Status
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Gene information
General information
TC014022 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4374142..4375695
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014022
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08225

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature random rounded - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014022 RA
      MRLEAFLLT FGLISGAKP SETTFENVT IWKSAEEEL 
      AVIVSSNSL KQHLPNTSL DLIAITGEI PILYENSLS 
      NISNVEQIY FHENELTEI KPGAFKNLT LEALEVFYS 
      NLTTLKSGI FCNVSLEKL EISYNFLSK IEPFGGLTG 
      LRVLSLSHN NLEDLEAHL LDKLPELQV LDLSYNRIS 
      VLHPKLLNN QCKLKTLLL RKNKFWHFP NIFENSSLE 
      LLELSSNLM SKLPEGVLD NLQNVSRLF LSFNHLESI 
      PSNVFTGHP NIKELDLSN NRITTIASD AFDNMPQLV 
      NIKFTSNKL AKYDPNWFH RTPNLYQLN LANNQIGDL 
      PEEVFRNLY SDKKQWISL SGNKIKTIS PNAFRGFKR 
      FDTLNLSFN ELESWDGSL LADTEVIFY LNLLHNKIK 
      CVGGDLDQA FKANFTYLG MNPLAPRCL QKILDWRGD 
      KHVVCTAPC LLY
    CDS sequence
    • >TC014022 RA
      atgcgtctc gaagcgttt cttttaact ttcggtcta 
      atatccggt gctaaaccg agtgaaaca acgtttgaa 
      aatgtgaca atttggaaa agtgcggag gaagaactg 
      gcagtgatt gtgtcctca aacagcctc aaacagcac 
      ctccccaac acctcgctc gacctaatc gcaataacg 
      ggggaaata cccattttg tacgaaaac tcactttca 
      aacatctcg aacgtggaa caaatctac ttccacgaa 
      aacgaatta accgaaatc aagccggga gccttcaaa 
      aacttgact ctggaggct ttggaggtg ttttacagc 
      aatttgaca acactcaag agcgggatt ttctgtaac 
      gtgtctttg gaaaaactc gaaattagt tacaatttc 
      ctttcgaaa atcgagccc tttgggggt ttaaccggt 
      ttgagggtt ttgtctctg tctcataac aatttggag 
      gacttggag gcgcacctt ttggacaaa ctccccgag 
      ctgcaagtg ttggatttg tcgtacaac cgcatttcg 
      gtgcttcac ccaaagttg ctcaacaat cagtgcaag 
      ttgaaaacc ctcttgttg cggaaaaat aagttctgg 
      cacttcccc aacattttt gaaaactct tcattggaa 
      ttactggaa ctgtcgtcg aacttaatg tcgaaactt 
      cctgaaggt gttttggat aatttgcaa aacgtgtca 
      cgtctcttc ctctcgttc aaccacctt gagtcgatt 
      ccttccaat gtttttacc ggccaccca aatatcaaa 
      gaattggat ttgtcgaat aacaggatc acaaccatt 
      gcgtcagac gccttcgac aacatgccc cagctagtc 
      aacatcaaa tttacgagt aataaactg gcaaagtac 
      gacccaaac tggtttcac cgcactccg aatttgtac 
      caattgaat ttggccaac aaccagatt ggggacctc 
      ccagaggaa gtcttcagg aatctgtac agcgacaag 
      aagcagtgg atttctcta agtggcaat aaaatcaag 
      acgatttct ccgaacgcc ttcagaggt tttaagcgt 
      ttcgacact ttgaacttg tcgtttaac gagctggag 
      agctgggac gggagtctt ctggccgac accgaagtg 
      attttctat ttgaatctg ttgcacaat aaaattaaa 
      tgcgtggga ggtgatctc gaccaagct tttaaggcc 
      aatttcacg tatttggga atgaacccg ttggcgccc 
      cggtgcttg cagaaaatc ctcgattgg agaggagac 
      aagcacgtg gtgtgtacg gccccttgt ttgttgtat 
      taa
    mRNA sequence
    • >TC014022 RA
      ttattcctc acaataatg cgtctcgaa gcgtttctt 
      ttaactttc ggtctaata tccggtgct aaaccgagt 
      gaaacaacg tttgaaaat gtgacaatt tggaaaagt 
      gcggaggaa gaactggca gtgattgtg tcctcaaac 
      agcctcaaa cagcacctc cccaacacc tcgctcgac 
      ctaatcgca ataacgggg gaaataccc attttgtac 
      gaaaactca ctttcaaac atctcgaac gtggaacaa 
      atctacttc cacgaaaac gaattaacc gaaatcaag 
      ccgggagcc ttcaaaaac ttgactctg gaggctttg 
      gaggtgttt tacagcaat ttgacaaca ctcaagagc 
      gggattttc tgtaacgtg tctttggaa aaactcgaa 
      attagttac aatttcctt tcgaaaatc gagcccttt 
      gggggttta accggtttg agggttttg tctctgtct 
      cataacaat ttggaggac ttggaggcg caccttttg 
      gacaaactc cccgagctg caagtgttg gatttgtcg 
      tacaaccgc atttcggtg cttcaccca aagttgctc 
      aacaatcag tgcaagttg aaaaccctc ttgttgcgg 
      aaaaataag ttctggcac ttccccaac atttttgaa 
      aactcttca ttggaatta ctggaactg tcgtcgaac 
      ttaatgtcg aaacttcct gaaggtgtt ttggataat 
      ttgcaaaac gtgtcacgt ctcttcctc tcgttcaac 
      caccttgag tcgattcct tccaatgtt tttaccggc 
      cacccaaat atcaaagaa ttggatttg tcgaataac 
      aggatcaca accattgcg tcagacgcc ttcgacaac 
      atgccccag ctagtcaac atcaaattt acgagtaat 
      aaactggca aagtacgac ccaaactgg tttcaccgc 
      actccgaat ttgtaccaa ttgaatttg gccaacaac 
      cagattggg gacctccca gaggaagtc ttcaggaat 
      ctgtacagc gacaagaag cagtggatt tctctaagt 
      ggcaataaa atcaagacg atttctccg aacgccttc 
      agaggtttt aagcgtttc gacactttg aacttgtcg 
      tttaacgag ctggagagc tgggacggg agtcttctg 
      gccgacacc gaagtgatt ttctatttg aatctgttg 
      cacaataaa attaaatgc gtgggaggt gatctcgac 
      caagctttt aaggccaat ttcacgtat ttgggaatg 
      aacccgttg gcgccccgg tgcttgcag aaaatcctc 
      gattggaga ggagacaag cacgtggtg tgtacggcc 
      ccttgtttg ttgtattaa acagggacg tagctagct 
      cttaactac agaaagggc acattattt tgtagaagg 
      gccaagtaa atctaaatg tagactagt attaaagtc 
      aagaagaag gtcgagatg aagaaaggg actgagtag 
      gcaacattt aatttttag tttgacgtg tgacacaag 
      atgtgtagg aactttcaa actcaatat acagacgca 
      caaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY