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Gene information
General information
TC008353 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8960160..8967175
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008353
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08164

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 4

    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008353 RA
      MVVCPADHG LQADIVFVI EGTAVNGAY INDLKTNYI 
      IPSLEYFSQ GSIDESSFL SEGINSYYG IVVYQAADC 
      LPHPSSDTI GPFVSPSKV LNAIDKLEL IGGKGESHA 
      NIAEGLATT LQCFDELQQ KRDNNGNIQ KHCILVCNS 
      PPYSLGVFE THAYTGKTA EQLAAILQE KNINLSIIS 
      PRKIPSLYK LFEKAGGDL SASQTKNYA KDPRHLVLL 
      RGFSLKERP LSPPAGTAT SAQTQISNT TIPITSLPS 
      PLASNDSPI SSSSSANSG VPSGVGPSP VYRPPSAQG 
      MVNARGAMM GNNPMNIPQ LPAPPGYHQ ANRPRWPAM 
      LPPTQPRSY LPPNQQVNT TQSSALIAQ LTQPPNSMP 
      GTGVNQFGQ MGNNSPLNS QQQQPQMKI NIMNQQNQQ 
      PNVQTSMSQ NPNSGIQTT SIAQVSQPN QAGTSPGQS 
      TPQPSQPLG PGRERPSIW QGLLEWIEK PKNPTDQQK 
      ITKHVPCQV SANSKDGEP ELKADGWPS KLIMQLMPK 
      QLIGNIGGA YLKNSKSVL FHPQPCEAL ESLTKVMSS 
      GFAGCVHFT STATPATCD IKVLILLYT AEKRAYLGF 
      IPNDQGAFV DRLRKVIQQ QKSTQIMRQ TQGGAGQPM 
      NPQTSGALT MGGTPPQMG GSNPVTSQT MMSQTNNMA 
      LGGGQITQN IVTSSAQAG ILGQGPRLR MQQNIQQGM 
      GPQSSQSAP NQNMAPGMM GTPPQRAPF ENQLQVERQ 
      QNLEKINQL KQTLEAAQQ QEQQYKSQL ERISHMKTS 
      QLQEALQIC QQNEMKYKI LDQQQRMAN QNNPQAGSA 
      QQQQRMMRP VMGANPGLR HLLQQQPQY RQQLMNMQQ 
      MAGNGPRPQ MNQQMQNSG NNQQSGFDE VSYDFM
    CDS sequence
    • >TC008353 RA
      atggttgtg tgtccagct gatcatggc ttacaagcc 
      gatatcgtt tttgttatc gagggaact gcagtgaat 
      ggtgcttac ataaatgac ctaaaaacc aattatatc 
      atcccttcg ttagaatat tttagtcag gggagtatt 
      gatgagagc agcttccta tcagaaggg attaattct 
      tattacgga attgtggtg tatcaggct gctgattgc 
      ttaccgcac cccagtagc gacacaatt gggcccttt 
      gtaagcccc agtaaggta ttaaatgcc atagacaaa 
      cttgaactt ataggaggt aaaggcgag tctcatgca 
      aacattgct gaaggttta gccacaacc cttcagtgt 
      tttgacgaa ttgcagcaa aaacgtgac aataacggc 
      aacattcaa aagcattgt attctagtg tgtaactcg 
      cccccttat tctctaggg gtttttgaa actcatgct 
      tacacaggg aagacagct gagcaacta gcagctatt 
      ttgcaagaa aaaaatatc aatttgtcg attatctcg 
      cctcgcaaa atcccctcg ttgtataaa ctattcgag 
      aaagccggc ggtgatttg agcgcttcc cagacaaaa 
      aactacgcc aaggacccc cgtcacttg gtcctcctg 
      cgcggcttc agcctgaag gagcgcccc ctgagcccc 
      cctgccggt accgccact tccgcccaa acccaaatt 
      tctaacact accattcct ataacctct ctccccagc 
      cccttagcc agcaatgac agccccatc tcgtcatcc 
      tcgtccgcc aactccggg gttccctca ggagtgggc 
      ccatcgccc gtctatcgg ccgcccagc gctcaaggt 
      atggtgaac gcccgaggg gccatgatg ggcaacaac 
      cccatgaac atcccgcaa ttgcccgca ccgccgggc 
      taccaccaa gcgaaccgg cccagatgg ccagctatg 
      ttgccccca acgcagccc aggtcgtat ctgccgccc 
      aaccaacag gttaatacc acccaaagt agcgcactg 
      attgcacag ctgacacaa cccccgaat tctatgccg 
      gggactggg gttaatcaa tttggacaa atggggaat 
      aattcgccg cttaattcg caacagcaa caaccccaa 
      atgaaaatt aatattatg aatcagcaa aatcagcaa 
      ccaaatgtt cagacgtcg atgtcgcag aatcccaac 
      agtgggata cagaccacc tcaattgcg caggtgtcg 
      cagccgaat caggccggt acttcccct ggacagagc 
      actcctcaa ccatctcaa ccacttggc cccggccga 
      gagcgtccc tcaatctgg caaggtctc ctcgaatgg 
      atcgaaaaa cccaaaaat ccaaccgac caacaaaaa 
      atcacgaaa catgtccca tgtcaagtc tcagcgaat 
      tcaaaagac ggcgaacct gaattaaaa gccgatggt 
      tggccctca aaactaata atgcaactc atgcccaaa 
      caactgatt ggcaacata gggggcgcc tatttaaaa 
      aattcaaaa tcggttttg ttccacccc caaccttgc 
      gaagcttta gaatctcta actaaagtc atgagttcc 
      ggctttgcc ggttgtgtg cattttaca agcaccgca 
      acccccgcc acttgtgat atcaaagtt ttgatttta 
      ctatacact gccgaaaag cgtgcttat ctcgggttt 
      atccccaac gatcaaggc gcctttgtt gaccgatta 
      cgcaaagtg atccaacaa cagaaatcc acccaaatt 
      atgcgccaa acacaaggc ggtgccggt caacccatg 
      aacccccaa acttccggt gctctcacc atgggtggc 
      acaccccca caaatgggc ggaagcaac cccgtgacg 
      tcacaaacc atgatgtct caaaccaac aacatggct 
      ttgggtggg ggacaaatc acccaaaat attgtcact 
      tcaagtgcc caagctggg attttggga cagggaccc 
      aggttaagg atgcagcag aacatacag caggggatg 
      gggccacag tcatcgcag tcagccccc aatcagaac 
      atggccccc ggaatgatg ggcacaccg cctcagagg 
      gcccccttt gagaatcag ttgcaagtg gaaaggcag 
      cagaattta gagaagatt aatcagttg aaacagacg 
      ctggaagca gctcagcaa caggaacaa cagtataaa 
      agccagttg gaaaggata agtcatatg aagacgtca 
      cagctacaa gaggcactc cagatatgt caacagaat 
      gaaatgaaa tacaaaatt cttgatcag caacaacga 
      atggccaat caaaataat ccacaagct ggaagtgcg 
      caacaacaa caaagaatg atgagacct gtgatgggg 
      gctaatcct ggcttaaga cacttgtta caacagcaa 
      ccccaatac agacagcaa ctgatgaat atgcaacaa 
      atggcaggc aacggacca cgcccacaa atgaaccaa 
      caaatgcaa aactcgggg aacaaccaa cagtccggg 
      tttgacgaa gtctcgtat gattttatg taa
    mRNA sequence
    • >TC008353 RA
      ctaaaaatt tgacaggtg tttccaata tggcggcgt 
      ttggtgctt gtatcaaat tgtttcatt aaaattgtt 
      taatatttt gactaaaca atggttgtg tgtccagct 
      gatcatggc ttacaagcc gatatcgtt tttgttatc 
      gagggaact gcagtgaat ggtgcttac ataaatgac 
      ctaaaaacc aattatatc atcccttcg ttagaatat 
      tttagtcag gggagtatt gatgagagc agcttccta 
      tcagaaggg attaattct tattacgga attgtggtg 
      tatcaggct gctgattgc ttaccgcac cccagtagc 
      gacacaatt gggcccttt gtaagcccc agtaaggta 
      ttaaatgcc atagacaaa cttgaactt ataggaggt 
      aaaggcgag tctcatgca aacattgct gaaggttta 
      gccacaacc cttcagtgt tttgacgaa ttgcagcaa 
      aaacgtgac aataacggc aacattcaa aagcattgt 
      attctagtg tgtaactcg cccccttat tctctaggg 
      gtttttgaa actcatgct tacacaggg aagacagct 
      gagcaacta gcagctatt ttgcaagaa aaaaatatc 
      aatttgtcg attatctcg cctcgcaaa atcccctcg 
      ttgtataaa ctattcgag aaagccggc ggtgatttg 
      agcgcttcc cagacaaaa aactacgcc aaggacccc 
      cgtcacttg gtcctcctg cgcggcttc agcctgaag 
      gagcgcccc ctgagcccc cctgccggt accgccact 
      tccgcccaa acccaaatt tctaacact accattcct 
      ataacctct ctccccagc cccttagcc agcaatgac 
      agccccatc tcgtcatcc tcgtccgcc aactccggg 
      gttccctca ggagtgggc ccatcgccc gtctatcgg 
      ccgcccagc gctcaaggt atggtgaac gcccgaggg 
      gccatgatg ggcaacaac cccatgaac atcccgcaa 
      ttgcccgca ccgccgggc taccaccaa gcgaaccgg 
      cccagatgg ccagctatg ttgccccca acgcagccc 
      aggtcgtat ctgccgccc aaccaacag gttaatacc 
      acccaaagt agcgcactg attgcacag ctgacacaa 
      cccccgaat tctatgccg gggactggg gttaatcaa 
      tttggacaa atggggaat aattcgccg cttaattcg 
      caacagcaa caaccccaa atgaaaatt aatattatg 
      aatcagcaa aatcagcaa ccaaatgtt cagacgtcg 
      atgtcgcag aatcccaac agtgggata cagaccacc 
      tcaattgcg caggtgtcg cagccgaat caggccggt 
      acttcccct ggacagagc actcctcaa ccatctcaa 
      ccacttggc cccggccga gagcgtccc tcaatctgg 
      caaggtctc ctcgaatgg atcgaaaaa cccaaaaat 
      ccaaccgac caacaaaaa atcacgaaa catgtccca 
      tgtcaagtc tcagcgaat tcaaaagac ggcgaacct 
      gaattaaaa gccgatggt tggccctca aaactaata 
      atgcaactc atgcccaaa caactgatt ggcaacata 
      gggggcgcc tatttaaaa aattcaaaa tcggttttg 
      ttccacccc caaccttgc gaagcttta gaatctcta 
      actaaagtc atgagttcc ggctttgcc ggttgtgtg 
      cattttaca agcaccgca acccccgcc acttgtgat 
      atcaaagtt ttgatttta ctatacact gccgaaaag 
      cgtgcttat ctcgggttt atccccaac gatcaaggc 
      gcctttgtt gaccgatta cgcaaagtg atccaacaa 
      cagaaatcc acccaaatt atgcgccaa acacaaggc 
      ggtgccggt caacccatg aacccccaa acttccggt 
      gctctcacc atgggtggc acaccccca caaatgggc 
      ggaagcaac cccgtgacg tcacaaacc atgatgtct 
      caaaccaac aacatggct ttgggtggg ggacaaatc 
      acccaaaat attgtcact tcaagtgcc caagctggg 
      attttggga cagggaccc aggttaagg atgcagcag 
      aacatacag caggggatg gggccacag tcatcgcag 
      tcagccccc aatcagaac atggccccc ggaatgatg 
      ggcacaccg cctcagagg gcccccttt gagaatcag 
      ttgcaagtg gaaaggcag cagaattta gagaagatt 
      aatcagttg aaacagacg ctggaagca gctcagcaa 
      caggaacaa cagtataaa agccagttg gaaaggata 
      agtcatatg aagacgtca cagctacaa gaggcactc 
      cagatatgt caacagaat gaaatgaaa tacaaaatt 
      cttgatcag caacaacga atggccaat caaaataat 
      ccacaagct ggaagtgcg caacaacaa caaagaatg 
      atgagacct gtgatgggg gctaatcct ggcttaaga 
      cacttgtta caacagcaa ccccaatac agacagcaa 
      ctgatgaat atgcaacaa atggcaggc aacggacca 
      cgcccacaa atgaaccaa caaatgcaa aactcgggg 
      aacaaccaa cagtccggg tttgacgaa gtctcgtat 
      gattttatg taattcttt aaaatgtat attgaaaca 
      aaatttgta acaacacta aaatattta aaaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY