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Gene information
General information
TC014047 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4882388..4886171
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014047
    iBeetle screen iB_08233

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: 5 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014047 RA
      MSQKIDWAK FAEEQEKSV QKKLGDVSL SSNRNNSTA 
      EASLLRKLM RNGLVESKT DIEIQRQNP NSPLYSVKT 
      FEALNLNPN LLKGVYDMG FNAPSKIQE TALPTLLAN 
      PPQNLIAQA QSGTGKTAA FVLAMLSRV DASLKYPQV 
      LCLSPTYEL AIQTGEVAA HMAKFCPEI EMKYAVRGE 
      EVSRGSHLT EHIIIGTPG KVLDWALKF RVFDLKKLT 
      VFVLDEADV MIATQGHQD QCIRIHKNL GPNCQMMFF 
      SATYDQQIM EFAEMIVPD SITIRLKRE EESLDNIQQ 
      YYVKCSGPQ EKYNAVTNI YGTVGVGQA IIFCHTRRT 
      ASWLAEKMS KDGHAVAVL TGDLTVEQR INVLDRFRS 
      GQEKVLITT NVLSRGIDV EQVTIVVNF DLPIDMQGK 
      ADCDTYLHR IGRTGRFGK QGIAINLVD SQEAMNICR 
      DIEKHFNRK IHYLNADDS DEIEKIGA
    CDS sequence
    • >TC014047 RA
      atgtctcag aagatagat tgggctaaa tttgcggaa 
      gaacaggaa aaaagcgtc caaaagaag ttgggcgac 
      gttagtctg agttcaaat agaaacaac tccactgcc 
      gaggctagt ttgctgcga aaattgatg cgaaatggt 
      ctcgtggaa agcaaaact gatatcgag atccagagg 
      caaaacccg aactcgccg ctttattct gttaaaact 
      ttcgaagcc ctgaactta aaccccaat ttattgaag 
      ggggtttac gatatgggg ttcaatgcg ccgtcgaaa 
      atccaggaa acggctcta ccgaccctc ttagccaac 
      cctccacag aacctcata gctcaggcc cagtcaggc 
      actgggaaa acggccgct tttgtctta gccatgtta 
      agtcgcgtc gatgcttcg ctaaagtat cctcaagtt 
      ttgtgtttg tcacccact tacgagctg gctatccaa 
      actggcgaa gtggcggca catatggca aagttttgc 
      cccgaaatc gagatgaaa tacgccgtg agaggcgaa 
      gaagtttca agagggtcg catttaacc gaacacatc 
      ataattgga acgcctgga aaagtgcta gattgggcc 
      ctgaaattc cgtgttttc gatctgaaa aaactaaca 
      gtgtttgtc ttggatgag gctgatgtg atgatcgca 
      acccaaggc caccaggat caatgcatc agaatacac 
      aaaaatttg gggccgaat tgtcaaatg atgtttttc 
      tctgcaacg tacgatcaa cagattatg gagtttgct 
      gaaatgatt gttcccgat tctatcaca ataagactg 
      aaacgcgaa gaagaaagt ctggataat atccaacaa 
      tattatgtc aaatgctct ggaccacag gaaaaatat 
      aacgctgtg acgaatatt tatggaact gtgggggtg 
      ggacaggcg atcattttt tgtcacacc agacgcact 
      gcgagctgg ctcgctgaa aaaatgtcg aaagatggc 
      catgctgtg gccgtctta actggagat ttaacggtg 
      gaacaacgt attaacgtc ttagatcgg ttcaggtct 
      ggtcaagaa aaagttctt ataaccacc aatgtattg 
      tctagaggt attgacgtc gaacaagtg acaatcgtt 
      gtaaatttc gatttgcca atagacatg caaggcaag 
      gccgactgc gacacttac ttgcacaga atcggtcga 
      actggacgt tttggtaaa cagggtatt gctataaat 
      ctggttgat tcacaagag gccatgaat atttgtcgc 
      gatatcgag aaacatttt aaccgaaaa attcattat 
      ttgaatgcc gatgattcg gacgaaatt gagaaaatt 
      ggagcctga
    mRNA sequence
    • >TC014047 RA
      tcacacttg aataagaat tctacattt catgtgcta 
      ctgatgatc cgtcacatt cagttcagt tgatgaaag 
      ttagtcttt aaacgtggc gccaagttt taaccaatc 
      gggctcagt ctttaccaa caatcaagt ttgacaaaa 
      tccgatcaa ttttttggg tcatatttg ttgaaaaac 
      aataagaaa aaaaatcaa aaagtttaa tgactagtt 
      tttgtattc aattcagtt gaataaaaa catccttaa 
      ttgtgcatt tgaacttgg cgccatgtt gtgccaaca 
      gtagcgggt gaaaataac taaaccgcg caaaattaa 
      ccacaaatt tgcaaacat gtctcagaa gatagattg 
      ggctaaatt tgcggaaga acaggaaaa aagcgtcca 
      aaagaagtt gggcgacgt tagtctgag ttcaaatag 
      aaacaactc cactgccga ggctagttt gctgcgaaa 
      attgatgcg aaatggtct cgtggaaag caaaactga 
      tatcgagat ccagaggca aaacccgaa ctcgccgct 
      ttattctgt taaaacttt cgaagccct gaacttaaa 
      ccccaattt attgaaggg ggtttacga tatggggtt 
      caatgcgcc gtcgaaaat ccaggaaac ggctctacc 
      gaccctctt agccaaccc tccacagaa cctcatagc 
      tcaggccca gtcaggcac tgggaaaac ggccgcttt 
      tgtcttagc catgttaag tcgcgtcga tgcttcgct 
      aaagtatcc tcaagtttt gtgtttgtc acccactta 
      cgagctggc tatccaaac tggcgaagt ggcggcaca 
      tatggcaaa gttttgccc cgaaatcga gatgaaata 
      cgccgtgag aggcgaaga agtttcaag agggtcgca 
      tttaaccga acacatcat aattggaac gcctggaaa 
      agtgctaga ttgggccct gaaattccg tgttttcga 
      tctgaaaaa actaacagt gtttgtctt ggatgaggc 
      tgatgtgat gatcgcaac ccaaggcca ccaggatca 
      atgcatcag aatacacaa aaatttggg gccgaattg 
      tcaaatgat gtttttctc tgcaacgta cgatcaaca 
      gattatgga gtttgctga aatgattgt tcccgattc 
      tatcacaat aagactgaa acgcgaaga agaaagtct 
      ggataatat ccaacaata ttatgtcaa atgctctgg 
      accacagga aaaatataa cgctgtgac gaatattta 
      tggaactgt gggggtggg acaggcgat cattttttg 
      tcacaccag acgcactgc gagctggct cgctgaaaa 
      aatgtcgaa agatggcca tgctgtggc cgtcttaac 
      tggagattt aacggtgga acaacgtat taacgtctt 
      agatcggtt caggtctgg tcaagaaaa agttcttat 
      aaccaccaa tgtattgtc tagaggtat tgacgtcga 
      acaagtgac aatcgttgt aaatttcga tttgccaat 
      agacatgca aggcaaggc cgactgcga cacttactt 
      gcacagaat cggtcgaac tggacgttt tggtaaaca 
      gggtattgc tataaatct ggttgattc acaagaggc 
      catgaatat ttgtcgcga tatcgagaa acattttaa 
      ccgaaaaat tcattattt gaatgccga tgattcgga 
      cgaaattga gaaaattgg agcctgaat ttttgtaat 
      ttttaagaa cactaccgt ctaattttt taataataa 
      aataataga aagtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY