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Gene information
General information
TC031140
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013744 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4896784..4924891
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031140
    iBeetle screen iB_05487

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    iBeetle screen iB_08234

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna sometimesnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    mandible sometimesnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    labium position posteriorized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labium potentiallytransformed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages sometimes fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    prothorax segment duplicated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    metathorax sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    prothorax potentially transformed - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment mostlynumber decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031140 RA
      MEKFQEQQE PDDQDQVYE VEEPETSST SKIIISNLG 
PNVRFEDIE HLLLQHGAV VACDKLTSK DPTTQTVQI 
TFESTEQAQ QAVNQLNGI EIDSRPIKV ELAVEKRGR 
RGPRGPGAP FGGLPGQSR QTDFPLRIL VQSDMVGAI 
IGRQGSTIR QITQQTRAR VDVHRKDNV GSLEKAITI 
YGNPENCTN ACKRILEVM QQEANNTNK GEICLKILA 
HNNLIGRII GKGGNTIKR IMQETDTKI TVSSINDIN 
SFNLERIIT VKGAIDNMS RAEAQISAK LRQSYENDL 
QAMAPQTMM FPGLHPMAM MATAGQAPY PGMYPAGAA 
QGGGDSQET TYLYIPNNA VGAIIGTKG SHIRNIIRF 
SGASVKIAP IDETKPQET QNERRVTIV GSPEAQWKA 
QYLIFEKMR EEGFVAGSD DVRLTVEIM VPSSQVGRI 
IGKGGQNVR ELQRVTGSV IKLPEQGAS PQEDETTVH 
IIGPFFSVQ SAQRRIRAM VLQSVVPLQ GRPRQPRQP 
KDPSQSEAA AALDPQQQQ QQTPPQQQQ QQQQQPPQQ 
Q
    • >TC031140 RB
      MEKFQEQQE PDDQDQVYE VEEPETSST SKIIISNLG 
PNVRFEDIE HLLLQHGAV VACDKLTSK DPTTQTVQI 
TFESTEQAQ QAVNQLNGI EIDSRPIKV ELAVEKRGR 
RGPRGPGAP FGGLPGQSR QTDFPLRIL VQSDMVGAI 
IGRQGSTIR QITQQTRAR VDVHRKDNV GSLEKAITI 
YGNPENCTN ACKRILEVM QQEANNTNK GEICLKILA 
HNNLIGRII GKGGNTIKR IMQETDTKI TVSSINDIN 
SFNLERIIT VKGAIDNMS RAEAQISAK LRQSYENDL 
QAMAPQTMM FPGLHPMAM MATAGIGYG SRGLYTGQA 
PYPGMYPAG AAQGGGDSQ ETTYLYIPN NAVGAIIGT 
KGSHIRNII RFSGASVKI APIDETKPQ ETQNERRVT 
IVGSPEAQW KAQYLIFEK MREEGFVAG SDDVRLTVE 
IMVPSSQVG RIIGKGGQN VRELQRVTG SVIKLPEQG 
ASPQEDETT VHIIGPFFS VQSAQRRIR AMVLQSVVP 
LQGRPRQPR QPKDPSQSE AAAALDPQQ QQQQTPPQQ 
QQQQQQQPP QQQ
    CDS sequence
    • >TC031140 RA
      atggaaaag ttccaggaa cagcaggaa cctgacgat 
caggatcag gtttacgaa gtcgaggaa cctgaaact 
agttcaaca tcaaaaatt attatatcc aatttgggt 
ccaaacgtc cggttcgaa gacatcgaa catctactt 
ttacaacac ggagctgtc gttgcatgt gataaactt 
acctcaaaa gatcccacc acacaaacg gtgcaaatt 
actttcgaa agcacggaa caagctcaa caggccgta 
aatcaactt aatggtata gaaatagat agtagaccc 
atcaaagtg gaattggct gtggaaaaa cggggccgt 
aggggacct cgagggcca ggcgcccct ttcgggggc 
ctgcctggt cagagtaga caaacggat tttccatta 
agaatactt gtacaaagc gatatggtt ggagctatt 
atagggaga cagggatcg acgatacga cagatcact 
caacagacg agagcgaga gtcgacgtt catagaaaa 
gacaatgta gggtcgtta gaaaaagcc atcactatt 
tacggaaat ccggaaaat tgcacaaac gcctgcaaa 
cgaatttta gaagttatg cagcaagag gccaacaac 
acgaataaa ggcgaaata tgtttgaaa attctggcg 
cacaacaac ctcatcgga cgtatcata ggcaagggg 
ggaaacacg atcaaaagg ataatgcaa gagacggac 
acgaagatc accgtctcg agcataaac gacataaac 
tcgttcaat ttggagcga atcatcacc gtgaaagga 
gccatcgac aatatgagc cgagcggag gcccaaatc 
agcgccaaa ttacggcag agctacgaa aacgatctc 
caggcgatg gcgccgcaa acgatgatg ttccccgga 
ctgcatccg atggcgatg atggccacc gcaggtcaa 
gccccctat cccggaatg tatcccgca ggtgcggcc 
cagggcgga ggcgacagc caggaaacg acctatctg 
tacataccc aacaacgcc gtcggagcc atcatcgga 
acgaaaggc tcgcatatt cgaaatatc attcgattc 
tcgggagct tccgtcaag atcgcgccc atcgacgag 
acgaaaccg caagaaacg caaaacgaa agaagagtc 
acgattgtc ggctcaccc gaagcgcag tggaaggcg 
cagtatttg atttttgag aagatgcga gaggaaggt 
ttcgtcgct ggatcggat gatgtacga ttaacggtg 
gaaataatg gttcctagc tcgcaagtc ggccgaatc 
atcggcaaa ggaggtcaa aatgttcgc gaactacaa 
cgcgtaacc ggaagcgta attaaactg ccagaacag 
ggtgcttct cctcaagag gatgaaaca acggtgcat 
atcattgga ccatttttc agtgttcaa tcggcgcaa 
cgtcgcata cgcgccatg gtattgcaa tcagtagtg 
cctcttcag ggaagaccg cgtcaaccg cgccaacca 
aaagacccc agccaatcg gaagccgcc gctgcgttg 
gacccacaa cagcagcaa cagcaaacg ccaccgcag 
cagcagcaa caacaacag cagcaacca ccacaacaa 
cagtaa
    • >TC031140 RB
      atggaaaag ttccaggaa cagcaggaa cctgacgat 
caggatcag gtttacgaa gtcgaggaa cctgaaact 
agttcaaca tcaaaaatt attatatcc aatttgggt 
ccaaacgtc cggttcgaa gacatcgaa catctactt 
ttacaacac ggagctgtc gttgcatgt gataaactt 
acctcaaaa gatcccacc acacaaacg gtgcaaatt 
actttcgaa agcacggaa caagctcaa caggccgta 
aatcaactt aatggtata gaaatagat agtagaccc 
atcaaagtg gaattggct gtggaaaaa cggggccgt 
aggggacct cgagggcca ggcgcccct ttcgggggc 
ctgcctggt cagagtaga caaacggat tttccatta 
agaatactt gtacaaagc gatatggtt ggagctatt 
atagggaga cagggatcg acgatacga cagatcact 
caacagacg agagcgaga gtcgacgtt catagaaaa 
gacaatgta gggtcgtta gaaaaagcc atcactatt 
tacggaaat ccggaaaat tgcacaaac gcctgcaaa 
cgaatttta gaagttatg cagcaagag gccaacaac 
acgaataaa ggcgaaata tgtttgaaa attctggcg 
cacaacaac ctcatcgga cgtatcata ggcaagggg 
ggaaacacg atcaaaagg ataatgcaa gagacggac 
acgaagatc accgtctcg agcataaac gacataaac 
tcgttcaat ttggagcga atcatcacc gtgaaagga 
gccatcgac aatatgagc cgagcggag gcccaaatc 
agcgccaaa ttacggcag agctacgaa aacgatctc 
caggcgatg gcgccgcaa acgatgatg ttccccgga 
ctgcatccg atggcgatg atggccacc gcaggaatt 
ggttatggt tcaagaggt ctgtacaca ggtcaagcc 
ccctatccc ggaatgtat cccgcaggt gcggcccag 
ggcggaggc gacagccag gaaacgacc tatctgtac 
atacccaac aacgccgtc ggagccatc atcggaacg 
aaaggctcg catattcga aatatcatt cgattctcg 
ggagcttcc gtcaagatc gcgcccatc gacgagacg 
aaaccgcaa gaaacgcaa aacgaaaga agagtcacg 
attgtcggc tcacccgaa gcgcagtgg aaggcgcag 
tatttgatt tttgagaag atgcgagag gaaggtttc 
gtcgctgga tcggatgat gtacgatta acggtggaa 
ataatggtt cctagctcg caagtcggc cgaatcatc 
ggcaaagga ggtcaaaat gttcgcgaa ctacaacgc 
gtaaccgga agcgtaatt aaactgcca gaacagggt 
gcttctcct caagaggat gaaacaacg gtgcatatc 
attggacca tttttcagt gttcaatcg gcgcaacgt 
cgcatacgc gccatggta ttgcaatca gtagtgcct 
cttcaggga agaccgcgt caaccgcgc caaccaaaa 
gaccccagc caatcggaa gccgccgct gcgttggac 
ccacaacag cagcaacag caaacgcca ccgcagcag 
cagcaacaa caacagcag caaccacca caacaacag 
taa
    mRNA sequence
    • >TC031140 RA
      ttagtacgc atttagcac gtatttaga gtgatatgt 
tgtgttacg tcatttttc cgaaaagga ctttttggt 
ttcgctttc aaaagtgac cctttcggc aacatttgg 
attttcgtc tcaaataac aacacatcc ggtagtcca 
tttacgtct gttttgttt tgtttgaat ttttgattt 
tgatgctgc ttttggtcc ggtggtgat attgtggct 
atgttgtga cggttttgt gatttctct attttcctg 
ttggatttt tgtttttgt gtttttacg aacatcaag 
tttcggtta ttgctcaaa atttcgagt cgatgtctg 
tttcgttgg tttaatgtt gagtttctt cggaggttt 
tggcggaca gataaagtt cgttttcga caaagactg 
attaggctt aagtagtgt gtagtgcgt gtgtggttt 
agtggtttg ttgccttgt ccatttttg gatactagt 
gatctaagt acacatttt caacatgga aaagttcca 
ggaacagca ggaacctga cgatcagga tcaggttta 
cgaagtcga ggaacctga aactagttc aacatcaaa 
aattattat atccaattt gggtccaaa cgtccggtt 
cgaagacat cgaacatct acttttaca acacggagc 
tgtcgttgc atgtgataa acttacctc aaaagatcc 
caccacaca aacggtgca aattacttt cgaaagcac 
ggaacaagc tcaacaggc cgtaaatca acttaatgg 
tatagaaat agatagtag acccatcaa agtggaatt 
ggctgtgga aaaacgggg ccgtagggg acctcgagg 
gccaggcgc ccctttcgg gggcctgcc tggtcagag 
tagacaaac ggattttcc attaagaat acttgtaca 
aagcgatat ggttggagc tattatagg gagacaggg 
atcgacgat acgacagat cactcaaca gacgagagc 
gagagtcga cgttcatag aaaagacaa tgtagggtc 
gttagaaaa agccatcac tatttacgg aaatccgga 
aaattgcac aaacgcctg caaacgaat tttagaagt 
tatgcagca agaggccaa caacacgaa taaaggcga 
aatatgttt gaaaattct ggcgcacaa caacctcat 
cggacgtat cataggcaa ggggggaaa cacgatcaa 
aaggataat gcaagagac ggacacgaa gatcaccgt 
ctcgagcat aaacgacat aaactcgtt caatttgga 
gcgaatcat caccgtgaa aggagccat cgacaatat 
gagccgagc ggaggccca aatcagcgc caaattacg 
gcagagcta cgaaaacga tctccaggc gatggcgcc 
gcaaacgat gatgttccc cggactgca tccgatggc 
gatgatggc caccgcagg tcaagcccc ctatcccgg 
aatgtatcc cgcaggtgc ggcccaggg cggaggcga 
cagccagga aacgaccta tctgtacat acccaacaa 
cgccgtcgg agccatcat cggaacgaa aggctcgca 
tattcgaaa tatcattcg attctcggg agcttccgt 
caagatcgc gcccatcga cgagacgaa accgcaaga 
aacgcaaaa cgaaagaag agtcacgat tgtcggctc 
acccgaagc gcagtggaa ggcgcagta tttgatttt 
tgagaagat gcgagagga aggtttcgt cgctggatc 
ggatgatgt acgattaac ggtggaaat aatggttcc 
tagctcgca agtcggccg aatcatcgg caaaggagg 
tcaaaatgt tcgcgaact acaacgcgt aaccggaag 
cgtaattaa actgccaga acagggtgc ttctcctca 
agaggatga aacaacggt gcatatcat tggaccatt 
tttcagtgt tcaatcggc gcaacgtcg catacgcgc 
catggtatt gcaatcagt agtgcctct tcagggaag 
accgcgtca accgcgcca accaaaaga ccccagcca 
atcggaagc cgccgctgc gttggaccc acaacagca 
gcaacagca aacgccacc gcagcagca gcaacaaca 
acagcagca accaccaca acaacagta acgccagct 
agcaacatt cactgccct gtgcatggg tttatgcat 
accctattg tacaattaa catctcgag gccttccac 
taatgcaat atcatcctt ccagtctta gtgtcgtcg 
tagcgcatt gttgtcgcc gaacacaac caaatatta 
gctgtgcgc gcgcgtgtt ttaactcta ttaaattaa 
gtaaaacaa caacaagaa gaagaagaa atgtgtgaa 
ccgcgaata cctcagcaa gtgctttat tgccgatac 
gtccatttt gttcagttt gatgatttt ccgctacac 
tggagttta tttaagtta ttggttcta gtggaaagt 
ttggagaag aaacaggaa tatggtatg ataaactgt 
gtgcccaac gttacaatc tacctcaac taatcaaaa 
taaagttac aagttatag ccatagtaa atttccgtt 
acgagatac agcaaccaa ccaaccatt caaacctca 
ctcactcac aaccagcag tgttttctt ttgttgtac 
acgatctac ctgatgaaa ggactacca aagacaagg 
cgttgttac aaaccattg tttatcgct tcactggat 
tttgaccgc tcgcaccta aacaaacta tcgacgaat 
tcagccaaa acatttcga aagacagga ttttcaacc 
gagcaagca agagcagtc accgttatc catctgaag 
agtcccact taaactagc actacatca aaacaaggc 
gaaagttcc tctgcagag tccggtggc agaggatgc 
gagacggcc atcacaacg gacaggagt cacaactct 
tgcatcctc tgcgctgtt aacgtggct gttgttaat 
tattaagtg tgaatgaag agctttaat aagaaaatt 
ttgataata ttaatgcga aattgttgt gcgtgtctt 
tattattat tattgtgtt tgaatttga agagacgtg 
gcaacgatc tttcaatta attactatt taaaatgga 
aactgttaa tcaaagtgt gcgtgtgga aaaagacat 
ttttaactc tattgtaat attattgtt aggagattg 
ttaaaataa tgtttgata aagatgcgg cacgctctc 
caattatta ttatttgtt taacggagt gccgaggcc 
gtgtcgcat ctttgattt ttcttcgtg attgattgt 
tgctgaatt attatgata ccagatagg acagctgtt 
gcggccttg ctgccttgc gtcaaggtc ggccgcgcc 
cctgatagt acaaactcg ggaggtata gattgggcc 
taagagccc ccggtttag tccggactt gtaaaaaat 
atttttgat aaaaattgt ttgttgggg aggatattt 
tttgttaga ttttttaaa aaataatta tagcggggt 
cgctatttt aaaaaattg tgaaaaaat ggatatatt 
a
    • >TC031140 RB
      ttagtacgc atttagcac gtatttaga gtgatatgt 
tgtgttacg tcatttttc cgaaaagga ctttttggt 
ttcgctttc aaaagtgac cctttcggc aacatttgg 
attttcgtc tcaaataac aacacatcc ggtagtcca 
tttacgtct gttttgttt tgtttgaat ttttgattt 
tgatgctgc ttttggtcc ggtggtgat attgtggct 
atgttgtga cggttttgt gatttctct attttcctg 
ttggatttt tgtttttgt gtttttacg aacatcaag 
tttcggtta ttgctcaaa atttcgagt cgatgtctg 
tttcgttgg tttaatgtt gagtttctt cggaggttt 
tggcggaca gataaagtt cgttttcga caaagactg 
attaggctt aagtagtgt gtagtgcgt gtgtggttt 
agtggtttg ttgccttgt ccatttttg gatactagt 
gatctaagt acacatttt caacatgga aaagttcca 
ggaacagca ggaacctga cgatcagga tcaggttta 
cgaagtcga ggaacctga aactagttc aacatcaaa 
aattattat atccaattt gggtccaaa cgtccggtt 
cgaagacat cgaacatct acttttaca acacggagc 
tgtcgttgc atgtgataa acttacctc aaaagatcc 
caccacaca aacggtgca aattacttt cgaaagcac 
ggaacaagc tcaacaggc cgtaaatca acttaatgg 
tatagaaat agatagtag acccatcaa agtggaatt 
ggctgtgga aaaacgggg ccgtagggg acctcgagg 
gccaggcgc ccctttcgg gggcctgcc tggtcagag 
tagacaaac ggattttcc attaagaat acttgtaca 
aagcgatat ggttggagc tattatagg gagacaggg 
atcgacgat acgacagat cactcaaca gacgagagc 
gagagtcga cgttcatag aaaagacaa tgtagggtc 
gttagaaaa agccatcac tatttacgg aaatccgga 
aaattgcac aaacgcctg caaacgaat tttagaagt 
tatgcagca agaggccaa caacacgaa taaaggcga 
aatatgttt gaaaattct ggcgcacaa caacctcat 
cggacgtat cataggcaa ggggggaaa cacgatcaa 
aaggataat gcaagagac ggacacgaa gatcaccgt 
ctcgagcat aaacgacat aaactcgtt caatttgga 
gcgaatcat caccgtgaa aggagccat cgacaatat 
gagccgagc ggaggccca aatcagcgc caaattacg 
gcagagcta cgaaaacga tctccaggc gatggcgcc 
gcaaacgat gatgttccc cggactgca tccgatggc 
gatgatggc caccgcagg aattggtta tggttcaag 
aggtctgta cacaggtca agcccccta tcccggaat 
gtatcccgc aggtgcggc ccagggcgg aggcgacag 
ccaggaaac gacctatct gtacatacc caacaacgc 
cgtcggagc catcatcgg aacgaaagg ctcgcatat 
tcgaaatat cattcgatt ctcgggagc ttccgtcaa 
gatcgcgcc catcgacga gacgaaacc gcaagaaac 
gcaaaacga aagaagagt cacgattgt cggctcacc 
cgaagcgca gtggaaggc gcagtattt gatttttga 
gaagatgcg agaggaagg tttcgtcgc tggatcgga 
tgatgtacg attaacggt ggaaataat ggttcctag 
ctcgcaagt cggccgaat catcggcaa aggaggtca 
aaatgttcg cgaactaca acgcgtaac cggaagcgt 
aattaaact gccagaaca gggtgcttc tcctcaaga 
ggatgaaac aacggtgca tatcattgg accattttt 
cagtgttca atcggcgca acgtcgcat acgcgccat 
ggtattgca atcagtagt gcctcttca gggaagacc 
gcgtcaacc gcgccaacc aaaagaccc cagccaatc 
ggaagccgc cgctgcgtt ggacccaca acagcagca 
acagcaaac gccaccgca gcagcagca acaacaaca 
gcagcaacc accacaaca acagtaacg ccagctagc 
aacattcac tgccctgtg catgggttt atgcatacc 
ctattgtac aattaacat ctcgaggcc ttccactaa 
tgcaatatc atccttcca gtcttagtg tcgtcgtag 
cgcattgtt gtcgccgaa cacaaccaa atattagct 
gtgcgcgcg cgtgtttta actctatta aattaagta 
aaacaacaa caagaagaa gaagaaatg tgtgaaccg 
cgaatacct cagcaagtg ctttattgc cgatacgtc 
cattttgtt cagtttgat gattttccg ctacactgg 
agtttattt aagttattg gttctagtg gaaagtttg 
gagaagaaa caggaatat ggtatgata aactgtgtg 
cccaacgtt acaatctac ctcaactaa tcaaaataa 
agttacaag ttatagcca tagtaaatt tccgttacg 
agatacagc aaccaacca accattcaa acctcactc 
actcacaac cagcagtgt tttcttttg ttgtacacg 
atctacctg atgaaagga ctaccaaag acaaggcgt 
tgttacaaa ccattgttt atcgcttca ctggatttt 
gaccgctcg cacctaaac aaactatcg acgaattca 
gccaaaaca tttcgaaag acaggattt tcaaccgag 
caagcaaga gcagtcacc gttatccat ctgaagagt 
cccacttaa actagcact acatcaaaa caaggcgaa 
agttcctct gcagagtcc ggtggcaga ggatgcgag 
acggccatc acaacggac aggagtcac aactcttgc 
atcctctgc gctgttaac gtggctgtt gttaattat 
taagtgtga atgaagagc tttaataag aaaattttg 
ataatatta atgcgaaat tgttgtgcg tgtctttat 
tattattat tgtgtttga atttgaaga gacgtggca 
acgatcttt caattaatt actatttaa aatggaaac 
tgttaatca aagtgtgcg tgtggaaaa agacatttt 
taactctat tgtaatatt attgttagg agattgtta 
aaataatgt ttgataaag atgcggcac gctctccaa 
ttattatta tttgtttaa cggagtgcc gaggccgtg 
tcgcatctt tgatttttc ttcgtgatt gattgttgc 
tgaattatt atgatacca gataggaca gctgttgcg 
gccttgctg ccttgcgtc aaggtcggc cgcgcccct 
gatagtaca aactcggga ggtatagat tgggcctaa 
gagcccccg gtttagtcc ggacttgta aaaaatatt 
tttgataaa aattgtttg ttggggagg atatttttt 
gttagattt tttaaaaaa taattatag cggggtcgc 
tattttaaa aaattgtga aaaaatgga tatatta
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