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Gene information
General information
TC033086
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014145
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6617420..6619425
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033086
    iBeetle screen iB_08252

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033086 RA
      MPYQKNPSI ISNPKPVNI FYVWYTELC RRLNSTPAP 
      VVKPAKPKC QTVLNFVAD RLKVEEWNP VISALRHDT 
      SLHVIAIKS RLGNCQFLH DVDTEEKLR QMKRRSGSL 
      WTAFILKSL LKSLSCTIR NTQVLTCLE FDGLPMFAQ 
      YLEPLMKAL QKNKTLKNL TLSRCRICD SGCQIVCAY 
      VRFAPNIEV LNLSGCGLT AESGEHLAK LIKYQQINR 
      YCESWHNSL RYADPNIQV MRGIKRITL NGNPLIGDS 
      GLNFILNEL DDDLWIKAL DMQKCGITE SMAMRIIDL 
      VEYSQSLEI ADFRLNEDL TDSTLEKIL NVLKQKYVE 
      GQSEYQWCN TSLSTACET FIDTMSTFT STKTPHVYK 
      AKSTPFRRY ESKITLTER PVRRAKTSE NIHNQAKPD 
      DVNIEKKVM ELSNLLKEE ILKRKETEK INEELKRRL 
      DEFSKGVQD EKPNVDVIN NVVLNRKDS GPAEMLNQI 
      CRKNGVKKK NGFKNGATT NGYHNDNGF KFNNDVAFT 
      IFEKLVGKD SDDRSLDED SLLDYVREG KKEKDDNRN 
      DCDMSDSQI SLFKFMENL KNNHNLPMK FMNRSNTSK 
      YYITDKHVK RKA
    CDS sequence
    • >TC033086 RA
      atgccatac cagaaaaat ccatctatt atcagtaat 
      ccaaaaccg gtgaacatc ttttacgtg tggtacact 
      gaactttgt cgccgttta aacagcacc ccagctcct 
      gtggtaaaa cctgcgaag cctaaatgt caaacagta 
      ttgaatttt gtagctgac cgattaaaa gtagaagag 
      tggaatccg gtaataagt gctttgagg cacgatacc 
      agtcttcat gtcattgca attaaaagt agattaggt 
      aactgccag tttctgcat gacgtcgac actgaggaa 
      aaactgcgc cagatgaag aggcgttct ggctcgctc 
      tggaccgcc tttatcctg aaatcgctg ctcaagtcc 
      ttgtcctgc accatccga aacacccaa gttttgacg 
      tgtctcgag tttgatgga ctgcccatg tttgcacaa 
      tacctggaa ccactcatg aaggcgctc cagaagaac 
      aaaactttg aaaaatcta actctgagc cggtgccgg 
      atttgcgac tccgggtgt caaatcgtg tgcgcttac 
      gtacggttt gccccgaac atcgaagtg ctgaatctc 
      agcggatgt gggctgacg gctgaaagt ggagaacat 
      ttggccaaa ctgattaaa tatcagcaa atcaacaga 
      tactgcgag agctggcac aactccttg aggtatgcg 
      gatcccaac attcaagtg atgcgaggt attaaaagg 
      ataactttg aacggcaac cctttgatc ggagatagt 
      ggtctcaat tttattttg aacgagctg gatgatgat 
      ttgtggatc aaagctttg gacatgcaa aaatgtggg 
      ataactgaa agcatggcc atgaggatc atcgacctc 
      gttgaatac agtcagtcg ctggaaatc gcagatttt 
      agactaaac gaggacttg actgactcg actttggag 
      aaaattcta aatgttctt aaacagaaa tatgtcgag 
      ggccagtcg gaataccag tggtgtaac actagctta 
      agcacagct tgtgaaaca tttatagac acaatgtcc 
      actttcact tctaccaaa accccgcat gtctacaaa 
      gccaagtcc acacctttt agacgctac gagtccaaa 
      atcacctta accgagaga cctgtcaga cgagccaaa 
      acctctgag aacattcac aaccaagcc aagccagac 
      gatgtgaac attgaaaag aaggtgatg gaactaagc 
      aacttactg aaagaagaa atactgaaa cgcaaggaa 
      actgaaaaa atcaacgag gaactaaag cgaagactc 
      gatgagttt agcaaagga gtgcaagac gagaaacct 
      aacgtagat gtaattaat aacgtggtt ttgaaccgt 
      aaagactcc ggtcctgcg gaaatgctc aatcaaatt 
      tgtcgaaaa aatggggtg aagaaaaag aacgggttc 
      aaaaacggc gctacgaca aacggatac cacaacgac 
      aacggattc aaattcaac aacgacgtg gcatttaca 
      attttcgaa aagttagtt ggtaaagat agtgatgac 
      agaagcttg gacgaggac agtttgctc gattacgta 
      cgggaaggg aagaaggag aaagatgat aacagaaat 
      gactgcgat atgagtgac agtcagatt tcgttattt 
      aagtttatg gaaaattta aagaacaac cacaacttg 
      cctatgaaa tttatgaat cgttcaaac acttcaaaa 
      tattatatt acggataaa cacgtaaaa aggaaggcg 
      tga
    mRNA sequence
    • >TC033086 RA
      tgtcacaaa aataattta tttataaat gtcaatcac 
      ttattttgt tgtttgtca agtttataa aatcgtcca 
      ttcatgttg atttttgtt ctaaaacct cgaatcgtc 
      caaaattaa gctgaagat ttgatctta aaatgccat 
      accagaaaa atccatcta ttatcagta atccaaaac 
      cggtgaaca tcttttacg tgtggtaca ctgaacttt 
      gtcgccgtt taaacagca ccccagctc ctgtggtaa 
      aacctgcga agcctaaat gtcaaacag tattgaatt 
      ttgtagctg accgattaa aagtagaag agtggaatc 
      cggtaataa gtgctttga ggcacgata ccagtcttc 
      atgtcattg caattaaaa gtagattag gtaactgcc 
      agtttctgc atgacgtcg acactgagg aaaaactgc 
      gccagatga agaggcgtt ctggctcgc tctggaccg 
      cctttatcc tgaaatcgc tgctcaagt ccttgtcct 
      gcaccatcc gaaacaccc aagttttga cgtgtctcg 
      agtttgatg gactgccca tgtttgcac aatacctgg 
      aaccactca tgaaggcgc tccagaaga acaaaactt 
      tgaaaaatc taactctga gccggtgcc ggatttgcg 
      actccgggt gtcaaatcg tgtgcgctt acgtacggt 
      ttgccccga acatcgaag tgctgaatc tcagcggat 
      gtgggctga cggctgaaa gtggagaac atttggcca 
      aactgatta aatatcagc aaatcaaca gatactgcg 
      agagctggc acaactcct tgaggtatg cggatccca 
      acattcaag tgatgcgag gtattaaaa ggataactt 
      tgaacggca accctttga tcggagata gtggtctca 
      attttattt tgaacgagc tggatgatg atttgtgga 
      tcaaagctt tggacatgc aaaaatgtg ggataactg 
      aaagcatgg ccatgagga tcatcgacc tcgttgaat 
      acagtcagt cgctggaaa tcgcagatt ttagactaa 
      acgaggact tgactgact cgactttgg agaaaattc 
      taaatgttc ttaaacaga aatatgtcg agggccagt 
      cggaatacc agtggtgta acactagct taagcacag 
      cttgtgaaa catttatag acacaatgt ccactttca 
      cttctacca aaaccccgc atgtctaca aagccaagt 
      ccacacctt ttagacgct acgagtcca aaatcacct 
      taaccgaga gacctgtca gacgagcca aaacctctg 
      agaacattc acaaccaag ccaagccag acgatgtga 
      acattgaaa agaaggtga tggaactaa gcaacttac 
      tgaaagaag aaatactga aacgcaagg aaactgaaa 
      aaatcaacg aggaactaa agcgaagac tcgatgagt 
      ttagcaaag gagtgcaag acgagaaac ctaacgtag 
      atgtaatta ataacgtgg ttttgaacc gtaaagact 
      ccggtcctg cggaaatgc tcaatcaaa tttgtcgaa 
      aaaatgggg tgaagaaaa agaacgggt tcaaaaacg 
      gcgctacga caaacggat accacaacg acaacggat 
      tcaaattca acaacgacg tggcattta caattttcg 
      aaaagttag ttggtaaag atagtgatg acagaagct 
      tggacgagg acagtttgc tcgattacg tacgggaag 
      ggaagaagg agaaagatg ataacagaa atgactgcg 
      atatgagtg acagtcaga tttcgttat ttaagttta 
      tggaaaatt taaagaaca accacaact tgcctatga 
      aatttatga atcgttcaa acacttcaa aatattata 
      ttacggata aacacgtaa aaaggaagg cgtgatcgt 
      gtgaagaat gtctctatt aaaacttaa gtttttttg 
      cgcaactgt aacaaacaa cgtatattt ctaaattgg 
      aagtggaat aaatataat tatacacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY