Status
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Gene information
General information
TC032982
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005145,TC005146
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450690.1: 44..5236
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032982
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_08189

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032982 RA
        MTDDLKLYD ALVKVSDQY DSVIFGDFN MPDIVWSRV 
        SPQATKPSS QLFLDILSD SRVSQIISE PTRFRVNQS 
        PSVLDLILT TDESLVSGL EYLSPLGKS DHTVLKVDL 
        QLCFVAVGR VNRQTKLST NFDELNEKL SKINWNAVL 
        LNLSTECMW ESFKDTVYS LVKSCSRKI VKKQSSSKP 
        WINSKILRQ INKKRALWR AYRRTGSIV DYQNHRAFS 
        NNLTKIIRD ARARYERNI INSADSKRL FKYIRTKLN 
        GPVTIPKLR DASGDICEN DITNANMFA DKFCEVFTD 
        EPSISIPCP LTAPNESCI DNIFITEAK LRDKLNKLK 
        VTKSPGPDG LSPLIIKKC SSSLTKPLC SIMNHSLAS 
        GELPNDWKT AIVKPIFKR GDKFDPLNY RPISLTSVI 
        VKILESLIF DKLIDFLVT TNRIPEEQH GFVPGRSIV 
        SNLLCCLED WTNIIDANK AADIIYLDF SKAFDRVPR 
        RRLLYKLKH NGINGSLLN WIESFLSNR TFKVCVNNS 
        LSTSRQVMS GVPQGSVLA RRIILSNVC PSIPHPIVE 
        EMVKRIGFT LVSKMSFLK AALSHEEYK HVLSFRRQV 
        YVQPEDSFD LPSSLVLKY EDTNYRIFL NFDDVCFKC 
        RQLGHFAQD CPNVSHTDV GSKRPPTDD ISPIPDCVR 
        SSDSHAEDD DDLDTPHPK KKKSCDSTE SLTPTPELL 
        QPIRELVKN AQYPLSFSE LIELMEKLV GENDILNLV 
        SKYTDDIEG LLAMLFDIF PLVEHRSMQ NRIKRTRKR 
        IKKAYENLP RDRVPAPYT GMETEPIMY TNIASLTAK 
        FHELSVLVK STHPALFLI SETWLTPTL SDSIVALSG 
        LYRKDRLHK RGGGVCVYV ADDIAESFI ITPLQFDLG 
        AIDSIFLKI TNSNCALVH TCIYRPPNC DMTDDLKLY 
        DALVKVSDH MEPSFSSGY QAILPALFG YPF
      CDS sequence
      • >TC032982 RA
        atgaccgat gacttgaag ctatatgat gccttagtt 
        aaggtctcc gaccagtat gattctgtc atatttggt 
        gattttaac atgccagat atagtatgg agccgagtt 
        tctcctcag gctaccaag ccatcctcc cagctcttt 
        ttggatatc ctttctgat tctcgggtt tctcaaata 
        atatcggaa cctacgcgc tttcgggtc aatcaatca 
        ccttcggtg cttgacctt atactgact actgacgaa 
        tcactcgtc agtggtttg gaatatctc agtcctctt 
        ggaaaatcc gaccatacc gtactgaag gttgatctt 
        cagctgtgt tttgttgca gttggccgg gtcaaccgt 
        caaactaaa ctgtctacc aacttcgac gagttgaat 
        gagaaactc agtaagatt aattggaat gcagtgctt 
        ttgaatctg tccactgaa tgcatgtgg gaaagtttc 
        aaagatacg gtatactcc ttagtaaag agctgttca 
        cgtaaaatt gtgaagaaa caatcctcg tcgaagcct 
        tggataaac agtaaaata ttgaggcaa ataaataaa 
        aagagagcg ctttggaga gcttatagg cgcaccgga 
        agtatagta gactatcaa aatcatagg gctttttca 
        aacaatttg acgaagatc attagagat gctagggca 
        cgttacgag agaaatatt attaactct gccgattcc 
        aaaagacta tttaaatac atccgtacc aaactcaat 
        ggtcctgta accatacca aaacttaga gacgcgtcg 
        ggagacatt tgtgagaac gacattaca aatgccaac 
        atgtttgcc gataagttt tgcgaagtc tttactgat 
        gaaccttct atctcaatt ccttgccct cttactgca 
        cccaatgaa tcgtgtatc gataatata tttattacg 
        gaagccaag ctacgcgac aaattaaat aaacttaag 
        gtaactaaa tctcctggg cctgatggt ttaagtcct 
        ctgataatt aaaaaatgt tcttcgtca ctaaccaag 
        ccactttgt tctataatg aaccattct ttagcgtct 
        ggtgaattg ccaaatgat tggaagacc gccattgtt 
        aaaccaatc tttaaaagg ggtgacaaa tttgacccg 
        cttaattat cgtcccatt agtctcact tcggtcatt 
        gttaaaata cttgaatcg ctcattttt gataagtta 
        atagatttt ttagtcacc actaatagg attccagaa 
        gaacagcat ggatttgtt ccgggtcgt tcaattgtg 
        tcaaatctt ctttgttgt ttggaggat tggaccaat 
        attattgat gctaataaa gcagcagat atcatctat 
        ttagatttt tctaaagct ttcgatcgg gttccgaga 
        cgtaggctt ttgtacaaa ttaaaacat aatggaatc 
        aatggatca ctgcttaat tggattgag tcgtttctg 
        agtaacaga actttcaaa gtgtgtgtt aacaacagt 
        cttagcact tccaggcag gtgatgagc ggagtgccg 
        caaggctct gtattagca agacgtatt atcctatcc 
        aatgtgtgc ccgtctatt ccgcacccc attgttgaa 
        gaaatggtt aaaagaata ggatttacc ttagtctct 
        aaaatgtca tttctcaaa gcggctttg agccatgaa 
        gaatacaaa catgtttta agtttccgc agacaggtt 
        tacgttcaa ccagaagat tcctttgat ctcccctcc 
        tctcttgtt ctgaaatac gaggacacc aactatcgc 
        attttccta aattttgac gacgtctgc tttaaatgt 
        agacaactt gggcatttt gcccaagat tgccctaat 
        gtctcgcac accgacgtt ggatctaag cggccgccc 
        acagacgat atttcccct ataccagat tgcgtgaga 
        tcctctgat tcccatgca gaagatgat gacgacctt 
        gatacacca catcctaag aaaaaaaaa tcctgtgat 
        tcaacagaa agtctgacg cctacaccg gaattgttg 
        cagccaatc agggaatta gttaaaaat gcccagtac 
        ccgctaagt ttcagcgag ctgatagaa ctaatggaa 
        aagcttgtt ggtgagaac gatatactt aacttggtt 
        tcgaagtac actgacgac atcgaagga ttgctggct 
        atgctgttt gatattttt cccttggtt gaacatcga 
        agcatgcag aatagaatt aagagaacg cgcaaaaga 
        attaaaaaa gcttatgag aatttaccg cgtgaccga 
        gtgcccgcc ccatacact ggtatggag acggaacct 
        ataatgtat acaaacatc gcttcacta acagccaaa 
        tttcacgaa ttgtcagtg ctagttaaa tccacccat 
        cccgctctc tttcttatt tcggaaacg tggttgact 
        cctacttta agcgattct atcgtagct ctcagcggc 
        ctttatcgt aaggacaga ttacacaaa agaggaggg 
        ggagtgtgt gtttatgtt gccgatgat atcgctgaa 
        agttttata atcacccct ctgcagttt gatctgggt 
        gcaattgac tcgattttc ctcaaaatt actaatagc 
        aattgtgcc ttagttcat acatgtata tatagaccg 
        cctaactgt gatatgacc gatgacttg aagctatat 
        gatgcatta gttaaggtc tccgaccat atggagccg 
        agtttctcc tcaggctat caagccatc ctcccagct 
        ctttttgga tatcctttc tga
      mRNA sequence
      • >TC032982 RA
        gagcgtgtg tttatgttg ccgatgata tcgctgaaa 
        gttttataa tcacccctc tgcagtatg atctgggtg 
        caattgact cgatttttc tcaaaatta ctaatagca 
        attgtactt tagttctta catgtatat atagaccgc 
        ctaactgtg atatgaccg atgacttga agctatatg 
        atgccttag ttaaggtct ccgaccagt atgattctg 
        tcatatttg gtgatttta acatgccag atatagtat 
        ggagccgag tttctcctc aggctacca agccatcct 
        cccagctct ttttggata tcctttctg attctcggg 
        tttctcaaa taatatcgg aacctacgc gctttcggg 
        tcaatcaat caccttcgg tgcttgacc ttatactga 
        ctactgacg aatcactcg tcagtggtt tggaatatc 
        tcagtcctc ttggaaaat ccgaccata ccgtactga 
        aggttgatc ttcagctgt gttttgttg cagttggcc 
        gggtcaacc gtcaaacta aactgtcta ccaacttcg 
        acgagttga atgagaaac tcagtaaga ttaattgga 
        atgcagtgc ttttgaatc tgtccactg aatgcatgt 
        gggaaagtt tcaaagata cggtatact ccttagtaa 
        agagctgtt cacgtaaaa ttgtgaaga aacaatcct 
        cgtcgaagc cttggataa acagtaaaa tattgaggc 
        aaataaata aaaagagag cgctttgga gagcttata 
        ggcgcaccg gaagtatag tagactatc aaaatcata 
        gggcttttt caaacaatt tgacgaaga tcattagag 
        atgctaggg cacgttacg agagaaata ttattaact 
        ctgccgatt ccaaaagac tatttaaat acatccgta 
        ccaaactca atggtcctg taaccatac caaaactta 
        gagacgcgt cgggagaca tttgtgaga acgacatta 
        caaatgcca acatgtttg ccgataagt tttgcgaag 
        tctttactg atgaacctt ctatctcaa ttccttgcc 
        ctcttactg cacccaatg aatcgtgta tcgataata 
        tatttatta cggaagcca agctacgcg acaaattaa 
        ataaactta aggtaacta aatctcctg ggcctgatg 
        gtttaagtc ctctgataa ttaaaaaat gttcttcgt 
        cactaacca agccacttt gttctataa tgaaccatt 
        ctttagcgt ctggtgaat tgccaaatg attggaaga 
        ccgccattg ttaaaccaa tctttaaaa ggggtgaca 
        aatttgacc cgcttaatt atcgtccca ttagtctca 
        cttcggtca ttgttaaaa tacttgaat cgctcattt 
        ttgataagt taatagatt ttttagtca ccactaata 
        ggattccag aagaacagc atggatttg ttccgggtc 
        gttcaattg tgtcaaatc ttctttgtt gtttggagg 
        attggacca atattattg atgctaata aagcagcag 
        atatcatct atttagatt tttctaaag ctttcgatc 
        gggttccga gacgtaggc ttttgtaca aattaaaac 
        ataatggaa tcaatggat cactgctta attggattg 
        agtcgtttc tgagtaaca gaactttca aagtgtgtg 
        ttaacaaca gtcttagca cttccaggc aggtgatga 
        gcggagtgc cgcaaggct ctgtattag caagacgta 
        ttatcctat ccaatgtgt gcccgtcta ttccgcacc 
        ccattgttg aagaaatgg ttaaaagaa taggattta 
        ccttagtct ctaaaatgt catttctca aagcggctt 
        tgagccatg aagaataca aacatgttt taagtttcc 
        gcagacagg tttacgttc aaccagaag attcctttg 
        atctcccct cctctcttg ttctgaaat acgaggaca 
        ccaactatc gcattttcc taaattttg acgacgtct 
        gctttaaat gtagacaac ttgggcatt ttgcccaag 
        attgcccta atgtctcgc acaccgacg ttggatcta 
        agcggccgc ccacagacg atatttccc ctataccag 
        attgcgtga gatcctctg attcccatg cagaagatg 
        atgacgacc ttgatacac cacatccta agaaaaaaa 
        aatcctgtg attcaacag aaagtctga cgcctacac 
        cggaattgt tgcagccaa tcagggaat tagttaaaa 
        atgcccagt acccgctaa gtttcagcg agctgatag 
        aactaatgg aaaagcttg ttggtgaga acgatatac 
        ttaacttgg tttcgaagt acactgacg acatcgaag 
        gattgctgg ctatgctgt ttgatattt ttcccttgg 
        ttgaacatc gaagcatgc agaatagaa ttaagagaa 
        cgcgcaaaa gaattaaaa aagcttatg agaatttac 
        cgcgtgacc gagtgcccg ccccataca ctggtatgg 
        agacggaac ctataatgt atacaaaca tcgcttcac 
        taacagcca aatttcacg aattgtcag tgctagtta 
        aatccaccc atcccgctc tctttctta tttcggaaa 
        cgtggttga ctcctactt taagcgatt ctatcgtag 
        ctctcagcg gcctttatc gtaaggaca gattacaca 
        aaagaggag ggggagtgt gtgtttatg ttgccgatg 
        atatcgctg aaagtttta taatcaccc ctctgcagt 
        ttgatctgg gtgcaattg actcgattt tcctcaaaa 
        ttactaata gcaattgtg ccttagttc atacatgta 
        tatatagac cgcctaact gtgatatga ccgatgact 
        tgaagctat atgatgcat tagttaagg tctccgacc 
        atatggagc cgagtttct cctcaggct atcaagcca 
        tcctcccag ctctttttg gatatcctt tctgattct 
        cggatctct caaataata tcggaacct acgcgcttt 
        cgggtcaat caatcacct tcggtgctt gaccttata 
        ttgactact gacgaatca cttgtcagt ggtttggaa 
        tatctcagt cctcttgga aaatccgac cataccgtg 
        ctgaaggtt gatcttcag ctgtgtttt gttgcagtt 
        ggccgggtc aaccgtcaa actaaactg tctaccaac 
        tt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY