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Gene information
General information
TC007080 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9986349..9992254
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007080
    iBeetle screen iB_08175

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007080 RA
      MMRILLWST LVLVHSHPA QKLNLPELD VSTPEEVNS 
      LEREEPLSR SIDPQNSRN KLDDTVQVV EEILKADPT 
      LPRLTRGEI LDLLENITS SDDQKKFLR GNRDPKAVM 
      VVMPYTPTN DKNMEDLYT KAPVTHIIG AEPFKTKEE 
      ADKVKHSRR RPPVQFNDT DNDLAKKPL HLEEKKKYS 
      ASISEKVTK PTTIPRNQV RRRRPEATT EAPKPTPVT 
      TNQPKSRRP VRRRTTTTP TTYHPNHRY PEDFLPSQG 
      IRIIESPKL SQKNDVSLP DLDFEENVK HEVSELPPK 
      PLTEEIMVP DHLKTVMKD LNLESALKP QENLVEHNR 
      IKNILASIG VLPDGTTTT TTTEAPNAE MVAESLSPE 
      MRDLLMSFG LLPNPNPKP TQKPTRDDQ YFPDKAEVK 
      PESYVGFKP LPDDGPSRD DMDALLASF GLGRSARRG 
      KVLKAESRS DKPDEKYNF DMIPEHLKG VISDLGISQ 
      ERDGRKIRT SSEKQHVFN PDNQYATEE ELKKLERLI 
      GMIKQLEKM NGTATEEDL KKFDLEGLK ELVSSLNGD 
      NGTQTLDQK ELDSPNPNN FDVGLVKNE IKRQESTTT 
      TTSKPEAET PSLTALEES FGGQTETVT EPPVPETTT 
      RKTGFYYLV DWNTFLDID DTKGKRVNL RFQPTIGDP 
      KRFLSVSVP
    CDS sequence
    • >TC007080 RA
      atgatgcga attttgctt tggtcaacg ctcgtcctg 
      gtacactca cacccagcc caaaaactc aacttacca 
      gagctggac gtttcaaca ccggaagaa gtcaacagt 
      ctagaacgc gaggaacca ctcagtcgc tccattgac 
      ccacaaaac agccgcaat aagctcgat gatacagtg 
      caagtggtc gaggaaata ctcaaagcc gaccccact 
      ttacctcgc ttgacccgt ggcgaaatt ttagaccta 
      ctcgaaaat atcacaagt tcggatgac caaaaaaag 
      tttttgcgc gggaatcga gacccgaaa gctgtgatg 
      gtcgtaatg ccctataca cccaccaat gacaaaaac 
      atggaagat ctgtatacc aaagcaccc gtgactcac 
      ataatcgga gctgagcct tttaaaacc aaggaggag 
      gcggacaaa gtgaagcat tcgaggaga cgaccaccg 
      gttcaattc aacgatact gataacgat ttggcgaag 
      aaaccgctg catttggaa gaaaagaag aaatattcg 
      gcgagtatc agtgagaag gttactaaa ccaacaacc 
      attccgcga aaccaagtg cgaagaaga cgcccagag 
      gccacaact gaagcccca aaaccaaca ccggtgacc 
      acaaaccag cccaagtcg cggcgccct gtcaggagg 
      cgaaccacc acgacccca accacttac caccccaac 
      catcgctac cccgaagat ttcctcccc agtcaagga 
      atccgcata attgaatca ccgaaactg tcccagaaa 
      aacgatgtt tctctcccc gatctcgat ttcgaagaa 
      aacgtcaaa cacgaagtt tcggaactt ccgccaaaa 
      ccactcact gaagaaatc atggtcccg gatcacttg 
      aaaacagtt atgaaagac ttgaatctg gaatcagcc 
      ttaaaaccg caagaaaat ctagtggaa cacaaccgg 
      atcaagaac attttagcc tcaattggg gttctccca 
      gatggtacc accaccaca accacgaca gaggcaccc 
      aatgccgaa atggtcgcc gagtcttta agcccagaa 
      atgcgcgat cttttgatg agtttcggg ttattaccg 
      aacccgaac ccgaaaccg acccaaaaa ccgacaagg 
      gatgatcag tacttcccc gacaaggct gaagtcaag 
      ccggaatcg tacgtcggg ttcaaacca ttgcctgac 
      gatgggccg agtcgtgac gacatggac gcccttcta 
      gcctccttc ggcttgggg cgaagcgcc cgaagaggc 
      aaagtcctc aaagctgaa agtcgcagc gacaaaccg 
      gacgaaaag tataatttt gacatgatt cccgaacac 
      ttgaaaggc gttatcagc gatttgggg atttcgcag 
      gaacgcgac gggcgaaaa attcgaacc tcgtcggag 
      aaacaacac gtcttcaac cccgataac cagtacgcc 
      actgaggaa gaattgaag aagcttgag cgtctcatt 
      ggtatgatc aaacagttg gagaagatg aacgggact 
      gcgactgaa gaagacttg aagaaattc gacttggaa 
      ggcttgaag gaacttgtc agtagcctc aacggggat 
      aatggcact caaactttg gaccaaaaa gagttagac 
      tcgccaaac ccgaacaat tttgacgtg gggcttgtc 
      aagaatgaa atcaaaaga caggaaagt acgactaca 
      actacgagt aaaccggag gcggaaact ccgtccctt 
      acagctttg gaggagtct ttcggtggt caaaccgaa 
      actgtgacc gaaccaccg gttccagaa accacaact 
      cgcaaaact gggttctac tatttggtg gattggaac 
      acgtttttg gacattgac gacacgaaa gggaaaagg 
      gttaatttg aggttccag cctacgatt ggtgacccg 
      aagaggttc ttgtcagtt agtgtccct tga
    mRNA sequence
    • >TC007080 RA
      accctaaat atagacttt ttattggtc agatagatt 
      gtgactgtg aaagtcttt agacgttca agttctctc 
      atattatgc aataaataa atcaaacag tgcttggtg 
      agatgatcg tgatcttca tttgaattc gcgtagaaa 
      gtacaagaa agttggacg gaatattat acgtggaaa 
      atgatgcga attttgctt tggtcaacg ctcgtcctg 
      gtacactca cacccagcc caaaaactc aacttacca 
      gagctggac gtttcaaca ccggaagaa gtcaacagt 
      ctagaacgc gaggaacca ctcagtcgc tccattgac 
      ccacaaaac agccgcaat aagctcgat gatacagtg 
      caagtggtc gaggaaata ctcaaagcc gaccccact 
      ttacctcgc ttgacccgt ggcgaaatt ttagaccta 
      ctcgaaaat atcacaagt tcggatgac caaaaaaag 
      tttttgcgc gggaatcga gacccgaaa gctgtgatg 
      gtcgtaatg ccctataca cccaccaat gacaaaaac 
      atggaagat ctgtatacc aaagcaccc gtgactcac 
      ataatcgga gctgagcct tttaaaacc aaggaggag 
      gcggacaaa gtgaagcat tcgaggaga cgaccaccg 
      gttcaattc aacgatact gataacgat ttggcgaag 
      aaaccgctg catttggaa gaaaagaag aaatattcg 
      gcgagtatc agtgagaag gttactaaa ccaacaacc 
      attccgcga aaccaagtg cgaagaaga cgcccagag 
      gccacaact gaagcccca aaaccaaca ccggtgacc 
      acaaaccag cccaagtcg cggcgccct gtcaggagg 
      cgaaccacc acgacccca accacttac caccccaac 
      catcgctac cccgaagat ttcctcccc agtcaagga 
      atccgcata attgaatca ccgaaactg tcccagaaa 
      aacgatgtt tctctcccc gatctcgat ttcgaagaa 
      aacgtcaaa cacgaagtt tcggaactt ccgccaaaa 
      ccactcact gaagaaatc atggtcccg gatcacttg 
      aaaacagtt atgaaagac ttgaatctg gaatcagcc 
      ttaaaaccg caagaaaat ctagtggaa cacaaccgg 
      atcaagaac attttagcc tcaattggg gttctccca 
      gatggtacc accaccaca accacgaca gaggcaccc 
      aatgccgaa atggtcgcc gagtcttta agcccagaa 
      atgcgcgat cttttgatg agtttcggg ttattaccg 
      aacccgaac ccgaaaccg acccaaaaa ccgacaagg 
      gatgatcag tacttcccc gacaaggct gaagtcaag 
      ccggaatcg tacgtcggg ttcaaacca ttgcctgac 
      gatgggccg agtcgtgac gacatggac gcccttcta 
      gcctccttc ggcttgggg cgaagcgcc cgaagaggc 
      aaagtcctc aaagctgaa agtcgcagc gacaaaccg 
      gacgaaaag tataatttt gacatgatt cccgaacac 
      ttgaaaggc gttatcagc gatttgggg atttcgcag 
      gaacgcgac gggcgaaaa attcgaacc tcgtcggag 
      aaacaacac gtcttcaac cccgataac cagtacgcc 
      actgaggaa gaattgaag aagcttgag cgtctcatt 
      ggtatgatc aaacagttg gagaagatg aacgggact 
      gcgactgaa gaagacttg aagaaattc gacttggaa 
      ggcttgaag gaacttgtc agtagcctc aacggggat 
      aatggcact caaactttg gaccaaaaa gagttagac 
      tcgccaaac ccgaacaat tttgacgtg gggcttgtc 
      aagaatgaa atcaaaaga caggaaagt acgactaca 
      actacgagt aaaccggag gcggaaact ccgtccctt 
      acagctttg gaggagtct ttcggtggt caaaccgaa 
      actgtgacc gaaccaccg gttccagaa accacaact 
      cgcaaaact gggttctac tatttggtg gattggaac 
      acgtttttg gacattgac gacacgaaa gggaaaagg 
      gttaatttg aggttccag cctacgatt ggtgacccg 
      aagaggttc ttgtcagtt agtgtccct tgaactctt 
      gctcaatta tttattaat tttaagaat gtacatagt 
      agcaaacac gtttggtgt ggaaagtgt tcttaacgt 
      actccagtt atttacaaa gtagtagta agtgaaaat 
      atgtaatta aaactggaa gtttttgta aacaatatt 
      taattccac ttcgctgtc tctgtggta tgatcaatt 
      aaatagttg atttattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY