Status
Please wait ...
Gene information
General information
TC001613 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1327031..1338309
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001613
    iBeetle screen iB_08207

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001613 RA
      MEYQSKDVI LPRNDRKGR KPWRALVSY WILGLCNNY 
      GYVVMLTAA SDIIGESEG AKSTERNCT YMSTGAILL 
      ADIIPSLLV KLLAPFLPF FVNVRVAVC ILCSSAGFL 
      LVAFSQNIT MSLLGVVAT SFCSGLGEV TYLQYSSFY 
      DKNVVSTWS SGTGGAGVI GAVSYSLLQ QLGLKTTLL 
      VMLLVPGMM AVTFYIILP SPEVMDEND IQADVNNEE 
      IKNPKEAFM KKLKHVPSL FKYMIPFGL VYFLEYYIN 
      QGTFELINF ENTFLAPSD QYRWLQVAY QIGVFVSRS 
      SVNFFHIRH IWVMSVLQA VNVVIFTTE VIYYYMPSF 
      WIILALTLW EGLLGGAAY VNTFYRIST EVREENKQF 
      SMAITSFAD SIGITLAGI VSIYAHNAI CSLPKPQR
    • >TC001613 RB
      MDLIPFEFA GAFLGLGSK RPKKGDYAA MEYQSKDVI 
      LPRNDRKGR KPWRALVSY WILGLCNNY GYVVMLTAA 
      SDIIGESEG AKSTERNCT YMSTGAILL ADIIPSLLV 
      KLLAPFLPF FVNVRVAVC ILCSSAGFL LVAFSQNIT 
      MSLLGVVAT SFCSGLGEV TYLQYSSFY DKNVVSTWS 
      SGTGGAGVI GAVSYSLLQ QLGLKTTLL VMLLVPGMM 
      AVTFYIILP SPEVMDEND IQADVNNEE IKNPKEAFM 
      KKLKHVPSL FKYMIPFGL VYFLEYYIN QGTFELINF 
      ENTFLAPSD QYRWLQVAY QIGVFVSRS SVNFFHIRH 
      IWVMSVLQA VNVVIFTTE VIYYYMPSF WIILALTLW 
      EGLLGGAAY VNTFYRIST EVREENKQF SMAITSFAD 
      SIGITLAGI VSIYAHNAI CSLPKPQR
    CDS sequence
    • >TC001613 RA
      atggagtac cagagcaag gacgtgatt ttgccacga 
      aacgaccgc aaagggcgc aagccgtgg cgggccctc 
      gtctcctac tggatcctc gggctctgc aacaactac 
      ggatacgtc gtgatgctc acggcggcc agtgacatc 
      atcggagag tccgaaggc gcaaaaagc actgaaaga 
      aactgtaca tatatgtct acaggggcc attttacta 
      gccgacatt atcccaagc ttgctagtt aagttactg 
      gcgccgttc ctccctttt ttcgtaaat gtaagggtt 
      gccgtttgt atactttgt tcctcagcg ggtttcctt 
      cttgtagcc tttagccaa aacattaca atgtccttg 
      ttaggagta gtcgctacg tccttctgc tcagggctt 
      ggggaagtg acgtatctc caatacagc tcgttctat 
      gacaagaat gtcgtttcg acgtggagt tcgggcacc 
      ggcggcgct ggtgttatc ggtgccgtc tcatactcg 
      ctcctgcaa cagttaggg ctcaaaacc accctatta 
      gtgatgctg ttggtccca gggatgatg gctgtgacg 
      ttttatata attttgccg agtccggaa gtcatggac 
      gaaaatgac atacaagcc gacgtgaac aatgaagaa 
      atcaaaaat ccaaaagaa gccttcatg aaaaaactc 
      aaacacgtt ccctcgctt ttcaagtac atgattccg 
      ttcggtttg gtttacttc ttggagtat tacataaac 
      cagggaact ttcgaattg attaatttc gaaaatacg 
      tttttggca cccagtgac cagtacagg tggcttcag 
      gtcgcttat cagatcggg gtgtttgtg tcccggtct 
      tcggtaaat ttcttccat ataagacat atatgggtc 
      atgtcagta ttacaggct gttaacgtg gtgattttc 
      accacggaa gtcatatat tattatatg ccgagtttt 
      tggatcata ttggcccta acgttgtgg gagggtctc 
      ttgggaggt gctgcatac gtgaacact ttctatagg 
      atctcgact gaggtccgt gaagagaat aagcagttt 
      tcgatggca attactagt tttgccgac agtattggc 
      ataactcta gccggaatc gtatcgatt tacgcacac 
      aacgccatc tgtagcctg ccgaagcct caacgttag
    • >TC001613 RB
      atggacttg atccctttc gagttcgct ggagccttc 
      ctcggacta ggctcgaaa cggcccaaa aaaggcgac 
      tacgctgcg atggagtac cagagcaag gacgtgatt 
      ttgccacga aacgaccgc aaagggcgc aagccgtgg 
      cgggccctc gtctcctac tggatcctc gggctctgc 
      aacaactac ggatacgtc gtgatgctc acggcggcc 
      agtgacatc atcggagag tccgaaggc gcaaaaagc 
      actgaaaga aactgtaca tatatgtct acaggggcc 
      attttacta gccgacatt atcccaagc ttgctagtt 
      aagttactg gcgccgttc ctccctttt ttcgtaaat 
      gtaagggtt gccgtttgt atactttgt tcctcagcg 
      ggtttcctt cttgtagcc tttagccaa aacattaca 
      atgtccttg ttaggagta gtcgctacg tccttctgc 
      tcagggctt ggggaagtg acgtatctc caatacagc 
      tcgttctat gacaagaat gtcgtttcg acgtggagt 
      tcgggcacc ggcggcgct ggtgttatc ggtgccgtc 
      tcatactcg ctcctgcaa cagttaggg ctcaaaacc 
      accctatta gtgatgctg ttggtccca gggatgatg 
      gctgtgacg ttttatata attttgccg agtccggaa 
      gtcatggac gaaaatgac atacaagcc gacgtgaac 
      aatgaagaa atcaaaaat ccaaaagaa gccttcatg 
      aaaaaactc aaacacgtt ccctcgctt ttcaagtac 
      atgattccg ttcggtttg gtttacttc ttggagtat 
      tacataaac cagggaact ttcgaattg attaatttc 
      gaaaatacg tttttggca cccagtgac cagtacagg 
      tggcttcag gtcgcttat cagatcggg gtgtttgtg 
      tcccggtct tcggtaaat ttcttccat ataagacat 
      atatgggtc atgtcagta ttacaggct gttaacgtg 
      gtgattttc accacggaa gtcatatat tattatatg 
      ccgagtttt tggatcata ttggcccta acgttgtgg 
      gagggtctc ttgggaggt gctgcatac gtgaacact 
      ttctatagg atctcgact gaggtccgt gaagagaat 
      aagcagttt tcgatggca attactagt tttgccgac 
      agtattggc ataactcta gccggaatc gtatcgatt 
      tacgcacac aacgccatc tgtagcctg ccgaagcct 
      caacgttag
    mRNA sequence
    • >TC001613 RA
      gcacgttcg aaaataata aatatttgt gccggcatt 
      taatgagca gttcgtccg gagtctcat tgattttga 
      gttcatcgg gaactattt ttacctccc atgtgccaa 
      ttcgacccg tgcctaagg atttatctc tagatttgc 
      cattgtgta aggactagg ctcgaaacg gcccaaaaa 
      aggcgacta cgctgcgat ggagtacca gagcaagga 
      cgtgatttt gccacgaaa cgaccgcaa agggcgcaa 
      gccgtggcg ggccctcgt ctcctactg gatcctcgg 
      gctctgcaa caactacgg atacgtcgt gatgctcac 
      ggcggccag tgacatcat cggagagtc cgaaggcgc 
      aaaaagcac tgaaagaaa ctgtacata tatgtctac 
      aggggccat tttactagc cgacattat cccaagctt 
      gctagttaa gttactggc gccgttcct ccctttttt 
      cgtaaatgt aagggttgc cgtttgtat actttgttc 
      ctcagcggg tttccttct tgtagcctt tagccaaaa 
      cattacaat gtccttgtt aggagtagt cgctacgtc 
      cttctgctc agggcttgg ggaagtgac gtatctcca 
      atacagctc gttctatga caagaatgt cgtttcgac 
      gtggagttc gggcaccgg cggcgctgg tgttatcgg 
      tgccgtctc atactcgct cctgcaaca gttagggct 
      caaaaccac cctattagt gatgctgtt ggtcccagg 
      gatgatggc tgtgacgtt ttatataat tttgccgag 
      tccggaagt catggacga aaatgacat acaagccga 
      cgtgaacaa tgaagaaat caaaaatcc aaaagaagc 
      cttcatgaa aaaactcaa acacgttcc ctcgctttt 
      caagtacat gattccgtt cggtttggt ttacttctt 
      ggagtatta cataaacca gggaacttt cgaattgat 
      taatttcga aaatacgtt tttggcacc cagtgacca 
      gtacaggtg gcttcaggt cgcttatca gatcggggt 
      gtttgtgtc ccggtcttc ggtaaattt cttccatat 
      aagacatat atgggtcat gtcagtatt acaggctgt 
      taacgtggt gattttcac cacggaagt catatatta 
      ttatatgcc gagtttttg gatcatatt ggccctaac 
      gttgtggga gggtctctt gggaggtgc tgcatacgt 
      gaacacttt ctataggat ctcgactga ggtccgtga 
      agagaataa gcagttttc gatggcaat tactagttt 
      tgccgacag tattggcat aactctagc cggaatcgt 
      atcgattta cgcacacaa cgccatctg tagcctgcc 
      gaagcctca acgttagtt ttgtaaagg ttttattta 
      ataaataaa tgatgcgtt t
    • >TC001613 RB
      gcttttatt taacattac gcaacttta catttttac 
      ggtggatat ggacttgat ccctttcga gttcgctgg 
      agccttcct cggactagg ctcgaaacg gcccaaaaa 
      aggcgacta cgctgcgat ggagtacca gagcaagga 
      cgtgatttt gccacgaaa cgaccgcaa agggcgcaa 
      gccgtggcg ggccctcgt ctcctactg gatcctcgg 
      gctctgcaa caactacgg atacgtcgt gatgctcac 
      ggcggccag tgacatcat cggagagtc cgaaggcgc 
      aaaaagcac tgaaagaaa ctgtacata tatgtctac 
      aggggccat tttactagc cgacattat cccaagctt 
      gctagttaa gttactggc gccgttcct ccctttttt 
      cgtaaatgt aagggttgc cgtttgtat actttgttc 
      ctcagcggg tttccttct tgtagcctt tagccaaaa 
      cattacaat gtccttgtt aggagtagt cgctacgtc 
      cttctgctc agggcttgg ggaagtgac gtatctcca 
      atacagctc gttctatga caagaatgt cgtttcgac 
      gtggagttc gggcaccgg cggcgctgg tgttatcgg 
      tgccgtctc atactcgct cctgcaaca gttagggct 
      caaaaccac cctattagt gatgctgtt ggtcccagg 
      gatgatggc tgtgacgtt ttatataat tttgccgag 
      tccggaagt catggacga aaatgacat acaagccga 
      cgtgaacaa tgaagaaat caaaaatcc aaaagaagc 
      cttcatgaa aaaactcaa acacgttcc ctcgctttt 
      caagtacat gattccgtt cggtttggt ttacttctt 
      ggagtatta cataaacca gggaacttt cgaattgat 
      taatttcga aaatacgtt tttggcacc cagtgacca 
      gtacaggtg gcttcaggt cgcttatca gatcggggt 
      gtttgtgtc ccggtcttc ggtaaattt cttccatat 
      aagacatat atgggtcat gtcagtatt acaggctgt 
      taacgtggt gattttcac cacggaagt catatatta 
      ttatatgcc gagtttttg gatcatatt ggccctaac 
      gttgtggga gggtctctt gggaggtgc tgcatacgt 
      gaacacttt ctataggat ctcgactga ggtccgtga 
      agagaataa gcagttttc gatggcaat tactagttt 
      tgccgacag tattggcat aactctagc cggaatcgt 
      atcgattta cgcacacaa cgccatctg tagcctgcc 
      gaagcctca acgttagtt ttgtaaagg ttttattta 
      ataaataaa tgatgcgtt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY