Status
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Gene information
General information
TC008383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9584233..9590168
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008383
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08179

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands unilateral colour darker - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection

    Comment: preparation insufficient

    abdominal odoriferous glands partially colour lighter - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008383 RA
      MAQKVSQNK TYIAVNIPE NSNKAVRKS FWSVFKRDA 
STKKSKEAP KEVAPATPR INKADLARL LSLAKPEKW 
KLFGAICFL LVSSTVTMS IPFSLGKVL DIIYSSSEN 
SDEARAKLN KVCGVLLCV FVLGAICNF GRVYLMSVS 
GYRMTQALR KRVFSTIMK QEQGWFDKR STGELVNRL 
SADTQIVGN ALSSNISDG LRSIVMVCA GTGMMFYMS 
AELALVGLA IVPPVAIIA VIYGRFLRG ISRKVQDSL 
AGATSVAEE RIANIRTVK TFSQEPREV DSYANAIKE 
VLGLSYREA KARAIFYGL TGFSGHVII ISVLYYGGV 
MVSSNTITV GNLSSFLLY AAYIGVSLG TFSNFYSEL 
NKSIGAAAR IWEIIDREP IIPMKGGLV PIAPPEGHI 
QFNDIQFSY PARDDVRIF ENFKLEISP GKMVAVVGP 
SGSGKSTLA ALLLRLYDP AKGQVLLDG QDIKEIDPS 
WLRRHVGTV SQEPVLFSC SIRDNILYG ADDPSKVTE 
DELVQVAKE ANVYEFVQQ LPDGFDTLV GERGVMLSG 
GQKQRIAIA RALIKDPKI LLLDEATSA LDAQSEHLV 
QEALERIMK GRTVLTIAH RLSTIQNAD TIAVLRDGH 
IVEQGKYQD LLNIKKGAF RELMKHQTF QGNIEKT
    CDS sequence
    • >TC008383 RA
      atggcccag aaagtgagt cagaacaag acatacatc 
gctgtgaat atccccgaa aattcgaat aaagcagtt 
cgaaaatca ttttggtcg gtttttaaa agggacgct 
agcacaaag aaaagcaaa gaagcgccc aaagaagta 
gccccagcg accccgcgc attaacaaa gctgatttg 
gcccgcctg ctgtcctta gccaagcca gaaaaatgg 
aaacttttc ggagcgatt tgtttcttg ctggtgtca 
agcaccgtc acaatgagc atccctttt agtttaggc 
aaagtcctt gacatcatt tatagtagt agtgaaaat 
agtgatgag gctcgagcg aaattaaat aaagtttgc 
ggtgtttta ttgtgcgtg tttgtctta ggggcgatt 
tgcaatttt ggacgtgtg tacctaatg tcagtatca 
ggttataga atgactcaa gctttaaga aaacgtgtt 
ttttctacc ataatgaag caggaacaa ggctggttt 
gataagaga tcaactggg gagttggta aacagactc 
tctgccgat actcaaatt gtgggaaat gctctctct 
agcaatatt tcagacggg ctaaggtca attgtcatg 
gtctgtgct ggtacaggc atgatgttt tatatgtcg 
gcagaactg gcacttgtt ggcttggct attgtgccc 
ccggttgcg attattgcc gttatttat gggagattc 
cttagaggg atttctagg aaggttcag gattcttta 
gcaggggct actagtgtt gcagaggaa cgtattgct 
aatatcagg actgttaaa actttctcg caggaaccg 
agagaagtt gattcgtac gcaaatgcc attaaagag 
gttctaggc ttgagttac agagaggct aaagctagg 
gccattttt tatgggctg accggcttt agcggccac 
gtaatcata atatcagtg ctttattat ggtggtgtg 
atggtgtct tcaaacacc ataacagtg ggaaatctg 
tcttctttt ctcttatat gcggcttat atcggcgta 
tcgttgggc acattttca aatttttac tccgaattg 
aacaaatca attggtgct gcggccaga atttgggaa 
attatcgac agagaaccc ataattccg atgaaaggt 
ggtttagtg ccaattgca ccccctgaa ggtcacata 
caatttaac gacatccag tttagttac ccagcgcgc 
gacgatgtc cgcattttc gaaaatttc aaactcgaa 
atatccccc ggaaaaatg gttgcagta gtcggtccg 
agtggttcc ggcaaaagc accctcgct gctctttta 
ttgcgacta tacgatcct gcaaaagga caagttcta 
ttagacgga caggacatt aaagaaatt gatccgagt 
tggttgcgc cgacatgtc ggtaccgtt agccaggaa 
ccagtttta ttttcgtgt tcaattcgt gataatatt 
ttatacgga gctgatgat ccttccaaa gtgacagaa 
gatgaatta gttcaagtg gccaaagag gccaatgtt 
tacgaattc gtccaacag ttgcccgac ggttttgat 
actttggta ggggaacgt ggggtgatg ttgagcggt 
ggccagaaa caacgcatt gccatagcg agagctttg 
attaaagat ccgaagata ttgttgtta gatgaagcc 
acgagtgcg ttagatgcg caatcggag cacttggtg 
caagaagct ttggagagg attatgaaa gggaggact 
gttttaacg attgctcat aggctgtcg acgattcaa 
aatgccgat actattgct gtgctgcgc gatggacat 
atcgtggaa caggggaaa taccaggat ttacttaat 
atcaagaag ggcgcgttt agggaatta atgaaacat 
caaacgttt caagggaat atagagaag acgtga
    mRNA sequence
    • >TC008383 RA
      cgcaataag catgttacc tcaaaaact gatgattat 
tagagtaaa tatacgtct ttatcaatg ttttagtgc 
aagcaagtt aaattcaag ttaaaaatt aattagagc 
gctgatcga tttatcgaa aactttcag tttcagcgc 
ttcagtata gtcggaact ttacgcgag tttgtgtaa 
gtttttttc tttggaatt tttggaaaa taatcaagg 
cacgtcgta ctttatcta cctatttct tgaattaac 
tttctgttt aaataatct tttctaaat ccctgttac 
tcgagcgaa taaggtcat aaacctcat caaggataa 
tgataacat ttcatagtc cgcccctct cccgcttta 
tcacatttt ctcaaacgt cattcctgt catgtttta 
gtgtcctaa cctagaaaa cgtgtcgaa taaaacctt 
gttgcgtaa attaacacc acaatgacg ctttaaatt 
aacataaaa gtgaactgt attttatct ttatttaac 
caaaaccaa gtaaattca tagtaaaaa tgaatatta 
tttgtttac tagtgagcc gacataacc tcaaaaact 
cactgaaat gtttaagac acgttaaca accgctttt 
tcctccaaa acaaacttt ccgttcgaa atcacaaga 
cgatggccc agaaagtga gtcagaaca agacataca 
tcgctgtga atatccccg aaaattcga ataaagcag 
ttcgaaaat cattttggt cggttttta aaagggacg 
ctagcacaa agaaaagca aagaagcgc ccaaagaag 
tagccccag cgaccccgc gcattaaca aagctgatt 
tggcccgcc tgctgtcct tagccaagc cagaaaaat 
ggaaacttt tcggagcga tttgtttct tgctggtgt 
caagcaccg tcacaatga gcatccctt ttagtttag 
gcaaagtcc ttgacatca tttatagta gtagtgaaa 
atagtgatg aggctcgag cgaaattaa ataaagttt 
gcggtgttt tattgtgcg tgtttgtct taggggcga 
tttgcaatt ttggacgtg tgtacctaa tgtcagtat 
caggttata gaatgactc aagctttaa gaaaacgtg 
ttttttcta ccataatga agcaggaac aaggctggt 
ttgataaga gatcaactg gggagttgg taaacagac 
tctctgccg atactcaaa ttgtgggaa atgctctct 
ctagcaata tttcagacg ggctaaggt caattgtca 
tggtctgtg ctggtacag gcatgatgt tttatatgt 
cggcagaac tggcacttg ttggcttgg ctattgtgc 
ccccggttg cgattattg ccgttattt atgggagat 
tccttagag ggatttcta ggaaggttc aggattctt 
tagcagggg ctactagtg ttgcagagg aacgtattg 
ctaatatca ggactgtta aaactttct cgcaggaac 
cgagagaag ttgattcgt acgcaaatg ccattaaag 
aggttctag gcttgagtt acagagagg ctaaagcta 
gggccattt tttatgggc tgaccggct ttagcggcc 
acgtaatca taatatcag tgctttatt atggtggtg 
tgatggtgt cttcaaaca ccataacag tgggaaatc 
tgtcttctt ttctcttat atgcggctt atatcggcg 
tatcgttgg gcacatttt caaattttt actccgaat 
tgaacaaat caattggtg ctgcggcca gaatttggg 
aaattatcg acagagaac ccataattc cgatgaaag 
gtggtttag tgccaattg caccccctg aaggtcaca 
tacaattta acgacatcc agtttagtt acccagcgc 
gcgacgatg tccgcattt tcgaaaatt tcaaactcg 
aaatatccc ccggaaaaa tggttgcag tagtcggtc 
cgagtggtt ccggcaaaa gcaccctcg ctgctcttt 
tattgcgac tatacgatc ctgcaaaag gacaagttc 
tattagacg gacaggaca ttaaagaaa ttgatccga 
gttggttgc gccgacatg tcggtaccg ttagccagg 
aaccagttt tattttcgt gttcaattc gtgataata 
ttttatacg gagctgatg atccttcca aagtgacag 
aagatgaat tagttcaag tggccaaag aggccaatg 
tttacgaat tcgtccaac agttgcccg acggttttg 
atactttgg taggggaac gtggggtga tgttgagcg 
gtggccaga aacaacgca ttgccatag cgagagctt 
tgattaaag atccgaaga tattgttgt tagatgaag 
ccacgagtg cgttagatg cgcaatcgg agcacttgg 
tgcaagaag ctttggaga ggattatga aagggagga 
ctgttttaa cgattgctc ataggctgt cgacgattc 
aaaatgccg atactattg ctgtgctgc gcgatggac 
atatcgtgg aacagggga aataccagg atttactta 
atatcaaga agggcgcgt ttagggaat taatgaaac 
atcaaacgt ttcaaggga atatagaga agacgtgat 
gtacttaat ttattttat ttttacact tttgaggac 
ttgttacac gtttatttg ttatgcaaa taaaatagt 
tttactgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY