Status
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Gene information
General information
TC033029
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013842,TC013843
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3251167..3263933
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033029
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08211

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033029 RA
      MSSQRFCLR WNNHQSNLL SVFDQLLHD ESFVDVTLA 
      VEGQLLRAH KMVLSACSP YFQALFVNH PDKHPIVIL 
      KDVPYSDMR SLLDFMYRG EVSVDQDRL TAFLRVAES 
      LRIKGLTEV NEEKCDTIT SSLTQQNQM NANMPHLQR 
      LQQNKRFGN PLNMLGNAL LQPKRKRGR PRKLSGSSN 
      GTPGEDFDR GDALVQGSP EMLEVKMGM DGFTGNNSD 
      CSTGKSDAK ENDLDDNNT KAKEENEPV AGTSKEQNT 
      FNDTKNADD FQSSGDKDR SSPNNIHCI DFNSSLHEE 
      YNLPYQDTS TSDIELDDL DSNYETLDI KLDPKMVLD 
      GFSDKKKRK SLSLMDVED FASYDIVLD DPSYDFKTL 
      KYVNYSKKI SDPDKSARD FCVREKDNL YRCTVCGRV 
      YTHISNFCR HYMTSHKLD VKMFSCPVC CKDFTRKDN 
      MLAHLKIIH KQNMS
    • >TC033029 RB
      MSSQRFCLR WNNHQSNLL SVFDQLLHD ESFVDVTLA 
      VEGQLLRAH KMVLSACSP YFQALFVNH PDKHPIVIL 
      KDVPYSDMR SLLDFMYRG EVSVDQDRL TAFLRVAES 
      LRIKGLTEV NEEKCDTIT SSLTQQNQM NANMPHLQR 
      LQQNKRFGN PLNMLGNAL LQPKRKRGR PRKLSGSSN 
      GTPGEDFDR GDALVQGSP EMLEVKMGM DGFTGNNSD 
      CSTGKSDAK ENDLDDNNT KAKEENEPV AGTSKEQNT 
      FNDTKNADD FQSSDSPQA GKLLSPKQE PGEGATQEP 
      RLSASDSLN NNAPASSGA PMSAPTSGR QPVKRRARR 
      RATSQSQDP AEQLTEMSV RGLNLFRYA SINEGVYQC 
      TECAKVDVQ KTFKNKYSF QRHAFLYHE GHQRKVFPC 
      PVCQKEFSR PDKMKNHMK TVHDCFMPK DVVFPPMGF 
      FPIP
    CDS sequence
    • >TC033029 RA
      atgtcttcc caacgtttc tgtctgaga tggaacaac 
      catcagtcg aatttgttg tcagtattc gaccaactc 
      ctacacgac gaaagtttc gtcgatgtc acgttggcc 
      gtggaagga caattgttg cgtgcccac aaaatggtc 
      ctttcggcg tgcagtccc tacttccag gcgctgttc 
      gtcaaccat cccgacaaa caccccatc gttatcctt 
      aaggacgtc ccatacagc gatatgcgc tccttactg 
      gacttcatg tacagaggg gaggtcagc gtcgaccag 
      gacagactc acagccttc ctgcgggtc gctgaaagt 
      ctgcggata aagggtctg acagaggtg aacgaagag 
      aaatgtgat acaataacg tcgtctttg acgcaacaa 
      aaccaaatg aacgccaac atgccacat ctgcaaaga 
      ctgcaacag aacaagagg ttcggcaac ccgctcaac 
      atgctcggc aatgctctt ctccagccg aagaggaag 
      cgcggacgg cctcggaaa ctctcgggg agttcgaac 
      ggcacccca ggcgaggac ttcgacagg ggcgatgcc 
      ctggtgcag ggcagcccc gaaatgctc gaagtcaaa 
      atgggaatg gacggattc acgggaaac aactccgac 
      tgcagcacg ggcaagtcg gacgcgaag gaaaacgat 
      ctggacgat aacaatacc aaagctaag gaagaaaac 
      gaaccggtc gccggcacc tccaaggaa cagaacacg 
      tttaacgac acgaaaaac gccgacgat tttcagtct 
      tccggcgac aaagaccgt tcctccccc aacaatatc 
      cactgtata gactttaac tcgtcgctg cacgaagag 
      tacaatttg ccatatcag gacacctcc acatccgac 
      atagagtta gacgatttg gactcgaac tatgagact 
      ctcgatatt aaactagat ccaaagatg gtcctcgac 
      ggcttctcc gacaagaag aaacgcaaa agtttgagt 
      cttatggac gtggaggac tttgccagc tatgatata 
      gtcctggac gacccctcc tacgacttc aaaaccctc 
      aaatacgtc aattattcg aaaaaaatc agcgacccg 
      gacaaatca gcaagggac ttttgcgtt cgagagaag 
      gataatttg taccgatgc acagtttgt ggccgggtt 
      tacacacac attagcaat ttttgccga cattacatg 
      acgtcgcat aaactagac gtgaagatg ttctcgtgt 
      ccggtgtgt tgcaaggat tttacgcgc aaagataac 
      atgttggcg cacctgaaa atcatacac aagcagaat 
      atgagctag
    • >TC033029 RB
      atgtcttcc caacgtttc tgtctgaga tggaacaac 
      catcagtcg aatttgttg tcagtattc gaccaactc 
      ctacacgac gaaagtttc gtcgatgtc acgttggcc 
      gtggaagga caattgttg cgtgcccac aaaatggtc 
      ctttcggcg tgcagtccc tacttccag gcgctgttc 
      gtcaaccat cccgacaaa caccccatc gttatcctt 
      aaggacgtc ccatacagc gatatgcgc tccttactg 
      gacttcatg tacagaggg gaggtcagc gtcgaccag 
      gacagactc acagccttc ctgcgggtc gctgaaagt 
      ctgcggata aagggtctg acagaggtg aacgaagag 
      aaatgtgat acaataacg tcgtctttg acgcaacaa 
      aaccaaatg aacgccaac atgccacat ctgcaaaga 
      ctgcaacag aacaagagg ttcggcaac ccgctcaac 
      atgctcggc aatgctctt ctccagccg aagaggaag 
      cgcggacgg cctcggaaa ctctcgggg agttcgaac 
      ggcacccca ggcgaggac ttcgacagg ggcgatgcc 
      ctggtgcag ggcagcccc gaaatgctc gaagtcaaa 
      atgggaatg gacggattc acgggaaac aactccgac 
      tgcagcacg ggcaagtcg gacgcgaag gaaaacgat 
      ctggacgat aacaatacc aaagctaag gaagaaaac 
      gaaccggtc gccggcacc tccaaggaa cagaacacg 
      tttaacgac acgaaaaac gccgacgat tttcagtct 
      tccgattcg ccgcaggcc ggcaagctg ctgagcccc 
      aagcaggag ccgggcgag ggcgcgacg caggagccc 
      cgcttgagc gccagcgac agcctgaac aacaacgcc 
      ccagcgtcg agcggggcc cccatgtcg gcgccgacg 
      tcgggacgc cagccggtg aagcgtcgg gcgcgccgc 
      cgcgccacc tcccagagc caggacccc gccgagcag 
      ctgacggag atgtccgtc cgggggctc aacctcttc 
      cgctacgcc tccatcaac gagggcgtg tatcagtgc 
      acggagtgc gccaaagtg gacgtccag aagacgttc 
      aaaaacaag tacagcttc cagcggcac gcgttcctc 
      taccacgaa ggacaccag cgcaaagtg tttccgtgc 
      cccgtgtgc cagaaggag ttctctaga cccgacaaa 
      atgaaaaat cacatgaag actgtacac gattgtttt 
      atgcccaaa gacgtcgtg ttccctccg atggggttt 
      tttccgata ccgtga
    mRNA sequence
    • >TC033029 RA
      ggccccctc gcgtagtgc ccccttgtt gcccccaca 
      cgcccagca caacgtcgc tgggccgct gcacgagcc 
      ctcaatgta agagctcgc ctgcagtcg cccagccgc 
      gtcttcggg acttcggag cgaggcgga gccgagtag 
      tgcaatggt cgcttggcc atccacaat tcccaaggt 
      cagcgcagc cgcgtggtg gtggtattc tgagccgga 
      gctgcgcgg ggctcgagt tcaatttag ttagtcgaa 
      gggggcgca cattgcacc ctcgcgtct ggcatggtg 
      gtgtctcag tgcgagtga agtcaagga cacgctgtt 
      gcgtaagta ggagcccct ttaaacggc agcgcgttt 
      gaatttttc gcgcttttg cgaaccccg aactacccc 
      gagtgccgc cgaacgacg gacatttgc tgacatggc 
      gtcttgagt gttgtggtg ttcgctatc ccggggatc 
      aacccttga ttttgtggt gacgcacga caagtgtta 
      acaccaaca attgcgccc catatcaac aagtctgaa 
      ttatccaca gtatcaatc cacaaagca tgtcttccc 
      aacgtttct gtctgagat ggaacaacc atcagtcga 
      atttgttgt cagtattcg accaactcc tacacgacg 
      aaagtttcg tcgatgtca cgttggccg tggaaggac 
      aattgttgc gtgcccaca aaatggtcc tttcggcgt 
      gcagtccct acttccagg cgctgttcg tcaaccatc 
      ccgacaaac accccatcg ttatcctta aggacgtcc 
      catacagcg atatgcgct ccttactgg acttcatgt 
      acagagggg aggtcagcg tcgaccagg acagactca 
      cagccttcc tgcgggtcg ctgaaagtc tgcggataa 
      agggtctga cagaggtga acgaagaga aatgtgata 
      caataacgt cgtctttga cgcaacaaa accaaatga 
      acgccaaca tgccacatc tgcaaagac tgcaacaga 
      acaagaggt tcggcaacc cgctcaaca tgctcggca 
      atgctcttc tccagccga agaggaagc gcggacggc 
      ctcggaaac tctcgggga gttcgaacg gcaccccag 
      gcgaggact tcgacaggg gcgatgccc tggtgcagg 
      gcagccccg aaatgctcg aagtcaaaa tgggaatgg 
      acggattca cgggaaaca actccgact gcagcacgg 
      gcaagtcgg acgcgaagg aaaacgatc tggacgata 
      acaatacca aagctaagg aagaaaacg aaccggtcg 
      ccggcacct ccaaggaac agaacacgt ttaacgaca 
      cgaaaaacg ccgacgatt ttcagtctt ccggcgaca 
      aagaccgtt cctccccca acaatatcc actgtatag 
      actttaact cgtcgctgc acgaagagt acaatttgc 
      catatcagg acacctcca catccgaca tagagttag 
      acgatttgg actcgaact atgagactc tcgatatta 
      aactagatc caaagatgg tcctcgacg gcttctccg 
      acaagaaga aacgcaaaa gtttgagtc ttatggacg 
      tggaggact ttgccagct atgatatag tcctggacg 
      acccctcct acgacttca aaaccctca aatacgtca 
      attattcga aaaaaatca gcgacccgg acaaatcag 
      caagggact tttgcgttc gagagaagg ataatttgt 
      accgatgca cagtttgtg gccgggttt acacacaca 
      ttagcaatt tttgccgac attacatga cgtcgcata 
      aactagacg tgaagatgt tctcgtgtc cggtgtgtt 
      gcaaggatt ttacgcgca aagataaca tgttggcgc 
      acctgaaaa tcatacaca agcagaata tgagctagt 
      gccttcgca agtgcctta ttacaagtc gatcggcaa 
      taagtttcg ttattttta tactagcgt ttctagaga 
      gtccaaata tttttataa tgatatata tgtgtgatt 
      aattgggtt tatgggggt acaattcga aacgactga 
      ttgcgacgc aaaattttt acgtgaaac ttgggtgga 
      catcgaatt tgatctcaa atcgttact tagatttcg 
      ccaatttta ccctccata aaaatgatt ataccaatg 
      ttataaatc tatttagtt tagatattg ttagactat 
      gttaagttg gcaataaat ttcctaaga gaatcctaa 
      attagtctt gccttaatt gtttaatgc attttttta 
      aaacgcaaa gtactgacg gaatcaaag gtaaagttg 
      caatatttg tttttttat ggatgtttt tcatagcaa 
      aattttgtt tttttttac tttatttct agtgtagat 
      aatttattt tgcactttt atcgtttag ttacctttt 
      tgtatgagt aaatgtaga tacccattt agttcagtt 
      ggtttcttt tcaataaac attttattc gatt
    • >TC033029 RB
      ggccccctc gcgtagtgc ccccttgtt gcccccaca 
      cgcccagca caacgtcgc tgggccgct gcacgagcc 
      ctcaatgta agagctcgc ctgcagtcg cccagccgc 
      gtcttcggg acttcggag cgaggcgga gccgagtag 
      tgcaatggt cgcttggcc atccacaat tcccaaggt 
      cagcgcagc cgcgtggtg gtggtattc tgagccgga 
      gctgcgcgg ggctcgagt tcaatttag ttagtcgaa 
      gggggcgca cattgcacc ctcgcgtct ggcatggtg 
      gtgtctcag tgcgagtga agtcaagga cacgctgtt 
      gctgttaac accaacaat tgcgcccca tatcaacaa 
      gtctgaatt atccacagt atcaatcca caaagcatg 
      tcttcccaa cgtttctgt ctgagatgg aacaaccat 
      cagtcgaat ttgttgtca gtattcgac caactccta 
      cacgacgaa agtttcgtc gatgtcacg ttggccgtg 
      gaaggacaa ttgttgcgt gcccacaaa atggtcctt 
      tcggcgtgc agtccctac ttccaggcg ctgttcgtc 
      aaccatccc gacaaacac cccatcgtt atccttaag 
      gacgtccca tacagcgat atgcgctcc ttactggac 
      ttcatgtac agaggggag gtcagcgtc gaccaggac 
      agactcaca gccttcctg cgggtcgct gaaagtctg 
      cggataaag ggtctgaca gaggtgaac gaagagaaa 
      tgtgataca ataacgtcg tctttgacg caacaaaac 
      caaatgaac gccaacatg ccacatctg caaagactg 
      caacagaac aagaggttc ggcaacccg ctcaacatg 
      ctcggcaat gctcttctc cagccgaag aggaagcgc 
      ggacggcct cggaaactc tcggggagt tcgaacggc 
      accccaggc gaggacttc gacaggggc gatgccctg 
      gtgcagggc agccccgaa atgctcgaa gtcaaaatg 
      ggaatggac ggattcacg ggaaacaac tccgactgc 
      agcacgggc aagtcggac gcgaaggaa aacgatctg 
      gacgataac aataccaaa gctaaggaa gaaaacgaa 
      ccggtcgcc ggcacctcc aaggaacag aacacgttt 
      aacgacacg aaaaacgcc gacgatttt cagtcttcc 
      gattcgccg caggccggc aagctgctg agccccaag 
      caggagccg ggcgagggc gcgacgcag gagccccgc 
      ttgagcgcc agcgacagc ctgaacaac aacgcccca 
      gcgtcgagc ggggccccc atgtcggcg ccgacgtcg 
      ggacgccag ccggtgaag cgtcgggcg cgccgccgc 
      gccacctcc cagagccag gaccccgcc gagcagctg 
      acggagatg tccgtccgg gggctcaac ctcttccgc 
      tacgcctcc atcaacgag ggcgtgtat cagtgcacg 
      gagtgcgcc aaagtggac gtccagaag acgttcaaa 
      aacaagtac agcttccag cggcacgcg ttcctctac 
      cacgaagga caccagcgc aaagtgttt ccgtgcccc 
      gtgtgccag aaggagttc tctagaccc gacaaaatg 
      aaaaatcac atgaagact gtacacgat tgttttatg 
      cccaaagac gtcgtgttc cctccgatg gggtttttt 
      ccgataccg tgaacgcgt tggtgacct tctgaagga 
      cacgatccg gttgcgctg gaacgcaat taaaaaaat 
      atgaattga tttcgtact tggtgctag gtttttgag 
      aattttttt cgctaaatt caaagtaca atttatata 
      agaacgaaa cgccgacga cgaaaatgc acagaagat 
      accccggct tcacctgaa tgcccgatc agactggcc 
      cttgatcag tgatcataa cttgaaaaa atcccaaat 
      ttgaaaatt aagttggca atgccaact taaatcgta 
      attgagatt gcatagcaa cggtgcaaa acaaacact 
      gttattggc tgctgctat gcaactgaa acccgcgta 
      atttgaacg ttcaatcta ggacgcgac agaatgtaa 
      tgaaaaaaa atgcctatt tgataacgg taagtatgc 
      gcaagataa acaaaaaat gcaggcaga taaagttta 
      aattcttaa tagaaagag ttttgtaaa aacttcagt 
      ctatatatt ttaatctca aatcacttg cagtcgtaa 
      gccactctg atcggagtt ggtccaagg ctcggtatg 
      attctgttg taagttttc gcgctgcgc tttcccaaa 
      tagtactag ttagttatt cttgtgatt aacccgata 
      cggttaaaa atggccttg taaaggccc tgtaattat 
      tagaaataa tttaccaat tactgctaa gcagtttat 
      aatccacaa ttatttttt aattcaata ttaacatcg 
      tactagttt tttgtttgt ttctaaagt ttatcttag 
      tttttttgg tttggatag attaggggt cttgcaaat 
      attttagaa tagttgatt tacacaaaa tattttgtt 
      cataacaaa ttttcgtct attataacc tttttacat 
      gataagaat gtagaataa tgtttatac ataataatt 
      taaactatc gactgcaca gagtagaca ataattata 
      ttttatata atgccgtca gcgcacaaa cgtatcaag 
      taagtaaac tatcaagta tttaattta ggtgttggg 
      acattgaaa aacaatatt tttttaatt ctgtatttg 
      ccatttttc gagaatctt atttatttc ttttctaag 
      cttaattct ttccctttt ttagtatag gactaacaa 
      aaacacctg aaacaaatt tattaacgt tttgtcaat 
      tgtttaata gagctaatg gcaattcaa tcaaaataa 
      gatatcaga tatcttaaa aaaacctaa cgcaatagc 
      ttgtataat cacaatgtg atagtgttt tttgtttgt 
      ttatttttt gtttttacg tttgttagg ccgaggcca 
      gtttcgtca ctgttcttc catgtaaga ttgttactg 
      ttgaaataa ttaaaaaac agtcacggt ctttgttag 
      taagaataa taattacta agtgcaatt aatgtaaaa 
      tttatatac taatgttaa atagattaa gtgaaaaaa 
      gaaacagaa tatccgtat attttttgc aattattgc 
      aacttgtta tcaaattga gaaaaatat ttattgaaa 
      aattaaatt tttaaaaaa atttatata atgcacacc 
      gcgtttcgt cgtcaatac aattgtttt tcccttctt 
      gagcgattt atcattgtt tttgatctt tgtattaaa 
      tgcttttag cctttgtat catattttg atttcttta 
      tttgtgtta tttttgtta tagattatt ttttaactg 
      tcactttgg tgcaaaatg tattatact atttgtgta 
      attttactt cacaaaatc tatcataag aataattgt 
      ttgtttatt gtactttca ttattaata aatctacct 
      ctaaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY