Status
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Gene information
General information
TC013997 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3761451..3772025
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013997
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013997 RA
      MFASICVRN GDTLIINDD CYEILQEIL RELLTEDST 
      LRTYRRAIG FGQNIKKDL IPLLTYLKD DAKESTRII 
      DTIIKILVN LTIPIEYLL PLEAMSQTD VGRHTIFEL 
      TKLLTSSKE AFTDSRSTK LIMDHVKFI VESDLEMNL 
      EYCDSINNC LLLLRNILH VPEVKSVQN NALTHSSMQ 
      NQIIWNLFT QSIDKVIIY LISCPQKMY WGVTMVQLI 
      ALLYKDQHV GTLQKLLNL WLEASLSES SEDNESNTS 
      PPEQGSDDS SPIITSDPT SDSSDGGGG KRNKTLNST 
      NLERNKKTL MMRGKSTET ETSTSSGVS TAHTSIDSA 
      DSPKNDKAP LKNKRECPS SQSEDFGYV TQVENQESI 
      STSSNEEDQ PQLKPVHQK PHTFQKSRY NTGKSRAAT 
      ALDKKELRR KKLVKRSKT NTINMKGLL HHLPTDEDI 
      ANILKEFTV DFLLKGYGI LVQDLHSQF LTNIHLQID 
      TSHFFWLVT YFLKFATQL ELDLEHVSP VLSHEILSY 
      LVFQGVWIY EELEMSGKI PDFDLKPCL RRLHLVVTA 
      IREFLQAVE TYQKMIHLS EDDRLALLK LQNQITEME 
      DLRSLFLLL LRQYNPNIQ SRQYLQDLI LTNHNYLLF 
      LDSATKERR SFNMMEHLK QFATVEIMR QYGLLLESF 
      KENGEFVNN CIFTMMHHI GGDLEHVAT LFQPSILKT 
      FSLIWETDY EICDDWSDL IEYIIHKFI NSPRSNSDL 
      NSSETCHKL VVDEAEWTE EEKDNLLGY YNQSQFSND 
      TIANITKKY EKCGVRQKT QLSIIRQLL EQNIINESQ 
      YNDFIKVKQ QPDRLKETK NIIPNQMDF EETPTDEGI 
      RILREYLCK DNKTKFVLW LQKVLINTC YVKLALANP 
      GEFNNDKAL GQPVIYYFA LLNLPIPVV PWTVDQTAI 
      LKYQSFVLL LHKLGFYLP IDTGKIFVT IPNFWNAEH 
      VFSMAQQLG PIDPAKLNF DVAIFKGRN RRELFKREI 
      TDITNSIQT GKFSMNDSK QTPTMVRYT PLPNSTEWF 
      QNDNFQSRV LPIADFEVP INSAHPLSI TIPGLEELT 
      CDLPPPELS IVDNHVINA NMECSPIHS ACETASIAS 
      DLTRMCVSD EEEKTILVI EQK
    CDS sequence
    • >TC013997 RA
      atgtttgct tcaatctgt gtccgaaat ggggacaca 
      cttataata aacgacgac tgctacgaa atattacaa 
      gaaatcctg agggagctt ttaaccgaa gacagtact 
      ttaaggacg tacagaaga gccatcggt ttcgggcaa 
      aacatcaaa aaggaccta atcccactc ctcacgtac 
      ttgaaagac gatgcgaaa gaatcgacg agaattatc 
      gatacgatt atcaaaatt ttggttaat ctgacgatt 
      ccgatcgaa tatctattg ccgctagaa gccatgtct 
      caaacggat gtcgggcga cacaccatt tttgagctc 
      actaaacta ctgactagc agtaaggaa gcttttact 
      gatagcaga agcacaaaa ctgatcatg gatcacgtc 
      aaatttatc gtcgaaagc gacttggag atgaacttg 
      gagtattgt gacagcatc aataattgt cttctactt 
      ctgcggaac attctccac gtacccgaa gtgaaaagc 
      gtccaaaac aacgctttg acgcactcc agcatgcaa 
      aatcaaatc atatggaac ttgttcaca caaagcatc 
      gataaagta atcatttat ctaatttcg tgtccacaa 
      aaaatgtac tggggggtg acaatggtc caactcatc 
      gcgctcctc tacaaggac caacacgtg gggaccctc 
      caaaaactc ctcaacctg tggctggaa gcctccctc 
      agcgagagc tccgaagac aacgagagc aacacctcg 
      cccccggag caaggcagc gacgactcc tcccccatc 
      atcacctcg gaccccacc tccgactca agcgacggt 
      ggcggaggc aaaagaaac aaaactcta aactcgacc 
      aatctggaa cgcaacaag aaaaccctg atgatgcgc 
      ggcaagtcc accgaaacg gagacgagc acaagcagc 
      ggcgtctcg acggcacac accagcata gactcggcc 
      gacagtccc aaaaatgac aaagccccc ttgaagaac 
      aaaagggaa tgtccctca tcgcaaagc gaagatttt 
      gggtacgtg acccaagtg gagaaccag gagtccatc 
      tccacgtcc agtaacgag gaggaccaa ccccagctg 
      aaaccggtg caccagaag ccccacacg tttcagaaa 
      agtcgctac aatacgggg aaaagtcgc gccgctacg 
      gcgctagac aagaaggag ttgcgcagg aagaagttg 
      gtcaagagg agtaaaact aacacgatc aacatgaag 
      ggcctgttg caccacctg cccaccgac gaggacatt 
      gcgaacatc ctcaaagag ttcaccgtg gatttcctc 
      ctcaagggt tacggtatc ctggtgcaa gacctccac 
      tcgcaattc ctcacaaat atccacctc caaatcgac 
      acttctcac ttcttctgg ctcgttacg tacttcctc 
      aaattcgct acgcagtta gagttagac ctggaacac 
      gtcagtccc gtgttatcg cacgaaatc ctctcctat 
      ctcgtcttt caaggcgtt tggatttac gaagagctg 
      gaaatgagc gggaaaatc cccgatttt gacctgaaa 
      ccgtgtctg aggaggctc cacttggtg gtgacggca 
      attcgggag ttccttcag gccgtggag acgtaccag 
      aaaatgatt cacttgagt gaggacgac cggcttgcg 
      ctcttgaag ctccaaaat cagatcacc gaaatggaa 
      gacttgagg tcactgttt ttgctgttg ttgagacag 
      tacaatccc aacattcaa agcagacag tacctccag 
      gatctgatt ttgacaaat cacaactat ttgttgttt 
      ttggattca gcgaccaaa gagcgacgc tcttttaat 
      atgatggaa cacctcaaa cagttcgcg accgtcgaa 
      attatgaga cagtacgga ctgttgctt gagagtttc 
      aaagagaac ggcgaattt gtcaataat tgcattttt 
      acgatgatg caccacatc gggggcgac ctcgagcac 
      gttgccacg cttttccag ccgagcatc ctcaaaacg 
      ttctccctc atttgggaa accgactac gaaatttgc 
      gatgactgg tccgattta atcgaatac ataatccac 
      aaatttata aattcgcca cgttccaac tctgattta 
      aactcgtca gagacttgc cacaaactg gtcgtggac 
      gaagccgag tggaccgaa gaagagaaa gacaatttg 
      ctgggttat tacaaccag agtcagttt tccaacgac 
      acgatcgca aatataaca aaaaaatac gagaaatgt 
      ggagttcgt caaaagaca caattatcc ataatccgg 
      caattgttg gaacaaaac ataatcaac gagagccaa 
      tataacgac ttcatcaaa gtgaaacaa caacccgat 
      cggttgaaa gaaaccaaa aacattatt ccgaaccaa 
      atggacttt gaagagacg ccaactgat gaaggaatt 
      cgaattctg cgcgaatat ttgtgcaaa gacaacaaa 
      acaaaattc gtcttgtgg ctccaaaag gtcctaatc 
      aacacttgt tacgtcaag ttggctcta gcaaacccc 
      ggggaattc aacaacgac aaagcttta ggtcaacct 
      gtcatctac tactttgct ctactgaat ttgccaatc 
      ccggtggtt ccctggacc gtcgaccaa accgccatt 
      ttgaaatac cagtcgttt gtgctactt ttgcacaag 
      ctgggtttt tacttaccc atcgatact ggaaaaatc 
      ttcgtaaca atccccaat ttttggaac gccgaacat 
      gtcttctca atggcgcag cagctgggg cctatcgat 
      ccagcgaaa ctcaatttc gatgttgcg atttttaaa 
      gggcgcaat cggcgcgaa ttgttcaaa cgcgaaatt 
      accgatatt acgaattcc atccagacg gggaagttc 
      tcaatgaac gactcgaaa caaacgccg acgatggtc 
      agatatacg cccttaccg aattcgact gaatggttt 
      caaaacgat aacttccag tcgagggta cttccaatc 
      gctgatttc gaagtaccg attaatagt gctcatcct 
      ctttcgata acgatcccg ggcctcgaa gagctcact 
      tgtgactta cctcccccc gaattaagt atagttgac 
      aatcacgtg atcaacgca aacatggaa tgttcccca 
      attcacagc gcgtgcgag accgcttcg atcgcttcc 
      gacttgacc cgaatgtgt gtaagtgac gaagaagag 
      aaaacaatt ttagttatc gaacaaaaa taa
    mRNA sequence
    • >TC013997 RA
      tgatgtata gctacaagt cgctcattt tttccagtg 
      ttgatgttc ggtgtgaat cccacaaat ggcccatat 
      tcacaacat gtttgcttc aatctgtgt ccgaaatgg 
      ggacacact tataataaa cgacgactg ctacgaaat 
      attacaaga aatcctgag ggagctttt aaccgaaga 
      cagtacttt aaggacgta cagaagagc catcggttt 
      cgggcaaaa catcaaaaa ggacctaat cccactcct 
      cacgtactt gaaagacga tgcgaaaga atcgacgag 
      aattatcga tacgattat caaaatttt ggttaatct 
      gacgattcc gatcgaata tctattgcc gctagaagc 
      catgtctca aacggatgt cgggcgaca caccatttt 
      tgagctcac taaactact gactagcag taaggaagc 
      ttttactga tagcagaag cacaaaact gatcatgga 
      tcacgtcaa atttatcgt cgaaagcga cttggagat 
      gaacttgga gtattgtga cagcatcaa taattgtct 
      tctacttct gcggaacat tctccacgt acccgaagt 
      gaaaagcgt ccaaaacaa cgctttgac gcactccag 
      catgcaaaa tcaaatcat atggaactt gttcacaca 
      aagcatcga taaagtaat catttatct aatttcgtg 
      tccacaaaa aatgtactg gggggtgac aatggtcca 
      actcatcgc gctcctcta caaggacca acacgtggg 
      gaccctcca aaaactcct caacctgtg gctggaagc 
      ctccctcag cgagagctc cgaagacaa cgagagcaa 
      cacctcgcc cccggagca aggcagcga cgactcctc 
      ccccatcat cacctcgga ccccacctc cgactcaag 
      cgacggtgg cggaggcaa aagaaacaa aactctaaa 
      ctcgaccaa tctggaacg caacaagaa aaccctgat 
      gatgcgcgg caagtccac cgaaacgga gacgagcac 
      aagcagcgg cgtctcgac ggcacacac cagcataga 
      ctcggccga cagtcccaa aaatgacaa agccccctt 
      gaagaacaa aagggaatg tccctcatc gcaaagcga 
      agattttgg gtacgtgac ccaagtgga gaaccagga 
      gtccatctc cacgtccag taacgagga ggaccaacc 
      ccagctgaa accggtgca ccagaagcc ccacacgtt 
      tcagaaaag tcgctacaa tacggggaa aagtcgcgc 
      cgctacggc gctagacaa gaaggagtt gcgcaggaa 
      gaagttggt caagaggag taaaactaa cacgatcaa 
      catgaaggg cctgttgca ccacctgcc caccgacga 
      ggacattgc gaacatcct caaagagtt caccgtgga 
      tttcctcct caagggtta cggtatcct ggtgcaaga 
      cctccactc gcaattcct cacaaatat ccacctcca 
      aatcgacac ttctcactt cttctggct cgttacgta 
      cttcctcaa attcgctac gcagttaga gttagacct 
      ggaacacgt cagtcccgt gttatcgca cgaaatcct 
      ctcctatct cgtctttca aggcgtttg gatttacga 
      agagctgga aatgagcgg gaaaatccc cgattttga 
      cctgaaacc gtgtctgag gaggctcca cttggtggt 
      gacggcaat tcgggagtt ccttcaggc cgtggagac 
      gtaccagaa aatgattca cttgagtga ggacgaccg 
      gcttgcgct cttgaagct ccaaaatca gatcaccga 
      aatggaaga cttgaggtc actgttttt gctgttgtt 
      gagacagta caatcccaa cattcaaag cagacagta 
      cctccagga tctgatttt gacaaatca caactattt 
      gttgttttt ggattcagc gaccaaaga gcgacgctc 
      ttttaatat gatggaaca cctcaaaca gttcgcgac 
      cgtcgaaat tatgagaca gtacggact gttgcttga 
      gagtttcaa agagaacgg cgaatttgt caataattg 
      catttttac gatgatgca ccacatcgg gggcgacct 
      cgagcacgt tgccacgct tttccagcc gagcatcct 
      caaaacgtt ctccctcat ttgggaaac cgactacga 
      aatttgcga tgactggtc cgatttaat cgaatacat 
      aatccacaa atttataaa ttcgccacg ttccaactc 
      tgatttaaa ctcgtcaga gacttgcca caaactggt 
      cgtggacga agccgagtg gaccgaaga agagaaaga 
      caatttgct gggttatta caaccagag tcagttttc 
      caacgacac gatcgcaaa tataacaaa aaaatacga 
      gaaatgtgg agttcgtca aaagacaca attatccat 
      aatccggca attgttgga acaaaacat aatcaacga 
      gagccaata taacgactt catcaaagt gaaacaaca 
      acccgatcg gttgaaaga aaccaaaaa cattattcc 
      gaaccaaat ggactttga agagacgcc aactgatga 
      aggaattcg aattctgcg cgaatattt gtgcaaaga 
      caacaaaac aaaattcgt cttgtggct ccaaaaggt 
      cctaatcaa cacttgtta cgtcaagtt ggctctagc 
      aaaccccgg ggaattcaa caacgacaa agctttagg 
      tcaacctgt catctacta ctttgctct actgaattt 
      gccaatccc ggtggttcc ctggaccgt cgaccaaac 
      cgccatttt gaaatacca gtcgtttgt gctactttt 
      gcacaagct gggttttta cttacccat cgatactgg 
      aaaaatctt cgtaacaat ccccaattt ttggaacgc 
      cgaacatgt cttctcaat ggcgcagca gctggggcc 
      tatcgatcc agcgaaact caatttcga tgttgcgat 
      ttttaaagg gcgcaatcg gcgcgaatt gttcaaacg 
      cgaaattac cgatattac gaattccat ccagacggg 
      gaagttctc aatgaacga ctcgaaaca aacgccgac 
      gatggtcag atatacgcc cttaccgaa ttcgactga 
      atggtttca aaacgataa cttccagtc gagggtact 
      tccaatcgc tgatttcga agtaccgat taatagtgc 
      tcatcctct ttcgataac gatcccggg cctcgaaga 
      gctcacttg tgacttacc tccccccga attaagtat 
      agttgacaa tcacgtgat caacgcaaa catggaatg 
      ttccccaat tcacagcgc gtgcgagac cgcttcgat 
      cgcttccga cttgacccg aatgtgtgt aagtgacga 
      agaagagaa aacaatttt agttatcga acaaaaata 
      attttggtg tgtttgagt tgctttgaa tgtgttaat 
      tttggtaca taaataact gttatgttg actttttat 
      atgtgacta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY