Status
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Gene information
General information
TC033056 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4705678..4707948
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033056
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08231

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033056 RA
      MRPNILLPL FVIVTYVDG AIFKLQNQT HIHCIDENK 
      DSTILIYPP AETLESSSN LLEDKKWFN DDWEEKETK 
      NLPTAHILD SRWQEIPVI KTNYLHFDL FRSVSISVY 
      TPKNFEIFI YSDTQASYI TPPIENNYW NHFTIISTD 
      KDIITIHNN KTIRKQVNF QPKYVVFKS ENETFLKIH 
      HDRLVRWSN TTSKQPTVL TVPPTESPC MILYSSLCP 
      NCTLHIHHS RKGKKTIHA TAQEPDMIE YWQTTEVEI 
      DPNRENQIT FYRKNMHGQ SSGYWAIEF QLCKKTNMV 
      SYKINSEET PNFTCETLI KTKRKAYEG NIVEPCSSG 
      RFGTYCNIT CESVLGKEF SQCEKCQIC VNETYCYCA 
      SGYKGEKCS KFCEAGKWG PSCRKQCED CSGCDPKTG 
      KCHHPFGKP LGRGQRNKS TAIVLLLTT VAMSMTTVC 
      AVLLICLRL AILYRRDFS AILFQ
    CDS sequence
    • >TC033056 RA
      atgagacct aatatatta ctgcctcta tttgtcatc 
      gtaacttat gttgatggt gctattttc aagttgcaa 
      aaccaaact catattcac tgcattgac gaaaataaa 
      gattcaacc attcttata tatcctccg gctgaaaca 
      ttggaatca tcgtcaaat ttattagaa gataaaaaa 
      tggtttaat gatgactgg gaagaaaaa gaaacaaaa 
      aatttacct acggctcat atattagat agtagatgg 
      caagaaatt ccagttatc aaaaccaac tatttgcat 
      tttgaccta ttccgatct gtttccata tccgtgtat 
      acacccaaa aattttgaa atatttata tatagtgat 
      acacaagcc agctatatc actccacca attgaaaat 
      aactattgg aaccatttt actattata tcaacagac 
      aaagacata atcacaatc cacaacaat aaaactata 
      aggaaacaa gtcaatttt caacccaaa tacgttgtt 
      tttaaaagt gagaacgaa acgtttttg aaaatacat 
      catgatcgg ctggttaga tggtcaaat actacaagc 
      aaacaacca acggtttta acagtacca ccgaccgaa 
      agtccttgc atgatcttg tattcatct ttatgtcca 
      aattgtact ttacatata catcattcc cgtaaagga 
      aagaaaacc atccatgca acagcccaa gaaccagac 
      atgatagaa tactggcaa actactgag gtagaaata 
      gacccaaac agagagaac caaataact ttttacaga 
      aaaaatatg catggccaa tcctctgga tattgggcg 
      atagagttt caattgtgt aaaaagaca aatatggtt 
      agctacaaa atcaattca gaagaaact cctaatttc 
      acttgtgaa accttgatc aagactaaa agaaaagca 
      tatgaaggg aacatagtt gaaccatgt agttctgga 
      agatttgga acatactgc aacataact tgtgaaagt 
      gtactcggc aaagaattt tcacagtgt gagaaatgt 
      cagatttgt gtaaacgaa acatactgt tactgtgct 
      tcaggatac aaaggagaa aagtgctct aaattttgt 
      gaagctgga aaatgggga ccgtcgtgt agaaagcaa 
      tgcgaagat tgcagtgga tgtgaccct aaaaccgga 
      aaatgtcat catccattc ggtaaaccc cttggtaga 
      ggccaaaga aataaaagt acagcaatc gttctgttg 
      ttgactacc gtggctatg tcaatgaca acggtgtgc 
      gcggtgtta ctaatttgt ctacgcttg gcaatacta 
      taccgacga gacttcagt gctattctt ttccaatga
    mRNA sequence
    • >TC033056 RA
      atataaata caaatacga gtaggtact aacgtagtt 
      ttacattca aagtgtact cgaaataaa tatctgtca 
      agacgaagt agtttttag tggtattgt tgtaaatac 
      tgatccaca cagtggcca ggaattgcc tttaaacca 
      aagagccac ctgacaata tttacagta taaataaaa 
      atgttcctg tttttggaa aaattattt tgcaatgag 
      acctaatat attactgcc tctatttgt catcgtaac 
      ttatgttga tggtgctat tttcaagtt gcaaaacca 
      aactcatat tcactgcat tgacgaaaa taaagattc 
      aaccattct tatatatcc tccggctga aacattgga 
      atcatcgtc aaatttatt agaagataa aaaatggtt 
      taatgatga ctgggaaga aaaagaaac aaaaaattt 
      acctacggc tcatatatt agatagtag atggcaaga 
      aattccagt tatcaaaac caactattt gcattttga 
      cctattccg atctgtttc catatccgt gtatacacc 
      caaaaattt tgaaatatt tatatatag tgatacaca 
      agccagcta tatcactcc accaattga aaataacta 
      ttggaacca ttttactat tatatcaac agacaaaga 
      cataatcac aatccacaa caataaaac tataaggaa 
      acaagtcaa ttttcaacc caaatacgt tgtttttaa 
      aagtgagaa cgaaacgtt tttgaaaat acatcatga 
      tcggctggt tagatggtc aaatactac aagcaaaca 
      accaacggt tttaacagt accaccgac cgaaagtcc 
      ttgcatgat cttgtattc atctttatg tccaaattg 
      tactttaca tatacatca ttcccgtaa aggaaagaa 
      aaccatcca tgcaacagc ccaagaacc agacatgat 
      agaatactg gcaaactac tgaggtaga aatagaccc 
      aaacagaga gaaccaaat aacttttta cagaaaaaa 
      tatgcatgg ccaatcctc tggatattg ggcgataga 
      gtttcaatt gtgtaaaaa gacaaatat ggttagcta 
      caaaatcaa ttcagaaga aactcctaa tttcacttg 
      tgaaacctt gatcaagac taaaagaaa agcatatga 
      agggaacat agttgaacc atgtagttc tggaagatt 
      tggaacata ctgcaacat aacttgtga aagtgtact 
      cggcaaaga attttcaca gtgtgagaa atgtcagat 
      ttgtgtaaa cgaaacata ctgttactg tgcttcagg 
      atacaaagg agaaaagtg ctctaaatt ttgtgaagc 
      tggaaaatg gggaccgtc gtgtagaaa gcaatgcga 
      agattgcag tggatgtga ccctaaaac cggaaaatg 
      tcatcatcc attcggtaa accccttgg tagaggcca 
      aagaaataa aagtacagc aatcgttct gttgttgac 
      taccgtggc tatgtcaat gacaacggt gtgcgcggt 
      gttactaat ttgtctacg cttggcaat actataccg 
      acgagactt cagtgctat tcttttcca atgaaaaaa 
      tgattagta aaatgcatt tagggtttc tttgggctt 
      gcttcggct tagaggccc catatctgt tcatggcaa 
      catatgttt tgaaaagtg tgacgaagt gagcaataa 
      acgtcttta cccgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY