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Gene information
General information
TC010936 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1500054..1504630
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010936
    iBeetle screen iB_08196

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010936 RA
      MPSPIATIL FCSLLWLSV SETHTLFLG RNNTDVRII 
      GGNVAFAGQ FPYMAAIYK HTSSGNFFC GGALMSNQW 
      ILTAGSCVE GAQLFTIYL GTANLKDVN NPNSLKLAT 
      DEYVLHPDY NPDTFENDV GLIKLRMPI TFSVYIRPF 
      NYLPTFDLL PGTPGVVAM GWGQTSDDD PGLVDDLRF 
      VYLTPLSND ECKLVYGNQ IKDEMVCAA GNNNEGFCL 
      GDSGTPLIR IRQGPTATH VGIASFQSK NGCESTDPS 
      GYTRTLEYA DWIRNVTGV DNNKTKNAK PRIIGGEVA 
      FAGQFPYIA AIYKSTNTG TFFCGGALM NNQWILTSG 
      YCVDGAVLF TIYLGAHNL KANDPNVQK FATDTFVLH 
      PNYNLDTLE NDIGVIKLR LPVTFTDYI KPIDFLPSY 
      DLLPNMGGI MNLGWGQLN DETAGITDE LHYVYLIPI 
      SNEECQLSF GSQILDTMV CAGGNFNEG FWKVCHNNT 
      GFV
    CDS sequence
    • >TC010936 RA
      atgccgtcc ccaattgcc acgatttta ttttgttca 
      ttattatgg ctaagtgtt tctgaaaca cacacactc 
      tttctgggt agaaacaac acagatgtt cgtatcatt 
      ggaggcaat gttgcattt gcgggccaa ttcccttac 
      atggcagca atttataag cataccagc agtggcaac 
      tttttttgc ggaggtgcc cttatgagt aatcaatgg 
      atattgaca gcaggatca tgcgtcgaa ggtgcccaa 
      ctgtttacg atttatttg ggaacagca aacttaaaa 
      gatgtcaat aaccccaac agccttaaa ctagccaca 
      gatgaatac gttttgcat ccagattac aacccagac 
      acttttgaa aacgacgta ggtctcata aaattaaga 
      atgccgata acttttagc gtgtacata cggccgttc 
      aattattta ccaaccttt gaccttttg cctggcaca 
      cctggcgtg gtggccatg ggttggggc caaacgagt 
      gatgatgat cctggtctt gtcgacgac ctacgattt 
      gtatatttg acaccttta tcaaatgat gagtgtaaa 
      ttagtttac gggaatcaa atcaaagac gagatggtt 
      tgcgctgct ggcaataat aacgaagga ttttgcttg 
      ggcgatagt gggacaccc ttgattcga atcagacaa 
      ggcccgact gcaacccat gtcggaatt gcaagtttt 
      cagagcaag aacggatgc gagagcacc gatccttcg 
      ggatacacg aggactttg gaatacgct gactggata 
      aggaacgtg acgggagtt gacaacaac aaaaccaaa 
      aatgcaaaa ccacggatt attgggggt gaagtagca 
      tttgccggc caatttcca tacattgct gccatttac 
      aaaagtacc aacactggt acgttcttt tgtggaggt 
      gcccttatg aacaaccag tggatttta acgtcgggc 
      tattgtgta gatggagct gttttattt acgatttat 
      ttaggagca cacaatttg aaagcaaac gatccaaac 
      gttcaaaaa tttgccaca gacacgttc gttttgcat 
      ccaaattac aaccttgac acattagaa aatgatatt 
      ggggtaata aagctgcga ttgccagtg acatttaca 
      gattatata aaaccgatc gattttttg ccaagttat 
      gaccttttg cccaacatg ggcggaatt atgaacttg 
      ggatggggc caacttaac gacgaaacg gctggaatc 
      accgatgag ctccattat gtctattta attccaatt 
      tctaatgaa gagtgccaa ctcagcttt ggtagtcaa 
      attttggac acgatggtg tgtgctggt ggtaatttt 
      aacgaaggt ttttggaag gtttgccac aacaacact 
      ggttttgtt tga
    mRNA sequence
    • >TC010936 RA
      agtggtaaa ttggtttac cttcttttt gacaatgcc 
      gtccccaat tgccacgat tttattttg ttcattatt 
      atggctaag tgtttctga aacacacac actctttct 
      gggtagaaa caacacaga tgttcgtat cattggagg 
      caatgttgc atttgcggg ccaattccc ttacatggc 
      agcaattta taagcatac cagcagtgg caacttttt 
      ttgcggagg tgcccttat gagtaatca atggatatt 
      gacagcagg atcatgcgt cgaaggtgc ccaactgtt 
      tacgattta tttgggaac agcaaactt aaaagatgt 
      caataaccc caacagcct taaactagc cacagatga 
      atacgtttt gcatccaga ttacaaccc agacacttt 
      tgaaaacga cgtaggtct cataaaatt aagaatgcc 
      gataacttt tagcgtgta catacggcc gttcaatta 
      tttaccaac ctttgacct tttgcctgg cacacctgg 
      cgtggtggc catgggttg gggccaaac gagtgatga 
      tgatcctgg tcttgtcga cgacctacg atttgtata 
      tttgacacc tttatcaaa tgatgagtg taaattagt 
      ttacgggaa tcaaatcaa agacgagat ggtttgcgc 
      tgctggcaa taataacga aggattttg cttgggcga 
      tagtgggac acccttgat tcgaatcag acaaggccc 
      gactgcaac ccatgtcgg aattgcaag ttttcagag 
      caagaacgg atgcgagag caccgatcc ttcgggata 
      cacgaggac tttggaata cgctgactg gataaggaa 
      cgtgacggg agttgacaa caacaaaac caaaaatgc 
      aaaaccacg gattattgg gggtgaagt agcatttgc 
      cggccaatt tccatacat tgctgccat ttacaaaag 
      taccaacac tggtacgtt cttttgtgg aggtgccct 
      tatgaacaa ccagtggat tttaacgtc gggctattg 
      tgtagatgg agctgtttt atttacgat ttatttagg 
      agcacacaa tttgaaagc aaacgatcc aaacgttca 
      aaaatttgc cacagacac gttcgtttt gcatccaaa 
      ttacaacct tgacacatt agaaaatga tattggggt 
      aataaagct gcgattgcc agtgacatt tacagatta 
      tataaaacc gatcgattt tttgccaag ttatgacct 
      tttgcccaa catgggcgg aattatgaa cttgggatg 
      gggccaact taacgacga aacggctgg aatcaccga 
      tgagctcca ttatgtcta tttaattcc aatttctaa 
      tgaagagtg ccaactcag ctttggtag tcaaatttt 
      ggacacgat ggtgtgtgc tggtggtaa ttttaacga 
      aggtttttg gaaggtttg ccacaacaa cactggttt 
      tgtttgaag tttgcaaat cttttcagg gtgatagtg 
      gaacgccct tggtacgat atagtaacg gacctcgaa 
      ctacccatg tgggtgttg ccagtttta ttagcaaaa 
      atggatgcg atacgcctg agccatcgg gttatacac 
      gaacatatc cgtttgtag actggatac gaaatgtta 
      ccaacatag agtaaagaa aatattatg ctgttacga 
      aagaaacac atgttgaaa taaaagtat tcagccagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY