Status
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Gene information
General information
TC007057 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10350082..10352075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007057
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08174

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007057 RA
      MNSVFQLTD CCRTCLRVE SALTPLDSV DSDNIKLCD 
      KLVACISDI AWTKNGYSN LLCSNCIEK LRSAYEFRL 
      LCIQSENTY QQYFSQEKV ASYTNLDDF QLTNTIQIQ 
      GAVIETVKT NDFLNLKHF LDDDTGNKP PENSRSASP 
      DSVATTGFA KYEKIVKKA VVPTRSVKT QTQPLPQYP 
      SLTPAAARL EAQQSKTTI IIEDPYKFT CELCGKKYA 
      KNANLKIHM RTHTGEKPF ECKYCDKKF YHSSHLREH 
      IRRHTGEKP FQCNVCQKR FTITAELTV HMRIHTGEK 
      PYACNCCEK RYIKASDLQ VHMRSHTGE KPFACNYCD 
      KKFTSGYIL NSHLKTHTG DRPWQCNIC SKSFTQSSH 
      LTVHMKKHT GEKFVCKVC NEEFTHSSQ LTVHMRQHT 
      GRQPYKCTM CDKICNYAS ELQTHIMKH TGEKFTCIT 
      CDKKFTTAA YLQEHTRTH TGENLFNCT LCERVFTRA 
      TYLDKHLRT HTGERPFEC EICGKKFTQ SSSLNVHIR 
      KHTGEKPYT CNICNRSFA TSSYLSVHL RKHKKYINM
    CDS sequence
    • >TC007057 RA
      atgaacagt gtgttccag ctaacggac tgctgtcgc 
      acttgtttg cgcgttgaa agtgcttta accccgttg 
      gattcggtc gactccgac aatataaaa ttatgtgat 
      aaacttgtt gcttgcatt tcggatatt gcttggacg 
      aaaaatgga tactccaac ttgttgtgt tcgaattgt 
      atcgaaaaa ctccggagt gcgtacgag ttccgatta 
      ttgtgtatc caatcggag aacacttat cagcagtat 
      ttttcccag gaaaaagtc gcctcttac acaaatttg 
      gacgacttt cagctgact aacacaatt caaatccaa 
      ggtgctgtc atcgaaact gttaaaacc aatgatttc 
      ctaaacctg aagcatttc cttgacgat gacacagga 
      aataagccg ccggagaat tcgcgaagt gcgtcccca 
      gattcagtt gccactact ggttttgca aaatatgaa 
      aaaatcgtg aaaaaagcg gtggtgcca acccggtct 
      gtgaaaacc caaacgcag cctttgccc caatatcca 
      agtctgact ccggcggca gcacggctg gaggcccaa 
      caaagcaaa accacgatt atcatagaa gacccctac 
      aagttcacg tgcgagctt tgcgggaaa aaatacgcc 
      aaaaacgcc aatctcaaa attcacatg aggactcac 
      accggggaa aaaccgttc gagtgcaaa tactgcgac 
      aagaagttc tatcactcg tcgcatttg cgggaacac 
      atcaggcga cacacgggg gaaaaaccg ttccagtgc 
      aacgtgtgt caaaaacgc ttcacgatc actgcagag 
      ctgacagtc cacatgcga atccacacc ggggaaaaa 
      ccgtacgcg tgtaattgc tgcgaaaag cgctacatc 
      aaagccagc gatctgcag gtgcacatg cggtcgcac 
      accggggaa aaacccttc gcatgtaat tattgcgat 
      aaaaaattc acttcgggt tatatactg aattcgcac 
      ctgaaaacg cataccgga gaccggccc tggcagtgc 
      aacatttgc tcaaagtcg ttcactcaa tcgtcgcat 
      ttaacagtc catatgaag aaacacact ggggagaag 
      ttcgtttgt aaagtgtgc aatgaagag ttcacccat 
      tcgtcccaa ttgacggtg catatgagg caacacaca 
      ggccgccaa ccttacaaa tgcacaatg tgcgataaa 
      atttgcaat tacgcgtct gagttgcaa acgcacatt 
      atgaaacac actggggaa aaattcacg tgcataacg 
      tgcgataaa aaattcaca acggcggcg tatttgcag 
      gaacatacg cggacgcac actggcgaa aatttgttc 
      aattgtacg ttgtgtgaa cgcgttttt acgagagca 
      acctatctt gataaacat ttaaggacg catactggc 
      gaaaggcct ttcgagtgt gaaatttgc gggaaaaaa 
      ttcacacag agtagtagt ctcaatgta catatcagg 
      aaacataca ggggaaaaa ccctacact tgtaatatc 
      tgtaatagg agttttgca acgtcgtct tacctatcc 
      gttcattta aggaagcat aaaaaatat attaacatg 
      taa
    mRNA sequence
    • >TC007057 RA
      gtttataaa taaacaaca atttactgg tgcttgagg 
      caccctaat gttaatttt gaaaaatga acagtgtgt 
      tccagctaa cggactgct gtcgcactt gtttgcgcg 
      ttgaaagtg ctttaaccc cgttggatt cggtcgact 
      ccgacaata taaaattat gtgataaac ttgttgctt 
      gcatttcgg atattgctt ggacgaaaa atggatact 
      ccaacttgt tgtgttcga attgtatcg aaaaactcc 
      ggagtgcgt acgagttcc gattattgt gtatccaat 
      cggagaaca cttatcagc agtattttt cccaggaaa 
      aagtcgcct cttacacaa atttggacg actttcagc 
      tgactaaca caattcaaa tccaaggtg ctgtcatcg 
      aaactgtta aaaccaatg atttcctaa acctgaagc 
      atttccttg acgatgaca caggaaata agccgccgg 
      agaattcgc gaagtgcgt ccccagatt cagttgcca 
      ctactggtt ttgcaaaat atgaaaaaa tcgtgaaaa 
      aagcggtgg tgccaaccc ggtctgtga aaacccaaa 
      cgcagcctt tgccccaat atccaagtc tgactccgg 
      cggcagcac ggctggagg cccaacaaa gcaaaacca 
      cgattatca tagaagacc cctacaagt tcacgtgcg 
      agctttgcg ggaaaaaat acgccaaaa acgccaatc 
      tcaaaattc acatgagga ctcacaccg gggaaaaac 
      cgttcgagt gcaaatact gcgacaaga agttctatc 
      actcgtcgc atttgcggg aacacatca ggcgacaca 
      cgggggaaa aaccgttcc agtgcaacg tgtgtcaaa 
      aacgcttca cgatcactg cagagctga cagtccaca 
      tgcgaatcc acaccgggg aaaaaccgt acgcgtgta 
      attgctgcg aaaagcgct acatcaaag ccagcgatc 
      tgcaggtgc acatgcggt cgcacaccg gggaaaaac 
      ccttcgcat gtaattatt gcgataaaa aattcactt 
      cgggttata tactgaatt cgcacctga aaacgcata 
      ccggagacc ggccctggc agtgcaaca tttgctcaa 
      agtcgttca ctcaatcgt cgcatttaa cagtccata 
      tgaagaaac acactgggg agaagttcg tttgtaaag 
      tgtgcaatg aagagttca cccattcgt cccaattga 
      cggtgcata tgaggcaac acacaggcc gccaacctt 
      acaaatgca caatgtgcg ataaaattt gcaattacg 
      cgtctgagt tgcaaacgc acattatga aacacactg 
      gggaaaaat tcacgtgca taacgtgcg ataaaaaat 
      tcacaacgg cggcgtatt tgcaggaac atacgcgga 
      cgcacactg gcgaaaatt tgttcaatt gtacgttgt 
      gtgaacgcg tttttacga gagcaacct atcttgata 
      aacatttaa ggacgcata ctggcgaaa ggcctttcg 
      agtgtgaaa tttgcggga aaaaattca cacagagta 
      gtagtctca atgtacata tcaggaaac atacagggg 
      aaaaaccct acacttgta atatctgta ataggagtt 
      ttgcaacgt cgtcttacc tatccgttc atttaagga 
      agcataaaa aatatatta acatgtaat tatctcacg 
      gaggactaa tgcattagg aggcgacag cgcacaaat 
      cgaacagta attttatcg aaagcaata caatgtaag 
      tctgctaaa agtatacaa gaactctta atttatatg 
      cataaactt tgggccaac aaatggtga aaaaaaatt 
      ctggtaaaa gttttttaa ttaccgaga taaaaacac 
      cactaaact tttttggga acaatcgac attttattt 
      attgttttt cgtttagta taataaata taaaaaaat 
      ata
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