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Gene information
General information
TC014133 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6422565..6436655
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014133
    iBeetle screen iB_08251

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014133 RA
      MVLFTEKGG PKIKHTEAA KKKIASRRG TRVVLPSDP 
      RFSKLRKQK VKLFTAAQK WWSKASIKY AFSHPHVTS 
      RLEKLMGSN LVSLTDRDY MQKLQERIL EDYAESMDK 
      RIKVRENEE MERVKNLVL SGRIPLHEA PESMADHPI 
      MMIERHCKK LIAARRAKI KIPKVHIPT HLYSDDTPD 
      PPSGLSIEN GHIFRKPYE KLCNPVSRF LPVEEFPFY 
      DFDDSDEEE EEEDEGEGE IDTTVQKIV GGEAAPGGE 
      GVEEEEEVT DAYMFTRAE YDRLKYESP LIKQLRRCQ 
      IVEELYALA DEIIGILKT LEPESAAVR TTSEDTLFS 
      VTTSTATQE KGSDRRSSQ GSQRSQAVS H
    CDS sequence
    • >TC014133 RA
      atggtgttg ttcactgaa aaagggggt ccaaaaatt 
      aaacatact gaagcagct aagaaaaaa atagcgtca 
      cgtcggggt accagagtt gtgcttcca agcgacccc 
      agattttcc aaacttaga aaacaaaaa gttaaatta 
      tttactgcg gctcaaaaa tggtggtca aaagcgagt 
      atcaaatac gcattcagt catcctcat gtgacatca 
      cgactggaa aagctaatg ggctccaat ttggtttcc 
      ttaacggac agagattat atgcaaaaa cttcaggag 
      cgcattctg gaagactat gccgagtcg atggacaag 
      aggataaaa gtccgtgaa aacgaagaa atggagcgg 
      gtgaaaaat ctggtcttg agtggaaga attccttta 
      catgaagcg cctgaaagt atggctgat catccgatc 
      atgatgatt gaaagacat tgcaagaag ttaatagcc 
      gctcgtcga gccaaaatt aaaatacct aaagtgcac 
      attcctacg cacctttat tccgacgat acacctgat 
      ccaccttct ggcctttct atagaaaat ggccacatt 
      ttcagaaaa ccatacgaa aaattatgc aacccagtc 
      agtcggttc ctacctgtt gaagaattc ccattctat 
      gattttgat gactctgat gaagaggaa gaagaggag 
      gatgaaggt gaaggtgaa attgataca acagtgcag 
      aaaatagtt ggaggagaa gctgctcct ggtggcgaa 
      ggtgtagag gaggaagaa gaagttaca gatgcatac 
      atgttcaca cgtgctgaa tatgacagg cttaaatat 
      gagagtccg ctaataaag caactacga cgctgccag 
      attgtggaa gaattatat gccttggcc gacgaaata 
      attggtatt ttgaagact ctggaacct gagtcagcc 
      gcagttcgc accacaagt gaagacaca cttttctcg 
      gttacaaca agcacagcg acgcaagag aaaggctcc 
      gaccgacga agttcgcaa ggctcacaa cgttcacaa 
      gctgtctca cactga
    mRNA sequence
    • >TC014133 RA
      cagttatta aaatttgtg acaaaaatg tcagtgtag 
      ttttaaacc ttttcgaaa ttcaaaatt actgtagcg 
      ccgttttcg atgtataaa ttattttaa tgtaaagtg 
      aacagtttt tttgacgtc tcgtgtctg tatgtaatt 
      tgtaacggg caaaatggt gttgttcac tgaaaaagg 
      gggtccaaa aattaaaca tactgaagc agctaagaa 
      aaaaatagc gtcacgtcg gggtaccag agttgtgct 
      tccaagcga ccccagatt ttccaaact tagaaaaca 
      aaaagttaa attatttac tgcggctca aaaatggtg 
      gtcaaaagc gagtatcaa atacgcatt cagtcatcc 
      tcatgtgac atcacgact ggaaaagct aatgggctc 
      caatttggt ttccttaac ggacagaga ttatatgca 
      aaaacttca ggagcgcat tctggaaga ctatgccga 
      gtcgatgga caagaggat aaaagtccg tgaaaacga 
      agaaatgga gcgggtgaa aaatctggt cttgagtgg 
      aagaattcc tttacatga agcgcctga aagtatggc 
      tgatcatcc gatcatgat gattgaaag acattgcaa 
      gaagttaat agccgctcg tcgagccaa aattaaaat 
      acctaaagt gcacattcc tacgcacct ttattccga 
      cgatacacc tgatccacc ttctggcct ttctataga 
      aaatggcca cattttcag aaaaccata cgaaaaatt 
      atgcaaccc agtcagtcg gttcctacc tgttgaaga 
      attcccatt ctatgattt tgatgactc tgatgaaga 
      ggaagaaga ggaggatga aggtgaagg tgaaattga 
      tacaacagt gcagaaaat agttggagg agaagctgc 
      tcctggtgg cgaaggtgt agaggagga agaagaagt 
      tacagatgc atacatgtt cacacgtgc tgaatatga 
      caggcttaa atatgagag tccgctaat aaagcaact 
      acgacgctg ccagattgt ggaagaatt atatgcctt 
      ggccgacga aataattgg tattttgaa gactctgga 
      acctgagtc agccgcagt tcgcaccac aagtgaaga 
      cacactttt ctcggttac aacaagcac agcgacgca 
      agagaaagg ctccgaccg acgaagttc gcaaggctc 
      acaacgttc acaagctgt ctcacactg aagagacta 
      aattatttt cttgttgta agtgtaaac tattaaatg 
      tagcgttat gtaaaaata tggtcgttc tgttttgta 
      aaatgtaat taaacgtct gtctcgagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY