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Gene information
General information
TC001630 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1276571..1279227
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001630
    iBeetle screen iB_08214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence broader - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: majority of hatched larvae on slide is dead
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001630 RA
      MFTNLEKFG LVCISVVGF QIFRFLFRT LYNNFLAVA 
LKINAVDVK ETGSWAVVT GATDGIGKA YAELLAKKG 
LNIVLISRT REKLEKVAN EIASKYHVE TKIIEADFT 
QTDPIYTHI DKQLTGLEI GVLINNVGM SYPHPEYFL 
DLKNKDEVY NNIINCNIY SVTNMCKIV LPGMVERRR 
GVVVNLSST AAQIPSPLL TVYAATKAY VEKFSQDLN 
SEYSKFGIT IQCILPGYV ATNMSKIRS STWMAPSPL 
KFVKEAMKT IGVLERTTG YYPHTLLVG VIHTLEAVS 
PRLSRWLVI RTMENIRAR ALKRINH
    CDS sequence
    • >TC001630 RA
      atgttcact aatttagaa aaattcggt ttggtgtgc 
atttcggtc gtagggttt caaattttc cgattttta 
ttccgaacc ctttacaat aattttttg gctgtggcg 
ttgaaaata aacgcagtc gatgtcaaa gagacagga 
agttgggca gtcgttacc ggcgctact gatggaatt 
ggcaaagcc tatgccgag cttttggcc aaaaagggc 
cttaacata gtcctaatc agccggacg cgcgaaaaa 
cttgaaaaa gtcgcaaat gaaattgct tcaaaatat 
cacgtggag accaaaata atcgaagcc gacttcacc 
caaaccgac ccgatttac acccacatt gataagcaa 
ttgactggc ttagaaatc ggggtttta ataaacaac 
gtgggtatg agctacccc caccccgag tatttctta 
gatttgaaa aacaaagac gaagtttac aataacata 
atcaattgc aacatttat tcggtcaca aatatgtgc 
aaaatcgtc ttacctgga atggtcgag cgacgtcgg 
ggtgttgtg gttaattta tcctccact gcggcccaa 
atcccgagc cccctcttg acggtttac gccgcgacg 
aaagcctac gttgaaaaa ttctcccag gacttgaac 
tccgaatac tccaagttt gggattacc atccagtgc 
attctcccg gggtatgtc gccacaaat atgtccaaa 
atacgcagc tcgacgtgg atggccccc tcgccgctg 
aaattcgtc aaagaagcc atgaaaaca atcggggtt 
ttggagcgc acgactggg tattacccc catacgctg 
ctcgtgggg gttatccat accctggaa gcggtgtca 
ccgcgactc tccaggtgg ctggtgatc agaactatg 
gagaacatc cgagcgcgt gcattgaaa cggattaac 
cactaa
    mRNA sequence
    • >TC001630 RA
      tcagagtaa ctatagtgc ttcatattg tactcgcga 
tttgaaata attaggtgt ttacagtgc tcgcacatt 
aaaatgttc actaattta gaaaaattc ggtttggtg 
tgcatttcg gtcgtaggg tttcaaatt ttccgattt 
ttattccga accctttac aataatttt ttggctgtg 
gcgttgaaa ataaacgca gtcgatgtc aaagagaca 
ggaagttgg gcagtcgtt accggcgct actgatgga 
attggcaaa gcctatgcc gagcttttg gccaaaaag 
ggccttaac atagtccta atcagccgg acgcgcgaa 
aaacttgaa aaagtcgca aatgaaatt gcttcaaaa 
tatcacgtg gagaccaaa ataatcgaa gccgacttc 
acccaaacc gacccgatt tacacccac attgataag 
caattgact ggcttagaa atcggggtt ttaataaac 
aacgtgggt atgagctac ccccacccc gagtatttc 
ttagatttg aaaaacaaa gacgaagtt tacaataac 
ataatcaat tgcaacatt tattcggtc acaaatatg 
tgcaaaatc gtcttacct ggaatggtc gagcgacgt 
cggggtgtt gtggttaat ttatcctcc actgcggcc 
caaatcccg agccccctc ttgacggtt tacgccgcg 
acgaaagcc tacgttgaa aaattctcc caggacttg 
aactccgaa tactccaag tttgggatt accatccag 
tgcattctc ccggggtat gtcgccaca aatatgtcc 
aaaatacgc agctcgacg tggatggcc ccctcgccg 
ctgaaattc gtcaaagaa gccatgaaa acaatcggg 
gttttggag cgcacgact gggtattac ccccatacg 
ctgctcgtg ggggttatc cataccctg gaagcggtg 
tcaccgcga ctctccagg tggctggtg atcagaact 
atggagaac atccgagcg cgtgcattg aaacggatt 
aaccactaa ttgtttgat tatttattt aataaaggg 
ttgttacaa at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY