General information | |||
TC008383 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9584233..9590168 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC008383 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08179 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Comment: preparation insufficient |
abdominal odoriferous glands partially colour lighter - Number of animals showing the phenotype: 2 (13 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC008383 RA
MAQKVSQNK TYIAVNIPE NSNKAVRKS FWSVFKRDA STKKSKEAP KEVAPATPR INKADLARL LSLAKPEKW KLFGAICFL LVSSTVTMS IPFSLGKVL DIIYSSSEN SDEARAKLN KVCGVLLCV FVLGAICNF GRVYLMSVS GYRMTQALR KRVFSTIMK QEQGWFDKR STGELVNRL SADTQIVGN ALSSNISDG LRSIVMVCA GTGMMFYMS AELALVGLA IVPPVAIIA VIYGRFLRG ISRKVQDSL AGATSVAEE RIANIRTVK TFSQEPREV DSYANAIKE VLGLSYREA KARAIFYGL TGFSGHVII ISVLYYGGV MVSSNTITV GNLSSFLLY AAYIGVSLG TFSNFYSEL NKSIGAAAR IWEIIDREP IIPMKGGLV PIAPPEGHI QFNDIQFSY PARDDVRIF ENFKLEISP GKMVAVVGP SGSGKSTLA ALLLRLYDP AKGQVLLDG QDIKEIDPS WLRRHVGTV SQEPVLFSC SIRDNILYG ADDPSKVTE DELVQVAKE ANVYEFVQQ LPDGFDTLV GERGVMLSG GQKQRIAIA RALIKDPKI LLLDEATSA LDAQSEHLV QEALERIMK GRTVLTIAH RLSTIQNAD TIAVLRDGH IVEQGKYQD LLNIKKGAF RELMKHQTF QGNIEKT
- >TC008383 RA
atggcccag aaagtgagt cagaacaag acatacatc gctgtgaat atccccgaa aattcgaat aaagcagtt cgaaaatca ttttggtcg gtttttaaa agggacgct agcacaaag aaaagcaaa gaagcgccc aaagaagta gccccagcg accccgcgc attaacaaa gctgatttg gcccgcctg ctgtcctta gccaagcca gaaaaatgg aaacttttc ggagcgatt tgtttcttg ctggtgtca agcaccgtc acaatgagc atccctttt agtttaggc aaagtcctt gacatcatt tatagtagt agtgaaaat agtgatgag gctcgagcg aaattaaat aaagtttgc ggtgtttta ttgtgcgtg tttgtctta ggggcgatt tgcaatttt ggacgtgtg tacctaatg tcagtatca ggttataga atgactcaa gctttaaga aaacgtgtt ttttctacc ataatgaag caggaacaa ggctggttt gataagaga tcaactggg gagttggta aacagactc tctgccgat actcaaatt gtgggaaat gctctctct agcaatatt tcagacggg ctaaggtca attgtcatg gtctgtgct ggtacaggc atgatgttt tatatgtcg gcagaactg gcacttgtt ggcttggct attgtgccc ccggttgcg attattgcc gttatttat gggagattc cttagaggg atttctagg aaggttcag gattcttta gcaggggct actagtgtt gcagaggaa cgtattgct aatatcagg actgttaaa actttctcg caggaaccg agagaagtt gattcgtac gcaaatgcc attaaagag gttctaggc ttgagttac agagaggct aaagctagg gccattttt tatgggctg accggcttt agcggccac gtaatcata atatcagtg ctttattat ggtggtgtg atggtgtct tcaaacacc ataacagtg ggaaatctg tcttctttt ctcttatat gcggcttat atcggcgta tcgttgggc acattttca aatttttac tccgaattg aacaaatca attggtgct gcggccaga atttgggaa attatcgac agagaaccc ataattccg atgaaaggt ggtttagtg ccaattgca ccccctgaa ggtcacata caatttaac gacatccag tttagttac ccagcgcgc gacgatgtc cgcattttc gaaaatttc aaactcgaa atatccccc ggaaaaatg gttgcagta gtcggtccg agtggttcc ggcaaaagc accctcgct gctctttta ttgcgacta tacgatcct gcaaaagga caagttcta ttagacgga caggacatt aaagaaatt gatccgagt tggttgcgc cgacatgtc ggtaccgtt agccaggaa ccagtttta ttttcgtgt tcaattcgt gataatatt ttatacgga gctgatgat ccttccaaa gtgacagaa gatgaatta gttcaagtg gccaaagag gccaatgtt tacgaattc gtccaacag ttgcccgac ggttttgat actttggta ggggaacgt ggggtgatg ttgagcggt ggccagaaa caacgcatt gccatagcg agagctttg attaaagat ccgaagata ttgttgtta gatgaagcc acgagtgcg ttagatgcg caatcggag cacttggtg caagaagct ttggagagg attatgaaa gggaggact gttttaacg attgctcat aggctgtcg acgattcaa aatgccgat actattgct gtgctgcgc gatggacat atcgtggaa caggggaaa taccaggat ttacttaat atcaagaag ggcgcgttt agggaatta atgaaacat caaacgttt caagggaat atagagaag acgtga
- >TC008383 RA
cgcaataag catgttacc tcaaaaact gatgattat tagagtaaa tatacgtct ttatcaatg ttttagtgc aagcaagtt aaattcaag ttaaaaatt aattagagc gctgatcga tttatcgaa aactttcag tttcagcgc ttcagtata gtcggaact ttacgcgag tttgtgtaa gtttttttc tttggaatt tttggaaaa taatcaagg cacgtcgta ctttatcta cctatttct tgaattaac tttctgttt aaataatct tttctaaat ccctgttac tcgagcgaa taaggtcat aaacctcat caaggataa tgataacat ttcatagtc cgcccctct cccgcttta tcacatttt ctcaaacgt cattcctgt catgtttta gtgtcctaa cctagaaaa cgtgtcgaa taaaacctt gttgcgtaa attaacacc acaatgacg ctttaaatt aacataaaa gtgaactgt attttatct ttatttaac caaaaccaa gtaaattca tagtaaaaa tgaatatta tttgtttac tagtgagcc gacataacc tcaaaaact cactgaaat gtttaagac acgttaaca accgctttt tcctccaaa acaaacttt ccgttcgaa atcacaaga cgatggccc agaaagtga gtcagaaca agacataca tcgctgtga atatccccg aaaattcga ataaagcag ttcgaaaat cattttggt cggttttta aaagggacg ctagcacaa agaaaagca aagaagcgc ccaaagaag tagccccag cgaccccgc gcattaaca aagctgatt tggcccgcc tgctgtcct tagccaagc cagaaaaat ggaaacttt tcggagcga tttgtttct tgctggtgt caagcaccg tcacaatga gcatccctt ttagtttag gcaaagtcc ttgacatca tttatagta gtagtgaaa atagtgatg aggctcgag cgaaattaa ataaagttt gcggtgttt tattgtgcg tgtttgtct taggggcga tttgcaatt ttggacgtg tgtacctaa tgtcagtat caggttata gaatgactc aagctttaa gaaaacgtg ttttttcta ccataatga agcaggaac aaggctggt ttgataaga gatcaactg gggagttgg taaacagac tctctgccg atactcaaa ttgtgggaa atgctctct ctagcaata tttcagacg ggctaaggt caattgtca tggtctgtg ctggtacag gcatgatgt tttatatgt cggcagaac tggcacttg ttggcttgg ctattgtgc ccccggttg cgattattg ccgttattt atgggagat tccttagag ggatttcta ggaaggttc aggattctt tagcagggg ctactagtg ttgcagagg aacgtattg ctaatatca ggactgtta aaactttct cgcaggaac cgagagaag ttgattcgt acgcaaatg ccattaaag aggttctag gcttgagtt acagagagg ctaaagcta gggccattt tttatgggc tgaccggct ttagcggcc acgtaatca taatatcag tgctttatt atggtggtg tgatggtgt cttcaaaca ccataacag tgggaaatc tgtcttctt ttctcttat atgcggctt atatcggcg tatcgttgg gcacatttt caaattttt actccgaat tgaacaaat caattggtg ctgcggcca gaatttggg aaattatcg acagagaac ccataattc cgatgaaag gtggtttag tgccaattg caccccctg aaggtcaca tacaattta acgacatcc agtttagtt acccagcgc gcgacgatg tccgcattt tcgaaaatt tcaaactcg aaatatccc ccggaaaaa tggttgcag tagtcggtc cgagtggtt ccggcaaaa gcaccctcg ctgctcttt tattgcgac tatacgatc ctgcaaaag gacaagttc tattagacg gacaggaca ttaaagaaa ttgatccga gttggttgc gccgacatg tcggtaccg ttagccagg aaccagttt tattttcgt gttcaattc gtgataata ttttatacg gagctgatg atccttcca aagtgacag aagatgaat tagttcaag tggccaaag aggccaatg tttacgaat tcgtccaac agttgcccg acggttttg atactttgg taggggaac gtggggtga tgttgagcg gtggccaga aacaacgca ttgccatag cgagagctt tgattaaag atccgaaga tattgttgt tagatgaag ccacgagtg cgttagatg cgcaatcgg agcacttgg tgcaagaag ctttggaga ggattatga aagggagga ctgttttaa cgattgctc ataggctgt cgacgattc aaaatgccg atactattg ctgtgctgc gcgatggac atatcgtgg aacagggga aataccagg atttactta atatcaaga agggcgcgt ttagggaat taatgaaac atcaaacgt ttcaaggga atatagaga agacgtgat gtacttaat ttattttat ttttacact tttgaggac ttgttacac gtttatttg ttatgcaaa taaaatagt tttactgtt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
