| General information | |||
| TC033023
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 2573359..2584673 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033023 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08208 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033023 RA
MAFKMSYSF EGNYRTKPE QNLSGASKK ISRDVLLQK THEERRKRQ EQRLKLQSA QLLQSHIRS YLVRKHKKE EERAYYDNN FENIPIQVL LSKLLFFYD PVKDNMRLY QISERLLQQ NVQINQALG SDTALSWLI KWWLVLCLK SSYHPDMTK LMLNCLDAF VCNTPTIEY LTRKGYYVY LRTLLDNDA KELLQQILS LIYRPFQSL RITQSSQDI ILSEFCSNF LKPTFTNNV KTVLIPYLK NKRDFPYEK IIRYLNSGY HFQISNSLF YCILALEPD DYEPTSDSI QVLSTLSVD IHQLKPPVY LISDDSDDE SEVQVSEEE LLLSDYLHI MNKPERVRK WLHFFEQNS NNEDVLMSL TKFCHNLLL VYKDSIRKF LLLYKLGLN GNFLNKLWN MLNRKRSLE GELRGSCLL SWKECHTTL SVFCDTFTF YTETLTDRE NSDTSETFT QNDLYSMSK LLKNVAVDL VELAFPMCR SSTIPATPE ILHLYSSCL NCVKMLYML DIRKQFCPH GFWTAKKIH ISQDLSRRN YLSKTIRPF HGLVCDMDD EEHLPPLST IEQRSLAVL QELPFLIGF NTRVLLLRD WCRNSLGEN DYQRLHNEF LNDNLVVIR RTHLYEDAF EKLSVKNES DLRHKVRIQ FINSVGLEE AGIDGGGIF KEFINEVLK TAFDPNRGF FLLTADNTL YPNPNVHLI VENFMEHYY FIGRLVGKA IFENILVDL PLAEFFLAK LLVDRASAH YLKSLDPVL YRNLLYLRD YPGDVSDLG LDFTTVNND LGETRVVEL KPHGSNIQV TNENRLEYI QRLADLKLN VQLKKQCMA FREGLDSVV PLLWLKLFN HNELQVIIG GDTQEIDLS DLKAHTVYG GEFTADHPT VNLFWKILN TFTDTQKKM LLKFVTSCS RPPLLGFKE LNPQFCIQS SGSEDRMPT ASTCLNLLK IPIIKEEEV LRNKLLQAI EQQAGFELS ITTMDFDDM LEELGELGK YQITTYLLV CLPVFFAAA NSLSYVFTA GIPNYRCFI PECEDAENA TYEVPWIDF AIPGGDSRE ILDSKSHLC DRFTVNETV ANYTYCSRD LFTNVTETC NEWVFDSEE RTIVNDWNL TCVENQWKL SFVGTSHFA GIIVGSGLF GILADRYGR KRIFIICIL IMSVSGCAQ VVSPEYITF VILMFVNAM GTAGVYPLA FIIGVELVG KRKREITGI VLNYFYAVG EAVVALVAW YTKDWVLLQ LIVSSPSIL FFIYYWFIP ESVRWLLAN EKREKAKGI VCKAAKVNN VTLSESLLA SFKKDAASN SDSGQDEGT KILFVLKTI LKSRKLVVR MLITYFIWS VNAFVFYGL SVNSTSMSG NKYINFALV SLVEIPGYT IAWVSIQKI GRRLSLVGS LLLCGLTCT LTIFVPSEM TWAVILLFL IGKLGITAA FGVVYVHTA EMLPTVVRS GGVGSASTM ARFGALLAP FVPLLGIYV KPLPMLLFG GVAVLAGIL ALKLPETLG NKLPESVEE AENL
- >TC033023 RA
atggcgttt aaaatgtca tatagtttt gaaggcaac tacaggaca aagcctgag caaaatttg tcaggcgct agtaaaaaa atttcgagg gatgttttg ctacaaaaa acccacgaa gagagacgt aaacgacag gagcaacgt ctgaagtta caaagtgct caacttttg cagtcacac attaggagt tatttagtg cgcaaacat aaaaaggag gaggaaagg gcgtactat gataacaat tttgaaaat atcccgata caggtgttg ttgtccaaa cttctgttc ttttatgac ccggttaag gacaacatg cggctgtac caaatatcc gaacgattg ttgcaacaa aatgtgcaa atcaatcaa gcgcttggc tcagatacg gccctttcg tggctcatc aaatggtgg ctggttttg tgtctcaaa agctcgtat caccctgat atgacaaag ctcatgtta aattgcctt gacgcgttc gtttgcaac acaccgaca attgaatat ctcacacgc aaaggttac tacgtgtac ctgcgtact ctcctggat aatgacgct aaagagtta cttcaacaa atcctaagt ctgatatat cgccccttc caatcgcta cgaatcacc cagagttcg caggacatc attttgtcc gaattctgc agcaatttc ctcaaaccc accttcaca aacaacgta aagactgtc cttataccg tatttaaaa aacaaacgc gattttcca tacgaaaaa atcataagg tacttgaat agtggctac cacttccaa atcagcaat agtttgttt tactgcatt ttggcgctt gaacccgac gattacgaa cccacaagt gatagtatt caagtcttg tccacactt agcgtggac atccaccag ttgaaaccc ccggtgtat ttaataagc gacgatagt gacgatgag agcgaagtc caggtctcg gaagaagaa ttactcctc tctgactat ttacatatc atgaataag ccggaacgg gtccggaaa tggttgcac tttttcgaa caaaatagc aataatgaa gatgtgcta atgtcgctg acgaagttt tgccacaat ctcctactt gtgtacaag gattcgatt aggaaattt ttattgttg tacaaattg gggttgaac ggcaatttc ctcaacaaa ttatggaat atgttgaac cggaagagg tcgctagag ggcgaactc cgggggtct tgcctcctg tcgtggaaa gagtgtcac acgaccctc tcggtattt tgcgacact tttactttc tacacggaa accttaacc gatcgggaa aattccgat actagtgag actttcacg caaaatgat ctctactca atgtcgaag ttactgaaa aacgttgcg gtggattta gtcgaattg gcgtttccg atgtgcagg tcaagcacg attccagcg actccggaa attctgcat ctgtacagt tcgtgtctg aactgtgtt aaaatgttg tacatgttg gatattcgc aaacagttt tgtccgcat gggttctgg acggcgaaa aaaatccac atttcgcag gatttgtca cgaaggaat tatttgtcg aagacgatc aggccgttc catggcttg gtgtgtgat atggacgat gaggaacac ttgccccca ttgtcaacc atcgagcaa cgttccttg gcagttttg caggagttg ccgtttttg atcgggttt aatacaagg gttttgctt ttgagggat tggtgcagg aacagtttg ggcgaaaat gattaccag cgccttcat aatgaattt ctcaatgat aatctcgtg gtgataagg cggacgcat ttgtacgaa gatgcgttt gaaaaattg tcagttaag aatgaatcc gatctgagg cacaaggtg cgaattcag tttataaac agtgtgggt ttggaggaa gctgggatt gacggtggt gggattttt aaggagttt attaatgaa gttctgaaa actgcattt gaccccaac cggggattt ttcctctta acggctgac aacacactg tatccaaac ccaaatgtc cacttgatt gtggaaaat ttcatggaa cactactat ttcatcggc cgattagtc ggaaaagca attttcgaa aacatacta gtggatcta cctttagcc gaatttttc ctagccaaa ttattagta gaccgagcc agtgctcac tacttaaaa tcactggac ccggtcctc tatcgaaat ctcttgtat ttgagggat tacccgggc gatgtgagc gacctagga ttggatttt acgacagtt aataacgac ttgggcgag actagagtc gttgagctt aaacctcac gggagcaac atccaggta acaaacgag aatcgactc gagtacatt caacgattg gcggatttg aaattaaac gttcaattg aaaaaacaa tgtatggca tttcgggag ggtcttgac agtgtcgtg cccttgctg tggttaaaa ctcttcaac cataacgag ctgcaagtc atcataggg ggcgatacg caggaaatt gatttgagt gacttgaaa gctcacact gtatatgga ggcgaattt acggccgat caccccact gtgaatctg ttctggaaa attttaaac acgttcacc gacactcag aaaaaaatg ttgctcaag tttgtaacg agttgttca aggccgcca ctgctgggc tttaaagaa cttaatccc cagttttgc attcaatcg tcagggagt gaggatcgg atgccgacc gcaagcacg tgcttgaat ttactcaaa ataccaatc attaaggag gaagaagtt ttaagaaat aaactgctg caagcgatt gaacaacag gctggtttt gagttatcg attaccacc atggacttt gacgatatg ttagaagaa ttaggcgag ttggggaaa taccaaatc acaacgtac cttttagtc tgtcttccc gtatttttc gcagcggcc aatagtctc agttacgtg tttacggca ggaatacca aattacagg tgttttatc cccgagtgc gaagatgca gaaaatgca acttacgaa gtcccttgg atcgacttt gccatacct ggaggcgac tctagggaa atattggat tcgaaatcc cacctctgt gataggttt accgtaaat gaaacagtc gcgaattac acttattgt tcgagagat ttgtttaca aatgtgact gaaacgtgc aacgaatgg gtgtttgat agcgaggag cggacaatc gtaaatgat tggaacttg acttgtgtg gaaaatcaa tggaaactc tcctttgtg ggcacgagt catttcgcg gggattatc gtgggatcg ggattattt gggattttg gccgacagg tatggacgc aaaagaata tttatcatc tgtatcctg atcatgagc gtgtcagga tgtgcccaa gttgtctca cccgaatac ataactttc gtgatatta atgtttgtg aatgcgatg ggcacggct ggagtctac cctttggct ttcattatc ggggtcgaa ttggttggc aaaaggaag cgagaaata accggaatc gtattaaac tatttctac gctgttggt gaggctgtt gttgccctt gtcgcgtgg tacaccaaa gactgggtt ttattacaa ctcatagtt tctagtccg tctatttta tttttcata tattactgg tttatacca gaatccgtg cggtggctg ctagccaac gaaaaacgc gaaaaggcc aaaggcatc gtttgcaaa gcggcgaaa gtaaacaac gtcacttta tccgagtct ttactggcc tcattcaag aaagacgca gcctcgaat agcgacagt ggccaagat gaaggaaca aaaatttta ttcgtattg aaaacgatc ctaaaatcg cgtaaactc gtggtcaga atgctgatc acgtatttc atttggtct gtgaacgca tttgtattc tacggactg tcggtgaat tcgaccagc atgagcggg aacaaatac atcaatttt gcgttagtg agcttggtt gaaattccc ggttataca attgcttgg gtcagtatt caaaaaatc gggcgccgg ttgtccctt gttgggtct ttacttttg tgtggtttg acttgtact ttgactatt ttcgtacct tccgaaatg acttgggcg gttattctg ctatttctc attgggaaa ctggggatc acagctgcg tttggtgtc gtttacgtc cacacagcc gaaatgttg ccaacagtg gtgcggagc gggggagtg ggatctgcg tcgactatg gcccgattt ggggcgctt ttagccccc tttgtccct cttctggga atttatgtc aaaccgttg ccgatgcta ctgttcggg ggggttgca gtgctggcg ggaatcttg gcgttgaag ttacctgaa actttggga aataaatta cctgaaagt gtagaagag gctgaaaat ttgtaa
- >TC033023 RA
aaattccat tattttgta atttgtttt tgtacaaaa agcgaccaa aattttgca aaatggcgt ttaaaatgt catatagtt ttgaaggca actacagga caaagcctg agcaaaatt tgtcaggcg ctagtaaaa aaatttcga gggatgttt tgctacaaa aaacccacg aagagagac gtaaacgac aggagcaac gtctgaagt tacaaagtg ctcaacttt tgcagtcac acattagga gttatttag tgcgcaaac ataaaaagg aggaggaaa gggcgtact atgataaca attttgaaa atatcccga tacaggtgt tgttgtcca aacttctgt tcttttatg acccggtta aggacaaca tgcggctgt accaaatat ccgaacgat tgttgcaac aaaatgtgc aaatcaatc aagcgcttg gctcagata cggcccttt cgtggctca tcaaatggt ggctggttt tgtgtctca aaagctcgt atcaccctg atatgacaa agctcatgt taaattgcc ttgacgcgt tcgtttgca acacaccga caattgaat atctcacac gcaaaggtt actacgtgt acctgcgta ctctcctgg ataatgacg ctaaagagt tacttcaac aaatcctaa gtctgatat atcgcccct tccaatcgc tacgaatca cccagagtt cgcaggaca tcattttgt ccgaattct gcagcaatt tcctcaaac ccaccttca caaacaacg taaagactg tccttatac cgtatttaa aaaacaaac gcgattttc catacgaaa aaatcataa ggtacttga atagtggct accacttcc aaatcagca atagtttgt tttactgca ttttggcgc ttgaacccg acgattacg aacccacaa gtgatagta ttcaagtct tgtccacac ttagcgtgg acatccacc agttgaaac ccccggtgt atttaataa gcgacgata gtgacgatg agagcgaag tccaggtct cggaagaag aattactcc tctctgact atttacata tcatgaata agccggaac gggtccgga aatggttgc actttttcg aacaaaata gcaataatg aagatgtgc taatgtcgc tgacgaagt tttgccaca atctcctac ttgtgtaca aggattcga ttaggaaat ttttattgt tgtacaaat tggggttga acggcaatt tcctcaaca aattatgga atatgttga accggaaga ggtcgctag agggcgaac tccgggggt cttgcctcc tgtcgtgga aagagtgtc acacgaccc tctcggtat tttgcgaca cttttactt tctacacgg aaaccttaa ccgatcggg aaaattccg atactagtg agactttca cgcaaaatg atctctact caatgtcga agttactga aaaacgttg cggtggatt tagtcgaat tggcgtttc cgatgtgca ggtcaagca cgattccag cgactccgg aaattctgc atctgtaca gttcgtgtc tgaactgtg ttaaaatgt tgtacatgt tggatattc gcaaacagt tttgtccgc atgggttct ggacggcga aaaaaatcc acatttcgc aggatttgt cacgaagga attatttgt cgaagacga tcaggccgt tccatggct tggtgtgtg atatggacg atgaggaac acttgcccc cattgtcaa ccatcgagc aacgttcct tggcagttt tgcaggagt tgccgtttt tgatcgggt ttaatacaa gggttttgc ttttgaggg attggtgca ggaacagtt tgggcgaaa atgattacc agcgccttc ataatgaat ttctcaatg ataatctcg tggtgataa ggcggacgc atttgtacg aagatgcgt ttgaaaaat tgtcagtta agaatgaat ccgatctga ggcacaagg tgcgaattc agtttataa acagtgtgg gtttggagg aagctggga ttgacggtg gtgggattt ttaaggagt ttattaatg aagttctga aaactgcat ttgacccca accggggat ttttcctct taacggctg acaacacac tgtatccaa acccaaatg tccacttga ttgtggaaa atttcatgg aacactact atttcatcg gccgattag tcggaaaag caattttcg aaaacatac tagtggatc tacctttag ccgaatttt tcctagcca aattattag tagaccgag ccagtgctc actacttaa aatcactgg acccggtcc tctatcgaa atctcttgt atttgaggg attacccgg gcgatgtga gcgacctag gattggatt ttacgacag ttaataacg acttgggcg agactagag tcgttgagc ttaaacctc acgggagca acatccagg taacaaacg agaatcgac tcgagtaca ttcaacgat tggcggatt tgaaattaa acgttcaat tgaaaaaac aatgtatgg catttcggg agggtcttg acagtgtcg tgcccttgc tgtggttaa aactcttca accataacg agctgcaag tcatcatag ggggcgata cgcaggaaa ttgatttga gtgacttga aagctcaca ctgtatatg gaggcgaat ttacggccg atcacccca ctgtgaatc tgttctgga aaattttaa acacgttca ccgacactc agaaaaaaa tgttgctca agtttgtaa cgagttgtt caaggccgc cactgctgg gctttaaag aacttaatc cccagtttt gcattcaat cgtcaggga gtgaggatc ggatgccga ccgcaagca cgtgcttga atttactca aaataccaa tcattaagg aggaagaag ttttaagaa ataaactgc tgcaagcga ttgaacaac aggctggtt ttgagttat cgattacca ccatggact ttgacgata tgttagaag aattaggcg agttgggga aataccaaa tcacaacgt accttttag tctgtcttc ccgtatttt tcgcagcgg ccaatagtc tcagttacg tgtttacgg caggaatac caaattaca ggtgtttta tccccgagt gcgaagatg cagaaaatg caacttacg aagtccctt ggatcgact ttgccatac ctggaggcg actctaggg aaatattgg attcgaaat cccacctct gtgataggt ttaccgtaa atgaaacag tcgcgaatt acacttatt gttcgagag atttgttta caaatgtga ctgaaacgt gcaacgaat gggtgtttg atagcgagg agcggacaa tcgtaaatg attggaact tgacttgtg tggaaaatc aatggaaac tctcctttg tgggcacga gtcatttcg cggggatta tcgtgggat cgggattat ttgggattt tggccgaca ggtatggac gcaaaagaa tatttatca tctgtatcc tgatcatga gcgtgtcag gatgtgccc aagttgtct cacccgaat acataactt tcgtgatat taatgtttg tgaatgcga tgggcacgg ctggagtct accctttgg ctttcatta tcggggtcg aattggttg gcaaaagga agcgagaaa taaccggaa tcgtattaa actatttct acgctgttg gtgaggctg ttgttgccc ttgtcgcgt ggtacacca aagactggg ttttattac aactcatag tttctagtc cgtctattt tatttttca tatattact ggtttatac cagaatccg tgcggtggc tgctagcca acgaaaaac gcgaaaagg ccaaaggca tcgtttgca aagcggcga aagtaaaca acgtcactt tatccgagt ctttactgg cctcattca agaaagacg cagcctcga atagcgaca gtggccaag atgaaggaa caaaaattt tattcgtat tgaaaacga tcctaaaat cgcgtaaac tcgtggtca gaatgctga tcacgtatt tcatttggt ctgtgaacg catttgtat tctacggac tgtcggtga attcgacca gcatgagcg ggaacaaat acatcaatt ttgcgttag tgagcttgg ttgaaattc ccggttata caattgctt gggtcagta ttcaaaaaa tcgggcgcc ggttgtccc ttgttgggt ctttacttt tgtgtggtt tgacttgta ctttgacta ttttcgtac cttccgaaa tgacttggg cggttattc tgctatttc tcattggga aactgggga tcacagctg cgtttggtg tcgtttacg tccacacag ccgaaatgt tgccaacag tggtgcgga gcgggggag tgggatctg cgtcgacta tggcccgat ttggggcgc ttttagccc cctttgtcc ctcttctgg gaatttatg tcaaaccgt tgccgatgc tactgttcg ggggggttg cagtgctgg cgggaatct tggcgttga agttacctg aaactttgg gaaataaat tacctgaaa gtgtagaag aggctgaaa atttgtaaa taaagtgat ttttttacc acgagtgcg attattcag gatgag
