Status
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Gene information
General information
TC013937 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2608969..2611043
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013937
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08209

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Adult morphology
    low validity due to technical reasons - should be repeated: ovary phenotype not scorable,
    only 1 of 4 ovaries scorable due to bad and difficult preparation
    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    Comment: based on observations during preparation
    vitellarium irregular - Number of animals showing the phenotype: 1

    tropharium without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    remarks - cuticles: bad quality of preparation,
    ovary fragile. ovarioles rupturing

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013937 RA
      MGKKSKSSS GLGRSLIKD RFGSTKGRK LTADKSMLH 
      TTELQDGYD WGRLNLQSV TEESSFQEF LATAELTGA 
      EFQAEKLDI KFVNPKSNV GLLTKDEMN KAKDLHDKH 
      KGELRIPRR PPWNANMSK EELDQNEKE YFLQWRKGL 
      AKLQEDEGI LLTPYEKNL EFWRQLWRV VEKSDVIVQ 
      IVDARNPLL FYCEDLEKY TKEVSPDKM NIVLVNKAD 
      FLTLEQRQA WADYFMSQN VKAVFYSAA LASETPKES 
      EPRGDEDQS KLNQLQDCV SNLEKEVEK TAQKLNEII 
      GALKLEFQN SSEILTKDQ LMDLFKDIH KGPKVTEDI 
      TTIGLVGYP NVGKSSTIN SLLSAKKVS VSATPGKTK 
      HFQTLFLTK NLLLCDCPG LVMPKFVFT KAEMIINGI 
      LPIDQMRDH VPPVNLIAT LIPKHVLED KYGIMLPAP 
      LEGEDPDRC PTSEELLNA YAYNRGFMT ANGQPDNPR 
      AARYILKDY MNGKLLYCH APPNVVQRE YHRWPERQK 
      FRSLEKSAP PRQIRAIKT SRATTEDVD KLFFHGGSS 
      TVHTKGKTV INGYGAANG SVQQLGDKP WKKINRHEN 
      KNKREKLRR VYAHLDQH
    CDS sequence
    • >TC013937 RA
      atgggtaaa aaatcaaaa tcttcgtct ggtttgggc 
      cgttccttg atcaaggac cgcttcggg agcaccaaa 
      ggtcgtaaa ctaacagcg gacaagtcc atgttgcat 
      acgacggaa ttgcaagat gggtacgat tggggccgt 
      ttgaattta caatcagtg accgaggag tcatcgttt 
      caagagttc ttggccacc gctgaatta acaggtgcc 
      gagtttcag gctgaaaag cttgacatc aagttcgtg 
      aaccctaaa tcaaatgtg ggtttgctg actaaagat 
      gagatgaat aaagcgaag gatttgcat gacaagcac 
      aagggggag ttgcggatt ccgagacgg cccccttgg 
      aacgcaaac atgagtaaa gaagagctg gatcaaaat 
      gaaaaagag tactttctc cagtggcgc aaaggcttg 
      gccaaattg caggaggac gaaggcatt ctcttgact 
      ccttatgag aagaatctt gagttttgg agacaattg 
      tggagagtt gtggagaaa agtgacgtg attgtgcaa 
      attgttgat gccagaaac ccgcttctt ttctattgt 
      gaagacttg gaaaaatac acaaaggaa gtgtcacca 
      gacaaaatg aacattgtc ctagttaac aaagccgat 
      tttttaaca ctggagcaa aggcaagcc tgggctgac 
      tatttcatg tcgcaaaac gtgaaagct gttttttat 
      tcagcggct ttagcttcg gaaacccca aaagagagt 
      gaaccgaga ggtgacgag gaccaatca aaactgaat 
      cagctgcaa gactgcgta tccaacttg gaaaaagaa 
      gttgaaaaa actgcccaa aaattgaat gagattatt 
      ggggcccta aaactagag tttcaaaat tcaagcgag 
      atattaacc aaagatcag ttgatggat ttgttcaaa 
      gacattcat aaggggcct aaagttact gaagatatc 
      actactatt ggccttgtt gggtatcca aatgtgggg 
      aaaagttct acaattaat tcactgttg tctgctaag 
      aaagtttca gtttcggca actccggga aaaaccaaa 
      catttccaa acgcttttt cttacgaaa aatttgctt 
      ctctgcgat tgtcccggc ctggtaatg cctaagttt 
      gttttcaca aaagccgaa atgattata aatggaatc 
      ctgccaata gatcaaatg cgtgatcat gtgccccct 
      gtaaattta attgcgacg ttaattccc aaacatgtg 
      ttagaagac aagtatggg attatgtta ccagctccc 
      ttggagggc gaggaccct gacaggtgt cccacttca 
      gaggaatta ctcaatgca tatgcttat aacagaggg 
      tttatgact gctaatggc caacctgat aatcccaga 
      gccgctaga tacattttg aaggattat atgaatggt 
      aaattatta tattgtcac gctcctcca aatgttgtc 
      caaagggag taccacagg tggccagaa aggcagaaa 
      tttcgctct ttggaaaaa tcagcccct cctcgccaa 
      atacgtgct ataaaaaca agtcgtgca acaactgaa 
      gacgttgat aagttattt tttcacgga ggctcatca 
      actgtacat acaaaagga aagacagtg ataaatggt 
      tatggggct gccaatggc agcgtccaa caactgggt 
      gataaacct tggaaaaaa atcaacagg catgaaaat 
      aaaaacaag agggagaaa ttgagaaga gtttatgct 
      catctggac caacactga
    mRNA sequence
    • >TC013937 RA
      tatgttgaa aagagacgg gtggtcgac acctgacag 
      ttgaggtta tgtctcctc gtttttcat tttttgact 
      gaaaaaaac atgggtaaa aaatcaaaa tcttcgtct 
      ggtttgggc cgttccttg atcaaggac cgcttcggg 
      agcaccaaa ggtcgtaaa ctaacagcg gacaagtcc 
      atgttgcat acgacggaa ttgcaagat gggtacgat 
      tggggccgt ttgaattta caatcagtg accgaggag 
      tcatcgttt caagagttc ttggccacc gctgaatta 
      acaggtgcc gagtttcag gctgaaaag cttgacatc 
      aagttcgtg aaccctaaa tcaaatgtg ggtttgctg 
      actaaagat gagatgaat aaagcgaag gatttgcat 
      gacaagcac aagggggag ttgcggatt ccgagacgg 
      cccccttgg aacgcaaac atgagtaaa gaagagctg 
      gatcaaaat gaaaaagag tactttctc cagtggcgc 
      aaaggcttg gccaaattg caggaggac gaaggcatt 
      ctcttgact ccttatgag aagaatctt gagttttgg 
      agacaattg tggagagtt gtggagaaa agtgacgtg 
      attgtgcaa attgttgat gccagaaac ccgcttctt 
      ttctattgt gaagacttg gaaaaatac acaaaggaa 
      gtgtcacca gacaaaatg aacattgtc ctagttaac 
      aaagccgat tttttaaca ctggagcaa aggcaagcc 
      tgggctgac tatttcatg tcgcaaaac gtgaaagct 
      gttttttat tcagcggct ttagcttcg gaaacccca 
      aaagagagt gaaccgaga ggtgacgag gaccaatca 
      aaactgaat cagctgcaa gactgcgta tccaacttg 
      gaaaaagaa gttgaaaaa actgcccaa aaattgaat 
      gagattatt ggggcccta aaactagag tttcaaaat 
      tcaagcgag atattaacc aaagatcag ttgatggat 
      ttgttcaaa gacattcat aaggggcct aaagttact 
      gaagatatc actactatt ggccttgtt gggtatcca 
      aatgtgggg aaaagttct acaattaat tcactgttg 
      tctgctaag aaagtttca gtttcggca actccggga 
      aaaaccaaa catttccaa acgcttttt cttacgaaa 
      aatttgctt ctctgcgat tgtcccggc ctggtaatg 
      cctaagttt gttttcaca aaagccgaa atgattata 
      aatggaatc ctgccaata gatcaaatg cgtgatcat 
      gtgccccct gtaaattta attgcgacg ttaattccc 
      aaacatgtg ttagaagac aagtatggg attatgtta 
      ccagctccc ttggagggc gaggaccct gacaggtgt 
      cccacttca gaggaatta ctcaatgca tatgcttat 
      aacagaggg tttatgact gctaatggc caacctgat 
      aatcccaga gccgctaga tacattttg aaggattat 
      atgaatggt aaattatta tattgtcac gctcctcca 
      aatgttgtc caaagggag taccacagg tggccagaa 
      aggcagaaa tttcgctct ttggaaaaa tcagcccct 
      cctcgccaa atacgtgct ataaaaaca agtcgtgca 
      acaactgaa gacgttgat aagttattt tttcacgga 
      ggctcatca actgtacat acaaaagga aagacagtg 
      ataaatggt tatggggct gccaatggc agcgtccaa 
      caactgggt gataaacct tggaaaaaa atcaacagg 
      catgaaaat aaaaacaag agggagaaa ttgagaaga 
      gtttatgct catctggac caacactga cgtctctgt 
      tatgtaaaa gt
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