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Gene information
General information
TC013687 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6038621..6040421
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013687
    iBeetle screen iB_08248

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013687 RA
      MGAIVSLIR LNIRQREVE THRCSIKVD ESMSFLPPL 
      RRSASCFQR FRRAFRKSI LISERNPQS ADYFRSKAE 
      ILRIRKEHV ISKSWIIHP FSIFRAYYE MWMTVVWIL 
      VLFLMPADA AFDLLNPMH QHLLQEERK DVPNSIDPL 
      SLCLRLLCI VDILMTFFT GFPVKKSRK VVMQQWKIA 
      KKYVFSIYF ITDLLASLP LEFVISDHN TQSLVDVFS 
      FLRIIRLPT LYFYITRSL DVLGAKSLT IHILKNILV 
      NYLVFHWFA CFQYMIPQI RFYTYGEIL AESWVERTD 
      IPELPFISK YVICIFRAA GCLLCITFE EITIDVWEE 
      KLLALASFI FGKVYVFYI TVLLLNFLL RQRSLEVKY 
      FETISQVQA YMSQKQLPM SMQTRLLQF YDYKYNKKF 
      FKERGIASL LSEKLKGEI NLNVCSKLV QNVELLAHL 
      PQQTLEQVV TNLKPEIYL SNDIIIKAG SVGDCMYFL 
      ASGTVGVWT PSGKEVCHL HDGAYFGEI SLVFKDKRR 
      TANIVAVEI CEVYKLDRR AFKNCFKTN TELYKMLED 
      VANERMEIT QMYEDVHTK TFMELSH
    CDS sequence
    • >TC013687 RA
      atgggcgca attgtgtct ctaatccgc ctcaatatt 
      cgccaaaga gaagtggaa actcatcga tgcagcatc 
      aaggtggac gaatccatg agtttcttg cctcctctc 
      agaagaagc gcaagttgt ttccaacgc ttccgacgt 
      gcatttagg aaaagtata ctcatatcg gagcgcaat 
      ccacagagt gccgactat ttcaggagc aaagctgaa 
      attttgcga ataagaaaa gagcatgtg atctcaaaa 
      tcgtggata atccatccg tttagtatt tttcgggcg 
      tactacgaa atgtggatg acagtagtc tggatttta 
      gtgcttttt ctaatgccg gctgatgca gcctttgat 
      ttgttaaac cctatgcac caacacttg ctccaggag 
      gaacggaag gacgtccca aactcaatt gacccgtta 
      agcctgtgt ttgcgtctg ttgtgcatt gtcgatata 
      ctaatgacg tttttcacc ggttttccc gtcaaaaaa 
      tcgcggaaa gttgtaatg cagcagtgg aaaattgcc 
      aaaaagtac gtttttagc atctatttt attaccgat 
      cttttggct tctctgcct ctggagttc gtgatcagc 
      gatcacaac actcaatct ttagtcgat gtgttctca 
      tttttgagg ataatacga ttaccgacc ctgtatttt 
      tacataaca aggagtctc gatgttttg ggggcaaaa 
      agtctcacg attcacatt ctgaagaac attcttgtc 
      aattatctg gtgttccat tggttcgcc tgctttcag 
      tacatgata ccgcagatt cgtttttac acttacgga 
      gaaattttg gccgagtct tgggttgaa agaactgat 
      attcccgag ttacctttt atttcaaag tacgttatt 
      tgcattttt cgagccgcg ggatgccta ctttgtatc 
      acttttgag gaaattaca atcgacgtg tgggaagag 
      aagctttta gcgctcgct agtttcatt ttcggaaaa 
      gtttacgtt ttttacatc accgtcctg cttttgaat 
      ttcttgctc agacaaagg tctcttgag gtcaaatat 
      tttgagacc atcagccag gttcaagcc tacatgagc 
      caaaaacaa ctaccaatg tcgatgcaa acgcgactt 
      ttgcagttc tacgactac aagtacaac aagaagttt 
      ttcaaagaa agggggatt gctagtctg ctttcggag 
      aaactgaaa ggagagata aacctgaat gtgtgtagt 
      aaattggtg cagaacgtg gaactgttg gcccatctt 
      ccacaacaa accttggaa caggtcgtc acgaatttg 
      aagcctgag atatacttg tccaacgat attataatt 
      aaggcgggt tctgtgggt gattgtatg tactttttg 
      gctagtggg acggtggga gtgtggaca ccttctggc 
      aaagaagtg tgtcatctg catgatggt gcctacttt 
      ggagagatc tccctggtt ttcaaggat aaaaggaga 
      actgccaat atcgtggct gttgaaatt tgcgaagtc 
      tacaaactg gacaggagg gctttcaag aactgtttc 
      aaaaccaat acggagctg tacaaaatg cttgaagat 
      gtggccaat gagagaatg gaaatcaca caaatgtat 
      gaagacgtc cataccaaa actttcatg gagttgagt 
      cactga
    mRNA sequence
    • >TC013687 RA
      tggattctc aaaatgggc gcaattgtg tctctaatc 
      cgcctcaat attcgccaa agagaagtg gaaactcat 
      cgatgcagc atcaaggtg gacgaatcc atgagtttc 
      ttgcctcct ctcagaaga agcgcaagt tgtttccaa 
      cgcttccga cgtgcattt aggaaaagt atactcata 
      tcggagcgc aatccacag agtgccgac tatttcagg 
      agcaaagct gaaattttg cgaataaga aaagagcat 
      gtgatctca aaatcgtgg ataatccat ccgtttagt 
      atttttcgg gcgtactac gaaatgtgg atgacagta 
      gtctggatt ttagtgctt tttctaatg ccggctgat 
      gcagccttt gatttgtta aaccctatg caccaacac 
      ttgctccag gaggaacgg aaggacgtc ccaaactca 
      attgacccg ttaagcctg tgtttgcgt ctgttgtgc 
      attgtcgat atactaatg acgtttttc accggtttt 
      cccgtcaaa aaatcgcgg aaagttgta atgcagcag 
      tggaaaatt gccaaaaag tacgttttt agcatctat 
      tttattacc gatcttttg gcttctctg cctctggag 
      ttcgtgatc agcgatcac aacactcaa tctttagtc 
      gatgtgttc tcatttttg aggataata cgattaccg 
      accctgtat ttttacata acaaggagt ctcgatgtt 
      ttgggggca aaaagtctc acgattcac attctgaag 
      aacattctt gtcaattat ctggtgttc cattggttc 
      gcctgcttt cagtacatg ataccgcag attcgtttt 
      tacacttac ggagaaatt ttggccgag tcttgggtt 
      gaaagaact gatattccc gagttacct tttatttca 
      aagtacgtt atttgcatt tttcgagcc gcgggatgc 
      ctactttgt atcactttt gaggaaatt acaatcgac 
      gtgtgggaa gagaagctt ttagcgctc gctagtttc 
      attttcgga aaagtttac gttttttac atcaccgtc 
      ctgcttttg aatttcttg ctcagacaa aggtctctt 
      gaggtcaaa tattttgag accatcagc caggttcaa 
      gcctacatg agccaaaaa caactacca atgtcgatg 
      caaacgcga cttttgcag ttctacgac tacaagtac 
      aacaagaag tttttcaaa gaaaggggg attgctagt 
      ctgctttcg gagaaactg aaaggagag ataaacctg 
      aatgtgtgt agtaaattg gtgcagaac gtggaactg 
      ttggcccat cttccacaa caaaccttg gaacaggtc 
      gtcacgaat ttgaagcct gagatatac ttgtccaac 
      gatattata attaaggcg ggttctgtg ggtgattgt 
      atgtacttt ttggctagt gggacggtg ggagtgtgg 
      acaccttct ggcaaagaa gtgtgtcat ctgcatgat 
      ggtgcctac tttggagag atctccctg gttttcaag 
      gataaaagg agaactgcc aatatcgtg gctgttgaa 
      atttgcgaa gtctacaaa ctggacagg agggctttc 
      aagaactgt ttcaaaacc aatacggag ctgtacaaa 
      atgcttgaa gatgtggcc aatgagaga atggaaatc 
      acacaaatg tatgaagac gtccatacc aaaactttc 
      atggagttg agtcactga gtgaatact tttgcctgg 
      gattttgtt gctgttata attgtattt ttctgttat 
      gtacgagta attacctaa ataaagttt acacgtagt 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY