| General information | |||
| TC014151 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 6771310..6775053 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014151 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08254 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014151 RA
MEKSKKYIT ISVAAFICY CNTLWGSFV FDDTEAIVK NKDVMPYIP LNEIFRNDF WGSNISLNS SHKSYRPLT ILSYRLNVL YSNNKLDAF QFHATNVIL YGLLCLLTI PVFELFLRK KKTQKSVDD TAYLSSLLF TVHPIHTEC VAGLVGRAD ILCSILFFI VILLYDKAI SKKSFVLLC IVVVTTAAA VLCKETAIT VLGVCVVYD LFLLKKQRK DWCDIFNIY FFIRTATLV VTGLVILFY RFKIMNFEG PTFTSIDNP AAYADKVFI RIFSYNYIY FLNFLLLLW PQWLCFDWS MGCIPLIER ISDKRILIV LIFWIITLI SVHHLAKKV VENRFLDAK TMALGLIIF PFLPASNLF LKVGFVIAE RTLLLPSAG FCLLVVIGL KKIQSFAPS HKTTVVVLF YTVCVIFTL RSIQRNFEW LREETLFTS ALNVCPLNA KVHYNIAKI AGDQNNKTL ALSEYKKAL ELNPNYEQA MNNLANLLR EDRKFGEAE ALLRKALDV RPNFAAAWM NLGIVLTNL NRTEEAEHC YKTAIKFRN KYPDCYYNL GNLYLDQKR NDEALDAWE MAVLYRPSH VAAWSNTLV LLDSMRQYD KVLELGITA LIHNPKSPA LHFSLANTL GKIQRFEKA ESHFLEAIN LNPNNALYH SNLGVLYHR WGKVDKARE MYKKALQID PHMKTTELN LRKLVNAQD
- >TC014151 RA
atggaaaaa tctaagaaa tacatcaca atttccgta gcggcgttt atttgttac tgcaataca ctctggggc tcattcgtc ttcgacgac accgaagca atagtcaaa aacaaagat gtgatgcct tacattcca ctaaacgag atattccga aacgacttt tggggcagc aatatctcg cttaacagt agtcacaag tcctataga cctctcact attctgtct tatagactg aacgtttta tattccaat aataaacta gacgcattc caatttcac gcaaccaat gtgattctt tacggactg ttatgttta ctcaccatt cccgtgttt gaattgttt ctccgcaaa aagaaaact caaaaatcc gttgacgac actgcctat ttatcaagc ttgttattc acagtccac cccattcac acagagtgt gtcgccgga ctcgtcggc agggccgat attttgtgc tccatttta ttcttcatt gtgatatta ttgtatgat aaagcaatt agcaaaaaa tcgtttgtg ttactttgt atcgttgtg gtgaccaca gcggctgct gtcttatgt aaagaaacg gcgataact gttttgggc gtttgtgtt gtttatgat ctttttttg ttgaaaaaa caaaggaaa gattggtgc gacatattt aatatttat ttttttata cgtactgcg actctggtt gtgacagga ttagtcatt ttgttttat aggtttaaa attatgaac ttcgaaggc cctactttt acgagcatc gataaccca gctgcgtat gccgacaaa gtttttatt agaatattt tcctacaac tatatttat tttttgaat tttttgctc ttgttgtgg ccacagtgg ctgtgtttt gactggtct atggggtgc atacctttg attgaaaga atctcggat aaaagaata ctgatcgtc ttgattttc tggataatt accctcata tctgtgcat catttggct aaaaaagtc gtcgaaaac agattcttg gacgctaaa accatggca cttggttta ataattttc ccattttta ccagcttca aatttattc ctgaaagtg gggtttgtg atagctgaa cgtactcta ctattacct agcgctggg ttttgtcta ctggttgtg atcggttta aaaaaaatc cagtctttt gctcctagt cacaaaacg actgtagtt gtgttattt tacacagtt tgtgtcatt tttaccctt cgctcaatt caacgaaac ttcgaatgg ctgcgagag gaaacactg ttcacttca gctctcaat gtgtgtcct ctcaatgcc aaagtgcac tacaacatc gccaaaata gccggcgac caaaacaac aaaactttg gctttatcc gagtataaa aaagccctc gagctcaac ccgaactac gaacaggcg atgaacaac ttggccaac ttattgcgt gaagaccgg aagtttggc gaagccgaa gccttgctc cgcaaagca cttgatgtt aggccaaat ttcgcggcc gcttggatg aatctgggg atagttttg acgaattta aatcggacc gaggaggcc gagcattgc tacaaaacc gcgatcaag ttcaggaat aaatacccg gattgttat tacaacttg ggcaatttg tatttggac caaaagcgc aacgacgag gctctggac gcgtgggaa atggccgta ctttaccgc ccttcgcac gtggcggct tggagcaac accttggtg ctcctggac agtatgaga caatacgac aaagtgcta gagttggga attaccgcc cttatccac aacccgaaa tccccggcc ctgcatttc agtcttgcc aacacgctg gggaaaatc cagcggttc gaaaaagcc gaaagtcat tttctggaa gcgatcaat ttgaatcca aacaacgct ctctaccac tcgaattta ggggtttta tatcacaga tggggtaaa gttgataag gctcgtgag atgtataaa aaggcactt cagattgat ccccatatg aaaacgacc gaactgaat cttcgcaaa ttggtgaat gctcaagat tga
- >TC014151 RA
gcggctgta aatttcaaa cgtggggat tttcagtaa cgctttcac gcttctcgc attacaaaa cactttgtg aggttaaaa aatttgaca accatcaaa caaatgatg aaataaaac aaaatttta aatttctcc aatttatta aatgaagcc cgactaaaa tggaaaaat ctaagaaat acatcacaa tttccgtag cggcgttta tttgttact gcaatacac tctggggct cattcgtct tcgacgaca ccgaagcaa tagtcaaaa acaaagatg tgatgcctt acattccac taaacgaga tattccgaa acgactttt ggggcagca atatctcgc ttaacagta gtcacaagt cctatagac ctctcacta ttctgtctt atagactga acgttttat attccaata ataaactag acgcattcc aatttcacg caaccaatg tgattcttt acggactgt tatgtttac tcaccattc ccgtgtttg aattgtttc tccgcaaaa agaaaactc aaaaatccg ttgacgaca ctgcctatt tatcaagct tgttattca cagtccacc ccattcaca cagagtgtg tcgccggac tcgtcggca gggccgata ttttgtgct ccattttat tcttcattg tgatattat tgtatgata aagcaatta gcaaaaaat cgtttgtgt tactttgta tcgttgtgg tgaccacag cggctgctg tcttatgta aagaaacgg cgataactg ttttgggcg tttgtgttg tttatgatc tttttttgt tgaaaaaac aaaggaaag attggtgcg acatattta atatttatt tttttatac gtactgcga ctctggttg tgacaggat tagtcattt tgttttata ggtttaaaa ttatgaact tcgaaggcc ctactttta cgagcatcg ataacccag ctgcgtatg ccgacaaag tttttatta gaatatttt cctacaact atatttatt ttttgaatt ttttgctct tgttgtggc cacagtggc tgtgttttg actggtcta tggggtgca tacctttga ttgaaagaa tctcggata aaagaatac tgatcgtct tgattttct ggataatta ccctcatat ctgtgcatc atttggcta aaaaagtcg tcgaaaaca gattcttgg acgctaaaa ccatggcac ttggtttaa taattttcc catttttac cagcttcaa atttattcc tgaaagtgg ggtttgtga tagctgaac gtactctac tattaccta gcgctgggt tttgtctac tggttgtga tcggtttaa aaaaaatcc agtcttttg ctcctagtc acaaaacga ctgtagttg tgttatttt acacagttt gtgtcattt ttacccttc gctcaattc aacgaaact tcgaatggc tgcgagagg aaacactgt tcacttcag ctctcaatg tgtgtcctc tcaatgcca aagtgcact acaacatcg ccaaaatag ccggcgacc aaaacaaca aaactttgg ctttatccg agtataaaa aagccctcg agctcaacc cgaactacg aacaggcga tgaacaact tggccaact tattgcgtg aagaccgga agtttggcg aagccgaag ccttgctcc gcaaagcac ttgatgtta ggccaaatt tcgcggccg cttggatga atctgggga tagttttga cgaatttaa atcggaccg aggaggccg agcattgct acaaaaccg cgatcaagt tcaggaata aatacccgg attgttatt acaacttgg gcaatttgt atttggacc aaaagcgca acgacgagg ctctggacg cgtgggaaa tggccgtac tttaccgcc cttcgcacg tggcggctt ggagcaaca ccttggtgc tcctggaca gtatgagac aatacgaca aagtgctag agttgggaa ttaccgccc ttatccaca acccgaaat ccccggccc tgcatttca gtcttgcca acacgctgg ggaaaatcc agcggttcg aaaaagccg aaagtcatt ttctggaag cgatcaatt tgaatccaa acaacgctc tctaccact cgaatttag gggttttat atcacagat ggggtaaag ttgataagg ctcgtgaga tgtataaaa aggcacttc agattgatc cccatatga aaacgaccg aactgaatc ttcgcaaat tggtgaatg ctcaagatt gaggcaggc aataaatac ttttgtatc tgattaact tgtgtggta ctgaacgaa taaaggatt gagagtttt
