Status
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Gene information
General information
TC031474
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011512
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3835284..3843408
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031474
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08169

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva segment size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva ventral not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    mandible shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: with femur
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031474 RA
      MVTRPGNLY KTPPGSRGT SPARSLNRS TKPSPVQSP 
      NRTSRSPLR TTSKKIQSS PDRQRKTPE KVLIRSRGN 
      SPAKSRDSL PGSRGSDFG SIERLDDDN NLQRIITSY 
      NQTEDNINV VVRVRPLNN REKKSGDTS VIQFPGNGQ 
      VLVDGIPGG PSGGQKPKL FSYNVVFEP AATQEDVLQ 
      FSGMKRLIE MALEGFRCT CFCYGQTGS GKTHTLTGP 
      PGLFGVRPA PFSDKHGLV FRSFLYLFQ LIQQKTDVH 
      FVLKASYLE IYNEKVIDL LNPGSARKP LAVRWSKKA 
      RGFFVENLF TVDCEELDD LIAVLEEGL RNRAIGKHN 
      MNDFSSRSH TILTVYITS EEPAEGGVF LSRSGKINF 
      VDLAGSEMT KKTQSEGKT LEEANNINK SLMVLGYCI 
      SSLSDSKRR NSHIPYRDS KLTKLLADS LAGNGVTLM 
      IACISPARS NISETLNTL RYAARAKKI RTKPIVLMD 
      PREALILSL KREVNVLQS ENEHLRSAL NVYSGSTAG 
      DLPTERRIV VTPPHVDLE KLAELEGPQ LAELVRLYM 
      TENEALRRE NSELYSSRE ILQRDHELV CRENERLLK 
      KLEEVNSVC CRSPLIPAR PAFSAELLN SIDSESTTN 
      IWSDGDKSS HSRSPQQIP QSIQKELDK RQIKTEIQT 
      PRRGHRRNK SLEESSSDS KVVRVKSSE SDGGPVLIN 
      RSGTFDSNS PERERQLAG RRFSVAKPD ENEPSMNGA 
      RRKLEKNSH IFGSVISLT DEEDI
    CDS sequence
    • >TC031474 RA
      atggtgacg cgtcctgga aacctgtac aaaactcct 
      ccaggatca cgtggcacc agtcctgcc aggagtctg 
      aacaggagc actaaaccc agtccggtc cagtcaccg 
      aatcggact tcgagaagt ccactcagg actacctcg 
      aagaagatt caaagcagt cccgacaga cagagaaaa 
      actccggag aaagttttg ataaggtcg aggggcaac 
      agtccggcg aaaagtcgg gacagtttg cccggttca 
      agagggagc gattttggt agcatagag cgactggat 
      gacgataac aatctacaa agaataatt acaagttac 
      aatcaaacg gaagataat attaatgta gtcgtcagg 
      gtgcgacca ctcaataac cgtgaaaaa aaatcgggt 
      gatacttct gtgatacaa ttccctggc aatggtcaa 
      gtcttggtg gatggaata ccaggcggt ccaagtggc 
      ggtcagaaa cctaaactt ttctcatat aacgtagtt 
      ttcgagcct gcggccact caggaagac gttttgcag 
      ttttcaggc atgaaaaga ctcattgaa atggcgctt 
      gaagggttc aggtgtacg tgtttttgt tatggacaa 
      actggaagc ggaaaaaca cacacactg acaggacct 
      cctggcctt ttcggggtg agaccggcc ccttttagt 
      gataaacac ggccttgtt tttcgatcg ttcctttat 
      ttgtttcaa ctgatacaa caaaaaacc gacgtacat 
      ttcgtcctc aaagccagc tatctggag atttacaac 
      gaaaaggta attgattta ttaaatcct ggaagcgca 
      aggaagccc ctagcagtc cggtggagc aaaaaagca 
      cgcggtttt ttcgttgag aatcttttt acggtcgac 
      tgtgaagag ctcgacgac ttaattgca gttctggaa 
      gaaggatta agaaatcgt gctataggg aagcacaac 
      atgaatgat ttttccagc cgaagtcat acgatttta 
      actgtttat atcacgtct gaagaacca gctgaagga 
      ggtgttttt ctctcgagg tctgggaaa ataaatttc 
      gttgatttg gcgggaagt gaaatgacc aaaaagaca 
      caaagtgag ggcaaaacg ttagaagag gcgaataat 
      attaataaa agtctgatg gtgttaggg tactgcata 
      tcgtcgtta agtgattcc aagcgaagg aactcgcat 
      attccttat cgagattca aaactgacc aagctcctt 
      gcagattca ttggcagga aatggcgta acactgatg 
      attgcttgc atttctcct gccagatcc aacatcagc 
      gagaccctc aacacgctg cgctatgcc gctcgagcg 
      aagaaaatt cgaacaaag cctattgtc ctgatggac 
      cctcgagaa gcgctcatt ctgagcctg aaacgagaa 
      gtgaatgtc ctgcagagc gaaaacgag catctgcgc 
      tcggcgctc aacgtctac tcagggtca acagcagga 
      gatttacca acagagagg agaatagtg gtaacccca 
      ccccacgtg gatttggaa aaactggcc gaactcgaa 
      ggcccccag ctcgccgaa ctcgtccgc ttatacatg 
      accgaaaac gaagctctc cgacgcgaa aactccgag 
      ctctactct tcccgcgaa atcctacaa agagaccac 
      gaactcgtc tgccgggaa aacgagcgt ttactaaag 
      aaactcgaa gaagtgaac tctgtatgt tgtaggtct 
      cccctgatt ccagcccgg cccgcattt tccgccgaa 
      ctcctcaac agcatcgac tccgaatcc acgacaaac 
      atttggtcg gacggagac aaatcttca cacagccga 
      tcgccccaa caaattccg caatcgatt cagaaggag 
      ttggacaag agacaaatt aaaacagag attcagacg 
      cctagacgc ggacaccgc aggaacaag tccttggaa 
      gaaagctcg agcgattcg aaagtagtt cgagtgaaa 
      agctccgag tcagacgga ggccccgtt ctgataaat 
      cgttccggc actttcgac tcgaatagt cccgaaaga 
      gaacgccaa ctggcaggt aggcggttt tctgtggca 
      aaaccggac gaaaatgag ccgtcaatg aatggagca 
      cgacgcaaa ttagagaag aattcacat atttttgga 
      agcgttatt tccttaacg gatgaagaa gacatttaa
    mRNA sequence
    • >TC031474 RA
      cgtcatttg tattaattg gacgacgcg tagcgacgg 
      ccggcgtcc aatgtcaac cgtttgcgt gttttgtag 
      cgtcgcggg cgtcagttg taacgcgtt acacgtgct 
      atgaacact ttaacaaca aactgttcg ttgtatttc 
      caatacaca cacgttaat tacctccag tcgtgtata 
      tttacagtt tatgactaa caatgataa cgatgcaaa 
      ttgcctacc cgatttaaa gttttattt gcaatgtga 
      aaatttaaa gaaaatgag tgaatgact tgcgaaaca 
      cgaaaatgg tgacgcgtc ctggaaacc tgtacaaaa 
      ctcctccag gatcacgtg gcaccagtc ctgccagga 
      gtctgaaca ggagcacta aacccagtc cggtccagt 
      caccgaatc ggacttcga gaagtccac tcaggacta 
      cctcgaaga agattcaaa gcagtcccg acagacaga 
      gaaaaactc cggagaaag ttttgataa ggtcgaggg 
      gcaacagtc cggcgaaaa gtcgggaca gtttgcccg 
      gttcaagag ggagcgatt ttggtagca tagagcgac 
      tggatgacg ataacaatc tacaaagaa taattacaa 
      gttacaatc aaacggaag ataatatta atgtagtcg 
      tcagggtgc gaccactca ataaccgtg aaaaaaaat 
      cgggtgata cttctgtga tacaattcc ctggcaatg 
      gtcaagtct tggtggatg gaataccag gcggtccaa 
      gtggcggtc agaaaccta aacttttct catataacg 
      tagttttcg agcctgcgg ccactcagg aagacgttt 
      tgcagtttt caggcatga aaagactca ttgaaatgg 
      cgcttgaag ggttcaggt gtacgtgtt tttgttatg 
      gacaaactg gaagcggaa aaacacaca cactgacag 
      gacctcctg gccttttcg gggtgagac cggcccctt 
      ttagtgata aacacggcc ttgtttttc gatcgttcc 
      tttatttgt ttcaactga tacaacaaa aaaccgacg 
      tacatttcg tcctcaaag ccagctatc tggagattt 
      acaacgaaa aggtaattg atttattaa atcctggaa 
      gcgcaagga agcccctag cagtccggt ggagcaaaa 
      aagcacgcg gttttttcg ttgagaatc tttttacgg 
      tcgactgtg aagagctcg acgacttaa ttgcagttc 
      tggaagaag gattaagaa atcgtgcta tagggaagc 
      acaacatga atgattttt ccagccgaa gtcatacga 
      ttttaactg tttatatca cgtctgaag aaccagctg 
      aaggaggtg tttttctct cgaggtctg ggaaaataa 
      atttcgttg atttggcgg gaagtgaaa tgaccaaaa 
      agacacaaa gtgagggca aaacgttag aagaggcga 
      ataatatta ataaaagtc tgatggtgt tagggtact 
      gcatatcgt cgttaagtg attccaagc gaaggaact 
      cgcatattc cttatcgag attcaaaac tgaccaagc 
      tccttgcag attcattgg caggaaatg gcgtaacac 
      tgatgattg cttgcattt ctcctgcca gatccaaca 
      tcagcgaga ccctcaaca cgctgcgct atgccgctc 
      gagcgaaga aaattcgaa caaagccta ttgtcctga 
      tggaccctc gagaagcgc tcattctga gcctgaaac 
      gagaagtga atgtcctgc agagcgaaa acgagcatc 
      tgcgctcgg cgctcaacg tctactcag ggtcaacag 
      caggagatt taccaacag agaggagaa tagtggtaa 
      ccccacccc acgtggatt tggaaaaac tggccgaac 
      tcgaaggcc cccagctcg ccgaactcg tccgcttat 
      acatgaccg aaaacgaag ctctccgac gcgaaaact 
      ccgagctct actcttccc gcgaaatcc tacaaagag 
      accacgaac tcgtctgcc gggaaaacg agcgtttac 
      taaagaaac tcgaagaag tgaactctg tatgttgta 
      ggtctcccc tgattccag cccggcccg cattttccg 
      ccgaactcc tcaacagca tcgactccg aatccacga 
      caaacattt ggtcggacg gagacaaat cttcacaca 
      gccgatcgc cccaacaaa ttccgcaat cgattcaga 
      aggagttgg acaagagac aaattaaaa cagagattc 
      agacgccta gacgcggac accgcagga acaagtcct 
      tggaagaaa gctcgagcg attcgaaag tagttcgag 
      tgaaaagct ccgagtcag acggaggcc ccgttctga 
      taaatcgtt ccggcactt tcgactcga atagtcccg 
      aaagagaac gccaactgg caggtaggc ggttttctg 
      tggcaaaac cggacgaaa atgagccgt caatgaatg 
      gagcacgac gcaaattag agaagaatt cacatattt 
      ttggaagcg ttatttcct taacggatg aagaagaca 
      tttaattaa tatagctcg agtgatact gtagcaata 
      cataatctt attttagta cgttctccc atattgtac 
      caatagtta atttgaaga cacactgta gaattaaac 
      acttggaat attcaaatt gttactatt aaaacaagg 
      tacaaatat acattattt tactag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY