General information | |||
TC007053 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 10529366..10558253 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC007053 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08171 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
antenna antennal flagellum unilateral not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
labrum slightly size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007053 RA
MWVYWLVLL GMLVERSLS CPVSCSCKW KNGKQTVIC SGKSLTDIP DGLDPGTQV LDFSGNFLS NLRRELFSN KQLINLQRI YLSNCQIKI INEKTFKGL SNLVELDLS RNLLETVPT SSFVDCPSL MRLTLSSNP LTVLKRLAF NHLSYLSTL ELDKCKIVE IEDGAFQGL HSLEWLLLE DNGLRTIRG ELPRNLKGV ELRGNPWEC DCHIKELHV WLGRFNVPI SEEPTCSGP PRLASRVIK SIPVSELAC LPDVSPTTF YLELAEGKN VSLQCHVHS IPEASVSWW FRGQILQNN TIVSPGIHL IYFVEEGAE NKRSELFIY NANSEDNGT FICNAENAA GTSQSNFTI KIIIKEDPI VIIVEFPFE CLLAAAIGA VVLALLIIS GIIISILRC KRRKEPLQG KTEEDTNPK PTPDDDTLY VCEDHPVPN HQSPTLLFQ DQNPDLINH RWLDSEGYP LDYGLPKLP TNYYRTLPS RPKRQSLQR PRYSQEAEF LAYDHYHTD VRYTADGYP LPSNDGAPC CSIQWPVCV PANLHMVNN NPNEMVYEP PSGIIKRCV GAQTEDEKI DSNEAGNEA MTESPDEGY EGEPAVV
- >TC007053 RA
atgtgggtt tattggttg gtcttgctt ggaatgctc gttgaaaga agcctctca tgccccgtc tcctgttcc tgcaagtgg aaaaatggc aagcaaact gtgatctgt tccggtaaa agtctgacc gacatcccc gacggtttg gatccgggt actcaagtc ctggatttc tccggcaac ttcctcagc aatctccgt cgcgaacta ttcagcaac aaacaactg attaacttg cagcgcatt tatttgtcc aattgtcaa ataaaaata ataaacgag aaaactttc aagggtttg tcaaacttg gtcgagctc gatctctct cggaatttg cttgaaacg gtgcctacg agttcgttt gtggactgt ccttcttta atgagacta actctaagc tcgaaccct ttaaccgtt ttaaaaaga ctggctttc aatcattta tcgtatctc agcacactc gagctcgat aaatgcaaa attgtggag attgaggac ggggctttt caaggcttg cacagtctg gagtggcta ctcctcgaa gataatggt ttgaggacg attcggggg gaattaccc aggaatttg aaaggtgtg gagttgcgg gggaatccc tgggagtgc gactgtcac attaaggag ttgcacgtt tggctgggg cgctttaac gtgcccatc tccgaagag ccgacttgt tcagggcct cctcgactc gcttcccgg gttattaaa tcgataccg gtctctgaa ctcgcttgt cttcctgac gtatccccc acaacgttt tatctggag ctggccgaa ggcaagaac gtatctctg caatgtcac gtgcattct atcccggaa gcttccgtc tcctggtgg ttccgcgga cagatcctc cagaacaat accatcgta tccccggga atccacttg atttacttc gtggaggag ggcgcggag aacaagcgc agtgaactg ttcatctat aacgccaac tccgaagac aatggtaca tttatctgc aacgccgaa aacgccgct ggaacatcg caatcgaat tttacaatc aagataatt attaaagaa gatccgatt gtaattatc gttgagttt cctttcgaa tgtctccta gcggcggcg atcggagct gtcgtttta gccctccta ataatctcc ggtatcatc atatcgatc ctccgatgc aaacggcgg aaagagccc ctgcaagga aaaaccgaa gaggacact aaccccaag cccaccccc gacgacgac acactttac gtctgcgag gatcacccg gttcctaat caccaaagc ccaactttg ctcttccaa gaccaaaac cccgacctt atcaaccac cgatggctc gactccgag ggctacccc ctcgactac ggccttccg aaactcccg acgaattac taccgaacg ctgccgagt aggcccaaa cgacaatca ctccaaaga ccgagatac tcgcaagaa gccgaattc ctggcttac gatcattac cacaccgat gtccgttac acagctgac ggatatccg ttgccgtca aatgacgga gctccttgc tgttcgatt cagtggccg gtttgtgtg cccgcgaat ctacacatg gttaataat aatcccaat gagatggtt tatgagccg ccgagcgga attatcaaa cgctgcgtc ggagctcaa accgaagat gagaagatt gattccaat gaggctgga aatgaagcg atgaccgaa agtccagat gagggatat gagggagaa ccggccgtc gtatag
- >TC007053 RA
ttggtagca ctggagcca ccgcgacct taactttga atctcaatt agaaatgtt tgtgtgttt tgaacaatt tgtattggt ttgtcgagc aatgtgagc tttcgaggg tcgagtgat ggaaaatgt gggtttatt ggttggtct tgcttggaa tgctcgttg aaagaagcc tctcatgcc ccgtctcct gttcctgca agtggaaaa atggcaagc aaactgtga tctgttccg gtaaaagtc tgaccgaca tccccgacg gtttggatc cgggtactc aagtcctgg atttctccg gcaacttcc tcagcaatc tccgtcgcg aactattca gcaacaaac aactgatta acttgcagc gcatttatt tgtccaatt gtcaaataa aaataataa acgagaaaa ctttcaagg gtttgtcaa acttggtcg agctcgatc tctctcgga atttgcttg aaacggtgc ctacgagtt cgtttgtgg actgtcctt ctttaatga gactaactc taagctcga accctttaa ccgttttaa aaagactgg ctttcaatc atttatcgt atctcagca cactcgagc tcgataaat gcaaaattg tggagattg aggacgggg cttttcaag gcttgcaca gtctggagt ggctactcc tcgaagata atggtttga ggacgattc ggggggaat tacccagga atttgaaag gtgtggagt tgcggggga atccctggg agtgcgact gtcacatta aggagttgc acgtttggc tggggcgct ttaacgtgc ccatctccg aagagccga cttgttcag ggcctcctc gactcgctt cccgggtta ttaaatcga taccggtct ctgaactcg cttgtcttc ctgacgtat cccccacaa cgttttatc tggagctgg ccgaaggca agaacgtat ctctgcaat gtcacgtgc attctatcc cggaagctt ccgtctcct ggtggttcc gcggacaga tcctccaga acaatacca tcgtatccc cgggaatcc acttgattt acttcgtgg aggagggcg cggagaaca agcgcagtg aactgttca tctataacg ccaactccg aagacaatg gtacattta tctgcaacg ccgaaaacg ccgctggaa catcgcaat cgaatttta caatcaaga taattatta aagaagatc cgattgtaa ttatcgttg agtttcctt tcgaatgtc tcctagcgg cggcgatcg gagctgtcg ttttagccc tcctaataa tctccggta tcatcatat cgatcctcc gatgcaaac ggcggaaag agcccctgc aaggaaaaa ccgaagagg acactaacc ccaagccca cccccgacg acgacacac tttacgtct gcgaggatc acccggttc ctaatcacc aaagcccaa ctttgctct tccaagacc aaaaccccg accttatca accaccgat ggctcgact ccgagggct accccctcg actacggcc ttccgaaac tcccgacga attactacc gaacgctgc cgagtaggc ccaaacgac aatcactcc aaagaccga gatactcgc aagaagccg aattcctgg cttacgatc attaccaca ccgatgtcc gttacacag ctgacggat atccgttgc cgtcaaatg acggagctc cttgctgtt cgattcagt ggccggttt gtgtgcccg cgaatctac acatggtta ataataatc ccaatgaga tggtttatg agccgccga gcggaatta tcaaacgct gcgtcggag ctcaaaccg aagatgaga agattgatt ccaatgagg ctggaaatg aagcgatga ccgaaagtc cagatgagg gatatgagg gagaaccgg ccgtcgtat agtgttaag agagcgcga atgcaatca cagggtaat gttttagtt aggttttgt atcccgatt tctttatct agactgtgt atatactgt agagtttta gaatttttt tcgtaataa attacttaa tcggaagcg tttccaaat ttatgcaat aaatcacga tattaccat aatgtgtag agtgaatat tgttcaaca agctctatt gtcagcgtc gaatacagt tttttccca att
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
