| General information | |||
| TC033029
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 3251167..3263933 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033029 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08211 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 2
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033029 RA
MSSQRFCLR WNNHQSNLL SVFDQLLHD ESFVDVTLA VEGQLLRAH KMVLSACSP YFQALFVNH PDKHPIVIL KDVPYSDMR SLLDFMYRG EVSVDQDRL TAFLRVAES LRIKGLTEV NEEKCDTIT SSLTQQNQM NANMPHLQR LQQNKRFGN PLNMLGNAL LQPKRKRGR PRKLSGSSN GTPGEDFDR GDALVQGSP EMLEVKMGM DGFTGNNSD CSTGKSDAK ENDLDDNNT KAKEENEPV AGTSKEQNT FNDTKNADD FQSSGDKDR SSPNNIHCI DFNSSLHEE YNLPYQDTS TSDIELDDL DSNYETLDI KLDPKMVLD GFSDKKKRK SLSLMDVED FASYDIVLD DPSYDFKTL KYVNYSKKI SDPDKSARD FCVREKDNL YRCTVCGRV YTHISNFCR HYMTSHKLD VKMFSCPVC CKDFTRKDN MLAHLKIIH KQNMS
- >TC033029 RB
MSSQRFCLR WNNHQSNLL SVFDQLLHD ESFVDVTLA VEGQLLRAH KMVLSACSP YFQALFVNH PDKHPIVIL KDVPYSDMR SLLDFMYRG EVSVDQDRL TAFLRVAES LRIKGLTEV NEEKCDTIT SSLTQQNQM NANMPHLQR LQQNKRFGN PLNMLGNAL LQPKRKRGR PRKLSGSSN GTPGEDFDR GDALVQGSP EMLEVKMGM DGFTGNNSD CSTGKSDAK ENDLDDNNT KAKEENEPV AGTSKEQNT FNDTKNADD FQSSDSPQA GKLLSPKQE PGEGATQEP RLSASDSLN NNAPASSGA PMSAPTSGR QPVKRRARR RATSQSQDP AEQLTEMSV RGLNLFRYA SINEGVYQC TECAKVDVQ KTFKNKYSF QRHAFLYHE GHQRKVFPC PVCQKEFSR PDKMKNHMK TVHDCFMPK DVVFPPMGF FPIP
- >TC033029 RA
atgtcttcc caacgtttc tgtctgaga tggaacaac catcagtcg aatttgttg tcagtattc gaccaactc ctacacgac gaaagtttc gtcgatgtc acgttggcc gtggaagga caattgttg cgtgcccac aaaatggtc ctttcggcg tgcagtccc tacttccag gcgctgttc gtcaaccat cccgacaaa caccccatc gttatcctt aaggacgtc ccatacagc gatatgcgc tccttactg gacttcatg tacagaggg gaggtcagc gtcgaccag gacagactc acagccttc ctgcgggtc gctgaaagt ctgcggata aagggtctg acagaggtg aacgaagag aaatgtgat acaataacg tcgtctttg acgcaacaa aaccaaatg aacgccaac atgccacat ctgcaaaga ctgcaacag aacaagagg ttcggcaac ccgctcaac atgctcggc aatgctctt ctccagccg aagaggaag cgcggacgg cctcggaaa ctctcgggg agttcgaac ggcacccca ggcgaggac ttcgacagg ggcgatgcc ctggtgcag ggcagcccc gaaatgctc gaagtcaaa atgggaatg gacggattc acgggaaac aactccgac tgcagcacg ggcaagtcg gacgcgaag gaaaacgat ctggacgat aacaatacc aaagctaag gaagaaaac gaaccggtc gccggcacc tccaaggaa cagaacacg tttaacgac acgaaaaac gccgacgat tttcagtct tccggcgac aaagaccgt tcctccccc aacaatatc cactgtata gactttaac tcgtcgctg cacgaagag tacaatttg ccatatcag gacacctcc acatccgac atagagtta gacgatttg gactcgaac tatgagact ctcgatatt aaactagat ccaaagatg gtcctcgac ggcttctcc gacaagaag aaacgcaaa agtttgagt cttatggac gtggaggac tttgccagc tatgatata gtcctggac gacccctcc tacgacttc aaaaccctc aaatacgtc aattattcg aaaaaaatc agcgacccg gacaaatca gcaagggac ttttgcgtt cgagagaag gataatttg taccgatgc acagtttgt ggccgggtt tacacacac attagcaat ttttgccga cattacatg acgtcgcat aaactagac gtgaagatg ttctcgtgt ccggtgtgt tgcaaggat tttacgcgc aaagataac atgttggcg cacctgaaa atcatacac aagcagaat atgagctag
- >TC033029 RB
atgtcttcc caacgtttc tgtctgaga tggaacaac catcagtcg aatttgttg tcagtattc gaccaactc ctacacgac gaaagtttc gtcgatgtc acgttggcc gtggaagga caattgttg cgtgcccac aaaatggtc ctttcggcg tgcagtccc tacttccag gcgctgttc gtcaaccat cccgacaaa caccccatc gttatcctt aaggacgtc ccatacagc gatatgcgc tccttactg gacttcatg tacagaggg gaggtcagc gtcgaccag gacagactc acagccttc ctgcgggtc gctgaaagt ctgcggata aagggtctg acagaggtg aacgaagag aaatgtgat acaataacg tcgtctttg acgcaacaa aaccaaatg aacgccaac atgccacat ctgcaaaga ctgcaacag aacaagagg ttcggcaac ccgctcaac atgctcggc aatgctctt ctccagccg aagaggaag cgcggacgg cctcggaaa ctctcgggg agttcgaac ggcacccca ggcgaggac ttcgacagg ggcgatgcc ctggtgcag ggcagcccc gaaatgctc gaagtcaaa atgggaatg gacggattc acgggaaac aactccgac tgcagcacg ggcaagtcg gacgcgaag gaaaacgat ctggacgat aacaatacc aaagctaag gaagaaaac gaaccggtc gccggcacc tccaaggaa cagaacacg tttaacgac acgaaaaac gccgacgat tttcagtct tccgattcg ccgcaggcc ggcaagctg ctgagcccc aagcaggag ccgggcgag ggcgcgacg caggagccc cgcttgagc gccagcgac agcctgaac aacaacgcc ccagcgtcg agcggggcc cccatgtcg gcgccgacg tcgggacgc cagccggtg aagcgtcgg gcgcgccgc cgcgccacc tcccagagc caggacccc gccgagcag ctgacggag atgtccgtc cgggggctc aacctcttc cgctacgcc tccatcaac gagggcgtg tatcagtgc acggagtgc gccaaagtg gacgtccag aagacgttc aaaaacaag tacagcttc cagcggcac gcgttcctc taccacgaa ggacaccag cgcaaagtg tttccgtgc cccgtgtgc cagaaggag ttctctaga cccgacaaa atgaaaaat cacatgaag actgtacac gattgtttt atgcccaaa gacgtcgtg ttccctccg atggggttt tttccgata ccgtga
- >TC033029 RA
ggccccctc gcgtagtgc ccccttgtt gcccccaca cgcccagca caacgtcgc tgggccgct gcacgagcc ctcaatgta agagctcgc ctgcagtcg cccagccgc gtcttcggg acttcggag cgaggcgga gccgagtag tgcaatggt cgcttggcc atccacaat tcccaaggt cagcgcagc cgcgtggtg gtggtattc tgagccgga gctgcgcgg ggctcgagt tcaatttag ttagtcgaa gggggcgca cattgcacc ctcgcgtct ggcatggtg gtgtctcag tgcgagtga agtcaagga cacgctgtt gcgtaagta ggagcccct ttaaacggc agcgcgttt gaatttttc gcgcttttg cgaaccccg aactacccc gagtgccgc cgaacgacg gacatttgc tgacatggc gtcttgagt gttgtggtg ttcgctatc ccggggatc aacccttga ttttgtggt gacgcacga caagtgtta acaccaaca attgcgccc catatcaac aagtctgaa ttatccaca gtatcaatc cacaaagca tgtcttccc aacgtttct gtctgagat ggaacaacc atcagtcga atttgttgt cagtattcg accaactcc tacacgacg aaagtttcg tcgatgtca cgttggccg tggaaggac aattgttgc gtgcccaca aaatggtcc tttcggcgt gcagtccct acttccagg cgctgttcg tcaaccatc ccgacaaac accccatcg ttatcctta aggacgtcc catacagcg atatgcgct ccttactgg acttcatgt acagagggg aggtcagcg tcgaccagg acagactca cagccttcc tgcgggtcg ctgaaagtc tgcggataa agggtctga cagaggtga acgaagaga aatgtgata caataacgt cgtctttga cgcaacaaa accaaatga acgccaaca tgccacatc tgcaaagac tgcaacaga acaagaggt tcggcaacc cgctcaaca tgctcggca atgctcttc tccagccga agaggaagc gcggacggc ctcggaaac tctcgggga gttcgaacg gcaccccag gcgaggact tcgacaggg gcgatgccc tggtgcagg gcagccccg aaatgctcg aagtcaaaa tgggaatgg acggattca cgggaaaca actccgact gcagcacgg gcaagtcgg acgcgaagg aaaacgatc tggacgata acaatacca aagctaagg aagaaaacg aaccggtcg ccggcacct ccaaggaac agaacacgt ttaacgaca cgaaaaacg ccgacgatt ttcagtctt ccggcgaca aagaccgtt cctccccca acaatatcc actgtatag actttaact cgtcgctgc acgaagagt acaatttgc catatcagg acacctcca catccgaca tagagttag acgatttgg actcgaact atgagactc tcgatatta aactagatc caaagatgg tcctcgacg gcttctccg acaagaaga aacgcaaaa gtttgagtc ttatggacg tggaggact ttgccagct atgatatag tcctggacg acccctcct acgacttca aaaccctca aatacgtca attattcga aaaaaatca gcgacccgg acaaatcag caagggact tttgcgttc gagagaagg ataatttgt accgatgca cagtttgtg gccgggttt acacacaca ttagcaatt tttgccgac attacatga cgtcgcata aactagacg tgaagatgt tctcgtgtc cggtgtgtt gcaaggatt ttacgcgca aagataaca tgttggcgc acctgaaaa tcatacaca agcagaata tgagctagt gccttcgca agtgcctta ttacaagtc gatcggcaa taagtttcg ttattttta tactagcgt ttctagaga gtccaaata tttttataa tgatatata tgtgtgatt aattgggtt tatgggggt acaattcga aacgactga ttgcgacgc aaaattttt acgtgaaac ttgggtgga catcgaatt tgatctcaa atcgttact tagatttcg ccaatttta ccctccata aaaatgatt ataccaatg ttataaatc tatttagtt tagatattg ttagactat gttaagttg gcaataaat ttcctaaga gaatcctaa attagtctt gccttaatt gtttaatgc attttttta aaacgcaaa gtactgacg gaatcaaag gtaaagttg caatatttg tttttttat ggatgtttt tcatagcaa aattttgtt tttttttac tttatttct agtgtagat aatttattt tgcactttt atcgtttag ttacctttt tgtatgagt aaatgtaga tacccattt agttcagtt ggtttcttt tcaataaac attttattc gatt
- >TC033029 RB
ggccccctc gcgtagtgc ccccttgtt gcccccaca cgcccagca caacgtcgc tgggccgct gcacgagcc ctcaatgta agagctcgc ctgcagtcg cccagccgc gtcttcggg acttcggag cgaggcgga gccgagtag tgcaatggt cgcttggcc atccacaat tcccaaggt cagcgcagc cgcgtggtg gtggtattc tgagccgga gctgcgcgg ggctcgagt tcaatttag ttagtcgaa gggggcgca cattgcacc ctcgcgtct ggcatggtg gtgtctcag tgcgagtga agtcaagga cacgctgtt gctgttaac accaacaat tgcgcccca tatcaacaa gtctgaatt atccacagt atcaatcca caaagcatg tcttcccaa cgtttctgt ctgagatgg aacaaccat cagtcgaat ttgttgtca gtattcgac caactccta cacgacgaa agtttcgtc gatgtcacg ttggccgtg gaaggacaa ttgttgcgt gcccacaaa atggtcctt tcggcgtgc agtccctac ttccaggcg ctgttcgtc aaccatccc gacaaacac cccatcgtt atccttaag gacgtccca tacagcgat atgcgctcc ttactggac ttcatgtac agaggggag gtcagcgtc gaccaggac agactcaca gccttcctg cgggtcgct gaaagtctg cggataaag ggtctgaca gaggtgaac gaagagaaa tgtgataca ataacgtcg tctttgacg caacaaaac caaatgaac gccaacatg ccacatctg caaagactg caacagaac aagaggttc ggcaacccg ctcaacatg ctcggcaat gctcttctc cagccgaag aggaagcgc ggacggcct cggaaactc tcggggagt tcgaacggc accccaggc gaggacttc gacaggggc gatgccctg gtgcagggc agccccgaa atgctcgaa gtcaaaatg ggaatggac ggattcacg ggaaacaac tccgactgc agcacgggc aagtcggac gcgaaggaa aacgatctg gacgataac aataccaaa gctaaggaa gaaaacgaa ccggtcgcc ggcacctcc aaggaacag aacacgttt aacgacacg aaaaacgcc gacgatttt cagtcttcc gattcgccg caggccggc aagctgctg agccccaag caggagccg ggcgagggc gcgacgcag gagccccgc ttgagcgcc agcgacagc ctgaacaac aacgcccca gcgtcgagc ggggccccc atgtcggcg ccgacgtcg ggacgccag ccggtgaag cgtcgggcg cgccgccgc gccacctcc cagagccag gaccccgcc gagcagctg acggagatg tccgtccgg gggctcaac ctcttccgc tacgcctcc atcaacgag ggcgtgtat cagtgcacg gagtgcgcc aaagtggac gtccagaag acgttcaaa aacaagtac agcttccag cggcacgcg ttcctctac cacgaagga caccagcgc aaagtgttt ccgtgcccc gtgtgccag aaggagttc tctagaccc gacaaaatg aaaaatcac atgaagact gtacacgat tgttttatg cccaaagac gtcgtgttc cctccgatg gggtttttt ccgataccg tgaacgcgt tggtgacct tctgaagga cacgatccg gttgcgctg gaacgcaat taaaaaaat atgaattga tttcgtact tggtgctag gtttttgag aattttttt cgctaaatt caaagtaca atttatata agaacgaaa cgccgacga cgaaaatgc acagaagat accccggct tcacctgaa tgcccgatc agactggcc cttgatcag tgatcataa cttgaaaaa atcccaaat ttgaaaatt aagttggca atgccaact taaatcgta attgagatt gcatagcaa cggtgcaaa acaaacact gttattggc tgctgctat gcaactgaa acccgcgta atttgaacg ttcaatcta ggacgcgac agaatgtaa tgaaaaaaa atgcctatt tgataacgg taagtatgc gcaagataa acaaaaaat gcaggcaga taaagttta aattcttaa tagaaagag ttttgtaaa aacttcagt ctatatatt ttaatctca aatcacttg cagtcgtaa gccactctg atcggagtt ggtccaagg ctcggtatg attctgttg taagttttc gcgctgcgc tttcccaaa tagtactag ttagttatt cttgtgatt aacccgata cggttaaaa atggccttg taaaggccc tgtaattat tagaaataa tttaccaat tactgctaa gcagtttat aatccacaa ttatttttt aattcaata ttaacatcg tactagttt tttgtttgt ttctaaagt ttatcttag tttttttgg tttggatag attaggggt cttgcaaat attttagaa tagttgatt tacacaaaa tattttgtt cataacaaa ttttcgtct attataacc tttttacat gataagaat gtagaataa tgtttatac ataataatt taaactatc gactgcaca gagtagaca ataattata ttttatata atgccgtca gcgcacaaa cgtatcaag taagtaaac tatcaagta tttaattta ggtgttggg acattgaaa aacaatatt tttttaatt ctgtatttg ccatttttc gagaatctt atttatttc ttttctaag cttaattct ttccctttt ttagtatag gactaacaa aaacacctg aaacaaatt tattaacgt tttgtcaat tgtttaata gagctaatg gcaattcaa tcaaaataa gatatcaga tatcttaaa aaaacctaa cgcaatagc ttgtataat cacaatgtg atagtgttt tttgtttgt ttatttttt gtttttacg tttgttagg ccgaggcca gtttcgtca ctgttcttc catgtaaga ttgttactg ttgaaataa ttaaaaaac agtcacggt ctttgttag taagaataa taattacta agtgcaatt aatgtaaaa tttatatac taatgttaa atagattaa gtgaaaaaa gaaacagaa tatccgtat attttttgc aattattgc aacttgtta tcaaattga gaaaaatat ttattgaaa aattaaatt tttaaaaaa atttatata atgcacacc gcgtttcgt cgtcaatac aattgtttt tcccttctt gagcgattt atcattgtt tttgatctt tgtattaaa tgcttttag cctttgtat catattttg atttcttta tttgtgtta tttttgtta tagattatt ttttaactg tcactttgg tgcaaaatg tattatact atttgtgta attttactt cacaaaatc tatcataag aataattgt ttgtttatt gtactttca ttattaata aatctacct ctaaaca
