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Gene information
General information
TC013713 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5463690..5466438
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013713
    iBeetle screen iB_08239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013713 RA
      MNRRDYDKD KETIRTFLL EFCIQDDSG QKNFKYSNQ 
      LTKLAHREQ IALYIELDD LYEHDDDLA KAVINNTRR 
      YTTLFSDVV FELLPTFVE REVVAKDAL DVYIEHRLM 
      MEQRLRQPN EQRDARNKF PPELLRRYE IYFKDLSTT 
      KTVPIREVK AEHIGKLVT VRGIVTRST EVKPMMTVA 
      TYTCDQCGA ETYQPVHGL SFMPVLMCP SEDCRVNKS 
      GGRLYLQTR GSKFVKFQE LKIQEHSDQ VPVGHIPRT 
      LTIFCRGEV TRLALPGHH VAVTGVFLP LLKTGFKQI 
      MGGLLSETY VEAHRIALV NKTEEDESS SKPLTPEEL 
      SVLTEDDFY TKLAVSLAP EIYGHLDVK KALLLLLVG 
      GVDRRPDGM KIRGNINIC LMGDPGVAK SQLLGYIDR 
      LAPRSQYTT GRGSSGVGL TASVMKDPM TGEMMLEGG 
      ALVLADQGV CCIDEFDKM ADADRTAIH EVMEQQTIS 
      IAKAGIMTC LNARVSILA AANPAYGRY NPKRTIEQN 
      IQLPAALLS RFDLLWLIQ DKADRDNDL KLAKHITYV 
      HQHCKQPPT QIKSLDMGV MRKYIALCK LKEPVIPED 
      LTEYIVNAY VELRNVARN SRDMTFTSA RNLLGILRL 
      STALARLRL SNVVEKDDV NEAIRLMEM SKDSLNQTF 
      EKGARPPNV NDQIFAIIR DLAGESKTV KLSDVMNKC 
      ASKGHTPLE VDSCIEEYE ELNVWQVNL TRTKITFI
    CDS sequence
    • >TC013713 RA
      atgaaccga cgtgattac gacaaagat aaagagacc 
      ataagaacc ttcttgtta gagttttgc attcaagat 
      gattccggt cagaaaaac tttaaatat tcgaatcag 
      ttgacaaaa ctggctcat cgcgagcaa atagccctt 
      tacatcgag cttgacgat ctttacgaa cacgacgat 
      gatttagcc aaagctgta ataaacaac actcgtcgg 
      tacacgact ttattctcc gacgttgtc ttcgaactg 
      ttgcccaca ttcgtggaa cgcgaagtc gtggccaaa 
      gacgctttg gacgtttac attgaacac aggctaatg 
      atggagcag cgtttgaga caaccgaac gagcagaga 
      gatgctcgt aataaattt ccaccggaa ttattacgc 
      agatacgaa atctacttc aaagactta tcaactact 
      aaaaccgtc ccaattcgc gaagtcaaa gccgaacac 
      attgggaaa ctggtcacc gttaggggc atagtgacg 
      cgaagcacc gaagtcaaa cctatgatg accgtagcg 
      acctacact tgcgaccaa tgcggggct gagacctac 
      caacctgtc cacggacta agtttcatg ccggtgttg 
      atgtgtcct agtgaagac tgccgtgtg aacaaatca 
      ggggggcgc ctatacctc caaacccgt ggctccaaa 
      ttcgtcaaa ttccaggag ctcaaaatc caggaacac 
      agtgaccaa gtcccagtg gggcacatc ccccggact 
      ttgactatt ttttgtcgg ggcgaagtc actcgcctg 
      gcccttccg gggcaccac gtggccgtc acaggcgtg 
      ttcctccct ttgctcaag accggcttc aaacaaatc 
      atggggggg ctcctgtcg gagacttac gtcgaggcg 
      cacagaatc gctttggtg aacaaaacc gaagaggat 
      gaaagctcg tccaaaccc ctcacacct gaagaacta 
      tcggtcttg accgaagac gatttttac acaaaattg 
      gcggtgtct ctagcgccg gaaatctac ggtcatctt 
      gacgttaag aaggctttg cttcttttg ctggttggg 
      ggagtggac cgcagacct gacggtatg aagatccgg 
      gggaatatc aatatttgc ctgatgggg gaccccgga 
      gtcgccaag tcgcaactt ttggggtac attgatcgg 
      ttagcgcca cgaagtcag tataccaca ggacggggc 
      tcttcggga gtggggctc actgcctcg gttatgaag 
      gaccccatg acgggggag atgatgctg gaagggggc 
      gcactggtc ttggccgat cagggggtt tgttgtatt 
      gacgaattc gataaaatg gcggatgcg gacaggacg 
      gccattcac gaagttatg gaacaacaa acaatcagc 
      attgctaag gcgggtatt atgacttgt ttgaacgcc 
      agagtctcg attttggcg gcggctaac ccggcctat 
      ggccggtac aacccgaag cggacaatc gagcaaaat 
      atccaacta ccggcggcc cttttatcc cgtttcgat 
      ctcctttgg ttgattcag gataaagcg gatcgcgat 
      aatgatctg aaactcgcc aaacacatc acatacgtg 
      caccagcac tgcaaacag cccccgact caaatcaaa 
      tcgcttgat atgggcgtt atgcggaaa tacatcgca 
      ctgtgtaag ttgaaagaa ccggtgatt cccgaagat 
      ttgactgaa tacatcgtt aatgcttat gtggagttg 
      agaaatgtg gcccggaat tctagagac atgactttt 
      acttcggct aggaatttg ttggggatt ttgaggctg 
      tcgacggct ttagccagg ctgagattg tccaatgtt 
      gtggagaaa gacgatgtt aatgaagct ataaggttg 
      atggaaatg agcaaagat tctcttaac cagactttc 
      gaaaaaggg gcaagacca cccaatgtc aacgaccaa 
      atctttgcc attattaga gacttggct ggagaatcg 
      aaaacagtc aaactatca gacgttatg aacaaatgt 
      gccagtaag ggacacact cctctggaa gttgattcc 
      tgtatcgaa gagtatgag gaattgaac gtgtggcaa 
      gtgaatctg actcgcacc aaaatcaca tttatttaa
    mRNA sequence
    • >TC013713 RA
      aaactggcg tgtgttgtg tgcaaacag gtggtcgta 
      aataaacgt tagtaaaac tccaattcc ggcttgaag 
      tagtgtaaa ttcgccaaa aatcatgaa ccgacgtga 
      ttacgacaa agataaaga gaccataag aaccttctt 
      gttagagtt ttgcattca agatgattc cggtcagaa 
      aaactttaa atattcgaa tcagttgac aaaactggc 
      tcatcgcga gcaaatagc cctttacat cgagcttga 
      cgatcttta cgaacacga cgatgattt agccaaagc 
      tgtaataaa caacactcg tcggtacac gactttatt 
      ctccgacgt tgtcttcga actgttgcc cacattcgt 
      ggaacgcga agtcgtggc caaagacgc tttggacgt 
      ttacattga acacaggct aatgatgga gcagcgttt 
      gagacaacc gaacgagca gagagatgc tcgtaataa 
      atttccacc ggaattatt acgcagata cgaaatcta 
      cttcaaaga cttatcaac tactaaaac cgtcccaat 
      tcgcgaagt caaagccga acacattgg gaaactggt 
      caccgttag gggcatagt gacgcgaag caccgaagt 
      caaacctat gatgaccgt agcgaccta cacttgcga 
      ccaatgcgg ggctgagac ctaccaacc tgtccacgg 
      actaagttt catgccggt gttgatgtg tcctagtga 
      agactgccg tgtgaacaa atcaggggg gcgcctata 
      cctccaaac ccgtggctc caaattcgt caaattcca 
      ggagctcaa aatccagga acacagtga ccaagtccc 
      agtggggca catcccccg gactttgac tattttttg 
      tcggggcga agtcactcg cctggccct tccggggca 
      ccacgtggc cgtcacagg cgtgttcct ccctttgct 
      caagaccgg cttcaaaca aatcatggg ggggctcct 
      gtcggagac ttacgtcga ggcgcacag aatcgcttt 
      ggtgaacaa aaccgaaga ggatgaaag ctcgtccaa 
      acccctcac acctgaaga actatcggt cttgaccga 
      agacgattt ttacacaaa attggcggt gtctctagc 
      gccggaaat ctacggtca tcttgacgt taagaaggc 
      tttgcttct tttgctggt tgggggagt ggaccgcag 
      acctgacgg tatgaagat ccgggggaa tatcaatat 
      ttgcctgat gggggaccc cggagtcgc caagtcgca 
      acttttggg gtacattga tcggttagc gccacgaag 
      tcagtatac cacaggacg gggctcttc gggagtggg 
      gctcactgc ctcggttat gaaggaccc catgacggg 
      ggagatgat gctggaagg gggcgcact ggtcttggc 
      cgatcaggg ggtttgttg tattgacga attcgataa 
      aatggcgga tgcggacag gacggccat tcacgaagt 
      tatggaaca acaaacaat cagcattgc taaggcggg 
      tattatgac ttgtttgaa cgccagagt ctcgatttt 
      ggcggcggc taacccggc ctatggccg gtacaaccc 
      gaagcggac aatcgagca aaatatcca actaccggc 
      ggccctttt atcccgttt cgatctcct ttggttgat 
      tcaggataa agcggatcg cgataatga tctgaaact 
      cgccaaaca catcacata cgtgcacca gcactgcaa 
      acagccccc gactcaaat caaatcgct tgatatggg 
      cgttatgcg gaaatacat cgcactgtg taagttgaa 
      agaaccggt gattcccga agatttgac tgaatacat 
      cgttaatgc ttatgtgga gttgagaaa tgtggcccg 
      gaattctag agacatgac ttttacttc ggctaggaa 
      tttgttggg gattttgag gctgtcgac ggctttagc 
      caggctgag attgtccaa tgttgtgga gaaagacga 
      tgttaatga agctataag gttgatgga aatgagcaa 
      agattctct taaccagac tttcgaaaa aggggcaag 
      accacccaa tgtcaacga ccaaatctt tgccattat 
      tagagactt ggctggaga atcgaaaac agtcaaact 
      atcagacgt tatgaacaa atgtgccag taagggaca 
      cactcctct ggaagttga ttcctgtat cgaagagta 
      tgaggaatt gaacgtgtg gcaagtgaa tctgactcg 
      caccaaaat cacatttat ttaagattt agttgtttg 
      taccacttt tataacttg tatttttta tatttcaat 
      aaagcatga atccaatt
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