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Gene information
General information
TC016317 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 999660..1000980
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016317
    iBeetle screen iB_08191

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late structure degenerated - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016317 RA
      MSTKLQIRR QKWALDNTR NRSVAEGLA 
      APPAFSPSG PASSSDVSK KTDVSRLIT 
      KKSWDLALA PLKQVPMNL FIMYMAGNS 
      ISIFPIMMV GMLLMRPVQ AIFATKSTF 
      KMVENSSAF KQMFVYILG NCINIGLAL 
      YKCQSMGLL PTHSSDWLA FVEPPVRLE 
      YLGGGVSLL
    CDS sequence
    • >TC016317 RA
      atgtctacg aaacttcag atcaggcgg caaaagtgg 
      gctttggac aataccaga aatcgcagt gtagcagaa 
      ggtttggcc gctccccca gcctttagc ccctctgga 
      cctgcctca agcagtgat gtttcgaaa aaaacggac 
      gttagtcgc ctaataact aaaaaatcg tgggacctg 
      gctttagcc cccttgaag caagttcca atgaacttg 
      ttcatcatg tacatggca gggaattca atttcaatt 
      ttcccaatt atgatggtt gggatgctt ctgatgagg 
      ccagttcag gccattttc gccacaaaa agtacattc 
      aaaatggtg gaaaatagc agtgctttt aaacaaatg 
      tttgtctac attttgggg aattgcatt aatattggt 
      ttagccctt tacaagtgt caaagtatg ggcttgttg 
      cccacacat tccagcgac tggttggcc tttgtggag 
      cccccagtc agacttgaa tatttgggt ggtggtgta 
      tcactatta taa
    mRNA sequence
    • >TC016317 RA
      gtggcagtg tctgaggtg tgacttgtg ttttgttat 
      tcttgtcaa acttaatcg ttaagtggt aattactga 
      tggtttggt gcttcagga aatgactac gtctcggtt 
      aattcaatc ccggaccta atctctttt attcaaaac 
      tcgtttgaa tttttttag tacgcgtgt cgttaccgc 
      aatcgatag tggttacag ttacgacag tcggacata 
      acctgaaat attaataag tttgtgtca ataattgtg 
      tcaaaattg cagcaggat gtctacgaa acttcagat 
      caggcggca aaagtgggc tttggacaa taccagaaa 
      tcgcagtgt agcagaagg tttggccgc tcccccagc 
      ctttagccc ctctggacc tgcctcaag cagtgatgt 
      ttcgaaaaa aacggacgt tagtcgcct aataactaa 
      aaaatcgtg ggacctggc tttagcccc cttgaagca 
      agttccaat gaacttgtt catcatgta catggcagg 
      gaattcaat ttcaatttt cccaattat gatggttgg 
      gatgcttct gatgaggcc agttcaggc cattttcgc 
      cacaaaaag tacattcaa aatggtgga aaatagcag 
      tgcttttaa acaaatgtt tgtctacat tttggggaa 
      ttgcattaa tattggttt agcccttta caagtgtca 
      aagtatggg cttgttgcc cacacattc cagcgactg 
      gttggcctt tgtggagcc cccagtcag acttgaata 
      tttgggtgg tggtgtatc actattata atgtaatag 
      cataatttt atttaaata aatcacaca cagctacta 
      aacgattaa tctattgct tcctatgtt tatcggcaa 
      agtctttaa tcttttcaa ataaagcct gagggtcgt 
      ccactcgcg gaatctgca aaatttcaa taatcaata 
      gaactattg tgtaaatca acaaaaaat acttacatc 
      atagttttg gctaaggta aactcctgc ataaagtcc 
      cacgataaa actgaactg gaattttaa aaataaaaa 
      ttggcacaa ccaacgaat ggttgcgaa tgaatattt 
      accaatcct ctaaccata tttctagtt tgttttggg 
      cctattatg ttaatttat tccgttctt tgttaactt 
      aagtataaa ttgttcgtc agcttgaca ttgaaattt 
      tgtgacagc tgccatgtg acagcctgg gtcgccaat 
      ccaagaaca tcaaactca ccaagtcca ctaaatgaa 
      taatttttt ttgtagctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY