Status
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Gene information
General information
TC033012
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010906,TC010907
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1521986..1527854
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033012
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08195

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033012 RA
      MLYFAILCC ATLALGVPL QEAKSVQIG GRIIGGQKA 
      YAGQFPFLA AIYTHTKDG SYFCGGALL NQEWVLTAG 
      HCVDGAVSF TVHLGSNTL DGSDPNLIK LSTDTFVLH 
      PEYDPMTLN NDIGLIKFR MAITYSTYV YPIHMLPSA 
      PLSDYSPLL TMGWGQISD DDSGLQNDV HYVSVTTIS 
      NNECKIVYG NQIVETMAC AEGNYNEGI CYGDTGGPL 
      IQYVSRNQV MHVGVASFF SQNGCESTD PSGYTRTYN 
      YAKWIRNIT VEEKPSNKM KVLILAVVL SSALASPSL 
      RIIGGHDAN AGEFPYAAA IYVQTSDGT YFCAGALLS 
      NQWVLTSGS CVDGAVLFS IRVGANALN SSSSVKLTT 
      DNSVLHPDY STNPRDNDL GLIKLRLPV AFNDYVNAA 
      SLPTRDVQP YALITAIGW GQTSDDHAG LNNDLQAVT 
      LTALSNDEC KLAFGSQIN ANMVCVDGN YNQGTCLGD 
      TGGPLIQHV SGHIATVVG VSSFISAKG CESTDPSGY 
      FRTFPYVDW IKNVTGI
    CDS sequence
    • >TC033012 RA
      atgctttac tttgcgatt ttatgttgt gcaactctt 
      gcacttggg gttcccctt caggaggca aaatcggtc 
      caaatcgga ggaagaata attggtggt caaaaggca 
      tacgctgga cagttcccc tttctcgct gctatttat 
      acccataca aaggatgga agttatttt tgtgggggc 
      gcccttctg aaccaagaa tgggtcctg accgctggg 
      cattgcgtt gatggagct gtttcgttt actgtccat 
      ttggggtcc aacactttg gacggcagc gacccaaat 
      cttatcaaa ctatcaacc gacactttt gtgttgcat 
      cctgaatat gacccaatg actttgaac aatgacatt 
      ggtttgatc aaatttcgc atggctatc acatattcc 
      acttatgtg taccctata cacatgtta ccctcagct 
      ccgctatct gattattct ccacttttg acaatgggt 
      tgggggcag attagtgac gacgactct ggtcttcaa 
      aatgacgta cattacgtc tcggttaca accatttcg 
      aataatgag tgtaaaata gtttatgga aaccaaatt 
      gttgagacg atggcttgt gccgaagga aattataat 
      gaaggaatt tgctacggc gatactgga ggtccattg 
      attcaatat gtaagtcga aaccaagtt atgcacgtt 
      ggtgttgcc agtttcttt agccagaat ggatgcgaa 
      agcactgat ccttcagga tataccaga acgtacaat 
      tatgccaaa tggatcaga aatatcaca gttgaagaa 
      aagcctagt aacaaaatg aaggttctt attttggca 
      gttgtgcta tcctcggct ctcgcctcg ccaagcttg 
      cgtatcatc ggaggacac gatgcaaac gctggtgaa 
      tttccctat gcggcagca atttatgtt caaacttcg 
      gatggaact tacttttgc gctggggct ctattaagt 
      aaccaatgg gttttgacc agcggaagc tgtgttgat 
      ggggctgtg ttattctca attcgagtc ggagcgaat 
      gctctcaat tcgtcaagt tctgtgaaa cttacgact 
      gataattcc gtcttgcat ccagattat agcacaaat 
      ccgcgagat aatgatctt gggcttatt aagcttcgc 
      ttacctgtt gcttttaat gattacgta aacgcagct 
      tcgcttccc accagagac gtgcaaccc tatgccctt 
      attacggca attggttgg ggacaaacc agtgatgat 
      catgctgga cttaacaac gacctacaa gctgtcact 
      ttaacagct ttgtcgaat gacgaatgc aaacttgct 
      ttcggtagt caaattaac gcaaacatg gtctgtgtc 
      gatggaaat tataatcaa ggaacttgc ttgggtgat 
      actggaggc ccattgatt cagcatgtc agtggacat 
      attgccaca gtggttgga gtctctagt ttcattagc 
      gcaaaaggg tgcgaaagt actgatccc tcggggtat 
      ttcaggact tttccctac gttgattgg attaaaaat 
      gtaacgggg atatag
    mRNA sequence
    • >TC033012 RA
      tgttttgcg tcaaaatgc tttactttg cgattttat 
      gttgtgcaa ctcttgcac ttggggttc cccttcagg 
      aggcaaaat cggtccaaa tcggaggaa gaataattg 
      gtggtcaaa aggcatacg ctggacagt tcccctttc 
      tcgctgcta tttataccc atacaaagg atggaagtt 
      atttttgtg ggggcgccc ttctgaacc aagaatggg 
      tcctgaccg ctgggcatt gcgttgatg gagctgttt 
      cgtttactg tccatttgg ggtccaaca ctttggacg 
      gcagcgacc caaatctta tcaaactat caaccgaca 
      cttttgtgt tgcatcctg aatatgacc caatgactt 
      tgaacaatg acattggtt tgatcaaat ttcgcatgg 
      ctatcacat attccactt atgtgtacc ctatacaca 
      tgttaccct cagctccgc tatctgatt attctccac 
      ttttgacaa tgggttggg ggcagatta gtgacgacg 
      actctggtc ttcaaaatg acgtacatt acgtctcgg 
      ttacaacca tttcgaata atgagtgta aaatagttt 
      atggaaacc aaattgttg agacgatgg cttgtgccg 
      aaggaaatt ataatgaag gaatttgct acggcgata 
      ctggaggtc cattgattc aatatgtaa gtcgaaacc 
      aagttatgc acgttggtg ttgccagtt tctttagcc 
      agaatggat gcgaaagca ctgatcctt caggatata 
      ccagaacgt acaattatg ccaaatgga tcagaaata 
      tcacagttg aagaaaagc ctagtaaca aaatgaagg 
      ttcttattt tggcagttg tgctatcct cggctctcg 
      cctcgccaa gcttgcgta tcatcggag gacacgatg 
      caaacgctg gtgaatttc cctatgcgg cagcaattt 
      atgttcaaa cttcggatg gaacttact tttgcgctg 
      gggctctat taagtaacc aatgggttt tgaccagcg 
      gaagctgtg ttgatgggg ctgtgttat tctcaattc 
      gagtcggag cgaatgctc tcaattcgt caagttctg 
      tgaaactta cgactgata attccgtct tgcatccag 
      attatagca caaatccgc gagataatg atcttgggc 
      ttattaagc ttcgcttac ctgttgctt ttaatgatt 
      acgtaaacg cagcttcgc ttcccacca gagacgtgc 
      aaccctatg cccttatta cggcaattg gttggggac 
      aaaccagtg atgatcatg ctggactta acaacgacc 
      tacaagctg tcactttaa cagctttgt cgaatgacg 
      aatgcaaac ttgctttcg gtagtcaaa ttaacgcaa 
      acatggtct gtgtcgatg gaaattata atcaaggaa 
      cttgcttgg gtgatactg gaggcccat tgattcagc 
      atgtcagtg gacatattg ccacagtgg ttggagtct 
      ctagtttca ttagcgcaa aagggtgcg aaagtactg 
      atccctcgg ggtatttca ggacttttc cctacgttg 
      attggatta aaaatgtaa cggggatat agatgggtt 
      ttaggattt ataaaaaaa taattaaaa gtttggtta 
      ttggtgttt aataatttt ctaaataat agaatgtaa 
      attttactt gagtgagtt tttaataaa gtgatgaag 
      cgttc
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