Status
Please wait ...
Gene information
General information
TC008448 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10472643..10526472
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008448
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08172

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment anterior number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008448 RA
      MSWRGALVF LILSLTQCS GCPVFCSCK WKSGKQTVE 
      CINKDLLVI PEGMDSSTQ VLQFCGNNL QTLQRDKFL 
      KMDLINLQR IYLCRCRIT SIDDRTFRG LTNLVELDL 
      SGNLLETVP SETFLDCPS LMRLSLNAN PIKTLRRAA 
      FNHLSFLNT IELSNCEIS NVEQGAFQG LYSLEWLHL 
      NGNKMTTLQ GATYLPKSL KGVQLQENP WECDCHILE 
      LHAWLRAFT MPHSVEPLC NGPTRLRSR TIKSVPVGE 
      LACLPEVSP TMFYLEIGE GKNVTLLCQ VNAIPEARI 
      SWTFQGQLL QNDSMIAPG VHLLYFIEE GAVEKRSEL 
      FIYNSNSDD NGTFICNAE NAAGLVQAN FTIRVIVKH 
      DTPPVTNEL PFEFILITL SAAAVSILL LLVVILLSI 
      IKCHRNARL KKKRNNSKA ALSNTTKDN LLQESVDDY 
      SEKPPTQEE ETMLYNTPP EDLPVSSIP PTLTPYQLE 
      QNPDLINGT ESVGRFTPL KKRDLYVYA PDVHLNPVG 
      LLNSGSNCY KTLPYNRNK RSNAANPAG RFSREAEFL 
      QRTMHPSYE HYSDVRYTA DGYPVRTNE GAVEPCCST 
      TTTVSWPSC VPASFVAKD CVKSVGAQT EDTNTKCEP 
      IKETFTESL DEGYEGEGA EVHTNGEAR YLPSCGSLN 
      KLILPSPTC VLPYEYKFN R
    CDS sequence
    • >TC008448 RA
      atgtcttgg agaggtgca cttgtgttt ctcatattg 
      tctctgact cagtgtagt ggttgtccg gttttctgt 
      tcgtgcaag tggaagagc ggtaagcag acagtcgag 
      tgcatcaat aaggacctc cttgtcatc cccgaaggt 
      atggattcc agtactcaa gtgctgcag ttttgcggg 
      aacaatttg caaacactg cagcgcgac aagttcctc 
      aaaatggac ttaattaac ttacaacgg atatatttg 
      tgccgttgc cgcattaca agcatcgac gatcggacg 
      ttccgcggg ttaacaaac ctcgtcgaa ttggatttg 
      tccggcaac ttgctcgag acggttccc agtgaaacg 
      ttcctcgac tgcccttcg ctaatgagg ctctcactt 
      aatgctaat cctataaag accctccga cgggcggca 
      ttcaaccat ctgtctttc ctaaacacc atcgagctc 
      agcaattgc gagatatca aacgtggag cagggggcc 
      ttccagggg ctgtacagt ctcgagtgg ctgcacctc 
      aacggaaac aagatgacg acgctgcaa ggagcgacc 
      tatctgccg aaatctttg aaaggggtg caactccag 
      gaaaatcca tgggagtgc gactgccac atacttgaa 
      cttcacgct tggctgagg gctttcacc atgccacat 
      tccgtcgag ccgctctgt aatggacca actcgactc 
      cggagtcgc acaattaaa tcggtgccg gtgggagaa 
      ctcgcttgt ttacctgaa gtttcaccc acgatgttc 
      tatctcgaa atcggggaa ggcaaaaat gtcacgctt 
      ttatgccaa gttaacgca atccccgaa gcgagaatt 
      tcctggacg ttccaagga caattgcta cagaacgac 
      tccatgatt gcaccaggt gttcatctc ttgtatttc 
      atcgaggag ggagccgtc gaaaaacgc agcgaactc 
      ttcatttac aattctaac agtgacgac aacggaacc 
      ttcatatgc aacgccgaa aatgcagcc ggacttgtc 
      caagcaaat ttcacgatt cgagtaatc gtcaaacac 
      gacactcca ccagtcaca aacgaactc cccttcgag 
      ttcatccta atcacgctc tcagcagcg gctgtctca 
      atcctcctc ttgttagtc gtaatactc ctatctata 
      ataaaatgc caccgaaac gcccgattg aaaaagaaa 
      cgaaacaac tcgaaagcc gctctcagt aacacaacc 
      aaggacaat cttctccaa gaaagcgtc gacgactac 
      tcggagaaa cccccaact caggaggaa gaaaccatg 
      ctttacaac acccctcct gaagacctc cccgtgtct 
      tcaatcccc ccgacactc acgccgtac cagctagag 
      caaaacccc gacctcatc aacggcact gaaagtgtg 
      gggcggttc acccccttg aaaaaacgc gacctgtac 
      gtctacgcc cccgacgtt cacctcaac cccgtgggg 
      ttgctcaac tcgggctcg aactgctac aaaacgctc 
      ccctacaac aggaacaag cgctccaat gctgccaat 
      ccagctggg aggttctcc cgcgaagct gagttcctc 
      caacggacg atgcatccg tcgtatgag cactacagt 
      gacgtgagg tacactgca gacgggtat ccggtgagg 
      accaacgag ggggctgtg gagccctgc tgctccaca 
      acgactacg gttagttgg ccgtcgtgt gtccccgcg 
      agttttgtt gcgaaagac tgtgtgaag agtgtgggt 
      gctcagact gaggacaca aacaccaaa tgtgagccg 
      attaaagag acgttcacg gagagtctg gatgaggga 
      tacgaaggg gaaggggcg gaggtacat actaatggg 
      gaggcgcgc tatttgccc tcctgtggt tcactaaac 
      aaattaata ttaccatct cccacatgt gtgcttccc 
      tacgagtac aaatttaac agataa
    mRNA sequence
    • >TC008448 RA
      aagcgctgc gcttaatac catatcgcg tcgaatctg 
      tgcaaaaat ccaccaatt ttaaactag caacatcac 
      caatttgga atttttgtg attcgggtc gcaaaccgc 
      caaaatgtc tcgctggtc gatgattgc gctggtttg 
      ttgaccgtc gatgagatg cagaatgtc ttggagagg 
      tgcacttgt gtttctcat attgtctct gactcagtg 
      tagtggttg tccggtttt ctgttcgtg caagtggaa 
      gagcggtaa gcagacagt cgagtgcat caataagga 
      cctccttgt catccccga aggtatgga ttccagtac 
      tcaagtgct gcagttttg cgggaacaa tttgcaaac 
      actgcagcg cgacaagtt cctcaaaat ggacttaat 
      taacttaca acggatata tttgtgccg ttgccgcat 
      tacaagcat cgacgatcg gacgttccg cgggttaac 
      aaacctcgt cgaattgga tttgtccgg caacttgct 
      cgagacggt tcccagtga aacgttcct cgactgccc 
      ttcgctaat gaggctctc acttaatgc taatcctat 
      aaagaccct ccgacgggc ggcattcaa ccatctgtc 
      tttcctaaa caccatcga gctcagcaa ttgcgagat 
      atcaaacgt ggagcaggg ggccttcca ggggctgta 
      cagtctcga gtggctgca cctcaacgg aaacaagat 
      gacgacgct gcaaggagc gacctatct gccgaaatc 
      tttgaaagg ggtgcaact ccaggaaaa tccatggga 
      gtgcgactg ccacatact tgaacttca cgcttggct 
      gagggcttt caccatgcc acattccgt cgagccgct 
      ctgtaatgg accaactcg actccggag tcgcacaat 
      taaatcggt gccggtggg agaactcgc ttgtttacc 
      tgaagtttc acccacgat gttctatct cgaaatcgg 
      ggaaggcaa aaatgtcac gcttttatg ccaagttaa 
      cgcaatccc cgaagcgag aatttcctg gacgttcca 
      aggacaatt gctacagaa cgactccat gattgcacc 
      aggtgttca tctcttgta tttcatcga ggagggagc 
      cgtcgaaaa acgcagcga actcttcat ttacaattc 
      taacagtga cgacaacgg aaccttcat atgcaacgc 
      cgaaaatgc agccggact tgtccaagc aaatttcac 
      gattcgagt aatcgtcaa acacgacac tccaccagt 
      cacaaacga actcccctt cgagttcat cctaatcac 
      gctctcagc agcggctgt ctcaatcct cctcttgtt 
      agtcgtaat actcctatc tataataaa atgccaccg 
      aaacgcccg attgaaaaa gaaacgaaa caactcgaa 
      agccgctct cagtaacac aaccaagga caatcttct 
      ccaagaaag cgtcgacga ctactcgga gaaaccccc 
      aactcagga ggaagaaac catgcttta caacacccc 
      tcctgaaga cctccccgt gtcttcaat ccccccgac 
      actcacgcc gtaccagct agagcaaaa ccccgacct 
      catcaacgg cactgaaag tgtggggcg gttcacccc 
      cttgaaaaa acgcgacct gtacgtcta cgcccccga 
      cgttcacct caaccccgt ggggttgct caactcggg 
      ctcgaactg ctacaaaac gctccccta caacaggaa 
      caagcgctc caatgctgc caatccagc tgggaggtt 
      ctcccgcga agctgagtt cctccaacg gacgatgca 
      tccgtcgta tgagcacta cagtgacgt gaggtacac 
      tgcagacgg gtatccggt gaggaccaa cgagggggc 
      tgtggagcc ctgctgctc cacaacgac tacggttag 
      ttggccgtc gtgtgtccc cgcgagttt tgttgcgaa 
      agactgtgt gaagagtgt gggtgctca gactgagga 
      cacaaacac caaatgtga gccgattaa agagacgtt 
      cacggagag tctggatga gggatacga aggggaagg 
      ggcggaggt acatactaa tggggaggc gcgctattt 
      gccctcctg tggttcact aaacaaatt aatattacc 
      atctcccac atgtgtgct tccctacga gtacaaatt 
      taacagata aacagtcta tatcgtcac ggagtaggc 
      tgatactat gaattatgc gccaacaag ctgatttat 
      cagagtcga gctcaaaag ttttgcccc aagattcac 
      aaagttatg gaataagaa aataaatac aggtgatgc 
      gatacaatc gagaagttt tctaattac ataagtcgg 
      cggacaaca aattgaata aaactcgaa tgataaatt 
      atttattgc catttcgat gagctccaa atcgaatga 
      gccttccca ttcaagggc cttttcaat cacgagata 
      aggtatttg cctatttaa agtttcaaa actgtccgg 
      acgaatgaa aaggtattc acaagctcc ctcctgatg 
      gtttaattt ttcaccgct ctaattttc tgctcattt 
      actcaaaca tgcgatgaa atttattgt ttagtaagt 
      tttattgac ttgaatgga gattttaat attcaaact 
      tttgtcgca taaagtgtt tcaacgatg cgagaagtg 
      gaagataaa gacttgaag atattggaa cgcgatttt 
      tatggagtc atagaggtt cagtaatgc tcgtttaaa 
      ctagggaca tgctacttg aaatggatt tagtttaaa 
      tcatgacaa gaaagataa atggaaagc ttttaattt 
      aatatgaag ggtcattgc tgaaatagc caataaaaa 
      ttttacagg acgcataaa aacgcggct ttatttcta 
      ttcctaaaa taaaaaccg ccacttccg tggcaagaa 
      ttttaacta attggtgat cattttatt caattttgt 
      tgttgtaga cgaacttta ataaagtaa aaagctgtt 
      gcaaactat tctgcacta aattccgcc gagatattg 
      ccatgttaa caattttgg cgtctgata ttcgtctct 
      caagacagt gactaaagt cagatgggt actaaacta 
      acgctcatt gacaataat gtccgttac aattacgct 
      gacttgctc catctagca ttaactcta atccattgt 
      aattcatgc ccgacactg ttaattacc aacgtacat 
      tagatcagt acttgtcta ttaagaatc aacgaattc 
      aattaaacc ggattttta tcacaattt ctgcgccaa 
      caggttgtg tacgagtat ttaattaaa atcgggcaa 
      cggtcattt taaaagaat gatgagtgt cgagcttaa 
      cgcggcaac gtttggccg aacaaaatg aatataagg 
      tacatgctg aaagaaatt taattaaca aaagctttg 
      aatataaag gatttatgg ttattaaat cagtgtttg 
      aaaaatcat gtctgtagc agctgaatc aacaaatgg 
      aattgagtc ggtagaacg acctggatg atttctatg 
      attaacttt tgttgcgac tactgtttg gaaatggtt 
      ttaaagaga taacaaatc acatttaat gcaaatatg 
      tttgattgt ggtgtggct ttgcaatta atgtgacac 
      caacaatta aataagcca tgtccacag agatcacta 
      aacgcggaa ttagaccag attaattaa ctaattaaa 
      cgaggaaaa acgcaactc acccaaaat aataaatcg 
      ttaaagcat gaattacct ggaacaact tttttattt 
      ttagttaat ttgttacat tttttctaa attcaggaa 
      acttagttg aaagcagat gatcacaga catcacagt 
      ttagtgacc catgttagt tcagagaca aaatgtcgt 
      gtcagttgc tctattcgt tacaattta gctaaagat 
      agttacgag aggaataag aaaagaaca ccaagcaca 
      aaagactga aatgctgat tgagtgttg atactatga 
      tagaaaaat taaattttc acgataagc taagagcat 
      tgacaacaa taatatttt tactgaact aatagggac 
      ttatttttt cttaaaagc attaaaaaa atcttagat 
      ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY