSPA 
LHFSLANTL GKIQRFEKA ESHFLEAIN LNPNNALYH 
SNLGVLYHR WGKVDKARE MYKKALQID PHMKTTELN 
LRKLVNAQD
    CDS sequence
    • >TC014151 RA
      atggaaaaa tctaagaaa tacatcaca atttccgta 
gcggcgttt atttgttac tgcaataca ctctggggc 
tcattcgtc ttcgacgac accgaagca atagtcaaa 
aacaaagat gtgatgcct tacattcca ctaaacgag 
atattccga aacgacttt tggggcagc aatatctcg 
cttaacagt agtcacaag tcctataga cctctcact 
attctgtct tatagactg aacgtttta tattccaat 
aataaacta gacgcattc caatttcac gcaaccaat 
gtgattctt tacggactg ttatgttta ctcaccatt 
cccgtgttt gaattgttt ctccgcaaa aagaaaact 
caaaaatcc gttgacgac actgcctat ttatcaagc 
ttgttattc acagtccac cccattcac acagagtgt 
gtcgccgga ctcgtcggc agggccgat attttgtgc 
tccatttta ttcttcatt gtgatatta ttgtatgat 
aaagcaatt agcaaaaaa tcgtttgtg ttactttgt 
atcgttgtg gtgaccaca gcggctgct gtcttatgt 
aaagaaacg gcgataact gttttgggc gtttgtgtt 
gtttatgat ctttttttg ttgaaaaaa caaaggaaa 
gattggtgc gacatattt aatatttat ttttttata 
cgtactgcg actctggtt gtgacagga ttagtcatt 
ttgttttat aggtttaaa attatgaac ttcgaaggc 
cctactttt acgagcatc gataaccca gctgcgtat 
gccgacaaa gtttttatt agaatattt tcctacaac 
tatatttat tttttgaat tttttgctc ttgttgtgg 
ccacagtgg ctgtgtttt gactggtct atggggtgc 
atacctttg attgaaaga atctcggat aaaagaata 
ctgatcgtc ttgattttc tggataatt accctcata 
tctgtgcat catttggct aaaaaagtc gtcgaaaac 
agattcttg gacgctaaa accatggca cttggttta 
ataattttc ccattttta ccagcttca aatttattc 
ctgaaagtg gggtttgtg atagctgaa cgtactcta 
ctattacct agcgctggg ttttgtcta ctggttgtg 
atcggttta aaaaaaatc cagtctttt gctcctagt 
cacaaaacg actgtagtt gtgttattt tacacagtt 
tgtgtcatt tttaccctt cgctcaatt caacgaaac 
ttcgaatgg ctgcgagag gaaacactg ttcacttca 
gctctcaat gtgtgtcct ctcaatgcc aaagtgcac 
tacaacatc gccaaaata gccggcgac caaaacaac 
aaaactttg gctttatcc gagtataaa aaagccctc 
gagctcaac ccgaactac gaacaggcg atgaacaac 
ttggccaac ttattgcgt gaagaccgg aagtttggc 
gaagccgaa gccttgctc cgcaaagca cttgatgtt 
aggccaaat ttcgcggcc gcttggatg aatctgggg 
atagttttg acgaattta aatcggacc gaggaggcc 
gagcattgc tacaaaacc gcgatcaag ttcaggaat 
aaatacccg gattgttat tacaacttg ggcaatttg 
tatttggac caaaagcgc aacgacgag gctctggac 
gcgtgggaa atggccgta ctttaccgc ccttcgcac 
gtggcggct tggagcaac accttggtg ctcctggac 
agtatgaga caatacgac aaagtgcta gagttggga 
attaccgcc cttatccac aacccgaaa tccccggcc 
ctgcatttc agtcttgcc aacacgctg gggaaaatc 
cagcggttc gaaaaagcc gaaagtcat tttctggaa 
gcgatcaat ttgaatcca aacaacgct ctctaccac 
tcgaattta ggggtttta tatcacaga tggggtaaa 
gttgataag gctcgtgag atgtataaa aaggcactt 
cagattgat ccccatatg aaaacgacc gaactgaat 
cttcgcaaa ttggtgaat gctcaagat tga
    mRNA sequence
    • >TC014151 RA
      gcggctgta aatttcaaa cgtggggat tttcagtaa 
cgctttcac gcttctcgc attacaaaa cactttgtg 
aggttaaaa aatttgaca accatcaaa caaatgatg 
aaataaaac aaaatttta aatttctcc aatttatta 
aatgaagcc cgactaaaa tggaaaaat ctaagaaat 
acatcacaa tttccgtag cggcgttta tttgttact 
gcaatacac tctggggct cattcgtct tcgacgaca 
ccgaagcaa tagtcaaaa acaaagatg tgatgcctt 
acattccac taaacgaga tattccgaa acgactttt 
ggggcagca atatctcgc ttaacagta gtcacaagt 
cctatagac ctctcacta ttctgtctt atagactga 
acgttttat attccaata ataaactag acgcattcc 
aatttcacg caaccaatg tgattcttt acggactgt 
tatgtttac tcaccattc ccgtgtttg aattgtttc 
tccgcaaaa agaaaactc aaaaatccg ttgacgaca 
ctgcctatt tatcaagct tgttattca cagtccacc 
ccattcaca cagagtgtg tcgccggac tcgtcggca 
gggccgata ttttgtgct ccattttat tcttcattg 
tgatattat tgtatgata aagcaatta gcaaaaaat 
cgtttgtgt tactttgta tcgttgtgg tgaccacag 
cggctgctg tcttatgta aagaaacgg cgataactg 
ttttgggcg tttgtgttg tttatgatc tttttttgt 
tgaaaaaac aaaggaaag attggtgcg acatattta 
atatttatt tttttatac gtactgcga ctctggttg 
tgacaggat tagtcattt tgttttata ggtttaaaa 
ttatgaact tcgaaggcc ctactttta cgagcatcg 
ataacccag ctgcgtatg ccgacaaag tttttatta 
gaatatttt cctacaact atatttatt ttttgaatt 
ttttgctct tgttgtggc cacagtggc tgtgttttg 
actggtcta tggggtgca tacctttga ttgaaagaa 
tctcggata aaagaatac tgatcgtct tgattttct 
ggataatta ccctcatat ctgtgcatc atttggcta 
aaaaagtcg tcgaaaaca gattcttgg acgctaaaa 
ccatggcac ttggtttaa taattttcc catttttac 
cagcttcaa atttattcc tgaaagtgg ggtttgtga 
tagctgaac gtactctac tattaccta gcgctgggt 
tttgtctac tggttgtga tcggtttaa aaaaaatcc 
agtcttttg ctcctagtc acaaaacga ctgtagttg 
tgttatttt acacagttt gtgtcattt ttacccttc 
gctcaattc aacgaaact tcgaatggc tgcgagagg 
aaacactgt tcacttcag ctctcaatg tgtgtcctc 
tcaatgcca aagtgcact acaacatcg ccaaaatag 
ccggcgacc aaaacaaca aaactttgg ctttatccg 
agtataaaa aagccctcg agctcaacc cgaactacg 
aacaggcga tgaacaact tggccaact tattgcgtg 
aagaccgga agtttggcg aagccgaag ccttgctcc 
gcaaagcac ttgatgtta ggccaaatt tcgcggccg 
cttggatga atctgggga tagttttga cgaatttaa 
atcggaccg aggaggccg agcattgct acaaaaccg 
cgatcaagt tcaggaata aatacccgg attgttatt 
acaacttgg gcaatttgt atttggacc aaaagcgca 
acgacgagg ctctggacg cgtgggaaa tggccgtac 
tttaccgcc cttcgcacg tggcggctt ggagcaaca 
ccttggtgc tcctggaca gtatgagac aatacgaca 
aagtgctag agttgggaa ttaccgccc ttatccaca 
acccgaaat ccccggccc tgcatttca gtcttgcca 
acacgctgg ggaaaatcc agcggttcg aaaaagccg 
aaagtcatt ttctggaag cgatcaatt tgaatccaa 
acaacgctc tctaccact cgaatttag gggttttat 
atcacagat ggggtaaag ttgataagg ctcgtgaga 
tgtataaaa aggcacttc agattgatc cccatatga 
aaacgaccg aactgaatc ttcgcaaat tggtgaatg 
ctcaagatt gaggcaggc aataaatac ttttgtatc 
tgattaact tgtgtggta ctgaacgaa taaaggatt 
gagagtttt
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