Status
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Gene information
General information
TC031140
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013744
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4896784..4924891
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031140
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05487

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    iBeetle screen iB_08234

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna sometimesnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    mandible sometimesnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    labium position posteriorized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labium potentiallytransformed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages sometimes fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    prothorax segment duplicated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    metathorax sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    prothorax potentially transformed - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment mostlynumber decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031140 RA
      MEKFQEQQE PDDQDQVYE VEEPETSST SKIIISNLG 
      PNVRFEDIE HLLLQHGAV VACDKLTSK DPTTQTVQI 
      TFESTEQAQ QAVNQLNGI EIDSRPIKV ELAVEKRGR 
      RGPRGPGAP FGGLPGQSR QTDFPLRIL VQSDMVGAI 
      IGRQGSTIR QITQQTRAR VDVHRKDNV GSLEKAITI 
      YGNPENCTN ACKRILEVM QQEANNTNK GEICLKILA 
      HNNLIGRII GKGGNTIKR IMQETDTKI TVSSINDIN 
      SFNLERIIT VKGAIDNMS RAEAQISAK LRQSYENDL 
      QAMAPQTMM FPGLHPMAM MATAGQAPY PGMYPAGAA 
      QGGGDSQET TYLYIPNNA VGAIIGTKG SHIRNIIRF 
      SGASVKIAP IDETKPQET QNERRVTIV GSPEAQWKA 
      QYLIFEKMR EEGFVAGSD DVRLTVEIM VPSSQVGRI 
      IGKGGQNVR ELQRVTGSV IKLPEQGAS PQEDETTVH 
      IIGPFFSVQ SAQRRIRAM VLQSVVPLQ GRPRQPRQP 
      KDPSQSEAA AALDPQQQQ QQTPPQQQQ QQQQQPPQQ 
      Q
    • >TC031140 RB
      MEKFQEQQE PDDQDQVYE VEEPETSST SKIIISNLG 
      PNVRFEDIE HLLLQHGAV VACDKLTSK DPTTQTVQI 
      TFESTEQAQ QAVNQLNGI EIDSRPIKV ELAVEKRGR 
      RGPRGPGAP FGGLPGQSR QTDFPLRIL VQSDMVGAI 
      IGRQGSTIR QITQQTRAR VDVHRKDNV GSLEKAITI 
      YGNPENCTN ACKRILEVM QQEANNTNK GEICLKILA 
      HNNLIGRII GKGGNTIKR IMQETDTKI TVSSINDIN 
      SFNLERIIT VKGAIDNMS RAEAQISAK LRQSYENDL 
      QAMAPQTMM FPGLHPMAM MATAGIGYG SRGLYTGQA 
      PYPGMYPAG AAQGGGDSQ ETTYLYIPN NAVGAIIGT 
      KGSHIRNII RFSGASVKI APIDETKPQ ETQNERRVT 
      IVGSPEAQW KAQYLIFEK MREEGFVAG SDDVRLTVE 
      IMVPSSQVG RIIGKGGQN VRELQRVTG SVIKLPEQG 
      ASPQEDETT VHIIGPFFS VQSAQRRIR AMVLQSVVP 
      LQGRPRQPR QPKDPSQSE AAAALDPQQ QQQQTPPQQ 
      QQQQQQQPP QQQ
    CDS sequence
    • >TC031140 RA
      atggaaaag ttccaggaa cagcaggaa cctgacgat 
      caggatcag gtttacgaa gtcgaggaa cctgaaact 
      agttcaaca tcaaaaatt attatatcc aatttgggt 
      ccaaacgtc cggttcgaa gacatcgaa catctactt 
      ttacaacac ggagctgtc gttgcatgt gataaactt 
      acctcaaaa gatcccacc acacaaacg gtgcaaatt 
      actttcgaa agcacggaa caagctcaa caggccgta 
      aatcaactt aatggtata gaaatagat agtagaccc 
      atcaaagtg gaattggct gtggaaaaa cggggccgt 
      aggggacct cgagggcca ggcgcccct ttcgggggc 
      ctgcctggt cagagtaga caaacggat tttccatta 
      agaatactt gtacaaagc gatatggtt ggagctatt 
      atagggaga cagggatcg acgatacga cagatcact 
      caacagacg agagcgaga gtcgacgtt catagaaaa 
      gacaatgta gggtcgtta gaaaaagcc atcactatt 
      tacggaaat ccggaaaat tgcacaaac gcctgcaaa 
      cgaatttta gaagttatg cagcaagag gccaacaac 
      acgaataaa ggcgaaata tgtttgaaa attctggcg 
      cacaacaac ctcatcgga cgtatcata ggcaagggg 
      ggaaacacg atcaaaagg ataatgcaa gagacggac 
      acgaagatc accgtctcg agcataaac gacataaac 
      tcgttcaat ttggagcga atcatcacc gtgaaagga 
      gccatcgac aatatgagc cgagcggag gcccaaatc 
      agcgccaaa ttacggcag agctacgaa aacgatctc 
      caggcgatg gcgccgcaa acgatgatg ttccccgga 
      ctgcatccg atggcgatg atggccacc gcaggtcaa 
      gccccctat cccggaatg tatcccgca ggtgcggcc 
      cagggcgga ggcgacagc caggaaacg acctatctg 
      tacataccc aacaacgcc gtcggagcc atcatcgga 
      acgaaaggc tcgcatatt cgaaatatc attcgattc 
      tcgggagct tccgtcaag atcgcgccc atcgacgag 
      acgaaaccg caagaaacg caaaacgaa agaagagtc 
      acgattgtc ggctcaccc gaagcgcag tggaaggcg 
      cagtatttg atttttgag aagatgcga gaggaaggt 
      ttcgtcgct ggatcggat gatgtacga ttaacggtg 
      gaaataatg gttcctagc tcgcaagtc ggccgaatc 
      atcggcaaa ggaggtcaa aatgttcgc gaactacaa 
      cgcgtaacc ggaagcgta attaaactg ccagaacag 
      ggtgcttct cctcaagag gatgaaaca acggtgcat 
      atcattgga ccatttttc agtgttcaa tcggcgcaa 
      cgtcgcata cgcgccatg gtattgcaa tcagtagtg 
      cctcttcag ggaagaccg cgtcaaccg cgccaacca 
      aaagacccc agccaatcg gaagccgcc gctgcgttg 
      gacccacaa cagcagcaa cagcaaacg ccaccgcag 
      cagcagcaa caacaacag cagcaacca ccacaacaa 
      cagtaa
    • >TC031140 RB
      atggaaaag ttccaggaa cagcaggaa cctgacgat 
      caggatcag gtttacgaa gtcgaggaa cctgaaact 
      agttcaaca tcaaaaatt attatatcc aatttgggt 
      ccaaacgtc cggttcgaa gacatcgaa catctactt 
      ttacaacac ggagctgtc gttgcatgt gataaactt 
      acctcaaaa gatcccacc acacaaacg gtgcaaatt 
      actttcgaa agcacggaa caagctcaa caggccgta 
      aatcaactt aatggtata gaaatagat agtagaccc 
      atcaaagtg gaattggct gtggaaaaa cggggccgt 
      aggggacct cgagggcca ggcgcccct ttcgggggc 
      ctgcctggt cagagtaga caaacggat tttccatta 
      agaatactt gtacaaagc gatatggtt ggagctatt 
      atagggaga cagggatcg acgatacga cagatcact 
      caacagacg agagcgaga gtcgacgtt catagaaaa 
      gacaatgta gggtcgtta gaaaaagcc atcactatt 
      tacggaaat ccggaaaat tgcacaaac gcctgcaaa 
      cgaatttta gaagttatg cagcaagag gccaacaac 
      acgaataaa ggcgaaata tgtttgaaa attctggcg 
      cacaacaac ctcatcgga cgtatcata ggcaagggg 
      ggaaacacg atcaaaagg ataatgcaa gagacggac 
      acgaagatc accgtctcg agcataaac gacataaac 
      tcgttcaat ttggagcga atcatcacc gtgaaagga 
      gccatcgac aatatgagc cgagcggag gcccaaatc 
      agcgccaaa ttacggcag agctacgaa aacgatctc 
      caggcgatg gcgccgcaa acgatgatg ttccccgga 
      ctgcatccg atggcgatg atggccacc gcaggaatt 
      ggttatggt tcaagaggt ctgtacaca ggtcaagcc 
      ccctatccc ggaatgtat cccgcaggt gcggcccag 
      ggcggaggc gacagccag gaaacgacc tatctgtac 
      atacccaac aacgccgtc ggagccatc atcggaacg 
      aaaggctcg catattcga aatatcatt cgattctcg 
      ggagcttcc gtcaagatc gcgcccatc gacgagacg 
      aaaccgcaa gaaacgcaa aacgaaaga agagtcacg 
      attgtcggc tcacccgaa gcgcagtgg aaggcgcag 
      tatttgatt tttgagaag atgcgagag gaaggtttc 
      gtcgctgga tcggatgat gtacgatta acggtggaa 
      ataatggtt cctagctcg caagtcggc cgaatcatc 
      ggcaaagga ggtcaaaat gttcgcgaa ctacaacgc 
      gtaaccgga agcgtaatt aaactgcca gaacagggt 
      gcttctcct caagaggat gaaacaacg gtgcatatc 
      attggacca tttttcagt gttcaatcg gcgcaacgt 
      cgcatacgc gccatggta ttgcaatca gtagtgcct 
      cttcaggga agaccgcgt caaccgcgc caaccaaaa 
      gaccccagc caatcggaa gccgccgct gcgttggac 
      ccacaacag cagcaacag caaacgcca ccgcagcag 
      cagcaacaa caacagcag caaccacca caacaacag 
      taa
    mRNA sequence
    • >TC031140 RA
      ttagtacgc atttagcac gtatttaga gtgatatgt 
      tgtgttacg tcatttttc cgaaaagga ctttttggt 
      ttcgctttc aaaagtgac cctttcggc aacatttgg 
      attttcgtc tcaaataac aacacatcc ggtagtcca 
      tttacgtct gttttgttt tgtttgaat ttttgattt 
      tgatgctgc ttttggtcc ggtggtgat attgtggct 
      atgttgtga cggttttgt gatttctct attttcctg 
      ttggatttt tgtttttgt gtttttacg aacatcaag 
      tttcggtta ttgctcaaa atttcgagt cgatgtctg 
      tttcgttgg tttaatgtt gagtttctt cggaggttt 
      tggcggaca gataaagtt cgttttcga caaagactg 
      attaggctt aagtagtgt gtagtgcgt gtgtggttt 
      agtggtttg ttgccttgt ccatttttg gatactagt 
      gatctaagt acacatttt caacatgga aaagttcca 
      ggaacagca ggaacctga cgatcagga tcaggttta 
      cgaagtcga ggaacctga aactagttc aacatcaaa 
      aattattat atccaattt gggtccaaa cgtccggtt 
      cgaagacat cgaacatct acttttaca acacggagc 
      tgtcgttgc atgtgataa acttacctc aaaagatcc 
      caccacaca aacggtgca aattacttt cgaaagcac 
      ggaacaagc tcaacaggc cgtaaatca acttaatgg 
      tatagaaat agatagtag acccatcaa agtggaatt 
      ggctgtgga aaaacgggg ccgtagggg acctcgagg 
      gccaggcgc ccctttcgg gggcctgcc tggtcagag 
      tagacaaac ggattttcc attaagaat acttgtaca 
      aagcgatat ggttggagc tattatagg gagacaggg 
      atcgacgat acgacagat cactcaaca gacgagagc 
      gagagtcga cgttcatag aaaagacaa tgtagggtc 
      gttagaaaa agccatcac tatttacgg aaatccgga 
      aaattgcac aaacgcctg caaacgaat tttagaagt 
      tatgcagca agaggccaa caacacgaa taaaggcga 
      aatatgttt gaaaattct ggcgcacaa caacctcat 
      cggacgtat cataggcaa ggggggaaa cacgatcaa 
      aaggataat gcaagagac ggacacgaa gatcaccgt 
      ctcgagcat aaacgacat aaactcgtt caatttgga 
      gcgaatcat caccgtgaa aggagccat cgacaatat 
      gagccgagc ggaggccca aatcagcgc caaattacg 
      gcagagcta cgaaaacga tctccaggc gatggcgcc 
      gcaaacgat gatgttccc cggactgca tccgatggc 
      gatgatggc caccgcagg tcaagcccc ctatcccgg 
      aatgtatcc cgcaggtgc ggcccaggg cggaggcga 
      cagccagga aacgaccta tctgtacat acccaacaa 
      cgccgtcgg agccatcat cggaacgaa aggctcgca 
      tattcgaaa tatcattcg attctcggg agcttccgt 
      caagatcgc gcccatcga cgagacgaa accgcaaga 
      aacgcaaaa cgaaagaag agtcacgat tgtcggctc 
      acccgaagc gcagtggaa ggcgcagta tttgatttt 
      tgagaagat gcgagagga aggtttcgt cgctggatc 
      ggatgatgt acgattaac ggtggaaat aatggttcc 
      tagctcgca agtcggccg aatcatcgg caaaggagg 
      tcaaaatgt tcgcgaact acaacgcgt aaccggaag 
      cgtaattaa actgccaga acagggtgc ttctcctca 
      agaggatga aacaacggt gcatatcat tggaccatt 
      tttcagtgt tcaatcggc gcaacgtcg catacgcgc 
      catggtatt gcaatcagt agtgcctct tcagggaag 
      accgcgtca accgcgcca accaaaaga ccccagcca 
      atcggaagc cgccgctgc gttggaccc acaacagca 
      gcaacagca aacgccacc gcagcagca gcaacaaca 
      acagcagca accaccaca acaacagta acgccagct 
      agcaacatt cactgccct gtgcatggg tttatgcat 
      accctattg tacaattaa catctcgag gccttccac 
      taatgcaat atcatcctt ccagtctta gtgtcgtcg 
      tagcgcatt gttgtcgcc gaacacaac caaatatta 
      gctgtgcgc gcgcgtgtt ttaactcta ttaaattaa 
      gtaaaacaa caacaagaa gaagaagaa atgtgtgaa 
      ccgcgaata cctcagcaa gtgctttat tgccgatac 
      gtccatttt gttcagttt gatgatttt ccgctacac 
      tggagttta tttaagtta ttggttcta gtggaaagt 
      ttggagaag aaacaggaa tatggtatg ataaactgt 
      gtgcccaac gttacaatc tacctcaac taatcaaaa 
      taaagttac aagttatag ccatagtaa atttccgtt 
      acgagatac agcaaccaa ccaaccatt caaacctca 
      ctcactcac aaccagcag tgttttctt ttgttgtac 
      acgatctac ctgatgaaa ggactacca aagacaagg 
      cgttgttac aaaccattg tttatcgct tcactggat 
      tttgaccgc tcgcaccta aacaaacta tcgacgaat 
      tcagccaaa acatttcga aagacagga ttttcaacc 
      gagcaagca agagcagtc accgttatc catctgaag 
      agtcccact taaactagc actacatca aaacaaggc 
      gaaagttcc tctgcagag tccggtggc agaggatgc 
      gagacggcc atcacaacg gacaggagt cacaactct 
      tgcatcctc tgcgctgtt aacgtggct gttgttaat 
      tattaagtg tgaatgaag agctttaat aagaaaatt 
      ttgataata ttaatgcga aattgttgt gcgtgtctt 
      tattattat tattgtgtt tgaatttga agagacgtg 
      gcaacgatc tttcaatta attactatt taaaatgga 
      aactgttaa tcaaagtgt gcgtgtgga aaaagacat 
      ttttaactc tattgtaat attattgtt aggagattg 
      ttaaaataa tgtttgata aagatgcgg cacgctctc 
      caattatta ttatttgtt taacggagt gccgaggcc 
      gtgtcgcat ctttgattt ttcttcgtg attgattgt 
      tgctgaatt attatgata ccagatagg acagctgtt 
      gcggccttg ctgccttgc gtcaaggtc ggccgcgcc 
      cctgatagt acaaactcg ggaggtata gattgggcc 
      taagagccc ccggtttag tccggactt gtaaaaaat 
      atttttgat aaaaattgt ttgttgggg aggatattt 
      tttgttaga ttttttaaa aaataatta tagcggggt 
      cgctatttt aaaaaattg tgaaaaaat ggatatatt 
      a
    • >TC031140 RB
      ttagtacgc atttagcac gtatttaga gtgatatgt 
      tgtgttacg tcatttttc cgaaaagga ctttttggt 
      ttcgctttc aaaagtgac cctttcggc aacatttgg 
      attttcgtc tcaaataac aacacatcc ggtagtcca 
      tttacgtct gttttgttt tgtttgaat ttttgattt 
      tgatgctgc ttttggtcc ggtggtgat attgtggct 
      atgttgtga cggttttgt gatttctct attttcctg 
      ttggatttt tgtttttgt gtttttacg aacatcaag 
      tttcggtta ttgctcaaa atttcgagt cgatgtctg 
      tttcgttgg tttaatgtt gagtttctt cggaggttt 
      tggcggaca gataaagtt cgttttcga caaagactg 
      attaggctt aagtagtgt gtagtgcgt gtgtggttt 
      agtggtttg ttgccttgt ccatttttg gatactagt 
      gatctaagt acacatttt caacatgga aaagttcca 
      ggaacagca ggaacctga cgatcagga tcaggttta 
      cgaagtcga ggaacctga aactagttc aacatcaaa 
      aattattat atccaattt gggtccaaa cgtccggtt 
      cgaagacat cgaacatct acttttaca acacggagc 
      tgtcgttgc atgtgataa acttacctc aaaagatcc 
      caccacaca aacggtgca aattacttt cgaaagcac 
      ggaacaagc tcaacaggc cgtaaatca acttaatgg 
      tatagaaat agatagtag acccatcaa agtggaatt 
      ggctgtgga aaaacgggg ccgtagggg acctcgagg 
      gccaggcgc ccctttcgg gggcctgcc tggtcagag 
      tagacaaac ggattttcc attaagaat acttgtaca 
      aagcgatat ggttggagc tattatagg gagacaggg 
      atcgacgat acgacagat cactcaaca gacgagagc 
      gagagtcga cgttcatag aaaagacaa tgtagggtc 
      gttagaaaa agccatcac tatttacgg aaatccgga 
      aaattgcac aaacgcctg caaacgaat tttagaagt 
      tatgcagca agaggccaa caacacgaa taaaggcga 
      aatatgttt gaaaattct ggcgcacaa caacctcat 
      cggacgtat cataggcaa ggggggaaa cacgatcaa 
      aaggataat gcaagagac ggacacgaa gatcaccgt 
      ctcgagcat aaacgacat aaactcgtt caatttgga 
      gcgaatcat caccgtgaa aggagccat cgacaatat 
      gagccgagc ggaggccca aatcagcgc caaattacg 
      gcagagcta cgaaaacga tctccaggc gatggcgcc 
      gcaaacgat gatgttccc cggactgca tccgatggc 
      gatgatggc caccgcagg aattggtta tggttcaag 
      aggtctgta cacaggtca agcccccta tcccggaat 
      gtatcccgc aggtgcggc ccagggcgg aggcgacag 
      ccaggaaac gacctatct gtacatacc caacaacgc 
      cgtcggagc catcatcgg aacgaaagg ctcgcatat 
      tcgaaatat cattcgatt ctcgggagc ttccgtcaa 
      gatcgcgcc catcgacga gacgaaacc gcaagaaac 
      gcaaaacga aagaagagt cacgattgt cggctcacc 
      cgaagcgca gtggaaggc gcagtattt gatttttga 
      gaagatgcg agaggaagg tttcgtcgc tggatcgga 
      tgatgtacg attaacggt ggaaataat ggttcctag 
      ctcgcaagt cggccgaat catcggcaa aggaggtca 
      aaatgttcg cgaactaca acgcgtaac cggaagcgt 
      aattaaact gccagaaca gggtgcttc tcctcaaga 
      ggatgaaac aacggtgca tatcattgg accattttt 
      cagtgttca atcggcgca acgtcgcat acgcgccat 
      ggtattgca atcagtagt gcctcttca gggaagacc 
      gcgtcaacc gcgccaacc aaaagaccc cagccaatc 
      ggaagccgc cgctgcgtt ggacccaca acagcagca 
      acagcaaac gccaccgca gcagcagca acaacaaca 
      gcagcaacc accacaaca acagtaacg ccagctagc 
      aacattcac tgccctgtg catgggttt atgcatacc 
      ctattgtac aattaacat ctcgaggcc ttccactaa 
      tgcaatatc atccttcca gtcttagtg tcgtcgtag 
      cgcattgtt gtcgccgaa cacaaccaa atattagct 
      gtgcgcgcg cgtgtttta actctatta aattaagta 
      aaacaacaa caagaagaa gaagaaatg tgtgaaccg 
      cgaatacct cagcaagtg ctttattgc cgatacgtc 
      cattttgtt cagtttgat gattttccg ctacactgg 
      agtttattt aagttattg gttctagtg gaaagtttg 
      gagaagaaa caggaatat ggtatgata aactgtgtg 
      cccaacgtt acaatctac ctcaactaa tcaaaataa 
      agttacaag ttatagcca tagtaaatt tccgttacg 
      agatacagc aaccaacca accattcaa acctcactc 
      actcacaac cagcagtgt tttcttttg ttgtacacg 
      atctacctg atgaaagga ctaccaaag acaaggcgt 
      tgttacaaa ccattgttt atcgcttca ctggatttt 
      gaccgctcg cacctaaac aaactatcg acgaattca 
      gccaaaaca tttcgaaag acaggattt tcaaccgag 
      caagcaaga gcagtcacc gttatccat ctgaagagt 
      cccacttaa actagcact acatcaaaa caaggcgaa 
      agttcctct gcagagtcc ggtggcaga ggatgcgag 
      acggccatc acaacggac aggagtcac aactcttgc 
      atcctctgc gctgttaac gtggctgtt gttaattat 
      taagtgtga atgaagagc tttaataag aaaattttg 
      ataatatta atgcgaaat tgttgtgcg tgtctttat 
      tattattat tgtgtttga atttgaaga gacgtggca 
      acgatcttt caattaatt actatttaa aatggaaac 
      tgttaatca aagtgtgcg tgtggaaaa agacatttt 
      taactctat tgtaatatt attgttagg agattgtta 
      aaataatgt ttgataaag atgcggcac gctctccaa 
      ttattatta tttgtttaa cggagtgcc gaggccgtg 
      tcgcatctt tgatttttc ttcgtgatt gattgttgc 
      tgaattatt atgatacca gataggaca gctgttgcg 
      gccttgctg ccttgcgtc aaggtcggc cgcgcccct 
      gatagtaca aactcggga ggtatagat tgggcctaa 
      gagcccccg gtttagtcc ggacttgta aaaaatatt 
      tttgataaa aattgtttg ttggggagg atatttttt 
      gttagattt tttaaaaaa taattatag cggggtcgc 
      tattttaaa aaattgtga aaaaatgga tatatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY