Status
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Gene information
General information
TC013711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5474656..5477008
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013711
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08240

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013711 RA
      MSFETLQIE KICRTCLSE EGDMRSVFS IDESIGETM 
      RLFEMLMSC AAVQVLEND GLPNQVCLQ CVHYITRAF 
      SFKQLCERS DATLRQLLG KPMQATFME LKPLLSSDQ 
      HEPSFNDVI NTVTENIQA VTDSVNEAF SSAVATVNS 
      IDVGDSFKS EGSQDMKMK LEFVDNDVL GIDPDSCGS 
      DAESKINEL RAKRESKKK KIRDDTEQP IYPCEECTQ 
      CFTTMIDLK AHAKTHPKI SRHICKICN QGFASASTL 
      CRHMKVHDG AKRHLCSEC GKGFARSDD LTRHIRTHT 
      GEKPFPCKL CGKSFAQSF RLLEHMRAH ANEKSFICS 
      VCGKAFSRY TSLAAHNKT HSGIKSHAC GVCGKRLCG 
      SGSLAMHMK THTGVKDHI CPYCGKGFT TPSNLTIHK 
      RTHTGERPY VCNECGKGF PDPSRLTVH VRSHSGEKP 
      YVCSVCGRG CVSSSQLKK HRRIHTGEK PYQCNLCPK 
      AFPRSEDLR IHIKTHTGD RNHICPFCK KGFYQASTL 
      KVHLRIHTG EKPYTCNIC NKAFSQTGP LSTHMKTHS
    CDS sequence
    • >TC013711 RA
      atgtctttc gaaacactc caaatcgaa aagatatgt 
      agaacctgc ctttcggaa gaaggtgac atgcgttcg 
      gtatttagc attgacgaa tctatcggc gaaacaatg 
      cgtttgttt gaaatgtta atgtcttgt gctgcagtg 
      caggttttg gaaaacgac ggattgccc aaccaagtt 
      tgtttacaa tgtgttcat tacataact cgtgctttt 
      tcatttaaa caattatgc gagagatca gatgcaacg 
      ttgcggcaa ctcctaggc aaacccatg caggccact 
      tttatggaa ttaaaacca ttattgtct agtgatcaa 
      cacgaacct tcatttaac gatgttata aataccgtc 
      acggaaaat atacaggcc gtaacagat tccgtaaat 
      gaggctttt agtagcgca gttgcaaca gtgaatagc 
      atagacgtt ggggattct ttcaagtcg gaagggtcc 
      caagacatg aaaatgaaa ctggagttt gtggacaat 
      gacgtgtta ggaattgat ccagattcg tgtggtagc 
      gatgccgaa agcaaaata aacgaactg agagctaaa 
      agagaatca aagaaaaag aaaattaga gacgacact 
      gaacaaccg atttatcca tgtgaggaa tgcactcag 
      tgtttcact acaatgatc gatttgaag gcccatgct 
      aaaacacat ccgaaaatt agcagacac atttgtaaa 
      atctgtaat caaggtttc gcaagtgcg agcactttg 
      tgtcgacac atgaaagta catgatggc gcaaaacgg 
      catttgtgt agtgagtgt ggcaaaggt tttgcgaga 
      tcggatgat cttacaaga catataaga acacacact 
      ggtgaaaag ccttttcct tgtaaatta tgcggcaaa 
      tcgtttgct caatctttt cgtctgttg gagcatatg 
      agggcgcat gcgaacgaa aaaagtttc atttgttca 
      gtttgtggc aaagccttt tcacgttat acgagtttg 
      gcggcgcat aataagaca catagtggc ataaaaagc 
      cacgcttgt ggggtttgt ggcaaaagg ttgtgtggt 
      tctggttct cttgcaatg catatgaag actcacacg 
      ggcgttaaa gaccacatt tgtccttac tgtggcaaa 
      ggttttacc acaccgagt aatctgacc atacataaa 
      aggacgcac acaggtgag cgtccttac gtttgcaac 
      gaatgtggt aaaggtttt cctgatccg tcgagactc 
      acagtccat gtgcgatca cacagcggc gaaaagcct 
      tacgtttgt agcgtttgt ggccgtggt tgtgtcagt 
      tcgtctcaa ttaaaaaaa cacagaaga atacatact 
      ggcgaaaag ccataccag tgcaatcta tgtccaaag 
      gcatttcca cgaagcgaa gatttacgc attcatata 
      aaaactcac actggggat agaaaccat atttgtcct 
      ttttgcaaa aaaggcttt tatcaggct tcaacttta 
      aaagtacac cttagaata cacaccggg gaaaaaccg 
      tacacttgc aatatttgt aataaagct ttttcgcaa 
      accgggccc ctatccacg cacatgaaa acgcattca 
      tag
    mRNA sequence
    • >TC013711 RA
      ccaatcatc gaaataacc taaattaat aaacaaacg 
      attttttaa aaatgtctt tcgaaacac tccaaatcg 
      aaaagatat gtagaacct gcctttcgg aagaaggtg 
      acatgcgtt cggtattta gcattgacg aatctatcg 
      gcgaaacaa tgcgtttgt ttgaaatgt taatgtctt 
      gtgctgcag tgcaggttt tggaaaacg acggattgc 
      ccaaccaag tttgtttac aatgtgttc attacataa 
      ctcgtgctt tttcattta aacaattat gcgagagat 
      cagatgcaa cgttgcggc aactcctag gcaaaccca 
      tgcaggcca cttttatgg aattaaaac cattattgt 
      ctagtgatc aacacgaac cttcattta acgatgtta 
      taaataccg tcacggaaa atatacagg ccgtaacag 
      attccgtaa atgaggctt ttagtagcg cagttgcaa 
      cagtgaata gcatagacg ttggggatt ctttcaagt 
      cggaagggt cccaagaca tgaaaatga aactggagt 
      ttgtggaca atgacgtgt taggaattg atccagatt 
      cgtgtggta gcgatgccg aaagcaaaa taaacgaac 
      tgagagcta aaagagaat caaagaaaa agaaaatta 
      gagacgaca ctgaacaac cgatttatc catgtgagg 
      aatgcactc agtgtttca ctacaatga tcgatttga 
      aggcccatg ctaaaacac atccgaaaa ttagcagac 
      acatttgta aaatctgta atcaaggtt tcgcaagtg 
      cgagcactt tgtgtcgac acatgaaag tacatgatg 
      gcgcaaaac ggcatttgt gtagtgagt gtggcaaag 
      gttttgcga gatcggatg atcttacaa gacatataa 
      gaacacaca ctggtgaaa agccttttc cttgtaaat 
      tatgcggca aatcgtttg ctcaatctt ttcgtctgt 
      tggagcata tgagggcgc atgcgaacg aaaaaagtt 
      tcatttgtt cagtttgtg gcaaagcct tttcacgtt 
      atacgagtt tggcggcgc ataataaga cacatagtg 
      gcataaaaa gccacgctt gtggggttt gtggcaaaa 
      ggttgtgtg gttctggtt ctcttgcaa tgcatatga 
      agactcaca cgggcgtta aagaccaca tttgtcctt 
      actgtggca aaggtttta ccacaccga gtaatctga 
      ccatacata aaaggacgc acacaggtg agcgtcctt 
      acgtttgca acgaatgtg gtaaaggtt ttcctgatc 
      cgtcgagac tcacagtcc atgtgcgat cacacagcg 
      gcgaaaagc cttacgttt gtagcgttt gtggccgtg 
      gttgtgtca gttcgtctc aattaaaaa aacacagaa 
      gaatacata ctggcgaaa agccatacc agtgcaatc 
      tatgtccaa aggcatttc cacgaagcg aagatttac 
      gcattcata taaaaactc acactgggg atagaaacc 
      atatttgtc ctttttgca aaaaaggct tttatcagg 
      cttcaactt taaaagtac accttagaa tacacaccg 
      gggaaaaac cgtacactt gcaatattt gtaataaag 
      ctttttcgc aaaccgggc ccctatcca cgcacatga 
      aaacgcatt catagcgta cctaaactt tatttattt 
      atttactaa actttacat ttcagtgag attatattg 
      tataaagta gcgtctatt tagtagtta tacacatag 
      gaacacgta tttaataac ttgggacta tgttggtga 
      aatattatt atgtactgc ctaaataaa tgcaatttt 
      aataa
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