General information | |||
TC031474
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 3835284..3843408 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031474 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08169 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva segment size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva ventral not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
mandible shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
Comment: with femur |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031474 RA
MVTRPGNLY KTPPGSRGT SPARSLNRS TKPSPVQSP NRTSRSPLR TTSKKIQSS PDRQRKTPE KVLIRSRGN SPAKSRDSL PGSRGSDFG SIERLDDDN NLQRIITSY NQTEDNINV VVRVRPLNN REKKSGDTS VIQFPGNGQ VLVDGIPGG PSGGQKPKL FSYNVVFEP AATQEDVLQ FSGMKRLIE MALEGFRCT CFCYGQTGS GKTHTLTGP PGLFGVRPA PFSDKHGLV FRSFLYLFQ LIQQKTDVH FVLKASYLE IYNEKVIDL LNPGSARKP LAVRWSKKA RGFFVENLF TVDCEELDD LIAVLEEGL RNRAIGKHN MNDFSSRSH TILTVYITS EEPAEGGVF LSRSGKINF VDLAGSEMT KKTQSEGKT LEEANNINK SLMVLGYCI SSLSDSKRR NSHIPYRDS KLTKLLADS LAGNGVTLM IACISPARS NISETLNTL RYAARAKKI RTKPIVLMD PREALILSL KREVNVLQS ENEHLRSAL NVYSGSTAG DLPTERRIV VTPPHVDLE KLAELEGPQ LAELVRLYM TENEALRRE NSELYSSRE ILQRDHELV CRENERLLK KLEEVNSVC CRSPLIPAR PAFSAELLN SIDSESTTN IWSDGDKSS HSRSPQQIP QSIQKELDK RQIKTEIQT PRRGHRRNK SLEESSSDS KVVRVKSSE SDGGPVLIN RSGTFDSNS PERERQLAG RRFSVAKPD ENEPSMNGA RRKLEKNSH IFGSVISLT DEEDI
- >TC031474 RA
atggtgacg cgtcctgga aacctgtac aaaactcct ccaggatca cgtggcacc agtcctgcc aggagtctg aacaggagc actaaaccc agtccggtc cagtcaccg aatcggact tcgagaagt ccactcagg actacctcg aagaagatt caaagcagt cccgacaga cagagaaaa actccggag aaagttttg ataaggtcg aggggcaac agtccggcg aaaagtcgg gacagtttg cccggttca agagggagc gattttggt agcatagag cgactggat gacgataac aatctacaa agaataatt acaagttac aatcaaacg gaagataat attaatgta gtcgtcagg gtgcgacca ctcaataac cgtgaaaaa aaatcgggt gatacttct gtgatacaa ttccctggc aatggtcaa gtcttggtg gatggaata ccaggcggt ccaagtggc ggtcagaaa cctaaactt ttctcatat aacgtagtt ttcgagcct gcggccact caggaagac gttttgcag ttttcaggc atgaaaaga ctcattgaa atggcgctt gaagggttc aggtgtacg tgtttttgt tatggacaa actggaagc ggaaaaaca cacacactg acaggacct cctggcctt ttcggggtg agaccggcc ccttttagt gataaacac ggccttgtt tttcgatcg ttcctttat ttgtttcaa ctgatacaa caaaaaacc gacgtacat ttcgtcctc aaagccagc tatctggag atttacaac gaaaaggta attgattta ttaaatcct ggaagcgca aggaagccc ctagcagtc cggtggagc aaaaaagca cgcggtttt ttcgttgag aatcttttt acggtcgac tgtgaagag ctcgacgac ttaattgca gttctggaa gaaggatta agaaatcgt gctataggg aagcacaac atgaatgat ttttccagc cgaagtcat acgatttta actgtttat atcacgtct gaagaacca gctgaagga ggtgttttt ctctcgagg tctgggaaa ataaatttc gttgatttg gcgggaagt gaaatgacc aaaaagaca caaagtgag ggcaaaacg ttagaagag gcgaataat attaataaa agtctgatg gtgttaggg tactgcata tcgtcgtta agtgattcc aagcgaagg aactcgcat attccttat cgagattca aaactgacc aagctcctt gcagattca ttggcagga aatggcgta acactgatg attgcttgc atttctcct gccagatcc aacatcagc gagaccctc aacacgctg cgctatgcc gctcgagcg aagaaaatt cgaacaaag cctattgtc ctgatggac cctcgagaa gcgctcatt ctgagcctg aaacgagaa gtgaatgtc ctgcagagc gaaaacgag catctgcgc tcggcgctc aacgtctac tcagggtca acagcagga gatttacca acagagagg agaatagtg gtaacccca ccccacgtg gatttggaa aaactggcc gaactcgaa ggcccccag ctcgccgaa ctcgtccgc ttatacatg accgaaaac gaagctctc cgacgcgaa aactccgag ctctactct tcccgcgaa atcctacaa agagaccac gaactcgtc tgccgggaa aacgagcgt ttactaaag aaactcgaa gaagtgaac tctgtatgt tgtaggtct cccctgatt ccagcccgg cccgcattt tccgccgaa ctcctcaac agcatcgac tccgaatcc acgacaaac atttggtcg gacggagac aaatcttca cacagccga tcgccccaa caaattccg caatcgatt cagaaggag ttggacaag agacaaatt aaaacagag attcagacg cctagacgc ggacaccgc aggaacaag tccttggaa gaaagctcg agcgattcg aaagtagtt cgagtgaaa agctccgag tcagacgga ggccccgtt ctgataaat cgttccggc actttcgac tcgaatagt cccgaaaga gaacgccaa ctggcaggt aggcggttt tctgtggca aaaccggac gaaaatgag ccgtcaatg aatggagca cgacgcaaa ttagagaag aattcacat atttttgga agcgttatt tccttaacg gatgaagaa gacatttaa
- >TC031474 RA
cgtcatttg tattaattg gacgacgcg tagcgacgg ccggcgtcc aatgtcaac cgtttgcgt gttttgtag cgtcgcggg cgtcagttg taacgcgtt acacgtgct atgaacact ttaacaaca aactgttcg ttgtatttc caatacaca cacgttaat tacctccag tcgtgtata tttacagtt tatgactaa caatgataa cgatgcaaa ttgcctacc cgatttaaa gttttattt gcaatgtga aaatttaaa gaaaatgag tgaatgact tgcgaaaca cgaaaatgg tgacgcgtc ctggaaacc tgtacaaaa ctcctccag gatcacgtg gcaccagtc ctgccagga gtctgaaca ggagcacta aacccagtc cggtccagt caccgaatc ggacttcga gaagtccac tcaggacta cctcgaaga agattcaaa gcagtcccg acagacaga gaaaaactc cggagaaag ttttgataa ggtcgaggg gcaacagtc cggcgaaaa gtcgggaca gtttgcccg gttcaagag ggagcgatt ttggtagca tagagcgac tggatgacg ataacaatc tacaaagaa taattacaa gttacaatc aaacggaag ataatatta atgtagtcg tcagggtgc gaccactca ataaccgtg aaaaaaaat cgggtgata cttctgtga tacaattcc ctggcaatg gtcaagtct tggtggatg gaataccag gcggtccaa gtggcggtc agaaaccta aacttttct catataacg tagttttcg agcctgcgg ccactcagg aagacgttt tgcagtttt caggcatga aaagactca ttgaaatgg cgcttgaag ggttcaggt gtacgtgtt tttgttatg gacaaactg gaagcggaa aaacacaca cactgacag gacctcctg gccttttcg gggtgagac cggcccctt ttagtgata aacacggcc ttgtttttc gatcgttcc tttatttgt ttcaactga tacaacaaa aaaccgacg tacatttcg tcctcaaag ccagctatc tggagattt acaacgaaa aggtaattg atttattaa atcctggaa gcgcaagga agcccctag cagtccggt ggagcaaaa aagcacgcg gttttttcg ttgagaatc tttttacgg tcgactgtg aagagctcg acgacttaa ttgcagttc tggaagaag gattaagaa atcgtgcta tagggaagc acaacatga atgattttt ccagccgaa gtcatacga ttttaactg tttatatca cgtctgaag aaccagctg aaggaggtg tttttctct cgaggtctg ggaaaataa atttcgttg atttggcgg gaagtgaaa tgaccaaaa agacacaaa gtgagggca aaacgttag aagaggcga ataatatta ataaaagtc tgatggtgt tagggtact gcatatcgt cgttaagtg attccaagc gaaggaact cgcatattc cttatcgag attcaaaac tgaccaagc tccttgcag attcattgg caggaaatg gcgtaacac tgatgattg cttgcattt ctcctgcca gatccaaca tcagcgaga ccctcaaca cgctgcgct atgccgctc gagcgaaga aaattcgaa caaagccta ttgtcctga tggaccctc gagaagcgc tcattctga gcctgaaac gagaagtga atgtcctgc agagcgaaa acgagcatc tgcgctcgg cgctcaacg tctactcag ggtcaacag caggagatt taccaacag agaggagaa tagtggtaa ccccacccc acgtggatt tggaaaaac tggccgaac tcgaaggcc cccagctcg ccgaactcg tccgcttat acatgaccg aaaacgaag ctctccgac gcgaaaact ccgagctct actcttccc gcgaaatcc tacaaagag accacgaac tcgtctgcc gggaaaacg agcgtttac taaagaaac tcgaagaag tgaactctg tatgttgta ggtctcccc tgattccag cccggcccg cattttccg ccgaactcc tcaacagca tcgactccg aatccacga caaacattt ggtcggacg gagacaaat cttcacaca gccgatcgc cccaacaaa ttccgcaat cgattcaga aggagttgg acaagagac aaattaaaa cagagattc agacgccta gacgcggac accgcagga acaagtcct tggaagaaa gctcgagcg attcgaaag tagttcgag tgaaaagct ccgagtcag acggaggcc ccgttctga taaatcgtt ccggcactt tcgactcga atagtcccg aaagagaac gccaactgg caggtaggc ggttttctg tggcaaaac cggacgaaa atgagccgt caatgaatg gagcacgac gcaaattag agaagaatt cacatattt ttggaagcg ttatttcct taacggatg aagaagaca tttaattaa tatagctcg agtgatact gtagcaata cataatctt attttagta cgttctccc atattgtac caatagtta atttgaaga cacactgta gaattaaac acttggaat attcaaatt gttactatt aaaacaagg tacaaatat acattattt tactag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
