Status
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Gene information
General information
TC007053 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10529366..10558253
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007053
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08171

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna antennal flagellum unilateral not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum slightly size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007053 RA
      MWVYWLVLL GMLVERSLS CPVSCSCKW KNGKQTVIC 
      SGKSLTDIP DGLDPGTQV LDFSGNFLS NLRRELFSN 
      KQLINLQRI YLSNCQIKI INEKTFKGL SNLVELDLS 
      RNLLETVPT SSFVDCPSL MRLTLSSNP LTVLKRLAF 
      NHLSYLSTL ELDKCKIVE IEDGAFQGL HSLEWLLLE 
      DNGLRTIRG ELPRNLKGV ELRGNPWEC DCHIKELHV 
      WLGRFNVPI SEEPTCSGP PRLASRVIK SIPVSELAC 
      LPDVSPTTF YLELAEGKN VSLQCHVHS IPEASVSWW 
      FRGQILQNN TIVSPGIHL IYFVEEGAE NKRSELFIY 
      NANSEDNGT FICNAENAA GTSQSNFTI KIIIKEDPI 
      VIIVEFPFE CLLAAAIGA VVLALLIIS GIIISILRC 
      KRRKEPLQG KTEEDTNPK PTPDDDTLY VCEDHPVPN 
      HQSPTLLFQ DQNPDLINH RWLDSEGYP LDYGLPKLP 
      TNYYRTLPS RPKRQSLQR PRYSQEAEF LAYDHYHTD 
      VRYTADGYP LPSNDGAPC CSIQWPVCV PANLHMVNN 
      NPNEMVYEP PSGIIKRCV GAQTEDEKI DSNEAGNEA 
      MTESPDEGY EGEPAVV
    CDS sequence
    • >TC007053 RA
      atgtgggtt tattggttg gtcttgctt ggaatgctc 
      gttgaaaga agcctctca tgccccgtc tcctgttcc 
      tgcaagtgg aaaaatggc aagcaaact gtgatctgt 
      tccggtaaa agtctgacc gacatcccc gacggtttg 
      gatccgggt actcaagtc ctggatttc tccggcaac 
      ttcctcagc aatctccgt cgcgaacta ttcagcaac 
      aaacaactg attaacttg cagcgcatt tatttgtcc 
      aattgtcaa ataaaaata ataaacgag aaaactttc 
      aagggtttg tcaaacttg gtcgagctc gatctctct 
      cggaatttg cttgaaacg gtgcctacg agttcgttt 
      gtggactgt ccttcttta atgagacta actctaagc 
      tcgaaccct ttaaccgtt ttaaaaaga ctggctttc 
      aatcattta tcgtatctc agcacactc gagctcgat 
      aaatgcaaa attgtggag attgaggac ggggctttt 
      caaggcttg cacagtctg gagtggcta ctcctcgaa 
      gataatggt ttgaggacg attcggggg gaattaccc 
      aggaatttg aaaggtgtg gagttgcgg gggaatccc 
      tgggagtgc gactgtcac attaaggag ttgcacgtt 
      tggctgggg cgctttaac gtgcccatc tccgaagag 
      ccgacttgt tcagggcct cctcgactc gcttcccgg 
      gttattaaa tcgataccg gtctctgaa ctcgcttgt 
      cttcctgac gtatccccc acaacgttt tatctggag 
      ctggccgaa ggcaagaac gtatctctg caatgtcac 
      gtgcattct atcccggaa gcttccgtc tcctggtgg 
      ttccgcgga cagatcctc cagaacaat accatcgta 
      tccccggga atccacttg atttacttc gtggaggag 
      ggcgcggag aacaagcgc agtgaactg ttcatctat 
      aacgccaac tccgaagac aatggtaca tttatctgc 
      aacgccgaa aacgccgct ggaacatcg caatcgaat 
      tttacaatc aagataatt attaaagaa gatccgatt 
      gtaattatc gttgagttt cctttcgaa tgtctccta 
      gcggcggcg atcggagct gtcgtttta gccctccta 
      ataatctcc ggtatcatc atatcgatc ctccgatgc 
      aaacggcgg aaagagccc ctgcaagga aaaaccgaa 
      gaggacact aaccccaag cccaccccc gacgacgac 
      acactttac gtctgcgag gatcacccg gttcctaat 
      caccaaagc ccaactttg ctcttccaa gaccaaaac 
      cccgacctt atcaaccac cgatggctc gactccgag 
      ggctacccc ctcgactac ggccttccg aaactcccg 
      acgaattac taccgaacg ctgccgagt aggcccaaa 
      cgacaatca ctccaaaga ccgagatac tcgcaagaa 
      gccgaattc ctggcttac gatcattac cacaccgat 
      gtccgttac acagctgac ggatatccg ttgccgtca 
      aatgacgga gctccttgc tgttcgatt cagtggccg 
      gtttgtgtg cccgcgaat ctacacatg gttaataat 
      aatcccaat gagatggtt tatgagccg ccgagcgga 
      attatcaaa cgctgcgtc ggagctcaa accgaagat 
      gagaagatt gattccaat gaggctgga aatgaagcg 
      atgaccgaa agtccagat gagggatat gagggagaa 
      ccggccgtc gtatag
    mRNA sequence
    • >TC007053 RA
      ttggtagca ctggagcca ccgcgacct taactttga 
      atctcaatt agaaatgtt tgtgtgttt tgaacaatt 
      tgtattggt ttgtcgagc aatgtgagc tttcgaggg 
      tcgagtgat ggaaaatgt gggtttatt ggttggtct 
      tgcttggaa tgctcgttg aaagaagcc tctcatgcc 
      ccgtctcct gttcctgca agtggaaaa atggcaagc 
      aaactgtga tctgttccg gtaaaagtc tgaccgaca 
      tccccgacg gtttggatc cgggtactc aagtcctgg 
      atttctccg gcaacttcc tcagcaatc tccgtcgcg 
      aactattca gcaacaaac aactgatta acttgcagc 
      gcatttatt tgtccaatt gtcaaataa aaataataa 
      acgagaaaa ctttcaagg gtttgtcaa acttggtcg 
      agctcgatc tctctcgga atttgcttg aaacggtgc 
      ctacgagtt cgtttgtgg actgtcctt ctttaatga 
      gactaactc taagctcga accctttaa ccgttttaa 
      aaagactgg ctttcaatc atttatcgt atctcagca 
      cactcgagc tcgataaat gcaaaattg tggagattg 
      aggacgggg cttttcaag gcttgcaca gtctggagt 
      ggctactcc tcgaagata atggtttga ggacgattc 
      ggggggaat tacccagga atttgaaag gtgtggagt 
      tgcggggga atccctggg agtgcgact gtcacatta 
      aggagttgc acgtttggc tggggcgct ttaacgtgc 
      ccatctccg aagagccga cttgttcag ggcctcctc 
      gactcgctt cccgggtta ttaaatcga taccggtct 
      ctgaactcg cttgtcttc ctgacgtat cccccacaa 
      cgttttatc tggagctgg ccgaaggca agaacgtat 
      ctctgcaat gtcacgtgc attctatcc cggaagctt 
      ccgtctcct ggtggttcc gcggacaga tcctccaga 
      acaatacca tcgtatccc cgggaatcc acttgattt 
      acttcgtgg aggagggcg cggagaaca agcgcagtg 
      aactgttca tctataacg ccaactccg aagacaatg 
      gtacattta tctgcaacg ccgaaaacg ccgctggaa 
      catcgcaat cgaatttta caatcaaga taattatta 
      aagaagatc cgattgtaa ttatcgttg agtttcctt 
      tcgaatgtc tcctagcgg cggcgatcg gagctgtcg 
      ttttagccc tcctaataa tctccggta tcatcatat 
      cgatcctcc gatgcaaac ggcggaaag agcccctgc 
      aaggaaaaa ccgaagagg acactaacc ccaagccca 
      cccccgacg acgacacac tttacgtct gcgaggatc 
      acccggttc ctaatcacc aaagcccaa ctttgctct 
      tccaagacc aaaaccccg accttatca accaccgat 
      ggctcgact ccgagggct accccctcg actacggcc 
      ttccgaaac tcccgacga attactacc gaacgctgc 
      cgagtaggc ccaaacgac aatcactcc aaagaccga 
      gatactcgc aagaagccg aattcctgg cttacgatc 
      attaccaca ccgatgtcc gttacacag ctgacggat 
      atccgttgc cgtcaaatg acggagctc cttgctgtt 
      cgattcagt ggccggttt gtgtgcccg cgaatctac 
      acatggtta ataataatc ccaatgaga tggtttatg 
      agccgccga gcggaatta tcaaacgct gcgtcggag 
      ctcaaaccg aagatgaga agattgatt ccaatgagg 
      ctggaaatg aagcgatga ccgaaagtc cagatgagg 
      gatatgagg gagaaccgg ccgtcgtat agtgttaag 
      agagcgcga atgcaatca cagggtaat gttttagtt 
      aggttttgt atcccgatt tctttatct agactgtgt 
      atatactgt agagtttta gaatttttt tcgtaataa 
      attacttaa tcggaagcg tttccaaat ttatgcaat 
      aaatcacga tattaccat aatgtgtag agtgaatat 
      tgttcaaca agctctatt gtcagcgtc gaatacagt 
      tttttccca att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY