General information | |||
TC008448 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 10472643..10526472 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC008448 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08172 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
larva dorsal bent - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment anterior number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC008448 RA
MSWRGALVF LILSLTQCS GCPVFCSCK WKSGKQTVE CINKDLLVI PEGMDSSTQ VLQFCGNNL QTLQRDKFL KMDLINLQR IYLCRCRIT SIDDRTFRG LTNLVELDL SGNLLETVP SETFLDCPS LMRLSLNAN PIKTLRRAA FNHLSFLNT IELSNCEIS NVEQGAFQG LYSLEWLHL NGNKMTTLQ GATYLPKSL KGVQLQENP WECDCHILE LHAWLRAFT MPHSVEPLC NGPTRLRSR TIKSVPVGE LACLPEVSP TMFYLEIGE GKNVTLLCQ VNAIPEARI SWTFQGQLL QNDSMIAPG VHLLYFIEE GAVEKRSEL FIYNSNSDD NGTFICNAE NAAGLVQAN FTIRVIVKH DTPPVTNEL PFEFILITL SAAAVSILL LLVVILLSI IKCHRNARL KKKRNNSKA ALSNTTKDN LLQESVDDY SEKPPTQEE ETMLYNTPP EDLPVSSIP PTLTPYQLE QNPDLINGT ESVGRFTPL KKRDLYVYA PDVHLNPVG LLNSGSNCY KTLPYNRNK RSNAANPAG RFSREAEFL QRTMHPSYE HYSDVRYTA DGYPVRTNE GAVEPCCST TTTVSWPSC VPASFVAKD CVKSVGAQT EDTNTKCEP IKETFTESL DEGYEGEGA EVHTNGEAR YLPSCGSLN KLILPSPTC VLPYEYKFN R
- >TC008448 RA
atgtcttgg agaggtgca cttgtgttt ctcatattg tctctgact cagtgtagt ggttgtccg gttttctgt tcgtgcaag tggaagagc ggtaagcag acagtcgag tgcatcaat aaggacctc cttgtcatc cccgaaggt atggattcc agtactcaa gtgctgcag ttttgcggg aacaatttg caaacactg cagcgcgac aagttcctc aaaatggac ttaattaac ttacaacgg atatatttg tgccgttgc cgcattaca agcatcgac gatcggacg ttccgcggg ttaacaaac ctcgtcgaa ttggatttg tccggcaac ttgctcgag acggttccc agtgaaacg ttcctcgac tgcccttcg ctaatgagg ctctcactt aatgctaat cctataaag accctccga cgggcggca ttcaaccat ctgtctttc ctaaacacc atcgagctc agcaattgc gagatatca aacgtggag cagggggcc ttccagggg ctgtacagt ctcgagtgg ctgcacctc aacggaaac aagatgacg acgctgcaa ggagcgacc tatctgccg aaatctttg aaaggggtg caactccag gaaaatcca tgggagtgc gactgccac atacttgaa cttcacgct tggctgagg gctttcacc atgccacat tccgtcgag ccgctctgt aatggacca actcgactc cggagtcgc acaattaaa tcggtgccg gtgggagaa ctcgcttgt ttacctgaa gtttcaccc acgatgttc tatctcgaa atcggggaa ggcaaaaat gtcacgctt ttatgccaa gttaacgca atccccgaa gcgagaatt tcctggacg ttccaagga caattgcta cagaacgac tccatgatt gcaccaggt gttcatctc ttgtatttc atcgaggag ggagccgtc gaaaaacgc agcgaactc ttcatttac aattctaac agtgacgac aacggaacc ttcatatgc aacgccgaa aatgcagcc ggacttgtc caagcaaat ttcacgatt cgagtaatc gtcaaacac gacactcca ccagtcaca aacgaactc cccttcgag ttcatccta atcacgctc tcagcagcg gctgtctca atcctcctc ttgttagtc gtaatactc ctatctata ataaaatgc caccgaaac gcccgattg aaaaagaaa cgaaacaac tcgaaagcc gctctcagt aacacaacc aaggacaat cttctccaa gaaagcgtc gacgactac tcggagaaa cccccaact caggaggaa gaaaccatg ctttacaac acccctcct gaagacctc cccgtgtct tcaatcccc ccgacactc acgccgtac cagctagag caaaacccc gacctcatc aacggcact gaaagtgtg gggcggttc acccccttg aaaaaacgc gacctgtac gtctacgcc cccgacgtt cacctcaac cccgtgggg ttgctcaac tcgggctcg aactgctac aaaacgctc ccctacaac aggaacaag cgctccaat gctgccaat ccagctggg aggttctcc cgcgaagct gagttcctc caacggacg atgcatccg tcgtatgag cactacagt gacgtgagg tacactgca gacgggtat ccggtgagg accaacgag ggggctgtg gagccctgc tgctccaca acgactacg gttagttgg ccgtcgtgt gtccccgcg agttttgtt gcgaaagac tgtgtgaag agtgtgggt gctcagact gaggacaca aacaccaaa tgtgagccg attaaagag acgttcacg gagagtctg gatgaggga tacgaaggg gaaggggcg gaggtacat actaatggg gaggcgcgc tatttgccc tcctgtggt tcactaaac aaattaata ttaccatct cccacatgt gtgcttccc tacgagtac aaatttaac agataa
- >TC008448 RA
aagcgctgc gcttaatac catatcgcg tcgaatctg tgcaaaaat ccaccaatt ttaaactag caacatcac caatttgga atttttgtg attcgggtc gcaaaccgc caaaatgtc tcgctggtc gatgattgc gctggtttg ttgaccgtc gatgagatg cagaatgtc ttggagagg tgcacttgt gtttctcat attgtctct gactcagtg tagtggttg tccggtttt ctgttcgtg caagtggaa gagcggtaa gcagacagt cgagtgcat caataagga cctccttgt catccccga aggtatgga ttccagtac tcaagtgct gcagttttg cgggaacaa tttgcaaac actgcagcg cgacaagtt cctcaaaat ggacttaat taacttaca acggatata tttgtgccg ttgccgcat tacaagcat cgacgatcg gacgttccg cgggttaac aaacctcgt cgaattgga tttgtccgg caacttgct cgagacggt tcccagtga aacgttcct cgactgccc ttcgctaat gaggctctc acttaatgc taatcctat aaagaccct ccgacgggc ggcattcaa ccatctgtc tttcctaaa caccatcga gctcagcaa ttgcgagat atcaaacgt ggagcaggg ggccttcca ggggctgta cagtctcga gtggctgca cctcaacgg aaacaagat gacgacgct gcaaggagc gacctatct gccgaaatc tttgaaagg ggtgcaact ccaggaaaa tccatggga gtgcgactg ccacatact tgaacttca cgcttggct gagggcttt caccatgcc acattccgt cgagccgct ctgtaatgg accaactcg actccggag tcgcacaat taaatcggt gccggtggg agaactcgc ttgtttacc tgaagtttc acccacgat gttctatct cgaaatcgg ggaaggcaa aaatgtcac gcttttatg ccaagttaa cgcaatccc cgaagcgag aatttcctg gacgttcca aggacaatt gctacagaa cgactccat gattgcacc aggtgttca tctcttgta tttcatcga ggagggagc cgtcgaaaa acgcagcga actcttcat ttacaattc taacagtga cgacaacgg aaccttcat atgcaacgc cgaaaatgc agccggact tgtccaagc aaatttcac gattcgagt aatcgtcaa acacgacac tccaccagt cacaaacga actcccctt cgagttcat cctaatcac gctctcagc agcggctgt ctcaatcct cctcttgtt agtcgtaat actcctatc tataataaa atgccaccg aaacgcccg attgaaaaa gaaacgaaa caactcgaa agccgctct cagtaacac aaccaagga caatcttct ccaagaaag cgtcgacga ctactcgga gaaaccccc aactcagga ggaagaaac catgcttta caacacccc tcctgaaga cctccccgt gtcttcaat ccccccgac actcacgcc gtaccagct agagcaaaa ccccgacct catcaacgg cactgaaag tgtggggcg gttcacccc cttgaaaaa acgcgacct gtacgtcta cgcccccga cgttcacct caaccccgt ggggttgct caactcggg ctcgaactg ctacaaaac gctccccta caacaggaa caagcgctc caatgctgc caatccagc tgggaggtt ctcccgcga agctgagtt cctccaacg gacgatgca tccgtcgta tgagcacta cagtgacgt gaggtacac tgcagacgg gtatccggt gaggaccaa cgagggggc tgtggagcc ctgctgctc cacaacgac tacggttag ttggccgtc gtgtgtccc cgcgagttt tgttgcgaa agactgtgt gaagagtgt gggtgctca gactgagga cacaaacac caaatgtga gccgattaa agagacgtt cacggagag tctggatga gggatacga aggggaagg ggcggaggt acatactaa tggggaggc gcgctattt gccctcctg tggttcact aaacaaatt aatattacc atctcccac atgtgtgct tccctacga gtacaaatt taacagata aacagtcta tatcgtcac ggagtaggc tgatactat gaattatgc gccaacaag ctgatttat cagagtcga gctcaaaag ttttgcccc aagattcac aaagttatg gaataagaa aataaatac aggtgatgc gatacaatc gagaagttt tctaattac ataagtcgg cggacaaca aattgaata aaactcgaa tgataaatt atttattgc catttcgat gagctccaa atcgaatga gccttccca ttcaagggc cttttcaat cacgagata aggtatttg cctatttaa agtttcaaa actgtccgg acgaatgaa aaggtattc acaagctcc ctcctgatg gtttaattt ttcaccgct ctaattttc tgctcattt actcaaaca tgcgatgaa atttattgt ttagtaagt tttattgac ttgaatgga gattttaat attcaaact tttgtcgca taaagtgtt tcaacgatg cgagaagtg gaagataaa gacttgaag atattggaa cgcgatttt tatggagtc atagaggtt cagtaatgc tcgtttaaa ctagggaca tgctacttg aaatggatt tagtttaaa tcatgacaa gaaagataa atggaaagc ttttaattt aatatgaag ggtcattgc tgaaatagc caataaaaa ttttacagg acgcataaa aacgcggct ttatttcta ttcctaaaa taaaaaccg ccacttccg tggcaagaa ttttaacta attggtgat cattttatt caattttgt tgttgtaga cgaacttta ataaagtaa aaagctgtt gcaaactat tctgcacta aattccgcc gagatattg ccatgttaa caattttgg cgtctgata ttcgtctct caagacagt gactaaagt cagatgggt actaaacta acgctcatt gacaataat gtccgttac aattacgct gacttgctc catctagca ttaactcta atccattgt aattcatgc ccgacactg ttaattacc aacgtacat tagatcagt acttgtcta ttaagaatc aacgaattc aattaaacc ggattttta tcacaattt ctgcgccaa caggttgtg tacgagtat ttaattaaa atcgggcaa cggtcattt taaaagaat gatgagtgt cgagcttaa cgcggcaac gtttggccg aacaaaatg aatataagg tacatgctg aaagaaatt taattaaca aaagctttg aatataaag gatttatgg ttattaaat cagtgtttg aaaaatcat gtctgtagc agctgaatc aacaaatgg aattgagtc ggtagaacg acctggatg atttctatg attaacttt tgttgcgac tactgtttg gaaatggtt ttaaagaga taacaaatc acatttaat gcaaatatg tttgattgt ggtgtggct ttgcaatta atgtgacac caacaatta aataagcca tgtccacag agatcacta aacgcggaa ttagaccag attaattaa ctaattaaa cgaggaaaa acgcaactc acccaaaat aataaatcg ttaaagcat gaattacct ggaacaact tttttattt ttagttaat ttgttacat tttttctaa attcaggaa acttagttg aaagcagat gatcacaga catcacagt ttagtgacc catgttagt tcagagaca aaatgtcgt gtcagttgc tctattcgt tacaattta gctaaagat agttacgag aggaataag aaaagaaca ccaagcaca aaagactga aatgctgat tgagtgttg atactatga tagaaaaat taaattttc acgataagc taagagcat tgacaacaa taatatttt tactgaact aatagggac ttatttttt cttaaaagc attaaaaaa atcttagat ct
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
