Status
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Gene information
General information
TC033023
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013932,TC013933
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2573359..2584673
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033023
    iBeetle screen iB_08208

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033023 RA
      MAFKMSYSF EGNYRTKPE QNLSGASKK ISRDVLLQK 
      THEERRKRQ EQRLKLQSA QLLQSHIRS YLVRKHKKE 
      EERAYYDNN FENIPIQVL LSKLLFFYD PVKDNMRLY 
      QISERLLQQ NVQINQALG SDTALSWLI KWWLVLCLK 
      SSYHPDMTK LMLNCLDAF VCNTPTIEY LTRKGYYVY 
      LRTLLDNDA KELLQQILS LIYRPFQSL RITQSSQDI 
      ILSEFCSNF LKPTFTNNV KTVLIPYLK NKRDFPYEK 
      IIRYLNSGY HFQISNSLF YCILALEPD DYEPTSDSI 
      QVLSTLSVD IHQLKPPVY LISDDSDDE SEVQVSEEE 
      LLLSDYLHI MNKPERVRK WLHFFEQNS NNEDVLMSL 
      TKFCHNLLL VYKDSIRKF LLLYKLGLN GNFLNKLWN 
      MLNRKRSLE GELRGSCLL SWKECHTTL SVFCDTFTF 
      YTETLTDRE NSDTSETFT QNDLYSMSK LLKNVAVDL 
      VELAFPMCR SSTIPATPE ILHLYSSCL NCVKMLYML 
      DIRKQFCPH GFWTAKKIH ISQDLSRRN YLSKTIRPF 
      HGLVCDMDD EEHLPPLST IEQRSLAVL QELPFLIGF 
      NTRVLLLRD WCRNSLGEN DYQRLHNEF LNDNLVVIR 
      RTHLYEDAF EKLSVKNES DLRHKVRIQ FINSVGLEE 
      AGIDGGGIF KEFINEVLK TAFDPNRGF FLLTADNTL 
      YPNPNVHLI VENFMEHYY FIGRLVGKA IFENILVDL 
      PLAEFFLAK LLVDRASAH YLKSLDPVL YRNLLYLRD 
      YPGDVSDLG LDFTTVNND LGETRVVEL KPHGSNIQV 
      TNENRLEYI QRLADLKLN VQLKKQCMA FREGLDSVV 
      PLLWLKLFN HNELQVIIG GDTQEIDLS DLKAHTVYG 
      GEFTADHPT VNLFWKILN TFTDTQKKM LLKFVTSCS 
      RPPLLGFKE LNPQFCIQS SGSEDRMPT ASTCLNLLK 
      IPIIKEEEV LRNKLLQAI EQQAGFELS ITTMDFDDM 
      LEELGELGK YQITTYLLV CLPVFFAAA NSLSYVFTA 
      GIPNYRCFI PECEDAENA TYEVPWIDF AIPGGDSRE 
      ILDSKSHLC DRFTVNETV ANYTYCSRD LFTNVTETC 
      NEWVFDSEE RTIVNDWNL TCVENQWKL SFVGTSHFA 
      GIIVGSGLF GILADRYGR KRIFIICIL IMSVSGCAQ 
      VVSPEYITF VILMFVNAM GTAGVYPLA FIIGVELVG 
      KRKREITGI VLNYFYAVG EAVVALVAW YTKDWVLLQ 
      LIVSSPSIL FFIYYWFIP ESVRWLLAN EKREKAKGI 
      VCKAAKVNN VTLSESLLA SFKKDAASN SDSGQDEGT 
      KILFVLKTI LKSRKLVVR MLITYFIWS VNAFVFYGL 
      SVNSTSMSG NKYINFALV SLVEIPGYT IAWVSIQKI 
      GRRLSLVGS LLLCGLTCT LTIFVPSEM TWAVILLFL 
      IGKLGITAA FGVVYVHTA EMLPTVVRS GGVGSASTM 
      ARFGALLAP FVPLLGIYV KPLPMLLFG GVAVLAGIL 
      ALKLPETLG NKLPESVEE AENL
    CDS sequence
    • >TC033023 RA
      atggcgttt aaaatgtca tatagtttt gaaggcaac 
      tacaggaca aagcctgag caaaatttg tcaggcgct 
      agtaaaaaa atttcgagg gatgttttg ctacaaaaa 
      acccacgaa gagagacgt aaacgacag gagcaacgt 
      ctgaagtta caaagtgct caacttttg cagtcacac 
      attaggagt tatttagtg cgcaaacat aaaaaggag 
      gaggaaagg gcgtactat gataacaat tttgaaaat 
      atcccgata caggtgttg ttgtccaaa cttctgttc 
      ttttatgac ccggttaag gacaacatg cggctgtac 
      caaatatcc gaacgattg ttgcaacaa aatgtgcaa 
      atcaatcaa gcgcttggc tcagatacg gccctttcg 
      tggctcatc aaatggtgg ctggttttg tgtctcaaa 
      agctcgtat caccctgat atgacaaag ctcatgtta 
      aattgcctt gacgcgttc gtttgcaac acaccgaca 
      attgaatat ctcacacgc aaaggttac tacgtgtac 
      ctgcgtact ctcctggat aatgacgct aaagagtta 
      cttcaacaa atcctaagt ctgatatat cgccccttc 
      caatcgcta cgaatcacc cagagttcg caggacatc 
      attttgtcc gaattctgc agcaatttc ctcaaaccc 
      accttcaca aacaacgta aagactgtc cttataccg 
      tatttaaaa aacaaacgc gattttcca tacgaaaaa 
      atcataagg tacttgaat agtggctac cacttccaa 
      atcagcaat agtttgttt tactgcatt ttggcgctt 
      gaacccgac gattacgaa cccacaagt gatagtatt 
      caagtcttg tccacactt agcgtggac atccaccag 
      ttgaaaccc ccggtgtat ttaataagc gacgatagt 
      gacgatgag agcgaagtc caggtctcg gaagaagaa 
      ttactcctc tctgactat ttacatatc atgaataag 
      ccggaacgg gtccggaaa tggttgcac tttttcgaa 
      caaaatagc aataatgaa gatgtgcta atgtcgctg 
      acgaagttt tgccacaat ctcctactt gtgtacaag 
      gattcgatt aggaaattt ttattgttg tacaaattg 
      gggttgaac ggcaatttc ctcaacaaa ttatggaat 
      atgttgaac cggaagagg tcgctagag ggcgaactc 
      cgggggtct tgcctcctg tcgtggaaa gagtgtcac 
      acgaccctc tcggtattt tgcgacact tttactttc 
      tacacggaa accttaacc gatcgggaa aattccgat 
      actagtgag actttcacg caaaatgat ctctactca 
      atgtcgaag ttactgaaa aacgttgcg gtggattta 
      gtcgaattg gcgtttccg atgtgcagg tcaagcacg 
      attccagcg actccggaa attctgcat ctgtacagt 
      tcgtgtctg aactgtgtt aaaatgttg tacatgttg 
      gatattcgc aaacagttt tgtccgcat gggttctgg 
      acggcgaaa aaaatccac atttcgcag gatttgtca 
      cgaaggaat tatttgtcg aagacgatc aggccgttc 
      catggcttg gtgtgtgat atggacgat gaggaacac 
      ttgccccca ttgtcaacc atcgagcaa cgttccttg 
      gcagttttg caggagttg ccgtttttg atcgggttt 
      aatacaagg gttttgctt ttgagggat tggtgcagg 
      aacagtttg ggcgaaaat gattaccag cgccttcat 
      aatgaattt ctcaatgat aatctcgtg gtgataagg 
      cggacgcat ttgtacgaa gatgcgttt gaaaaattg 
      tcagttaag aatgaatcc gatctgagg cacaaggtg 
      cgaattcag tttataaac agtgtgggt ttggaggaa 
      gctgggatt gacggtggt gggattttt aaggagttt 
      attaatgaa gttctgaaa actgcattt gaccccaac 
      cggggattt ttcctctta acggctgac aacacactg 
      tatccaaac ccaaatgtc cacttgatt gtggaaaat 
      ttcatggaa cactactat ttcatcggc cgattagtc 
      ggaaaagca attttcgaa aacatacta gtggatcta 
      cctttagcc gaatttttc ctagccaaa ttattagta 
      gaccgagcc agtgctcac tacttaaaa tcactggac 
      ccggtcctc tatcgaaat ctcttgtat ttgagggat 
      tacccgggc gatgtgagc gacctagga ttggatttt 
      acgacagtt aataacgac ttgggcgag actagagtc 
      gttgagctt aaacctcac gggagcaac atccaggta 
      acaaacgag aatcgactc gagtacatt caacgattg 
      gcggatttg aaattaaac gttcaattg aaaaaacaa 
      tgtatggca tttcgggag ggtcttgac agtgtcgtg 
      cccttgctg tggttaaaa ctcttcaac cataacgag 
      ctgcaagtc atcataggg ggcgatacg caggaaatt 
      gatttgagt gacttgaaa gctcacact gtatatgga 
      ggcgaattt acggccgat caccccact gtgaatctg 
      ttctggaaa attttaaac acgttcacc gacactcag 
      aaaaaaatg ttgctcaag tttgtaacg agttgttca 
      aggccgcca ctgctgggc tttaaagaa cttaatccc 
      cagttttgc attcaatcg tcagggagt gaggatcgg 
      atgccgacc gcaagcacg tgcttgaat ttactcaaa 
      ataccaatc attaaggag gaagaagtt ttaagaaat 
      aaactgctg caagcgatt gaacaacag gctggtttt 
      gagttatcg attaccacc atggacttt gacgatatg 
      ttagaagaa ttaggcgag ttggggaaa taccaaatc 
      acaacgtac cttttagtc tgtcttccc gtatttttc 
      gcagcggcc aatagtctc agttacgtg tttacggca 
      ggaatacca aattacagg tgttttatc cccgagtgc 
      gaagatgca gaaaatgca acttacgaa gtcccttgg 
      atcgacttt gccatacct ggaggcgac tctagggaa 
      atattggat tcgaaatcc cacctctgt gataggttt 
      accgtaaat gaaacagtc gcgaattac acttattgt 
      tcgagagat ttgtttaca aatgtgact gaaacgtgc 
      aacgaatgg gtgtttgat agcgaggag cggacaatc 
      gtaaatgat tggaacttg acttgtgtg gaaaatcaa 
      tggaaactc tcctttgtg ggcacgagt catttcgcg 
      gggattatc gtgggatcg ggattattt gggattttg 
      gccgacagg tatggacgc aaaagaata tttatcatc 
      tgtatcctg atcatgagc gtgtcagga tgtgcccaa 
      gttgtctca cccgaatac ataactttc gtgatatta 
      atgtttgtg aatgcgatg ggcacggct ggagtctac 
      cctttggct ttcattatc ggggtcgaa ttggttggc 
      aaaaggaag cgagaaata accggaatc gtattaaac 
      tatttctac gctgttggt gaggctgtt gttgccctt 
      gtcgcgtgg tacaccaaa gactgggtt ttattacaa 
      ctcatagtt tctagtccg tctatttta tttttcata 
      tattactgg tttatacca gaatccgtg cggtggctg 
      ctagccaac gaaaaacgc gaaaaggcc aaaggcatc 
      gtttgcaaa gcggcgaaa gtaaacaac gtcacttta 
      tccgagtct ttactggcc tcattcaag aaagacgca 
      gcctcgaat agcgacagt ggccaagat gaaggaaca 
      aaaatttta ttcgtattg aaaacgatc ctaaaatcg 
      cgtaaactc gtggtcaga atgctgatc acgtatttc 
      atttggtct gtgaacgca tttgtattc tacggactg 
      tcggtgaat tcgaccagc atgagcggg aacaaatac 
      atcaatttt gcgttagtg agcttggtt gaaattccc 
      ggttataca attgcttgg gtcagtatt caaaaaatc 
      gggcgccgg ttgtccctt gttgggtct ttacttttg 
      tgtggtttg acttgtact ttgactatt ttcgtacct 
      tccgaaatg acttgggcg gttattctg ctatttctc 
      attgggaaa ctggggatc acagctgcg tttggtgtc 
      gtttacgtc cacacagcc gaaatgttg ccaacagtg 
      gtgcggagc gggggagtg ggatctgcg tcgactatg 
      gcccgattt ggggcgctt ttagccccc tttgtccct 
      cttctggga atttatgtc aaaccgttg ccgatgcta 
      ctgttcggg ggggttgca gtgctggcg ggaatcttg 
      gcgttgaag ttacctgaa actttggga aataaatta 
      cctgaaagt gtagaagag gctgaaaat ttgtaa
    mRNA sequence
    • >TC033023 RA
      aaattccat tattttgta atttgtttt tgtacaaaa 
      agcgaccaa aattttgca aaatggcgt ttaaaatgt 
      catatagtt ttgaaggca actacagga caaagcctg 
      agcaaaatt tgtcaggcg ctagtaaaa aaatttcga 
      gggatgttt tgctacaaa aaacccacg aagagagac 
      gtaaacgac aggagcaac gtctgaagt tacaaagtg 
      ctcaacttt tgcagtcac acattagga gttatttag 
      tgcgcaaac ataaaaagg aggaggaaa gggcgtact 
      atgataaca attttgaaa atatcccga tacaggtgt 
      tgttgtcca aacttctgt tcttttatg acccggtta 
      aggacaaca tgcggctgt accaaatat ccgaacgat 
      tgttgcaac aaaatgtgc aaatcaatc aagcgcttg 
      gctcagata cggcccttt cgtggctca tcaaatggt 
      ggctggttt tgtgtctca aaagctcgt atcaccctg 
      atatgacaa agctcatgt taaattgcc ttgacgcgt 
      tcgtttgca acacaccga caattgaat atctcacac 
      gcaaaggtt actacgtgt acctgcgta ctctcctgg 
      ataatgacg ctaaagagt tacttcaac aaatcctaa 
      gtctgatat atcgcccct tccaatcgc tacgaatca 
      cccagagtt cgcaggaca tcattttgt ccgaattct 
      gcagcaatt tcctcaaac ccaccttca caaacaacg 
      taaagactg tccttatac cgtatttaa aaaacaaac 
      gcgattttc catacgaaa aaatcataa ggtacttga 
      atagtggct accacttcc aaatcagca atagtttgt 
      tttactgca ttttggcgc ttgaacccg acgattacg 
      aacccacaa gtgatagta ttcaagtct tgtccacac 
      ttagcgtgg acatccacc agttgaaac ccccggtgt 
      atttaataa gcgacgata gtgacgatg agagcgaag 
      tccaggtct cggaagaag aattactcc tctctgact 
      atttacata tcatgaata agccggaac gggtccgga 
      aatggttgc actttttcg aacaaaata gcaataatg 
      aagatgtgc taatgtcgc tgacgaagt tttgccaca 
      atctcctac ttgtgtaca aggattcga ttaggaaat 
      ttttattgt tgtacaaat tggggttga acggcaatt 
      tcctcaaca aattatgga atatgttga accggaaga 
      ggtcgctag agggcgaac tccgggggt cttgcctcc 
      tgtcgtgga aagagtgtc acacgaccc tctcggtat 
      tttgcgaca cttttactt tctacacgg aaaccttaa 
      ccgatcggg aaaattccg atactagtg agactttca 
      cgcaaaatg atctctact caatgtcga agttactga 
      aaaacgttg cggtggatt tagtcgaat tggcgtttc 
      cgatgtgca ggtcaagca cgattccag cgactccgg 
      aaattctgc atctgtaca gttcgtgtc tgaactgtg 
      ttaaaatgt tgtacatgt tggatattc gcaaacagt 
      tttgtccgc atgggttct ggacggcga aaaaaatcc 
      acatttcgc aggatttgt cacgaagga attatttgt 
      cgaagacga tcaggccgt tccatggct tggtgtgtg 
      atatggacg atgaggaac acttgcccc cattgtcaa 
      ccatcgagc aacgttcct tggcagttt tgcaggagt 
      tgccgtttt tgatcgggt ttaatacaa gggttttgc 
      ttttgaggg attggtgca ggaacagtt tgggcgaaa 
      atgattacc agcgccttc ataatgaat ttctcaatg 
      ataatctcg tggtgataa ggcggacgc atttgtacg 
      aagatgcgt ttgaaaaat tgtcagtta agaatgaat 
      ccgatctga ggcacaagg tgcgaattc agtttataa 
      acagtgtgg gtttggagg aagctggga ttgacggtg 
      gtgggattt ttaaggagt ttattaatg aagttctga 
      aaactgcat ttgacccca accggggat ttttcctct 
      taacggctg acaacacac tgtatccaa acccaaatg 
      tccacttga ttgtggaaa atttcatgg aacactact 
      atttcatcg gccgattag tcggaaaag caattttcg 
      aaaacatac tagtggatc tacctttag ccgaatttt 
      tcctagcca aattattag tagaccgag ccagtgctc 
      actacttaa aatcactgg acccggtcc tctatcgaa 
      atctcttgt atttgaggg attacccgg gcgatgtga 
      gcgacctag gattggatt ttacgacag ttaataacg 
      acttgggcg agactagag tcgttgagc ttaaacctc 
      acgggagca acatccagg taacaaacg agaatcgac 
      tcgagtaca ttcaacgat tggcggatt tgaaattaa 
      acgttcaat tgaaaaaac aatgtatgg catttcggg 
      agggtcttg acagtgtcg tgcccttgc tgtggttaa 
      aactcttca accataacg agctgcaag tcatcatag 
      ggggcgata cgcaggaaa ttgatttga gtgacttga 
      aagctcaca ctgtatatg gaggcgaat ttacggccg 
      atcacccca ctgtgaatc tgttctgga aaattttaa 
      acacgttca ccgacactc agaaaaaaa tgttgctca 
      agtttgtaa cgagttgtt caaggccgc cactgctgg 
      gctttaaag aacttaatc cccagtttt gcattcaat 
      cgtcaggga gtgaggatc ggatgccga ccgcaagca 
      cgtgcttga atttactca aaataccaa tcattaagg 
      aggaagaag ttttaagaa ataaactgc tgcaagcga 
      ttgaacaac aggctggtt ttgagttat cgattacca 
      ccatggact ttgacgata tgttagaag aattaggcg 
      agttgggga aataccaaa tcacaacgt accttttag 
      tctgtcttc ccgtatttt tcgcagcgg ccaatagtc 
      tcagttacg tgtttacgg caggaatac caaattaca 
      ggtgtttta tccccgagt gcgaagatg cagaaaatg 
      caacttacg aagtccctt ggatcgact ttgccatac 
      ctggaggcg actctaggg aaatattgg attcgaaat 
      cccacctct gtgataggt ttaccgtaa atgaaacag 
      tcgcgaatt acacttatt gttcgagag atttgttta 
      caaatgtga ctgaaacgt gcaacgaat gggtgtttg 
      atagcgagg agcggacaa tcgtaaatg attggaact 
      tgacttgtg tggaaaatc aatggaaac tctcctttg 
      tgggcacga gtcatttcg cggggatta tcgtgggat 
      cgggattat ttgggattt tggccgaca ggtatggac 
      gcaaaagaa tatttatca tctgtatcc tgatcatga 
      gcgtgtcag gatgtgccc aagttgtct cacccgaat 
      acataactt tcgtgatat taatgtttg tgaatgcga 
      tgggcacgg ctggagtct accctttgg ctttcatta 
      tcggggtcg aattggttg gcaaaagga agcgagaaa 
      taaccggaa tcgtattaa actatttct acgctgttg 
      gtgaggctg ttgttgccc ttgtcgcgt ggtacacca 
      aagactggg ttttattac aactcatag tttctagtc 
      cgtctattt tatttttca tatattact ggtttatac 
      cagaatccg tgcggtggc tgctagcca acgaaaaac 
      gcgaaaagg ccaaaggca tcgtttgca aagcggcga 
      aagtaaaca acgtcactt tatccgagt ctttactgg 
      cctcattca agaaagacg cagcctcga atagcgaca 
      gtggccaag atgaaggaa caaaaattt tattcgtat 
      tgaaaacga tcctaaaat cgcgtaaac tcgtggtca 
      gaatgctga tcacgtatt tcatttggt ctgtgaacg 
      catttgtat tctacggac tgtcggtga attcgacca 
      gcatgagcg ggaacaaat acatcaatt ttgcgttag 
      tgagcttgg ttgaaattc ccggttata caattgctt 
      gggtcagta ttcaaaaaa tcgggcgcc ggttgtccc 
      ttgttgggt ctttacttt tgtgtggtt tgacttgta 
      ctttgacta ttttcgtac cttccgaaa tgacttggg 
      cggttattc tgctatttc tcattggga aactgggga 
      tcacagctg cgtttggtg tcgtttacg tccacacag 
      ccgaaatgt tgccaacag tggtgcgga gcgggggag 
      tgggatctg cgtcgacta tggcccgat ttggggcgc 
      ttttagccc cctttgtcc ctcttctgg gaatttatg 
      tcaaaccgt tgccgatgc tactgttcg ggggggttg 
      cagtgctgg cgggaatct tggcgttga agttacctg 
      aaactttgg gaaataaat tacctgaaa gtgtagaag 
      aggctgaaa atttgtaaa taaagtgat ttttttacc 
      acgagtgcg attattcag gatgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY