General information | |||
TC013711 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 5474656..5477008 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC013711 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08240 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC013711 RA
MSFETLQIE KICRTCLSE EGDMRSVFS IDESIGETM RLFEMLMSC AAVQVLEND GLPNQVCLQ CVHYITRAF SFKQLCERS DATLRQLLG KPMQATFME LKPLLSSDQ HEPSFNDVI NTVTENIQA VTDSVNEAF SSAVATVNS IDVGDSFKS EGSQDMKMK LEFVDNDVL GIDPDSCGS DAESKINEL RAKRESKKK KIRDDTEQP IYPCEECTQ CFTTMIDLK AHAKTHPKI SRHICKICN QGFASASTL CRHMKVHDG AKRHLCSEC GKGFARSDD LTRHIRTHT GEKPFPCKL CGKSFAQSF RLLEHMRAH ANEKSFICS VCGKAFSRY TSLAAHNKT HSGIKSHAC GVCGKRLCG SGSLAMHMK THTGVKDHI CPYCGKGFT TPSNLTIHK RTHTGERPY VCNECGKGF PDPSRLTVH VRSHSGEKP YVCSVCGRG CVSSSQLKK HRRIHTGEK PYQCNLCPK AFPRSEDLR IHIKTHTGD RNHICPFCK KGFYQASTL KVHLRIHTG EKPYTCNIC NKAFSQTGP LSTHMKTHS
- >TC013711 RA
atgtctttc gaaacactc caaatcgaa aagatatgt agaacctgc ctttcggaa gaaggtgac atgcgttcg gtatttagc attgacgaa tctatcggc gaaacaatg cgtttgttt gaaatgtta atgtcttgt gctgcagtg caggttttg gaaaacgac ggattgccc aaccaagtt tgtttacaa tgtgttcat tacataact cgtgctttt tcatttaaa caattatgc gagagatca gatgcaacg ttgcggcaa ctcctaggc aaacccatg caggccact tttatggaa ttaaaacca ttattgtct agtgatcaa cacgaacct tcatttaac gatgttata aataccgtc acggaaaat atacaggcc gtaacagat tccgtaaat gaggctttt agtagcgca gttgcaaca gtgaatagc atagacgtt ggggattct ttcaagtcg gaagggtcc caagacatg aaaatgaaa ctggagttt gtggacaat gacgtgtta ggaattgat ccagattcg tgtggtagc gatgccgaa agcaaaata aacgaactg agagctaaa agagaatca aagaaaaag aaaattaga gacgacact gaacaaccg atttatcca tgtgaggaa tgcactcag tgtttcact acaatgatc gatttgaag gcccatgct aaaacacat ccgaaaatt agcagacac atttgtaaa atctgtaat caaggtttc gcaagtgcg agcactttg tgtcgacac atgaaagta catgatggc gcaaaacgg catttgtgt agtgagtgt ggcaaaggt tttgcgaga tcggatgat cttacaaga catataaga acacacact ggtgaaaag ccttttcct tgtaaatta tgcggcaaa tcgtttgct caatctttt cgtctgttg gagcatatg agggcgcat gcgaacgaa aaaagtttc atttgttca gtttgtggc aaagccttt tcacgttat acgagtttg gcggcgcat aataagaca catagtggc ataaaaagc cacgcttgt ggggtttgt ggcaaaagg ttgtgtggt tctggttct cttgcaatg catatgaag actcacacg ggcgttaaa gaccacatt tgtccttac tgtggcaaa ggttttacc acaccgagt aatctgacc atacataaa aggacgcac acaggtgag cgtccttac gtttgcaac gaatgtggt aaaggtttt cctgatccg tcgagactc acagtccat gtgcgatca cacagcggc gaaaagcct tacgtttgt agcgtttgt ggccgtggt tgtgtcagt tcgtctcaa ttaaaaaaa cacagaaga atacatact ggcgaaaag ccataccag tgcaatcta tgtccaaag gcatttcca cgaagcgaa gatttacgc attcatata aaaactcac actggggat agaaaccat atttgtcct ttttgcaaa aaaggcttt tatcaggct tcaacttta aaagtacac cttagaata cacaccggg gaaaaaccg tacacttgc aatatttgt aataaagct ttttcgcaa accgggccc ctatccacg cacatgaaa acgcattca tag
- >TC013711 RA
ccaatcatc gaaataacc taaattaat aaacaaacg attttttaa aaatgtctt tcgaaacac tccaaatcg aaaagatat gtagaacct gcctttcgg aagaaggtg acatgcgtt cggtattta gcattgacg aatctatcg gcgaaacaa tgcgtttgt ttgaaatgt taatgtctt gtgctgcag tgcaggttt tggaaaacg acggattgc ccaaccaag tttgtttac aatgtgttc attacataa ctcgtgctt tttcattta aacaattat gcgagagat cagatgcaa cgttgcggc aactcctag gcaaaccca tgcaggcca cttttatgg aattaaaac cattattgt ctagtgatc aacacgaac cttcattta acgatgtta taaataccg tcacggaaa atatacagg ccgtaacag attccgtaa atgaggctt ttagtagcg cagttgcaa cagtgaata gcatagacg ttggggatt ctttcaagt cggaagggt cccaagaca tgaaaatga aactggagt ttgtggaca atgacgtgt taggaattg atccagatt cgtgtggta gcgatgccg aaagcaaaa taaacgaac tgagagcta aaagagaat caaagaaaa agaaaatta gagacgaca ctgaacaac cgatttatc catgtgagg aatgcactc agtgtttca ctacaatga tcgatttga aggcccatg ctaaaacac atccgaaaa ttagcagac acatttgta aaatctgta atcaaggtt tcgcaagtg cgagcactt tgtgtcgac acatgaaag tacatgatg gcgcaaaac ggcatttgt gtagtgagt gtggcaaag gttttgcga gatcggatg atcttacaa gacatataa gaacacaca ctggtgaaa agccttttc cttgtaaat tatgcggca aatcgtttg ctcaatctt ttcgtctgt tggagcata tgagggcgc atgcgaacg aaaaaagtt tcatttgtt cagtttgtg gcaaagcct tttcacgtt atacgagtt tggcggcgc ataataaga cacatagtg gcataaaaa gccacgctt gtggggttt gtggcaaaa ggttgtgtg gttctggtt ctcttgcaa tgcatatga agactcaca cgggcgtta aagaccaca tttgtcctt actgtggca aaggtttta ccacaccga gtaatctga ccatacata aaaggacgc acacaggtg agcgtcctt acgtttgca acgaatgtg gtaaaggtt ttcctgatc cgtcgagac tcacagtcc atgtgcgat cacacagcg gcgaaaagc cttacgttt gtagcgttt gtggccgtg gttgtgtca gttcgtctc aattaaaaa aacacagaa gaatacata ctggcgaaa agccatacc agtgcaatc tatgtccaa aggcatttc cacgaagcg aagatttac gcattcata taaaaactc acactgggg atagaaacc atatttgtc ctttttgca aaaaaggct tttatcagg cttcaactt taaaagtac accttagaa tacacaccg gggaaaaac cgtacactt gcaatattt gtaataaag ctttttcgc aaaccgggc ccctatcca cgcacatga aaacgcatt catagcgta cctaaactt tatttattt atttactaa actttacat ttcagtgag attatattg tataaagta gcgtctatt tagtagtta tacacatag gaacacgta tttaataac ttgggacta tgttggtga aatattatt atgtactgc ctaaataaa tgcaatttt aataa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
